LOCUS       MR217222                8295 bp    DNA     circular  SYN 
DEFINITION  Dusp27 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 27 (putative) (Dusp27), transcript variant 1
ACCESSION   NM_001033344
VERSION     NM_001033344.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4452..4543 
                     /label="Myc-DDK"  
     misc_feature    4573..5158 
                     /label="PolyA signal"  
     misc_feature    4630..4649 
                     /label="Restriction Enzyme"  
     misc_feature    5434..6106 
                     /label="ColE1"  
     misc_feature    6536..7330 
                     /label="Kan/Neo"  
     misc_feature    7365..7667 
                     /label="SV40 ori"  
     misc_feature    7753..8230 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4434..4440 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4434 
                     /label="Dusp27"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTACCGGT GGAGACGCAG AGGAGGAGCA GGTAGTTCCA AACGAGGAGG 
     1081 ATGAAGCAGA TGTGAGAGCT GTACAGGCCC GCTATCTCCG GAGTCCCTCC CCAAGCCAGT 
     1141 ACTCGGTGGT CTCAGAGGCA GAAACCGAAA GCATCTTCAT GGAGCCCATC CACCTCTCCT 
     1201 CGGCTGTAGC TGCCAAACAG ATCATCAACG AAGAACTCAA GCCTCGGGGG CTCAGAACAG 
     1261 ACACTGAGTG TCCAGGCATG CTGGAGTCTG CTGAACAACT GCTGGTGGAA GACCTGTACA 
     1321 ACCGTGTCCG TGAGAAGATG GATGACAGGA GCCTGTTCAA CACCCCCTGT GTGCTGGACC 
     1381 TGCAGCGGGC GCTGACCCAG GACCGGCAAG AGGCCCCTCG GAATGAGGTG GATGAGGTGT 
     1441 GGCCCAACGT CTTCATAGCT GAGAAAAGCG TGGCTGTGAA CAAGGGGCGG CTCAAGAGGC 
     1501 TGGGCATCAC CCACATTCTG AATGCCGCAC ATGGCACAGG AGTTTACACT GGTTCTGAAT 
     1561 TCTACACTGG CCTGGAGATC CAGTACCTGG GGGTGGAAGT GGATGACTTC CCAGAGGTGG 
     1621 ACATCTCCCA ACATTTCCGG AAAGCAGCTG AGTTCCTTGA CGAAGCTCTG CTGACCTACA 
     1681 GAGGGAAAGT CCTGGTCAGC AGTGAAATGG GTATCAGCAG GTCAGCAGTG CTGGTGGTCG 
     1741 CCTACCTGAT GATCTTCCAC AGCATGGCCA TCCTGGAGGC CTTGATGACT GTGCGCAGAA 
     1801 AGCGAGCTAT CTACCCCAAT GACGGCTTCC TAAAACAACT GCGGGAGCTC AATGAGAAAT 
     1861 TGATGGAGGA GAGGGAAGAG GAGGATGGTG AAGAGGAGTC TGAGGAAGAT GCCGGGAGCA 
     1921 TGCTGGGTGC TAGAGTCAAT TCACTGATGG TGGAAGAAGA AGATGATGCC ACTAGCCACC 
     1981 TGAGTGGCTC CTCCTTGGGG AAGGCCAGCC AGGTCTCCAA ACCAGTCACT CTTATTGATG 
     2041 ACGAGGAGGA GGAGAAGAAG CTGTATGAAG AGTGGAGGAA GGGGCAGGGC TTCCCCAAGG 
     2101 GGGAGGCTGC TCAGGGTAGA AAGGGACGTA GCTGTTCTAT GTCCTCAGCA CAGGATGGAG 
     2161 ACGACTGTGA GGATGAGGAT GTAGAAAGGA TAATCCAGGA GTGGCAGAGC CGAAATGAGA 
     2221 GGTACCAAGC CAAAGGGCGC GAGCAGTGGA ACCGGGAGGA AGAAGAGGAG GAAGAGAACT 
     2281 CCTACTCCAG CAGAAGGCGC AGACACACCC TAAGCGAGAG CAGTGCTTCT GAGAGTGTGA 
     2341 GCAGCCATGA CATCCGGATC CTGAAGCAGC AACTGGAGAG GAGCACCCAA AGCCGCCGCG 
     2401 GAAGATACCG CTCTGACTCG GAGTCCTCGG AGAGCACCTG GGACATGTGG AACGAGAGGC 
     2461 TGGTGGAGAT CGAGAAGGAG GCTGCCCGGA AGTACCGCTC CAAGAGCAAG AGGGAGGAGC 
     2521 TGGATGGCGA TTGTTCAGAG GCAGGGGGCA GGGTCCGAGA GGACGATGAG GAGAGCGTGT 
     2581 TGTCTGAGGC CAGCTCCTTC TACAACTTCT GCAGTAGGAA CAAGGACAAG CTCACTCCCC 
     2641 TGGAAAGGTG GAAGATTAAG AGAATCCAAT TTGGGTTTCA CAAGAAAGAC TCGGAGGCGG 
     2701 GAGACGGGGG CAGTGAGCAT GGTACAGAAG AGGCAGCAGC AGGAGAGAAG AACCTCTCTG 
     2761 ATGTCAACCT GACAGCCTAC CAGGCCTGGA AGCTGAAGCA CCAGAAAAAG GTGGGGAGTG 
     2821 AGAACAAGGA GGAAGTGGTT GAGATGAGCA AAGGAGAAGA CACGGTCTTG GCTAAGAAGA 
     2881 GACAGCGGAG ACTAGAGTTA CTGGAGAGGA GCAGGCAGAC ACTGGAGGAG AGTCAGTCCA 
     2941 TGGGAAGCTG GGAGGCAGAC AGCTCAACAG CCAGCAGGAG CATCCCCCTG TCTGCATTCT 
     3001 CGTCCGCGGC CCCTTCTGTC AGTGCGGATG GGGACACTGC GTCAGTACTC AGCACCCAGA 
     3061 GCCACCGTTC TCATGCAAGT AACATGCCCG CCACCCCCCT GCCTAACCTG CCGGTGGGGC 
     3121 CTGGAGACAC CATTTCCATT GCCAGTATCC AGAACTGGAT TGCCAATGTC GTCAATGAAA 
     3181 CCCTCGCCCA GAAGCAAAAC GAAATGCTCT TGTTGTCCCG CCCACCCTCT GTTGCAAGCA 
     3241 TGAAGGCAGC TCCAGCAGCC TGCGGCCTTG GGGGGGATGA CCAGCTCTCC GTGCTCAGCA 
     3301 CCTCCCTGAG TGGCTGCTTA CCACCTCCAA GCCAAGGGAG ACCCAGCTCC GACGTGCAGT 
     3361 CTGTGCTGTC CTCCACCAGC TCGCTGACCT CCAGGGCTGA AGGCAGTGGG AACAAAGTGA 
     3421 GGGGGACCAG CAAGCCCATC TACAGCCTCT TTGCTGACAA CGTGGACCTG AAGGAGCTTG 
     3481 GCCGGAAGGA GAAAGAGATG CAAATGGAGC TGCAGGAGAA GATGTCCGAG TATAAAATGG 
     3541 AGAAGCTGGC CTCTGATAAC AAGCGTAGCT CTCTCTTCAA GAAGAAGAAG GCCAAGGATG 
     3601 ACGAGGACAT GAGCGTGGGT GACAGAGACG AGGACACAGA CAGTGCCATC GGAAGCTTCC 
     3661 GGTACTCCTC TCGCAGTAAT TCCCAGAAGC CGGAGACAGA TGCCTCCTCT TCTCTGGCCA 
     3721 TCTCTGATCA CTATAGAAAT GGCCGCAGCA TGGGTAACGA GATGGATAGC AATATTAATA 
     3781 CGTGGCTCAG TGGCCTCAGG ATGGAAGAAA AATCTCCTCC TCAAAGTGAT TGGTCCGGAA 
