LOCUS       MR217770                8892 bp    DNA     circular  SYN 
DEFINITION  Pfas (Myc-DDK-tagged) - Mouse phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) (Pfas)
ACCESSION   NM_001159519
VERSION     NM_001159519.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5049..5140 
                     /label="Myc-DDK"  
     misc_feature    5170..5755 
                     /label="PolyA signal"  
     misc_feature    5227..5246 
                     /label="Restriction Enzyme"  
     misc_feature    6031..6703 
                     /label="ColE1"  
     misc_feature    7133..7927 
                     /label="Kan/Neo"  
     misc_feature    7962..8264 
                     /label="SV40 ori"  
     misc_feature    8350..8827 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5031..5037 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5031 
                     /label="Pfas"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCTGTC CTTCACTTTT ATGTCCGCCC CTCTGGCCAT GAGGGGGCAG 
     1081 CATCTGGACG TGTTTTTCGG AGACTACAAG AGAAACTGCC AACACTTCAG AGTGTGGAGA 
     1141 CTGAGCTGTG CTACAATGTA CACTGGGCAG CTGAGACCCT TCCATGGGCT GAGGAGATGA 
     1201 AGAAGTTGAT GTGGCTGTTC GGCTGCCCTT TGGTGCGGGA TGATGTTGCT CAGGAGCCCT 
     1261 GGCTTGTTCC TGGCTCCAAC GATTTGTTGC TGGAGGTGGG ACCCAGGCTG AATTTCTCGA 
     1321 CTCCAGCATC GACCAACATT GTTTCTGTGT GCCAGGCAGC CGGGCTGAGG GCTGTGGATC 
     1381 GGGTAGAGAC CACCCGACGT TACCGACTTT CGTTTACCGA CCACCCTACA GCTGAAATGG 
     1441 AAGCCATCTC TCTGGCCGCC TTGCACGACC GGATGACAGA GCAGCACTAC CCTGACCCCA 
     1501 TCCAGAGCTT CTCTCCTCAG AGCATTCCAG CCCCACTCAA GGGCTCCATC GACATACTAG 
     1561 CTGAGGGTCG GCCTGCCCTG GAGAAGGCCA ACCAGGAGCT AGGTTTGGCT CTTGACTCCT 
     1621 GGGACCTCGA TTTCTATACC AAGCGCTTTC AAGAGCTGCA GCGGAACCCC AGTACAGTGG 
     1681 AGGTCTTTGA CTTGGCTCAG TCCAATAGTG AGCACAGCCG ACATTGGTTC TTCAAGGGCC 
     1741 AGCTCCATGT GGATGGGAAG AAGCTAGCGC ATTCCCTCTT TGAATCCATC ATGAGCACTC 
     1801 AGGCATCCTC CAACCCTAAC AATGTCCTCA AGTTCTGTGA CAACAGCAGT GCAATCCAGG 
     1861 GGAAGAAAGT CAAATTCCTG CGGCCGGAGG ACTCTACCCG CCCAAGCTGC TTCCAGCAAC 
     1921 AGCAGGGGCT TAGACATGTT GTCTTCACAG CAGAGACACA TAACTTCCCC ACAGGAGTGG 
     1981 CCCCCTTCAG CGGTGCAACC ACAGGCACGG GTGGCCGCAT CAGAGACGTC CAGTGCACAG 
     2041 GCCGTGGGGC CCACGTGGTA GCTGGCACTG CTGGCTATTG CTTTGGAAAC CTGCACATCC 
     2101 CAGATTATAA TCTGCCCTGG GAGGACCCAA GCTTCCAGTA TCCTGGGAAC TTTGCCAGGC 
     2161 CTTTGGAGGT TGCTATTGAG GCCAGCAATG GAGCATCTGA CTATGGCAAC AAGTTTGGAG 
     2221 AGCCAGTGCT GGCTGGATTT GCCCGCTCTT TGGGTCTCCA GCTTCCAGAT GGTCAGAGAC 
     2281 GTGAGTGGAT CAAGCCCATC ATGTTCAGTG GCGGCATTGG GTCCATGGAA GCCAAACATG 
     2341 TAGGCAAAAA GCCCCCAGAG CCAGGCATGG AGGTTGTAAA GGTTGGAGGT CCTGTTTATA 
     2401 GGATTGGAGT TGGGGGTGGA GCTGCTTCGT CTGTGCAGGT CCAGGGAGAC AACACCAGTG 
     2461 ACCTGGACTT TGGAGCTGTG CAACGGGGAG ACCCGGAAAT GGAGCAAAAG ATGAATCGTG 
     2521 TGATCCGGGC TTGTGTGGAG GCCCCAGGAG GAAACCCCAT CTGCAGCCTC CATGACCAGG 
     2581 GTGCTGGTGG CAATGGCAAT GTCCTAAAGG AGCTGAGTGA CCCAGAAGGA GCCATCATTT 
     2641 ACACCAGCCG CTTCCAGCTA GGTGACCCAA CCTTGAATGC ACTGGAAATC TGGGGGGCTG 
     2701 AGTACCAGGA GTCAAATGCA CTACTGCTTA GGCCCTCTGA CCGGGACTTC CTGAGTCGTG 
     2761 CCAGTGCCCG GGAACGCTGC CCAGCTTGTT TTGTGGGCAC AATCACTGGA GACAAGAGAA 
