LOCUS       MR218530                8748 bp    DNA     circular  SYN 
DEFINITION  Sh3tc2 (Myc-DDK-tagged) - Mouse SH3 domain and tetratricopeptide repeats 2 (Sh3tc2)
ACCESSION   NM_172628
VERSION     NM_172628.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4905..4996 
                     /label="Myc-DDK"  
     misc_feature    5026..5611 
                     /label="PolyA signal"  
     misc_feature    5083..5102 
                     /label="Restriction Enzyme"  
     misc_feature    5887..6559 
                     /label="ColE1"  
     misc_feature    6989..7783 
                     /label="Kan/Neo"  
     misc_feature    7818..8120 
                     /label="SV40 ori"  
     misc_feature    8206..8683 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4887..4893 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4887 
                     /label="Sh3tc2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTGGCTGC TTCTGTATCC CTGGGGAGCG GAGTCTACCC TGGGGTCCAG 
     1081 GTAAAGAAGG TTCTTCCAAG GATCCACCTG GATTGGCTGA GGACACGTCC TCACTTGAAA 
     1141 ACAAGAGAAA ATGTTTCCTG CCACAGAATA TGACCCAAGA CCTGGTGCTC TCATTCTGTG 
     1201 TGAAGAGTCG CTCCAGGAGG TGTGTCAACG CAGCCTTACA GGAAGCTGCA CGGAGACGGC 
     1261 TCTGGGCCCT GGAGAATGAA GCCCAGGAAG TCCACGCGCT ATTTAAGGAT CTCTCTGCAA 
     1321 GGTTGGTGAG TGTCCAGTCC CAGAAGGACC AGTTCCTCAT CACCTTCAAG ACCCTGGAGG 
     1381 AAATCTGGAA GTTTTCCACG TACCTGAACT TAGGCTATGT GTCCATATGT CTGGAACATC 
     1441 TCTTCTTTGA CCACACGTAC TGGCTCAACA GCAGGCTGGT GGACGACACA GAGATCCAAG 
     1501 TGTCTGTGGA TGACAATCAC TTGGAAAACA TATACCTGGG CCTCCTGCTG CAGGAAGGTC 
     1561 ACTTCTTCTG CAGAGCCGTG TGCTCCGTGG CTCAGCCAGC TGACAAGGAA GGAGAATATT 
     1621 TGACACTGTG CAAGAATGAG CTGATCTCCG TGCTCTCTGG AGGCGAGTCG GAGTGCGAAG 
     1681 CCATGTCCTT AGTGACGGGC CAGAGGGGCC TGGTGCCCAT GTCAGCCCTG GAGCCCCTGC 
     1741 CTGTCCCTTT CCACCAATGG TTCCTAAAGA ATCACCCAGG AATCTGTGGC CTTCCCAGGA 
     1801 AGAGAGACTG GACAGGCTCC GGCCAGATCG GCAGAGGACG GTGCAAGGCT CTGATGGATT 
     1861 ATGAGCAGGA GGAAAGAGAC GAACTGTGTT TCCTCCAGGG TGAAAGCATT GACGTCATCG 
     1921 GCTTTGTCAT ACCTGGGCTG CAGTGGTTCA TTGGGAAGTC AGTGAGTTCA GGAGAAGTGG 
     1981 GCTTTGTCCC CACCAGGAGC ATCGATCTTG ATTCTTGCTC CCCGATGAGC AAGAACTCTG 
     2041 CCTTTTTCAG TGATGAGGAA AGGAGTTCTC TCTGGTCCCC AGGAAGTGAG AGGAAGGCCG 
     2101 AGTGTTCCGG CTTCCTATGT TCGCTCGCTC ATACTGACAT CACTTCTATT TACCGGCTGA 
     2161 GTGAGTTTGA AGCCATCCAG AATCTCCAGA ATGATCTGAG TGCATCGCAG CCCGAGGGCT 
     2221 TCAGGGAGGC CAGGTCTGGT GGGACCTGGA TGGAGCGGCA GACCATAGGC TCTAGGCGTT 
     2281 CCAGCGGTTC TGGAGACTCC AGCCCCGAGG AGGATGAGCT CATCTCAGCC TCCTCGGACA 
     2341 GCTATCACCT TCCGGAGCCC GAGGACCTCG ATGACCCGGA ACTGTTCATG GACCTGAGCA 
     2401 CCAGCCTGGA GGAGGATGAT GTGGAGCACT TTGCCCCCAT CTTGGCTTTT CTAGATCACG 
     2461 AAGGCTACAC CGACCACTTT AAGAGCCTCT ATGATTTCTC CTTCTCTTTT CTGACTTCCT 
     2521 CCTTCTACAG CTTCTCTGAG GAGGATGAGC TAGTGGCCTA CCTAGAGACT TCAAGGAAGT 
     2581 GGGCCAAGAT GAGCCACATG ACATGGGCCC ATGCCCGGCT CTGCTTCCTC CTGGGCCGAC 
     2641 TAAGCATCAG GAAGACCAAA CTTTCTCAGG CCAGGGTCTA CTTTGAGGAG GCCATTCGAG 
     2701 TCCTTGATGG GGCGTTTGAG GACCTCTCCC TGGTGGCTGC TCTGTACATC AACTTGGCAG 
     2761 CTATCTACCT GAGGCAGAGG CTAAGACATA AAGGGCCCAC CTTGCTTGAA AAGGCGGGCG 
     2821 CTCTGCTGGC CTGCCTGCCA GACCATGAGT TCAGTACGAA GAATGAGCTA GATGTAGTGA 
     2881 CTTACGTGCT GCGCCAAGGG ATCGTGGTGG GGAGTGGCCT TCTGGAGGCC AGGTCCTGCT 
     2941 TCCTGGCCAT CCGGTTGCTC CTGAGCCTTG GCCGGCACGA GGAAGTTGTG CCATTCGCAG 
     3001 AACGTCTGCA GCTCCTTTCT GGACACCCTC CCGCTTCGGA GGCTACAGCC ACCATGTTGA 
     3061 GTTCTCTGTA TGACAAGAAG TATCTTCCGC ATCTCGCAGT GGCCTCTGTT CAAAAACGTG 
     3121 GTCCCCAGAG TGCCCGGGGG ATGTCTCTTT CCATTTGGCA GGCCTACTTG GTTCTCCAGA 
     3181 ATGCCACTAA GATCCTGGGT GTTCCTTCTT CAAACTGGTG TGAGGTTTCC GCCCTGGCCT 
     3241 GCCCAACCCT CAGGCAGGCC CTGGCTGCCT GTGAGGAACT GAGTGACCAG GACATCCAGA 
     3301 GGACCTTGTG TCTCATCCTG TCTAAGATGT ACCTCCAGCA CCAATCTCCT GATGGTTCTG 
     3361 TCCACTACCT GAGCCAGGCC CATGTGTTAG GGAAACTACT GGGTGAAGAG GAAGCCTTCG 
     3421 AATCTTCTCT CTGTCTGGCA TGGGCATACC TCTTAGGCAG GCAGACAGAG AAGGCCTTGG 
     3481 AGATTCTTGA GCCGCTACTA TGCTCCTTGA GAGAGACAGA GTGCGTGACC CAGAGGGGAG 
     3541 TGGTCCACAA CCTCCTGGGA CTTGCGTTTG AAGATGAAGG CCGGACTAGC AGGGCGGCCA 
     3601 AGAGCTACCT CCGAGCTCTG ATCAGAGCTC GGGAGATGGG GAACATACGT AACCAGGCTG 
     3661 TGTCTATGGC CAATCTTGGT CACTTGACCC TCAAGTCATG TATGCAGCAG TCAGCCAGGG 
     3721 GTTATCTCCT GCAGGCTGTC CGGCTGTATT CCGAACTCCA AGCCAGCAAG GAGACAGACA 
     3781 TGGAGTTGGT ACAGGTGTTA CTCTGGTTGG GCCAAGCTTC GGTGTCTGGA CACCAGCTGG 
     3841 TCCACAGCCG CCTTTGTTAT GAAATGGCAT TGCTGTTTGG CTTAAGGCAC CAACACCTAA 
     3901 GCAGTCAACT TCAGGTCACC AAATCCCTCT GCCATTTCTA CAGCTCGGTG TCCCCGAACC 
     3961 CCGATGCGTG CATTACCTAT CATGAACACT GGCTGGCCCT GGCTCAGCAA CTCAGGGATC 
     4021 GGGAGATGGA GGGACAGTTA CTGGAGTCCC TTGGGCAGCT TTATAGGAAC CTAAACACAT 