     3841 GTTCCAGGGG GAGGTATACC AGGTCTTCTC TGCTCCGGGA AACAGAGTCT AAATCCTGCA 
     3901 GTTACAAGTT CTCCAAATCT AGGTCACAGG AGCAGGACAC CTCCTTCCAC GAAGCCAATG 
     3961 GTGACACTGT GCGAAACACC TCACGGTTCT CATCTTCCAC CACCAAGGAG GCCAGAGAGA 
     4021 TGCACAAGTT CTCTCGGTCC ACATTCAGCG AGACCTCGAG CTCCCGAGAG GAGAGCCCAG 
     4081 AACCCTATTT CTTCCGCCGG ACCCCTGAAC CCTCTGATGG GGAGGAGTCC CCAGAGCCAA 
     4141 GGCGTCCAAA CTGGACCAGG CCCAGGGACT GGGAAGATGT AGAAGAGTCA TCTAAATCAG 
     4201 ACTTCGCTGA GTTTGGGGCC AAGAGGAAAT TCACCCAGAG CTTCATGAGG TCTGAAGAGG 
     4261 AGGGAGAGAA GGAGAGGACA GAAAACCGAG AGGAAGGGAG GTTTGCATCT GGGCGTCAGT 
     4321 CCCAGTATCG GAGAAGCACA AACCAGCAAG AGGAAGAAGA AATGGATGAT GAAGCCATCA 
     4381 TTGCCGCCTG GAGAAAGCGG CAAGAGGAAA CCAGAACCAA ACTGCAAAGG AGGAGGGAGG 
     4441 ACACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4501 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4561 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4621 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4681 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4741 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4801 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4861 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4921 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4981 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5041 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5101 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5161 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5221 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5281 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5341 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5401 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5461 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5521 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5581 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5641 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5701 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5761 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5821 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5881 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5941 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6001 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6061 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6121 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6181 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6241 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6301 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6361 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6421 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6481 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6541 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6601 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6661 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6721 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6781 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6841 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6901 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6961 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7021 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7081 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7141 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7201 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7261 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7321 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7381 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7441 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7501 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7561 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7621 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7681 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7741 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7801 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7861 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7921 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7981 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8041 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8101 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8161 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8221 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8281 TTTAACGCGA ATTTT
//