LOCUS       MR218150                8124 bp    DNA     circular  SYN 
DEFINITION  D130043K22Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA D130043K22 gene (D130043K22Rik)
ACCESSION   NM_001081051
VERSION     NM_001081051.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4281..4372 
                     /label="Myc-DDK"  
     misc_feature    4402..4987 
                     /label="PolyA signal"  
     misc_feature    4459..4478 
                     /label="Restriction Enzyme"  
     misc_feature    5263..5935 
                     /label="ColE1"  
     misc_feature    6365..7159 
                     /label="Kan/Neo"  
     misc_feature    7194..7496 
                     /label="SV40 ori"  
     misc_feature    7582..8059 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4263..4269 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4263 
                     /label="D130043K22Rik"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGTCCCCA CCAGGAGTAC TGTCTTCATT GCTGCTGCTG GCAGCCATGG 
     1081 CAGGAGGCAG TTCTCAGCAG TGCTCTGAGG GCAGGACTTA CTCCGATGCC ATCATTTCAC 
     1141 CTAACCCGGA AACCATCAGA ATCATGCGGG TGTCTCAAAC CTTCTCCGTG GGAGACTGCA 
     1201 CGGCCGCTTG CTGTGACTTG CTCACCTGTG ACCTGGCCTG GTGGTTTGAG GGCAGCTGCT 
     1261 ATCTGGTGAA ATGCATGCGC TCGGAGAATT GCGAGCCCAG GACCACAGGC CCCATCCGGT 
     1321 CTTACCTCAC TTTCGTGCGC AGACCTGTCC AGAGGCCCGG GCAGCTGCTG GACTATGGAG 
     1381 ACATGATGCT GAGCAGGGGC TCCCCCTCAG GAGCTTGGGG AGACTCCCTT GAGGACCTCA 
     1441 GGAAGGACTT GCCCTTTCTT GGCAAAGACG GGGGACCAGA GGAGACCACT GAGTACTCAG 
     1501 ATGAATACAA AGACCTGGAG CGGGGCCTCC TGCAGCCCTC CAACCAGCAA GACCCTAGAG 
     1561 GTAGCGCAGA GTACCCCGAC TGGAGCCTGC TGCCCAGCAA CGAGGGAGGT TTCAATGCTA 
     1621 CAGCTACAGG AGACAACTCA GCTGCTTCCA TGGAGAAGCT GCAGGATCCC ACGCCCCACC 
     1681 CACTGGACCA GGAGCAGCTG CAGGCCCTGA ATGAGTCGAC TTGGTCCCCT ACACCCGGGC 
     1741 ACTCTTCAAT AAGCAGTGTG TGGCCTTCCT CTGCGAGCCC ATTACCTACA GAGGAGGGAC 
     1801 TGGAAGGAGA AGAGACTTTA CAGCTCCAAG AACAACCGAG CAATAGCTCT GGAAAAGAGG 
     1861 TTCCAATGCC TTCCCATAAT CCTTCCCCTG CCAGCCTGGA GTCTAGCCCA GCCACGACGG 
     1921 AAAAAAACTC CAACTTTACA GTCACGCCAC GGAGCAGAAA ACACAGCACC CCAACTTTTC 
     1981 CTACCAGTAC AGTCCTCACT GGGCTAACCC CTCCTCCGTG GCCCTTGTCA CCTACTGCTT 
     2041 CCAGGACAGT AAAGGCGCTT GCTGTGTCTG CTGGAGATAA CCTAGTACTA ACCTTACCGG 
     2101 ACAGAGAAGC AGAACTGAAG GCCTCTGTTG AACCAGCGCC CCCTGCAGAT ACAACTTACT 
     2161 CCTATGAATG GAGTTTAATG AGCCACCCAG TAGACTTCCA AGGTAAAATC AAACAAGAAA 
     2221 ACAAGCCGAC TCTTCACCTC TCTCAATTAT CTGTGGGACT CTATGCCTTC AGAGTGGCTG 
     2281 TTTCTAGTGA GAATGCATTT GGAGAAGGCT ATGTCAATGT CACGGTTATG CCAGCTGCAA 
     2341 GAGTCAACCA GCCACCTGTA GCTGTTGTTT CTCCCCAGAC ACAAGAGCTC AGTGTGCCTT 
     2401 TGACCTCAGC CCTGATCGAT GGCAGCCAAA GTACAGATGA TACTGAAATA GTGAGTTACC 
     2461 ACTGGGAAGA AGTCGACGGG CCCTTCCTAG GAGAGGAGTT TCCAGCTGAC ACCCCAATCT 
     2521 TACGATTGTC GAACCTAGTT CCTGGTAACT ACACCTTTAG GTTGACCATC ACAGACTCGG 
     2581 ATGGCGCTAC CAACTCCACC ACTGCATCCC TCGTCATCCG TGATGCCGTG GACTACCCAC 
     2641 CCGTTGCCAA TGCAGGGCCA AATCAGACCA TCACTTTGCC CCAAAACACC ATCATTCTGA 
     2701 ATGGGAACCA GAGCAGTGAT GATCACCAGA TTGTTCTCTA TGAGTGGTTC GCAGGCCCTG 
     2761 GCGGTGAGAG CAAAGAGATG GTCATGCAGG GAGCGCAGAC CCCATACCTT CACTTGTCTG 
     2821 AGCTGCAGGA AGGAGAGTAC ACATTTCAGC TGATGGTGAC GGATTCCTCG GGACAGCAAT 
     2881 CCACCGCTTT GGTCGCTGTG ACTGTCCAGG CTGAGAACAA CCAGGCTCCG GTGGCCGTGG 
     2941 CAGGCCCCGA CAAGGAGCTG GTTTTCCCGG TGCAGAGTGC TACACTGGAT GGGAGCCGGA 
     3001 GCAGTGACGA TCACGGCATT GTCTGCTATC ACTGGGAGCA CATCAGAGGC CCCAGTGCAG 
     3061 TGGAGATGGA GAATGTGGAC AAAGCCATAG CCACTGTCAC CGGCCTTCAG GTGGGTATCT 
     3121 ACCACTTCCG GTTAACCGTG AGAGACCAGC AGGGTCTGAG CAGCACGTCC ACCCTCACTG 
     3181 TGGCTGTGAA GAAGGAAAAT AACAGCCCTC CCAGAGCCCA GGCTGGTGGC AGACATGTTC 
     3241 TTATACTTCC CAATAATTCC ATTACTTTGG ATGGTTCAAG GTCTACTGAT GACCGAGGAA 
     3301 TTGTGTCCTA TCTGTGGATC CGGGATGGCC AGAGTCCAGC TGCCGGAGAT GTCATTGGAG 
     3361 GCTCTGACCA CAGGGCAGCT CTGCAGCTCA CCAATCTGGT GGAAGGCGTC TACACTTTTC 
     3421 ACTTGCTAGT CACTGACAGT CAGGGGGCCT CGGACTCAGA CGCGGCCACT GTGGAGGTGC 
     3481 TGCCAGACCC CAAGAAGGAT GGCCTGGTGG AGTTGATCCT GCAGGTTGGT GTTGAGCAGC 
     3541 TGACAGAGCA GCAGAAGGAG ACTCTAGTGA GGCAGCTGGC TGTGCTCCTG AACGTGCTAG 
     3601 ACTCAGACGT GAAGGTGTTG AAGATCCAGG CTCACACGGA TGTCAGCACT GTGATTGTAT 
     3661 TTTATGTACA GAGTGGATCA CCTTTCAAGG TTCTCAGAGC TGCTGCTGTG GCCCGGAATC 
