LOCUS       MR218062                8013 bp    DNA     circular  SYN 
DEFINITION  Myo1c (Myc-DDK-tagged) - Mouse myosin IC (Myo1c), transcript variant 1
ACCESSION   NM_001080775
VERSION     NM_001080775.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4170..4261 
                     /label="Myc-DDK"  
     misc_feature    4291..4876 
                     /label="PolyA signal"  
     misc_feature    4348..4367 
                     /label="Restriction Enzyme"  
     misc_feature    5152..5824 
                     /label="ColE1"  
     misc_feature    6254..7048 
                     /label="Kan/Neo"  
     misc_feature    7083..7385 
                     /label="SV40 ori"  
     misc_feature    7471..7948 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4152..4158 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4152 
                     /label="Myo1c"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCTACCGG GCATCGGCCC TGGGCAGTGA CGGGGTTCGA GTGACCATGG 
     1081 AGAGCGCCTT GACTGCCCGA GACCGGGTAG GGGTGCAGGA CTTTGTCCTG CTGGAGAATT 
     1141 TCACCAGTGA GGCTGCCTTC ATTGAGAACC TCCGGCGGCG GTTCCGGGAG AACCTCATTT 
     1201 ATACCTACAT CGGTCCTGTC CTAGTCTCTG TCAATCCCTA CCGAGACCTA CAGATCTACA 
     1261 GCCGGCAGCA TATGGAACGC TACCGTGGTG TCAGTTTCTA TGAAGTACCA CCTCATTTGT 
     1321 TTGCAGTGGC TGACACTGTA TACCGGGCAC TTCGTACTGA GCGTCGGGAC CAGGCAGTGA 
     1381 TGATTTCTGG AGAGAGTGGG GCAGGCAAGA CAGAGGCCAC CAAGAGACTG CTCCAGTTCT 
     1441 ATGCAGAGAC CTGCCCAGCC CCTGAACGGG GTGGCGCAGT GCGAGACCGC CTGTTGCAGA 
     1501 GCAACCCCGT GTTAGAGGCC TTTGGGAATG CCAAGACTCT CCGCAACGAT AACTCCAGCC 
     1561 GGTTTGGAAA GTACATGGAT GTGCAGTTTG ACTTCAAGGG TGCCCCCGTG GGAGGCCACA 
     1621 TTCTCAGTTA CCTCCTGGAA AAGTCCCGGG TGGTGCACCA AAATCACGGA GAGCGGAACT 
     1681 TCCACGTCTT TTACCAGCTA CTGGAGGGGG GCGAGGAGGA GACTCTCCGT CGGCTGGGCT 
     1741 TGGAACGGAA CCCCCAGAGC TACTTGTACC TGGTGAAGGG CCAGTGTGCC AAGGTCTCCT 
     1801 CCATCAACGA CAAGAGTGAC TGGAAGGTTA TGAGGAAGGC GCTGTCCGTC ATTGACTTCA 
     1861 CTGAGGATGA AGTGGAGGAC TTGCTCAGCA TCGTGGCCAG CGTCCTACAT CTGGGCAACA 
     1921 TCCACTTTGC TGCTGACGAG GACAGCAATG CCCAGGTTAC TACTGAGAAC CAGCTCAAAT 
     1981 ATCTGACCAG GCTCCTTGGT GTGGAAGGTA CAACACTTAG GGAAGCCCTG ACCCACAGGA 
     2041 AGATCATCGC CAAGGGGGAA GAGCTCCTGA GCCCACTGAA CCTTGAACAG GCGGCATATG 
     2101 CAAGGGATGC GCTTGCCAAG GCTGTGTACA GCCGGACATT CACCTGGCTG GTCAGAAAGA 
     2161 TCAATAGGTC ACTGGCCTCT AAGGACGCTG AGAGCCCCAG CTGGCGAAGC ACCACGGTTC 
     2221 TTGGGCTCCT GGACATTTAC GGCTTTGAAG TGTTTCAGCA TAACAGCTTC GAGCAGTTCT 
     2281 GCATCAACTA CTGCAATGAG AAGCTGCAGC AGCTCTTCAT CGAGCTGACT CTCAAGTCGG 
     2341 AGCAGGAGGA ATACGAGGCT GAGGGCATCG CGTGGGAACC TGTCCAGTAC TTCAACAACA 
     2401 AGATCATCTG TGACCTGGTA GAGGAGAAGT TCAAGGGCAT CATCTCCATC TTGGATGAAG 
     2461 AGTGCCTGCG TCCTGGGGAG GCCACGGACC TGACCTTTCT GGAGAAGTTG GAGGACACTG 
     2521 TCAAGCCCCA CCCTCACTTC CTGACGCACA AGCTCGCTGA CCAGAAGACC AGGAAATCTC 
     2581 TAGACCGAGG GGAGTTCCGC CTTCTGCATT ATGCTGGAGA GGTGACCTAC AGTGTGACTG 
     2641 GGTTTCTGGA TAAAAACAAT GACCTCCTCT TCCGGAACCT GAAGGAGACC ATGTGCAGCT 
     2701 CAATGAACCC CATCATGGCC CAGTGCTTTG ACAAGAGTGA GCTCAGTGAC AAGAAGCGGC 
     2761 CAGAGACGGT GGCCACCCAG TTCAAGATGA GCCTCCTGCA GCTCGTGGAG ATCCTGAGGT 
     2821 CTAAGGAGCC TGCCTATATC CGGTGCATCA AGCCAAACGA CGCCAAGCAG CCGGGTCGCT 
     2881 TTGATGAGGT GCTCATCCGA CATCAGGTGA AGTACCTGGG ACTGATGGAG AATCTGCGCG 
     2941 TGCGCAGAGC TGGCTTTGCC TATCGTCGCA AATATGAGGC TTTCCTGCAG AGGTACAAGT 
     3001 CACTGTGCCC AGAGACATGG CCCATGTGGG CAGGACGGCC CCAGGATGGT GTGGCCGTGT 
     3061 TGGTCAGACA CCTCGGCTAC AAGCCAGAAG AGTACAAAAT GGGCAGGACT AAGATCTTCA 
     3121 TCCGATTTCC CAAGACCTTG TTTGCCACAG AGGACTCCCT GGAAGTCCGG CGGCAGAGTC 
     3181 TAGCCACCAA GATCCAGGCG GCCTGGAGGG GCTTTCATTG GCGACAGAAA TTTCTCCGGG 
     3241 TGAAGCGATC AGCCATCTGT ATCCAGTCAT GGTGGCGTGG CACACTGGGC CGGAGGAAGG 
     3301 CAGCCAAGAG GAAGTGGGCA GCCCAGACCA TCCGTCGACT CATCCGTGGC TTCATTTTGC 
     3361 GCCATTCACC CCGGTGCCCT GAGAATGCCT TCTTCTTGGA CCACGTGCGC GCCTCATTTT 
     3421 TGCTTAACCT GAGGCGGCAA CTGCCCCGGA ATGTTCTGGA CACCTCCTGG CCCACACCCC 
     3481 CACCTGCCCT GAGAGAGGCC TCAGAACTGC TACGGGAACT GTGCATGAAG AACATGGTGT 
     3541 GGAAGTACTG CCGGAGCATC AGCCCTGAGT GGAAGCAGCA GCTGCAGCAA AAGGCGGTGG 
     3601 CTAGTGAAAT TTTCAAGGGC AAGAAGGACA ACTACCCCCA GAGTGTCCCC AGACTCTTCA 
     3661 TTAGCACACG GCTTGGCACA GAGGAGATCA GCCCCAGAGT GCTTCAATCC TTGGGCTCTG 
