LOCUS       MR218112                7713 bp    DNA     circular  SYN 
DEFINITION  Pum1 (Myc-DDK-tagged) - Mouse pumilio 1 (Drosophila) (Pum1), transcript variant 5
ACCESSION   NM_001159606
VERSION     NM_001159606.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3870..3961 
                     /label="Myc-DDK"  
     misc_feature    3991..4576 
                     /label="PolyA signal"  
     misc_feature    4048..4067 
                     /label="Restriction Enzyme"  
     misc_feature    4852..5524 
                     /label="ColE1"  
     misc_feature    5954..6748 
                     /label="Kan/Neo"  
     misc_feature    6783..7085 
                     /label="SV40 ori"  
     misc_feature    7171..7648 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3852..3858 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3852 
                     /label="Pum1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGTTGCA TGTGTCTTGA AGAGAAAAGC AGTGCTTTGG CAGGACTCTT 
     1081 TCAGCCCCCA CCTGAAACAT CACCCTCAAG AACCAGCTAA TCCCAACATG CCTGTTGTTT 
     1141 TGACATCTGG AACAGGGTCG CAAGCGCAGC CACAGCCAGC TGCAAATCAG GCTCTTGCAG 
     1201 CTGGAACACA CTCCAGCCCT GTCCCAGGAT CCATAGGAGT TGCAGGCCGT TCCCAGGACG 
     1261 ACGCTATGGT GGACTACTTC TTTCAGAGGC AGCATGGTGA GCAGCTTGGG GGAGGAGGAA 
     1321 GTGGTGGAGG CGGCTATAAT ACTAGCAAAC ATCGATGGCC TACAGGGGAT AACATTCATG 
     1381 CAGAACACCA GGTACGCTCT ATGGATGAAT TGAATCATGA TTTTCAAGCA CTTGCTCTGG 
     1441 AAGGGAGAGC TATGGGCGAG CTATTCCAAA GACCCAATGC ACTTGCTGTT CAGCAGTTGA 
     1501 CAGCTGCCCA GCAGCAGCAG TATGCACTTG CAGCAGCCCA CCAGCCTCAC ATCGGTTTAG 
     1561 CTCCCGCTGC GTTTGTCCCC AACCCATACA TCATCAGCGC TGCTCCCCCA GGGACGGACC 
     1621 CCTACACAGC TGGATTGGCT GCAGCAGCGA CACTCGGCCC AGCTGTGGTC CCTCACCAAT 
     1681 ATTATGGAGT CACGCCATGG GGTGTCTACC CTGCCAGTCT CTTCCAGCAG CAGGCAGCTG 
     1741 CAGCTGCAGC AGCTACAAAC TCTGCTACTC AGCAGAGCGC TCCGCAGGCC CAGCAGGGAC 
     1801 AGCAGCAGGT TCTTCGTGGA GGAGCTAGCC AACGTCCTTT GACCCCAAAT CAGAACCAGC 
     1861 AGGGACAACA AACAGATCCC CTTGTAGCAG CTGCAGCAGT GAATTCTGCT CTTGCCTTTG 
     1921 GACAAGGATT GGCTGCAGGT ATGCCAGGTT ATCCAGTCTT GGCACCTGCT GCTTACTATG 
     1981 ACCAAACTGG TGCCCTTGTG GTGAATGCAG GGGCAAGAAA TGGCCTCGGA GCTCCAGTCC 
     2041 GGCTTGTTGC TCCAGCCCCA GTCATCATTA GCTCCTCAGC TGCACAAGCA GCTGTTGCAG 
     2101 CGGCTGCAGC GTCAGCAAAC GGAGCAGCTG GTGGTCTTGC CGGAACTACA AATGGACCAT 
     2161 TTCGACCTTT AGGCACCCAG CAGCCCCAGC CCCAGCCTCA GCAGCAGCCC AGTAACAATC 
     2221 TGGCTTCCAG CTCTTTCTAT GGCAACAACT CTCTGAGCAG TAACTCCCAG AGCAGCTCCC 
     2281 TCTTCTCTCA GGGCTCTGCA CAGCCTGCCA ACACGTCCTT GGGATTCGGA AGCAGCAGTT 
     2341 CCCTTGGTGC CACCCTGGGA TCGGCCCTTG GAGGGTTTGG AACAGCAGTT GCAAACTCCA 
     2401 ACACTGGCAG TGGCTCCCGC CGTGACTCCC TGACTGGCAG CAGTGACCTT TATAAGAGGA 
     2461 CATCGAGCAG CTTGGCCCCC ATTGGACACA GTTTTTATAG CAGCCTTAGC TATTCCTCCT 
     2521 CTCCTGGACC CGTGGGCATG CCTCTCCCTA GTCAGGGACC AGGACATTCA CAGACACCAC 
     2581 CTCCTTCCCT CTCTTCACAT GGATCCTCTT CAAGCTTAAA CCTGGGAGGA CTCACAAACG 
     2641 GCAGTGGAAG ATACATCTCT GCTGCTCCCG GTGCCGAAGC CAAGTACCGC AGTGCAAGCA 
     2701 GTGCCTCCAG CCTTTTCAGC CCCAGCAGCA CCCTTTTTTC TTCCTCTCGT TTGCGATATG 
     2761 GAATGTCTGA TGTTATGCCC TCTGGCAGGA GCAGACTTCT GGAAGATTTT CGAAACAACC 
     2821 GGTACCCTAA TTTACAACTG CGGGAGATTG CTGGGCATAT AATGGAATTT TCTCAAGACC 
     2881 AGCATGGCTC TAGATTCATT CAGCTGAAAC TAGAGCGTGC CACAGCAGCT GAGCGCCAGC 
     2941 TTGTCTTCAA TGAAATCCTC CAGGCTGCGT ACCAACTTAT GGTGGATGTG TTTGGAAATT 
     3001 ACGTCATTCA GAAGTTTTTT GAATTTGGCA GTCATGAACA GAAGCTGGCT CTGGCTGAAC 
     3061 GGATTCGAGG CCATGTCCTA TCATTGGCAC TGCAGATGTA CGGCTGCCGA GTCATCCAGA 
     3121 AAGCTTTGGA GTTTATTCCC TCAGACCAGC AGAACGAGAT GGTGCGGGAG CTAGATGGCC 
     3181 ATGTCCTGAA GTGTGTGAAA GACCAGAACG GCAACCATGT GGTTCAGAAG TGCATTGAGT 
     3241 GTGTACAGCC CCAGTCTTTG CAGTTTATCA TCGATGCATT CAAGGGGCAG GTGTTTGCTT 
     3301 TGTCTACGCA CCCGTACGGC TGTAGGGTGA TCCAGAGGAT CCTAGAGCAC TGTCTCCCTG 
     3361 ACCAGACCCT CCCCATCCTG GAGGAGCTTC ACCAGCACAC AGAGCAGCTT GTGCAGGATC 
     3421 AGTATGGAAA TTATGTAATC CAACATGTAC TGGAGCATGG TCGTCCTGAG GATAAAAGCA 
     3481 AAATTGTAGC AGAGATCCGA GGCAATGTAC TTGTATTGAG TCAGCACAAG TTTGCAAGCA 
