LOCUS       MR218010                8739 bp    DNA     circular  SYN 
DEFINITION  Atxn2 (Myc-DDK-tagged) - Mouse ataxin 2 (Atxn2)
ACCESSION   NM_009125
VERSION     NM_009125.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4896..4987 
                     /label="Myc-DDK"  
     misc_feature    5017..5602 
                     /label="PolyA signal"  
     misc_feature    5074..5093 
                     /label="Restriction Enzyme"  
     misc_feature    5878..6550 
                     /label="ColE1"  
     misc_feature    6980..7774 
                     /label="Kan/Neo"  
     misc_feature    7809..8111 
                     /label="SV40 ori"  
     misc_feature    8197..8674 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4878..4884 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4878 
                     /label="Atxn2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGTTCGTCC ACCGCCGCCG CTCAGCGGCC CGCGGCGGGG GACCCCGAGC 
     1081 CGCGCCGCCC GGCGGGCTGG GCCGCGCGGC GCTCGCTCCC GCGGACGGCG CGGCGCGGCG 
     1141 GGCGGGGCGG CGCGGTGGCG TATCCCTCCG CCGGCCCTCC CCCGCGCGGC CCCGGCGCCC 
     1201 CTCCCCGCGG GCCGCGCTCG CCACCCTGCG CCTCAGACTG TTTTGGTAGC AACGGCCACG 
     1261 GCGCGTCCCG GCCCGGCTCC CGGCGGCTGC TCGGTGTCTG CGGGCCTCCC CGCCCCTTCG 
     1321 TCGTTGTCCT GCTGCCTCTG GCCCCCGCGG CCACGCCGGC CCGCGCCTGC CCGCCCGGCG 
     1381 TCCGCGCGTC CCCGCCGCGC TCCGGCGTCT CCTCCTCGGC GCGCCCGGCA CCCGGCTGTC 
     1441 CCCGCCCGGC GTGCGAGCCG GTGTATGGGC CGCTCACCAT GTCGCTGAAG CCGCAGCCGC 
     1501 AGCCGCCCGC GCCCGCCACT GGCCGCAAGC CCGGCGGCGG CCTGCTCTCG TCGCCCGGCG 
     1561 CCGCGCCGGC CTCGGCCGCG GTGACCTCGG CTTCCGTGGT GCCGGCCCCG GCCGCGCCGG 
     1621 TGGCGTCTTC CTCGGCGGCC GCGGGCGGCG GGCGTCCCGG CCTGGGCAGA GGTCGGAACA 
     1681 GTAGCAAAGG ACTGCCTCAG CCTACGATTT CTTTTGATGG AATCTATGCA AACGTGAGGA 
     1741 TGGTTCATAT ACTTACGTCA GTTGTTGGAT CGAAATGTGA AGTACAAGTG AAAAACGGAG 
     1801 GCATATATGA AGGAGTTTTT AAAACATACA GTCCTAAGTG TGACTTGGTA CTTGATGCTG 
     1861 CACATGAGAA AAGTACAGAA TCCAGTTCGG GGCCAAAACG TGAAGAAATA ATGGAGAGTG 
     1921 TTTTGTTCAA ATGCTCAGAC TTCGTTGTGG TACAGTTTAA AGATACAGAC TCCAGTTATG 
     1981 CACGGAGAGA TGCTTTTACT GACTCTGCTC TCAGCGCAAA GGTGAATGGT GAGCACAAGG 
     2041 AGAAGGACCT GGAGCCCTGG GATGCAGGGG AGCTCACGGC CAGCGAGGAG CTGGAGCTGG 
     2101 AGAATGATGT GTCTAATGGA TGGGACCCCA ATGACATGTT TCGATATAAT GAAGAGAATT 
     2161 ATGGTGTGGT GTCCACATAT GATAGCAGTT TATCTTCATA TACGGTTCCT TTAGAAAGGG 
     2221 ACAACTCAGA AGAATTTCTT AAACGGGAGG CAAGGGCAAA CCAGTTAGCA GAAGAAATTG 
     2281 AATCCAGTGC TCAGTACAAA GCTCGTGTCG CCCTTGAGAA TGATGACCGG AGTGAGGAAG 
     2341 AAAAATACAC AGCAGTCCAG AGAAACTGCA GTGACCGGGA GGGGCATGGC CCCAACACTA 
     2401 GGGACAATAA ATATATTCCT CCTGGACAAA GAAACAGAGA AGTCCTATCC TGGGGAAGTG 
     2461 GGAGACAGAG CTCACCACGG ATGGGCCAGC CTGGGCCAGG CTCCATGCCG TCAAGAGCTG 
     2521 CTTCTCACAC TTCAGATTTC AACCCGAACG CTGGCTCAGA CCAAAGAGTA GTTAATGGAG 
     2581 GTGTTCCCTG GCCATCGCCT TGCCCATCTC CTTCCTCTCG CCCACCTTCT CGCTACCAGT 
     2641 CAGGTCCCAA CTCTCTTCCA CCTCGGGCAG CCACCCCTAC ACGGCCGCCC TCCAGGCCCC 
     2701 CCTCGAGGCC CTCCAGACCC CCGTCTCACC CCTCTGCTCA TGGTTCTCCA GCTCCTGTCT 
     2761 CTACTATGCC TAAACGCATG TCTTCAGAAG GACCCCCAAG GATGTCTCCA AAGGCACAGC 
     2821 GCCACCCTCG GAATCACAGA GTCTCTGCTG GGAGAGGCTC CATGTCTAGT GGCCTAGAAT 
     2881 TTGTATCCCA CAATCCCCCA AGTGAAGCAG CTGCTCCTCC AGTGGCAAGG ACCAGTCCTG 
     2941 CAGGGGGAAC GTGGTCCTCA GTGGTCAGTG GGGTTCCAAG GTTATCTCCC AAAACTCACA 
     3001 GACCCAGGTC TCCCAGGCAG AGCAGCATTG GAAACTCTCC CAGCGGGCCT GTGCTTGCTT 
     3061 CTCCCCAAGC TGGCATCATC CCTGCAGAAG CCGTTTCCAT GCCTGTTCCC GCCGCATCTC 
     3121 CGACTCCTGC CAGCCCTGCA TCCAACAGAG CACTGACCCC ATCTATTGAG GCAAAAGATT 
     3181 CCAGGCTTCA AGATCAGAGG CAGAACTCTC CTGCAGGGAG TAAAGAAAAT GTTAAAGCAA 
     3241 GTGAAACATC ACCTAGCTTT TCAAAAGCTG ACAACAAAGG TATGTCACCA GTTGTTTCTG 
     3301 AACACAGAAA ACAGATTGAT GACTTAAAGA AGTTTAAGAA TGATTTTAGG TTACAGCCAA 
     3361 GCTCTACATC TGAATCTATG GATCAACTAC TAAGCAAAAA TAGAGAAGGA GAAAAGTCAC 
     3421 GAGATTTGAT TAAAGATAAA ACGGAAGCAA GTGCTAAGGA TAGTTTCATT GACAGCAGCA 
     3481 GCAGCAGCAG CAACTGTACC AGTGGCAGCA GCAAGACCAA CAGCCCTAGC ATCTCCCCTT 
     3541 CCATGCTTAG TAATGCAGAG CACAAGAGGG GGCCTGAGGT CACATCCCAA GGGGTGCAGA 
     3601 CTTCCAGCCC AGCCTGCAAA CAAGAGAAGG ATGACAGAGA AGAGAAGAAA GACACAACAG 
     3661 AGCAGGTTAG GAAATCGACA TTGAATCCCA ATGCAAAGGA GTTCAACCCT CGTTCTTTCT 
     3721 CTCAGCCAAA GCCTTCTACT ACCCCAACGT CACCTCGGCC TCAAGCACAA CCCAGCCCAT 
     3781 CTATGGTGGG TCATCAGCAG CCAGCTCCAG TGTACACTCA GCCTGTGTGC TTCGCACCCA 
     3841 ATATGATGTA TCCCGTCCCA GTGAGCCCGG GCGTACAACC TTTATACCCA ATACCTATGA 
     3901 CGCCCATGCC TGTGAACCAA GCCAAGACAT ATAGAGCAGG TAAAGTACCA AATATGCCCC 
     3961 AACAGCGACA AGACCAACAT CATCAAAGCA CCATGATGCA CCCAGCCTCC GCGGCAGGGC 
     4021 CACCCATCGT AGCCACCCCG CCCGCTTACT CCACTCAGTA CGTTGCCTAC AGCCCTCAGC 