     2821 TTGTACTAGT GGATGATCGT GAATGCCTAG TAGGAAAAAC TGGTCAGGGA GATGCACCCC 
     2881 TGACACCTCC AACCCCTGTA GACCTGGACC TTGACTGGGT GCTGGGAAAG ATGCCTCAGA 
     2941 AGGAGTTCTT CCTCCAGAGG AAACCCCCTG TGCTGCAGCC TTTGGCTTTG CCCCCAGAAC 
     3001 TGAGTGTGCG ACAGGCTCTG AACCGGGTCC TAAGGCTGCC TGCTGTGGCC AGCAAACGCT 
     3061 ACCTCACCAA CAAGGTGGAT CGCTCTGTGG GTGGCCTTGT TGCCCAACAG CAGTGTGTCG 
     3121 GACCCCTGCA GACTCCTCTG GCTGATGTGG CTGTTGTAGC ACTGAGCCAC CAGGAGTGCA 
     3181 TAGGGGCTGC CACAGCCCTG GGAGAGCAGC CAGTCAAGAG CCTGCTGGAC CCCAAGGCTG 
     3241 CTGCCAGGCT TGCTGTGTCT GAGGCCCTCA CCAACCTGGT GTTTGCTCTG GTCACCGACC 
     3301 TCCGAGATGT GAAGTGCAGT GGGAACTGGA TGTGGGCAGC CAAGCTCCCA GGTGAGGGTG 
     3361 CAGCGCTGGC TGATGCCTGT GAGGCTATGG TGGCAGTGAT GGCAGCACTG GGTGTGGCAG 
     3421 TAGATGGTGG CAAAGACTCC CTCAGCATGG CTGCCCGGGT CGGCACTGAG ACGGTGCAGG 
     3481 CTCCTGGGTC ACTGGTCATT TCAGCATATG CTGTCTGTCC AGACATCACA GCCACTGTGA 
     3541 CCCCAGACCT CAAACATCCT GGTGGGAAAG GTCATTTGCT TTATGTGCCT TTGAGCCCCG 
     3601 GGCAGCACCG GCTGGGAGGA ACGGCTCTAG CCCAGTGTTT CTCCCAGCTT GGGGAGCACC 
     3661 CTCCTGATCT AGACCTTCCC GAGAACCTTG TGCGTGCCTT TCATATCACC CAGGGGCTGC 
     3721 TGAAAGAATG CCGTCTTTGC TCAGGCCATG ATGTCAGCGA TGGGGGGCTT GTCACCTGCC 
     3781 TTCTAGAGAT GGCCTTTGCT GGGAACTGTG GGATAGAGGT AGATGTACCT GCCCCTGGGA 
     3841 TCCATGCATT GCCTGTGCTG TTTGCGGAAG AGCCCGGTCT GGTGTTGGAG GTACAGGAGG 
     3901 CAGATGTGGC CGGAGTACGG CAGAGGTACG AGAGTGCTGG CCTACGGTGT CTGGAACTGG 
     3961 GCCACACAGG CGAGGCTGGA CCCCAGGCCA TGGCCCGAAT ATCAGTGAAT AAGGCTGTGG 
     4021 TAGTAGAGGA GCCTGTTGGG GAGTTAAGAG CCCTCTGGGA GGAAACAAGT TTCCAGCTGG 
     4081 ACCTACTGCA GGCAGAGCCA CGCTGTGTGA TAGAGGAGAA ACAGGGCCTG AAGGAGCGGA 
     4141 CAGGGCCCAG CTACTACCTG CCGCCCACCT TTCCTGTGGC CTCTGTGCCC TGTAAACCTG 
     4201 GTGGACCCGT CCCTCGAGTT GCCATCTTGC GAGAAGAGGG CAGCAATGGA GACCGAGAAA 
     4261 TGGCTGATGC CTTTCACTTG GCAGGCTTTG AGGTGTGGGA TGTGACCATG CAAGATCTCT 
     4321 GCTCTGGGGC CATCAGGTTG GACACATTCC GTGGTGTGGC CTTTGTGGGC GGCTTCAGCT 
     4381 ACGCAGACGT CCTGGGCTCT GCTAAAGGTT GGGCAGCTGC GGTGACCTTT AATCCACAGG 
     4441 CCAGGGAAGA GCTGGGCCGA TTCCGAAGGC GGCCGGACAC CTTCAGCCTG GGCGTGTGTA 
     4501 ATGGCTGTCA GCTGCTGGCT CTTCTGGGCT GGGTGGGAAG TGATCCCAGT GAAGAGCAAG 
     4561 CTGAGCCAGG CCAGGACTCC CAGCCAACAC AGCCGGGCCT CCTGCTCCGC CACAATCTGT 
     4621 CTGGGCGGTT CGAGTCCCGA TGGGCCACCG TACGTGTGGA GCCGGGGCCA GCCCTGATGC 
     4681 TCCGAGGGAT GGAAGGCTCT GTGCTGCCCG TGTGGAGTGC TCACGGGGAA GGCTACATGG 
     4741 CATTTTCTTC TCCTGAACTC CAAGCCAAGA TCGAGGCCAA GGGCCTGGTT CCTCTGCACT 
     4801 GGGCAGACGA TGATGGGAAC CCCACAGAGC AGTACCCTCT GAACCCCAAT GGATCTCCAG 
     4861 GGGGCATAGC TGGTATCTGT TCCCAAGATG GCCGACACCT GGCACTCATG CCGCACCCTG 
     4921 AGCGGGCTGT AAGGCTTTGG CAGTGGGCAT GGCGACCCTC TCCCTTTGAT GTTCTTCCCA 
     4981 CCTCCCCGTG GCTGCAGCTC TTCATCAATG CTAGGAACTG GACCCAGGAG GACAGCTGCA 
     5041 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5101 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5161 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5221 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5281 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5341 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5401 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5461 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5521 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5581 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5641 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5701 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5761 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5821 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5881 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5941 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6001 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6061 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6121 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6181 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6241 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6301 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6361 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6421 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6481 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6541 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6601 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6661 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6721 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6781 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6841 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6901 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6961 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7021 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7081 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7141 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7201 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7261 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7321 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7381 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7441 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7501 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7561 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7621 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7681 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7741 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7801 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7861 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7921 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7981 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8041 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8101 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8161 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8221 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8281 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8341 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8401 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8461 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8521 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8581 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8641 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8701 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8761 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8821 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8881 AACGCGAATT TT
//