LOCUS       MR217804                8331 bp    DNA     circular  SYN 
DEFINITION  Man2a1 (Myc-DDK-tagged) - Mouse mannosidase 2, alpha 1 (Man2a1)
ACCESSION   NM_008549
VERSION     NM_008549.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4488..4579 
                     /label="Myc-DDK"  
     misc_feature    4609..5194 
                     /label="PolyA signal"  
     misc_feature    4666..4685 
                     /label="Restriction Enzyme"  
     misc_feature    5470..6142 
                     /label="ColE1"  
     misc_feature    6572..7366 
                     /label="Kan/Neo"  
     misc_feature    7401..7703 
                     /label="SV40 ori"  
     misc_feature    7789..8266 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4470..4476 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4470 
                     /label="Man2a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTTAAGT CGCCAGTTCA CCGTGTTTGG CAGCGCGATC TTCTGCGTCG 
     1081 TAATCTTCTC ACTCTACCTG ATGCTGGACA GGGGTCACTT GGACTACCCT CGGGGCCCGC 
     1141 GCCAGGAGGG CTCCTTTCCG CAGGGCCAGC TTTCAATATT GCAAGAAAAG ATTGACCATT 
     1201 TGGAGCGTTT GCTCGCTGAG AACAACGAGA TCATCTCAAA TATCAGAGAC TCAGTCATCA 
     1261 ACCTGAGCGA GTCTGTGGAG GACGGCCCGC GGGGGTCACC AGGCAACGCC AGCCAAGGCT 
     1321 CCATCCACCT CCACTCGCCA CAGTTGGCCC TGCAGGCTGA CCCCAGAGAC TGTTTGTTTG 
     1381 CTTCACAGAG TGGGAGTCAG CCCCGGGATG TGCAGATGTT GGATGTTTAC GATCTGATTC 
     1441 CTTTTGATAA TCCAGATGGT GGAGTTTGGA AGCAAGGATT TGACATTAAG TATGAAGCGG 
     1501 ATGAGTGGGA CCATGAGCCC CTGCAAGTGT TTGTGGTGCC TCACTCCCAT AATGACCCAG 
     1561 GTTGGTTGAA GACTTTCAAT GACTACTTTA GAGACAAGAC TCAGTATATT TTTAATAACA 
     1621 TGGTCCTAAA GCTGAAAGAA GACTCAAGCA GGAAGTTTAT GTGGTCTGAG ATCTCTTACC 
     1681 TTGCAAAATG GTGGGATATT ATAGATATTC CGAAGAAGGA AGCTGTTAAA AGTTTACTAC 
     1741 AGAATGGTCA GCTGGAAATT GTGACCGGTG GCTGGGTTAT GCCTGATGAA GCCACTCCAC 
     1801 ATTATTTTGC CTTAATTGAC CAACTAATTG AAGGGCACCA ATGGCTGGAA AAAAATCTAG 
     1861 GAGTGAAACC TCGATCGGGC TGGGCCATAG ATCCCTTTGG TCATTCACCC ACAATGGCTT 
     1921 ATCTTCTAAA GCGTGCTGGA TTTTCACACA TGCTCATCCA GAGAGTCCAT TATGCAATCA 
     1981 AAAAACACTT CTCTTTGCAT AAAACGCTGG AGTTTTTCTG GAGACAGAAT TGGGATCTTG 
     2041 GATCTGCTAC AGACATTTTG TGCCATATGA TGCCCTTCTA CAGCTACGAC ATCCCTCACA 
     2101 CCTGTGGGCC TGATCCTAAA ATATGCTGCC AGTTTGATTT TAAACGGCTT CCTGGAGGCA 
     2161 GATATGGTTG TCCCTGGGGA GTTCCCCCAG AAGCAATATC TCCTGGAAAT GTCCAAAGCA 
     2221 GGGCTCAGAT GCTATTGGAT CAGTACCGGA AAAAGTCAAA ACTTTTCCGC ACTAAAGTTC 
     2281 TGCTGGCTCC ACTGGGAGAC GACTTTCGGT TCAGTGAATA CACAGAGTGG GATCTGCAGT 
     2341 GCAGGAACTA CGAGCAACTG TTCAGTTACA TGAACTCGCA GCCTCATCTG AAAGTGAAGA 
     2401 TCCAGTTTGG AACCTTGTCA GATTATTTCG ACGCATTGGA GAAAGCGGTG GCAGCCGAGA 
     2461 AGAAGAGTAG CCAGTCTGTG TTCCCTGCCC TGAGTGGAGA CTTCTTCACG TACGCTGACA 
     2521 GAGACGACCA TTACTGGAGT GGCTACTTCA CGTCCAGACC TTTCTACAAA CGAATGGACA 
     2581 GAATAATGGA ATCTCGTATA AGGGCTGCTG AAATTCTTTA CCAGTTGGCC TTGAAACAAG 
     2641 CTCAGAAATA CAAGATAAAT AAATTTCTTT CATCACCTCA TTACACAACA CTGACAGAAG 
     2701 CCAGAAGGAA CTTAGGACTA TTTCAGCATC ATGATGCCAT CACAGGAACC GCGAAAGACT 
     2761 GGGTGGTTGT GGACTATGGT ACCAGACTCT TTCAGTCATT AAATTCTTTG GAGAAGATAA 
     2821 TTGGAGATTC TGCATTTCTT CTCATTTTAA AGGACAAAAA GCTGTACCAG TCAGATCCTT 
     2881 CCAAAGCCTT CTTAGAGATG GATACGAAGC AAAGTTCACA AGATTCTCTG CCCCAAAAAA 
     2941 TTATAATACA ACTGAGCGCA CAGGAGCCAA GGTACCTTGT GGTCTACAAT CCCTTTGAAC 
     3001 AAGAACGGCA TTCAGTGGTG TCCATCCGGG TAAACTCCGC CACAGTGAAA GTGCTGTCTG 
     3061 ATTCGGGAAA ACCGGTGGAG GTTCAAGTCA GTGCAGTTTG GAACGACATG AGGACAATTT 
     3121 CACAAGCAGC CTATGAGGTT TCTTTTCTAG CTCATATACC ACCACTGGGA CTGAAAGTGT 
     3181 TTAAGATCTT AGAGTCACAA AGTTCAAGCT CACACTTGGC TGATTATGTC CTATATAATA 
     3241 ATGATGGACT AGCAGAAAAT GGAATATTCC ACGTGAAGAA CATGGTGGAT GCTGGAGATG 
     3301 CCATAACAAT AGAGAATCCC TTCCTGGCGA TTTGGTTTGA CCGATCTGGG CTGATGGAGA 
     3361 AAGTGAGAAG GAAAGAAGAC AGTAGACAGC ATGAACTGAA GGTCCAGTTC CTGTGGTACG 
     3421 GAACCACCAA CAAAAGGGAC AAGAGCGGTG CCTACCTCTT CCTGCCTGAC GGGCAGGGCC 
     3481 AGCCATATGT TTCCCTAAGA CCGCCCTTTG TCAGAGTGAC ACGTGGAAGG ATCTACTCAG 
     3541 ATGTGACCTG TTTCCTCGAA CACGTTACTC ACAAAGTCCG CCTGTACAAC ATTCAGGGAA 
     3601 TAGAAGGTCA GTCCATGGAA GTTTCTAATA TTGTAAACAT CAGGAATGTG CATAACCGTG 
     3661 AGATTGTAAT GAGAATTTCA TCTAAAATAA ACAACCAAAA TAGATATTAT ACTGACCTAA 
     3721 ATGGATATCA GATTCAGCCT AGAAGGACCA TGAGCAAATT GCCTCTTCAA GCCAACGTTT 
     3781 ACCCGATGTG CACAATGGCG TATATCCAGG ATGCTGAGCA CCGGCTCACG CTGCTCTCTG 
