LOCUS       MR217926                7461 bp    DNA     circular  SYN 
DEFINITION  Nrg2 (Myc-DDK-tagged) - Mouse neuregulin 2 (Nrg2)
ACCESSION   NM_001167891
VERSION     NM_001167891.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3616..3707 
                     /label="Myc-DDK"  
     misc_feature    3737..4322 
                     /label="PolyA signal"  
     misc_feature    3794..3813 
                     /label="Restriction Enzyme"  
     misc_feature    4598..5270 
                     /label="ColE1"  
     misc_feature    5700..6494 
                     /label="Kan/Neo"  
     misc_feature    6529..6831 
                     /label="SV40 ori"  
     misc_feature    6917..7394 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3591..3598 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3591 
                     /label="Nrg2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGCAGGTT TGCTGCTCAG CGCTGCCGCC GCCACTGGAG AAGGCTCGGT 
     1081 GCAGCAGCTA CAGCTACAGT TACAGCGACA GCAGCAGCAG CACCACCAGC AGCAGCAGAA 
     1141 GCAGCAGCAG CAGCAGCAGC AGAAACAGCA GCAGCAGCAA CAGCAGCAGC AGCGAGAGCA 
     1201 GCGGCAGCAA CAGCGGCAGC AGCAGCATCT TCCGTCCCGC TGCGCCCCCA GAGCCGCGGC 
     1261 CGCAGCCACA GCCGCAGCCC CGCAGCCCCG CAGCCCGGAG AGCCGCCGCC CGCTCGCGAG 
     1321 CCGCAGCCGC CGGCGGCATG AGGCGCGACC CGGCCCCCGG CTTCTCGATG CTGCTCTTCG 
     1381 GTGTGTCACT CGCCTGCTAC TCGCCCAGCC TCAAGTCGGT GCAGGACCAG GCGTACAAGG 
     1441 CACCCGTGGT GGTGGAGGGC AAGGTACAGG GACTGGCCCC GGCAGGCGGT TCCAGCTCTA 
     1501 ACAGCACCCG AGAGCCGCCC GCCTCGGGTC GGGTGGCGCT GGTGAAGGTG CTGGACAAGT 
     1561 GGCCGCTCCG GAGCGGGGGG CTGCAGCGCG AGCAGGTGAT CAGCGTGGGC TCCTGTGCGC 
     1621 CGCTCGAAAG GAACCAGCGC TACATCTTTT TCCTGGAGCC CACCGAGCAG CCCTTAGTTT 
     1681 TTAAGACAGC CTTTGCCCCG GTCGACCCTA ACGGCAAAAA CATCAAGAAA GAGGTGGGCA 
     1741 AGATCCTGTG CACTGACTGC GCCACCCGGC CCAAGCTGAA GAAGATGAAG AGCCAGACAG 
     1801 GAGAGGTGGG TGAGAAGCAG TCGCTCAAGT GTGAGGCAGC GGCGGGAAAC CCCCAGCCCT 
     1861 CCTATCGCTG GTTCAAGGAT GGCAAGGAAC TCAACCGGAG TCGTGATATT CGCATCAAGT 
     1921 ATGGCAATGG CAGAAAGAAC TCACGGCTAC AGTTCAACAA AGTGAGGGTG GAGGATGCCG 
     1981 GGGAGTACGT CTGTGAGGCC GAGAACATCC TTGGGAAGGA CACCGTGAGG GGCCGACTCC 
     2041 ATGTCAACAG CGTGAGCACC ACTCTGTCAT CCTGGTCGGG ACATGCCCGG AAGTGCAATG 
     2101 AGACCGCCAA GTCCTACTGT GTGAATGGAG GCGTGTGCTA CTACATCGAG GGCATCAACC 
     2161 AGCTCTCCTG CAAATGTCCA AACGGATTCT TCGGACAGAG ATGTTTGGAG AAACTGCCTT 
     2221 TGCGATTGTA CATGCCAGAT CCTAAGCAAA AGGCTGAGGA GCTGTACCAG AAGAGAGTGC 
     2281 TGACAATTAC TGGTATCTGT GTGGCCCTGC TGGTCGTGGG CATCGTCTGT GTGGTCGCCT 
     2341 ACTGCAAGAC CAAAAAACAG AGGAGGCAGA TGCATCATCA TCTCCGGCAG AACATGTGCC 
     2401 CAGCCCACCA GAACCGAAGC CTGGCCAACG GGCCCAGCCA CCCTCGGCTG GACCCTGAGG 
     2461 AGATCCAGAT GGCAGATTAC ATCTCCAAAA ATGTGCCAGC TACAGACCAC GTGATCCGGA 
     2521 GGGAAGCTGA GACCACGTTC TCTGGGAGCC ACTCCTGTTC ACCTTCTCAC CACTGCTCCA 
     2581 CAGCCACGCC CACCTCCAGC CACAGACATG AGAGCCACAC GTGGAGCCTG GAACGTTCAG 
     2641 AGAGCCTGAC CTCGGATTCC CAGTCAGGCA TCATGCTATC ATCAGTAGGC ACCAGCAAGT 
     2701 GCAACAGCCC AGCATGTGTG GAGGCACGGG CGCGGAGGGC AGCAGCCTAC AGCCAGGAGG 
     2761 AGCGGCGCAG GGCTGCCATG CCACCCTACC ATGACTCCAT AGACTCGCTG CGTGACTCTC 
     2821 CACACAGTGA AAGGTACGTG TCAGCCTTGA CCACGCCCGC TCGCCTCTCG CCCGTGGACT 
     2881 TCCACTACTC GCTGGCCACG CAGGTGCCGA CTTTCGAGAT CACGTCGCCC AACTCTGCGC 
     2941 ATGCCGTGTC GCTGCCGCCC GCCGCGCCCA TCAGCTACCG CCTGGCGGAG CAGCAGCCGC 
     3001 TCCTGCGGCA TCCAGCGCCG CCCGGCCCGG GGCCGGGGTC GGGGCCCGGA GCGGACATGC 
     3061 AGCGCAGCTA CGACAGCTAC TACTACCCTG CGGCGGGGCC CGGGCCGCGG CGCAGCGCCT 
     3121 GCGCGCTGGG AGGCAGCTTG GGCAGCCTGC CCGCCAGCCC CTTCCGCATC CCGGAGGACG 
     3181 ACGAGTACGA GACCACGCAG GAGTGCGCGC CCCCGCCGCC GCCGCGGCCG CGCACGCGCG 
     3241 GCGCGTCCCG CAGGACGTCG GCGGGGCCGC GGCGCTGGCG GCGCTCCCGG CTCAACGGGT 
     3301 TGGCGGCGCA GCGCGCACGC GCGGCGCGGG ACTCGCTGTC ATTGAGCAGC GGTTCGGGCT 
     3361 GCGGCTCGGC GTCGGCCTCG GACGACGACG CGGACGACGC GGACGGGGCG CTGGCGGCCG 
     3421 AGAGCACGCC ATTCCTCGGC CTGCGAGCGG CGCACGACGC GTTGCGCTCG GACTCGCCGC 
     3481 CGCTGTGCCC CGCGGCCGAC AGCAGGACTT ACTACTCCCT GGACAGCCAC AGCACGCGCG 
     3541 CCAGCAGCAG ACACAGCCGG GGGCCGCCCA CGAGGGCCAA GCAGGACTCG GGGCCCCTCA 
     3601 GCGGACCGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3661 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3721 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3781 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3841 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3901 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3961 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4021 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4081 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4141 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4201 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4261 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4321 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4381 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4441 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4501 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4561 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4621 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4681 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4741 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4801 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4861 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4921 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4981 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5041 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5101 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5161 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5221 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5281 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5341 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5401 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5461 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5521 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5581 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5641 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5701 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5761 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5821 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5881 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5941 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6001 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6061 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6121 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6181 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6241 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6301 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6361 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6421 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6481 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6541 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6601 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6661 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6721 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6781 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6841 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6901 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6961 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7021 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7081 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7141 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7201 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7261 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7321 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7381 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7441 CAAAAATTTA ACGCGAATTT T
//