LOCUS       MR217769                8640 bp    DNA     circular  SYN 
DEFINITION  Cyfip2 (Myc-DDK-tagged) - Mouse cytoplasmic FMR1 interacting protein 2 (Cyfip2)
ACCESSION   NM_133769
VERSION     NM_133769.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4797..4888 
                     /label="Myc-DDK"  
     misc_feature    4918..5503 
                     /label="PolyA signal"  
     misc_feature    4975..4994 
                     /label="Restriction Enzyme"  
     misc_feature    5779..6451 
                     /label="ColE1"  
     misc_feature    6881..7675 
                     /label="Kan/Neo"  
     misc_feature    7710..8012 
                     /label="SV40 ori"  
     misc_feature    8098..8575 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4779..4785 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4779 
                     /label="Cyfip2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCACCCAC GTCACTTTGG AAGATGCCCT GTCCAACGTG GACCTGCTGG 
     1081 AGGAACTGCC CCTCCCAGAC CAGCAGCCAT GTATCGAGCC CCCGCCATCC TCCATCATGT 
     1141 ACCAGGCTAA CTTTGACACG AACTTCGAGG ACAGGAATGC ATTTGTCACG GGCATTGCAA 
     1201 GGTACATTGA GCAGGCAACG GTCCACTCCA GCATGAATGA AATGCTGGAG GAGGGCCACG 
     1261 ACTATGCAGT CATGCTGTAC ACCTGGCGCA GCTGCTCCCG GGCCATCCCG CAGGTGAAGT 
     1321 GCAATGAGCA GCCCAATCGG GTCGAGATCT ACGAGAAGAC GGTGGAGGTG CTGGAGCCAG 
     1381 AGGTCACCAA GCTCATGAAG TTCATGTACT TTCAGCGCAA GGCCATCGAG CGCTTCTGTA 
     1441 GCGAGGTGAA GCGGCTGTGT CACGCTGAGC GCAGGAAGGA CTTTGTCTCT GAGGCCTACC 
     1501 TGCTGACGCT GGGCAAGTTC ATCAACATGT TCGCGGTCTT GGACGAGCTA AAGAACATGA 
     1561 AGTGCAGTGT CAAGAATGAC CACTCTGCCT ACAAGAGAGC AGCACAGTTC CTAAGGAAGA 
     1621 TGGCGGACCC CCAGTCCATC CAGGAATCTC AGAACCTGTC CATGTTCCTG GCCAATCACA 
     1681 ACAGGATCAC CCAGTGTCTT CACCAGCAAC TGGAAGTGAT CCCGGGCTAT GAGGAGCTGC 
     1741 TGGCAGACAT TGTCAACATC TGTGTTGATT ACTATGAGAA TAAGATGTAC CTGACGCCCA 
     1801 GTGAAAAGCA CATGTTGCTC AAGGTGATGG GCTTTGGTCT CTATCTGATG GACGGAAATG 
     1861 TCAGTAACAT TTACAAACTG GATGCCAAGA AGAGAATCAA CCTTAGCAAA ATTGATAAGT 
     1921 TTTTTAAGCA GCTGCAGGTG GTACCCCTTT TCGGCGACAT GCAGATAGAG CTGGCCAGAT 
     1981 ACATTAAGAC CAGTGCTCAC TATGAAGAGA ACAAGTCCAA GTGGACATGC ACTCAGAGCA 
     2041 GCATCAGCCC CCAGTACAAC ATCTGTGAGC AGATGGTTCA GATCCGGGAT GATCACATCC 
     2101 GCTTCATCTC TGAGCTGGCT CGCTACAGCA ACAGCGAGGT GGTGACAGGC TCAGGGCTGG 
     2161 ACAGCCAGAA GTCGGATGAA GAGTACCGGG AGCTTTTTGA CCTGGCCCTG CGAGGCCTGC 
     2221 AGCTTCTATC CAAGTGGAGT GCCCATGTCA TGGAGGTGTA CTCTTGGAAG CTGGTTCACC 
     2281 CCACAGACAA GTTCTGCAAC AAGGACTGCC CGGGCACCGC GGAAGAGTAC GAGAGAGCCA 
     2341 CGCGCTACAA TTATACCAGC GAGGAGAAGT TTGCCTTTGT GGAGGTGATT GCCATGATCA 
     2401 AGGGTTTGCA GGTGCTCATG GGAAGGATGG AGAGTGTCTT CAACCAGGCC ATCCGCAACA 
     2461 CTATCTACGC AGCCCTGCAG GATTTCGCCC AGGTGACGCT GCGGGAGCCG TTGCGGCAAG 
     2521 CAGTTCGAAA GAAAAAGAAT GTCCTTATCA GCGTTCTGCA AGCAATTCGA AAGACCATCT 
     2581 GTGACTGGGA GGGAGGCCGA GAGCCCCCCA ATGACCCATG CTTAAGAGGG GAGAAGGACC 
     2641 CCAAAGGTGG CTTTGACATC AAGGTGCCCC GCCGTGCTGT GGGTCCATCC AGCACACAGC 
     2701 TGTACATGGT GCGCACCATG CTTGAGTCAC TCATCGCAGA CAAGAGCGGC TCCAAGAAGA 
     2761 CGCTCAGAAG CAGCCTGGAT GGCCCTATCG TCCTTGCCAT AGAGGACTTC CACAAGCAGT 
     2821 CCTTCTTTTT CACACACCTG CTTAACATCA GCGAGGCTCT TCAGCAGTGC TGTGACCTCT 
     2881 CCCAGCTCTG GTTCCGAGAA TTCTTCCTGG AGTTGACCAT GGGCCGGCGA ATCCAGTTCC 
     2941 CGATTGAGAT GTCCATGCCC TGGATTCTAA CGGACCATAT TCTGGAAACC AAAGAACCTT 
     3001 CCATGATGGA GTATGTCCTC TACCCCTTGG ATCTGTACAA CGACAGCGCC TACTATGCCC 
     3061 TCACCAAATT CAAAAAGCAG TTCCTGTATG ACGAGATCGA AGCCGAGGTA AACCTGTGTT 
     3121 TTGATCAGTT TGTCTACAAG CTGGCTGACC AGATCTTCGC CTACTACAAA GCCATGGCCG 
     3181 GCAGTGTCCT GCTGGATAAA CGTTTTAGAG CTGAGTGTAA GAACTATGGG GTGATCATAC 
     3241 CGTACCCGCC ATCCAACCGC TACGAGACAC TGCTGAAGCA GAGACATGTC CAGCTGTTGG 
     3301 GGAGATCAAT TGACTTGAAC AGACTCATCA CCCAGCGGAT CTCTGCTGCC ATGTACAAGT 
     3361 CCTTGGACCA GGCCATCAGC CGCTTTGAGA GTGAAGACCT GACCTCCATT GTGGAGCTGG 
     3421 AGTGGCTGCT GGAGATTAAC CGACTCACAC ACCGACTCCT CTGTAAGCAC ATGACGCTAG 
     3481 ACAGCTTCGA TGCCATGTTC CGAGAAGCCA ATCATAACGT GTCTGCCCCT TACGGCCGCA 
     3541 TCACCCTGCA TGTCTTCTGG GAGCTGAACT TTGACTTTCT CCCCAACTAC TGCTATAATG 
     3601 GATCCACAAA CCGTTTTGTC CGAACTGCCA TCCCTTTCAC CCAAGAACCA CAACGGGATA 
     3661 AGCCCGCCAA TGTCCAGCCT TATTACCTCT ATGGATCCAA GCCTCTCAAC ATTGCCTACA 
     3721 GCCACATCTA TAGCTCCTAC CGGAACTTTG TGGGGCCCCC TCACTTCAAG ACCATCTGCA 
     3781 GACTGTTGGG CTACCAGGGC ATCGCTGTGG TCATGGAGGA ACTGCTGAAA ATCGTCAAGA 
     3841 GCTTGCTCCA AGGCACCATC CTGCAGTACG TGAAGACGCT CATAGAGGTG ATGCCGAAAA 
     3901 TCTGTCGTTT GCCTCGGCAC GAGTACGGCT CTCCAGGAAT CCTGGAGTTC TTCCACCACC 
     3961 AACTGAAGGA CATCATCGAG TATGCAGAAC TCAAAACAGA CGTGTTCCAG AGCCTCCGAG 