     4081 AGTTTCCCAA TCAGCCTTTG GTCCAGCATG TGCCGCATTA TCAGTCTCAG CATCCTCATG 
     4141 TGTACAGTCC TGTCATACAA GGTAATGCCA GGATGATGGC ACCACCAGCA CATGCTCAGC 
     4201 CTGGTTTAGT GTCTTCTTCA GCTGCTCAGT TCGGGGCTCA CGAGCAGACG CACGCCATGT 
     4261 ATGCATGTCC CAAATTACCA TACAACAAGG AGACAAGCCC TTCTTTCTAC TTTGCCATTT 
     4321 CCACCGGCTC CCTCGCTCAG CAGTATGCAC ATCCTAATGC CGCCCTGCAT CCACATACTC 
     4381 CCCATCCTCA GCCTTCGGCC ACTCCCACCG GACAGCAGCA AAGCCAGCAT GGTGGAAGTC 
     4441 ACCCTGCACC CAGTCCTGTT CAGCACCATC AGCACCAGGC TGCCCAGGCT CTTCATCTGG 
     4501 CCAGTCCACA GCAGCAGTCG GCCATTTATC ATGCGGGGCT GGCACCAACA CCACCTTCCA 
     4561 TGACACCTGC CTCTAATACA CAGTCTCCAC AGAGCAGTTT CCCAGCAGCA CAACAGACAG 
     4621 TCTTCACCAT CCACCCTTCT CATGTTCAGC CGGCATACAC CACCCCACCC CACATGGCCC 
     4681 ACGTACCTCA GGCTCATGTA CAGTCAGGAA TGGTTCCTTC TCATCCAACT GCCCATGCGC 
     4741 CAATGATGCT AATGACGACA CAGCCACCCG GCGGTCCCCA GGCCGCCCTC GCTCAAAGTG 
     4801 CACTACAGCC CATTCCAGTC TCGACAACAG CGCATTTCCC TTATATGACG CACCCTTCAG 
     4861 TACAAGCCCA CCACCAACAG CAGTTGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4921 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4981 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5041 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5101 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5161 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5221 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5281 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5341 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5401 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5461 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5521 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5581 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5641 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5701 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5761 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5821 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5881 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5941 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6001 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6061 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6121 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6181 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6241 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6301 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6361 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6421 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6481 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6541 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6601 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6661 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6721 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6781 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6841 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6901 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6961 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7021 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7081 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7141 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7201 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7261 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7321 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7381 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7441 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7501 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7561 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7621 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7681 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7741 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7801 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7861 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7921 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7981 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8041 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8101 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8161 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8221 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8281 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8341 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8401 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8461 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8521 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8581 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8641 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8701 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//