LOCUS       MR217154                8247 bp    DNA     circular  SYN 
DEFINITION  Dsg2 (Myc-DDK-tagged) - Mouse desmoglein 2 (Dsg2)
ACCESSION   NM_007883
VERSION     NM_007883.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4404..4495 
                     /label="Myc-DDK"  
     misc_feature    4525..5110 
                     /label="PolyA signal"  
     misc_feature    4582..4601 
                     /label="Restriction Enzyme"  
     misc_feature    5386..6058 
                     /label="ColE1"  
     misc_feature    6488..7282 
                     /label="Kan/Neo"  
     misc_feature    7317..7619 
                     /label="SV40 ori"  
     misc_feature    7705..8182 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4386..4392 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4386 
                     /label="Dsg2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCGGAGC CCGGGTGACC GGTGCGCCCT GCTGCTGCTG GTGCAGCTGC 
     1081 TGGCGGTGGT CTGCTTGGAC TTTGGAAACG GACTTCACTT AGAGGTCTTC AGCCCAAGAA 
     1141 ATGAAGGCAA ACCGTTCCCT AAGCACACTC ACTTGGTTCG TCAAAAGAGG GCCTGGATCA 
     1201 CTGCCCCTGT GGCTCTGCGG GAGGGCGAAG ACCTGTCCAG AAAGAACCCG ATTGCCAAGA 
     1261 TACACTCTGA CCTTGCAGAA GAAAAAGGGA TAAAAATCAC GTACAAGTAC ACTGGGAAGG 
     1321 GAATTACAGA ACCGCCTTTC GGCATATTCG TCTTTGATAG AAACACAGGA GAACTGAACA 
     1381 TCACTAGCAT TCTTGACCGG GAAGAAACAC CATATTTTCT GCTGACAGGC TATGCATTGG 
     1441 ACTCCAGAGG AAACAACCTG GAAAAGCCCT TGGAACTACG CATCAAAGTT CTGGACATCA 
     1501 ATGACAACGA GCCAGTGTTC ACACAGGAGG TCTTTGTTGG GTCCATTGAG GAATTGAGTG 
     1561 CAGCACATAC ACTTGTGATG AAAATCACCG CCACAGATGC AGATGACCCG GAGACTCTGA 
     1621 ATGCTAAAGT CTCCTACAGA ATTGTCTCTC AGGAGCCTGC AAATAGTCAT ATGTTCTACC 
     1681 TAAATAAAGA CACGGGGGAG ATCTATACGA CCAGTTTTAC TTTGGACAGA GAGGAACACA 
     1741 GCAGCTATTC CTTGACGGTG GAAGCAAGAG ATGGTAACGG GCAGATAACA GACAAGCCAG 
     1801 TCCAGCAAGC TCAAGTTCAG ATCCGTATAT TGGATGTCAA TGACAATATA CCTGTGGTAG 
     1861 AAAACAAAAT GTATGAGGGG ACAGTGGAAG AAAACCAGGT CAATGTAGAA GTCATGCGGA 
     1921 TCAAAGTGAC CGATGCAGAT GAAGTGGGCT CTGATAACTG GCTAGCAAAC TTTACATTTG 
     1981 CATCAGGAAA TGAAGGGGGC TATTTCCACA TTGAGACTGA CACACAGACT AATGAAGGGA 
     2041 TTGTGACCCT TGTCAAGGAA GTGGACTATG AAGAAATGAA GAAGCTAGAC TTGAGCATCA 
     2101 TTGTCACTAA CAAAGCAGCT TTCCACAAGT CCATTCTGAG CAAGTACAAG GCCACGCCCA 
     2161 TTCCCATCAC TGTCAAGGTC AAGAACGTGG TTGAAGGCAT TCATTTCAAG AGCAGCGTAG 
     2221 TCTCTTTCCG AGCTAGTGAG GCAATGGATA GATCCAGCCT CAGCAGGTCG ATTGGAAATT 
     2281 TTCAAGTTTT TGATGAAGAC ACTGGTCAAG CAGCTAAAGT AACATATGTA AAAGTGCAAG 
     2341 ACACTGACAA CTGGGTCTCT GTGGACTCCG TCACTTCAGA GATTAAGCTT GTAAAGATTC 
     2401 CTGACTTTGA ATCTAGATAT GTCCAAAATG GTACCTACAC TGCAAAGGTT GTGGCCATAT 
     2461 CCAAAGAACA TCCTCAAAAA ACCATCACTG GCACCATCGT TATCACTGTT GAAGACGTCA 
     2521 ATGACAATTG TCCCGTGCTG GTGGACTCTG TACGGAGTGT CTGTGAGGAT GAACCATATG 
     2581 TGAATGTCAC TGCAGAGGAT TTGGATGGGG CCCAGAACAG TGCGCCATTC AGCTTCTCCA 
     2641 TCATTGACCA GCCTCCTGGA ACGGCACAGA AGTGGAAAAT AACGCACCAG GAAAGTACCA 
     2701 GTGTGCTGCT GCAGCAGAGC GAGCGGAAAC GCGGGAGAAG TGAGATTCCC TTCCTCATTT 
     2761 CCGACAGCCA GGGCTTCAGC TGCCCCGAAA GGCAGGTCCT TCAGCTCACT GTATGCGAGT 
     2821 GTCTGAAGGG CGGTGGCTGT GTGGCTGCAC AGTATGACAA CTACGTCGGG TTGGGCCCTG 
     2881 CCGCCATCGC TCTCATGATT CTAGCACTCC TGCTCCTGCT CCTGGTGCCG CTCTTGCTGT 
     2941 TGATATGCCA CTGTGGAGGG GGCGCCAAAG GCTTCACCCC CATTCCTGGG ACAATAGAGA 
     3001 TGCTGCACCC TTGGAATAAT GAAGGGGCAC CTCCTGAGGA CAAGGTGGTG CCATCGCTTC 
     3061 TGGTGGCCGA TCATGCAGAG AGCTCGGCAG TGAGAGGCGG CGTAGGAGGT GCGATGCTCA 
     3121 AGGAAGGCAT GATGAAAGGC AGCAGCTCAG CTTCCGTTAC CAAAGGGCAG CATGAGCTGT 
     3181 CTGAGGTTGA CGGAAGATGG GAAGAACACA GAAGCCTCCT CACCGCTGGG GCCACTCACC 
     3241 ATGTAAGGAC AGCAGGAACC ATCGCTGCCA ACGAAGCCGT AAGGACAAGA GCCACGGGGT 
     3301 CTTCCAGAGA CATGAGTGGG GCTCGAGGAG CCGTTGCCGT GAATGAGGAA TTCTTAAGAA 
     3361 GTTACTTCAC AGAGAAAGCG GCCTCCTACA ATGGGGAAGA CGACCTTCAC ATGGCCAAAG 
     3421 ACTGCCTTCT CGTTTACTCT CAGGAAGACA CGGCCTCCCT CCGAGGCTCG GTCGGGTGCT 
     3481 GCAGTTTCAT CGAGGGAGAA CTCGATGACC TGTTCCTGGA TGATCTTGGC CTTAAATTCA 
     3541 AGACCCTAGC TGAAGTTTGC CTAGGTCGAA AGATCGATCT GGATGTGGAC ATTGAACAGA 