     3541 ATGTTGTGGA GAAGTGTGTT ACTCACGCCT CACGTACAGA GCGTGCTGTG CTCATTGATG 
     3601 AGGTGTGCAC CATGAACGAC GGTCCCCACA GTGCCTTATA CACCATGATG AAGGATCAGT 
     3661 ATGCTAACTA TGTGGTCCAG AAGATGATCG ATGTGGCTGA GCCGGGTCAG CGGAAGATTG 
     3721 TCATGCACAA GATCCGGCCA CACATTGCGA CGCTTCGAAA GTACACCTAT GGCAAGCACA 
     3781 TCCTGGCCAA GCTCGAGAAG TACTACATGA AGAATGGCGT GGACTTGGGG CCCATTTGTG 
     3841 GTCCCCCTAA TGGTATCATC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3901 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3961 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4021 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4081 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4141 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4201 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4261 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4321 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4381 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4441 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4501 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4561 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4621 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4681 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4741 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4801 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4861 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4921 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4981 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5041 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5101 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5161 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5221 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5281 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5341 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5401 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5461 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5521 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5581 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5641 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5701 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5761 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5821 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5881 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5941 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6001 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6061 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6121 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6181 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6241 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6301 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6361 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6421 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6481 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6541 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6601 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6661 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6721 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6781 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6841 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6901 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6961 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7021 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7081 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7141 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7201 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7261 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7321 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7381 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7441 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7501 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7561 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7621 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7681 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//