     3841 CTCAGTCTCT AGGTGCTTCC AGCATGGCTT CTGGTCAGAT TGAAGTCTTC ATGGATCGAA 
     3901 GGCTCATGCA GGATGATAAC CGTGGCCTTG GGCAAGGCGT CCATGACAAT AAGATTACAG 
     3961 CTAATTTGTT TCGAATCCTC CTCGAGAAGA GAAGCGCTGT GAACATGGAA GAAGAAAAGA 
     4021 AGAGCCCTGT CAGCTACCCT TCCCTCCTCA GCCACATGAC TTCGTCCTTC CTCAACCATC 
     4081 CCTTTCTCCC CATGGTACTA AGTGGCCAGC TCCCCTCCCC TGCCTTTGAG CTGCTGAGTG 
     4141 AATTTCCTCT GCTGCAGTCC TCTCTACCTT GTGATATCCA TCTGGTCAAC CTGCGGACAA 
     4201 TACAATCAAA GATGGGCAAA GGCTATTCGG ATGAGGCAGC CTTGATCCTC CACAGGAAAG 
     4261 GGTTTGATTG CCAGTTCTCC AGCAGAGGCA TCGGGCTACC CTGTTCCACT ACTCAGGGAA 
     4321 AGATGTCAGT TCTGAAACTT TTCAACAAGT TTGCTGTGGA GAGTCTCGTC CCTTCCTCTC 
     4381 TGTCCTTGAT GCACTCCCCT CCAGATGCCC AGAACATGAG TGAAGTCAGC CTGAGCCCCA 
     4441 TGGAGATCAG CACGTTCCGT ATCCGCTTGC GTTGGACCAC GCGTACGCGG CCGCTCGAGC 
     4501 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4561 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4621 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4681 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4741 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4801 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4861 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4921 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4981 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5041 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5101 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5161 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5221 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5281 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5341 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5401 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5461 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5521 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5581 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5641 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5701 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5761 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5821 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5881 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5941 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6001 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6061 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6121 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6181 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6241 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6301 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6361 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6421 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6481 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6541 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6601 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6661 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6721 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6781 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6841 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6901 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6961 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7021 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7081 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7141 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7201 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7261 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7321 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7381 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7441 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7501 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7561 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7741 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7801 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7861 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7921 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7981 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8041 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8101 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8161 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8221 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8281 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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