     4021 AGGTGGGCAA CGCCATCCTG TTCTGCCTCC TCATAGAGCA AGCTCTGTCT CAGGAGGAAG 
     4081 TCTGTGATTT GCTCCATGCT GCACCCTTCC AAAACATTCT GCCCCGAGTC TACATCAAAG 
     4141 AGGGGGAGCG CCTGGAAGTC CGGATGAAGC GCCTCGAGGC CAAGTACGCC CCACTTCACC 
     4201 TGGTTCCCCT GATAGAGCGG CTGGGGACGC CTCAGCAAAT CGCCATCGCT CGGGAGGGTG 
     4261 ACCTGCTGAC CAAGGAGCGG CTCTGCTGCG GCCTGTCCAT GTTTGAGGTC ATCCTGACCC 
     4321 GAATCCGGAG CTACCTGCAG GACCCCATCT GGAGGGGCCC GCCACCCACC AATGGCGTCA 
     4381 TGCATGTGGA TGAGTGTGTG GAGTTTCACC GGCTGTGGAG TGCCATGCAG TTTGTCTACT 
     4441 GTATCCCTGT GGGGACCAAC GAGTTCACAG CTGAGCAGTG CTTCGGTGAC GGCTTGAACT 
     4501 GGGCCGGCTG CTCCATCATT GTCTTGCTGG GCCAGCAGCG GCGCTTTGAC CTGTTTGACT 
     4561 TCTGTTATCA TCTGCTGAAG GTGCAGAGGC AGGACGGGAA GGATGAGATC ATTAAGAATG 
     4621 TGCCTCTGAA GAAGATGGCT GACCGCATCA GGAAGTACCA GATCTTGAAC AATGAGGTTT 
     4681 TTGCCATCCT GAACAAGTAC ATGAAGTCTG TAGAGACAGA CAGTTCCACC GTAGAGCATG 
     4741 TGCGCTGCTT CCAGCCCCCA ATCCACCAGT CATTGGCCAC CACCTGCACG CGTACGCGGC 
     4801 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4861 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4921 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4981 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5041 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5101 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5161 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5221 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5281 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5341 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5401 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5461 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5521 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5581 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5641 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5701 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5761 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5821 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5881 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5941 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6001 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6061 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6121 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6181 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6241 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6301 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6361 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6421 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6481 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6541 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6601 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6661 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6721 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6781 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6841 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6901 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6961 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7021 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7081 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7141 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7201 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7261 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7321 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7381 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7441 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7501 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7561 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7621 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7681 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7741 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7801 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7861 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7921 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7981 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8041 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8101 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8161 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8221 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8281 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8341 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8401 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8461 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8521 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8581 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//