     3721 TGCACAAGCG GCTTTCCAAG GAGAAGGAGG CTTTCCTGCT TTTCAAGGTC TTGAGGGTAG 
     3781 ACACAGCAGG GTGCCTTCTC AAGTGTTCAG GCCACGGTCA CTGTGACCCC ATCACGAAGC 
     3841 GCTGCATCTG CTCCCAGCTG TGGATGGAGA ACCTCATACA GCGGTACATG TGGGATGGAG 
     3901 AGAGCAACTG TGAGTGGAGT GTATTCTATG TGGCAGCTCT GGCATTGACA CTAACACTGC 
     3961 TAACTGGAGC TGTGTCTTGG CTGTGCATCT GCTGCTGCAG GAGACGGAAA AGGACTAAGA 
     4021 TCCGGAAGAA AACCAAGTAT ACCATCCTAG ACAGCATGGA CGAACAGGAA AGAATGGAAC 
     4081 TGAGGCCCAA GTACGGTATC AAGCACAGGA GCACGGAGCA CAACTCCAGT CTGATGGTGT 
     4141 CGGAGTCTGA GTTTGAAAGC GACCAGGACA CGCTATTCAG CCGAGAGAGG ATGGAGAGAG 
     4201 GAGTCCTGAA GGGTTCCCTG AATGGCTGTG CCAGAAATGG AGTTTCCTTC GGTTACTACT 
     4261 CAAAGGACAG AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4321 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4381 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4441 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4501 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4561 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4621 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4681 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4741 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4801 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4861 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4921 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4981 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5041 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5101 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5161 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5221 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5281 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5341 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5401 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5461 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5521 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5581 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5641 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5701 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5761 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5821 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5881 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5941 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6001 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6061 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6121 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6181 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6241 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6301 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6361 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6421 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6481 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6541 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6601 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6661 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6721 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6781 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6841 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6901 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6961 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7021 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7081 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7141 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7201 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7261 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7321 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7381 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7441 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7501 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7561 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7621 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7681 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7741 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7801 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7861 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7921 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7981 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8041 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8101 TAACAAAAAT TTAACGCGAA TTTT
//