     3721 AACCCATCCA GTATGCCGTG CCCGTGGTAA AATACGACCG TAAGGGTTAC AAGCCTCGCC 
     3781 CCCGGCAGCT GCTGCTCACG CCCAGTGCTG TGGTCATTGT GGAGGATGCT AAAGTCAAGC 
     3841 AGAGAATTGA TTATGCCAAC CTAACCGGAA TCTCTGTCAG TAGCCTGAGT GATAGCCTAT 
     3901 TTGTGCTTCA CGTGCAGCGT GAAGACAACA AGCAGAAGGG AGATGTGGTG CTGCAGAGTG 
     3961 ATCATGTGAT CGAGACACTA ACCAAGACGG CCCTCAGTGC TGACCGCGTG AACAATATCA 
     4021 ACATCAACCA GGGCAGCATA ACGTTTGCAG GGGGTCCAGG CAGGGACGGC ATCATTGACT 
     4081 TCACATCGGG CTCAGAGCTT CTCATCACCA AGGCTAAGAA TGGCCACCTG GCTGTGGTGG 
     4141 CCCCACGGCT GAATTCTCGG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4201 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4261 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4321 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4381 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4441 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4501 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4561 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4621 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4681 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4741 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4801 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4861 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4921 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4981 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5041 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5101 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5161 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5221 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5281 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5341 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5401 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5461 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5521 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5581 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5641 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5701 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5761 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5821 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5881 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5941 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6001 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6061 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6121 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6181 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6241 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6301 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6361 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6421 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6481 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6541 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6601 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6661 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6721 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6781 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6841 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6901 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6961 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7021 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7081 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7141 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7201 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7261 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7321 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7381 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7441 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7501 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7561 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7621 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7681 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7741 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7801 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7861 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7921 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7981 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//