     4081 CCAGGTCCCT CCGAAGGTCC CTCGCCTGCA TCAAGGAGAG CCTGCGTATA TTCGTCGACC 
     4141 TAGGGGAGAG AGACAAGGCT GCTGAGGCCT GGCTGGGGGC CGGACGCCTG CACTACCTCA 
     4201 TGCAGGAAGA TGAGCTGGTA GAACTGTATT TGCAGGAGGC CATCCAGACA GCCTTGAGGT 
     4261 CAGAAGAGCC CTCCCTTGCT CTCAAGCTTT ACGAAGAAGC AGGTGACGTG TTCTTCAACG 
     4321 GCACCCGCCA TAGGCATCGT GCTGTGGAGT ACTACCGGGC TGGCGCTGTT CCTTTAGCGA 
     4381 GGAGAATGAA GGCGCTGAGA ACCGAGCTGC GGATCTTCAA CAAGCTGACA GAGCTGCAGA 
     4441 TAAGCCTCGA GGGCTATGAG AAAGCTTTGG AGTTTGCCAC CCTGGCTGCC AGGCTGAGTG 
     4501 TCCTCACGGG AGATCAGAAA CAGGAGCTGG TGGCTTTTCA CCGCCTGGCG ACTGTGTACT 
     4561 TCTCTCTGAA CATGTATGAG ATGGCTGAAG ACTGCTACCT GAAGACTCTG TCCCTGTGCC 
     4621 CACCATGGCT GCAGAGTCCC AAGGAGGCGC TGTACTATGC CAAGGTCTAT TGTCGCCTTG 
     4681 GCCGACTCAC TTTCTACCAA CTGAAGGATG CCCATGATGC TACTGAATAT TTTCTGCTGG 
     4741 CGCTAGCAGC TGCGGTCCTG ATGGGTGACG AGGAGCTGCA GAACACCATT AAGAACAGGC 
     4801 TAGACAGCAT CTGCCAGAGT CCCCTGTGGC ACAGCAATCC CTTCGGGTGC TCTTCAGAGA 
     4861 GGGCACGGTG GCTCAGTGGT GGGAGCCTGG CCCTCACGCG TACGCGGCCG CTCGAGCAGA 
     4921 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4981 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5041 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5101 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5161 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5221 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5281 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5341 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5401 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5461 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5521 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5581 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5641 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5701 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5761 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5821 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5881 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5941 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6001 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6061 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6121 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6181 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6241 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6301 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6361 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6421 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6481 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6541 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6601 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6661 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6721 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6781 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6841 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6901 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6961 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7021 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7081 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7141 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7201 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7261 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7321 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7381 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7441 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7501 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7561 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7621 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7681 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7741 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7801 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7861 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7921 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7981 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8041 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8101 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8161 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8221 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8281 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8341 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8401 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8461 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8521 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8581 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8641 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8701 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//