     3601 GGCAGAAGCC GGTCAGAGAA GCGAGCGTGA GTGCAGCTTC TGGCTCGCAC TATGAGCAAG 
     3661 CGGTAACCAG CTCAGAGAGC GCGTACTCCT CTAACACCGG CTTCCCCGCC CCCAAACCTC 
     3721 TGCACGAAGT GCACACAGAG AAAGTCACAC AGGAAATCGT CACTGAGAGC TCTGTATCTT 
     3781 CCAGGCAGAG TCAGAAGGTA GTACCGCCAC CTGATCCTGT GGCTTCTGGT AATATTATAG 
     3841 TGACGGAAAC TTCCTATGCC AAAGGCTCAG CAGTGCCACC CAGCACTGTG CTCCTGGCTC 
     3901 CCAGACAGCC ACAGAGCCTG ATCGTGACAG AGAGGGTGTA TGCTCCAACC TCCACCTTGG 
     3961 TGGATCAGCA TTATGCCAAT GAAGAAAAAG TCCTTGTTAC CGAACGAGTG ATCCAGCCTA 
     4021 ATGGGGGCAT CCCTAAGCCC CTTGAGGTCA CCCAGCATCT GAAAGATGCA CAGTATGTAA 
     4081 TGGTGAGGGA AAGAGAGAGC ATCCTTGCTC CCAGCTCAGG CGTGCAGCCC ACTCTGGCAA 
     4141 TGCCCAGCGT GGCAGCAGGA GGACAGAATG TCACCGTGAC AGAAAGAATA CTAACTCCTG 
     4201 CTTCCACTCT GCAGTCCAGC TACCAGATTC CCAGTGAAAC CTCCATCACG GCTAGGAACA 
     4261 CTGTGCTCTC TAGTGTGGGA AGCATAGGTC CTCTGCCCAA TTTAGATCTA GAGGAATCTG 
     4321 ATCGTCCCAA TTCTACTATA ACCACATCTT CCACCAGGGT CACCAAGCAT AGCACCATGC 
     4381 AACATTCTTA CTCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4441 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4501 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4561 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4621 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4681 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4741 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4801 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4861 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4921 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4981 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5041 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5101 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5161 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5221 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5281 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5341 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5401 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5461 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5521 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5581 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5641 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5701 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5761 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5821 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5881 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5941 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6001 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6061 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6121 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6181 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6241 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6301 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6361 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6421 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6481 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6541 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6601 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6661 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6721 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6781 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6841 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6901 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6961 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7021 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7081 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7141 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7201 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7261 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7321 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7381 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7441 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7681 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7741 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7801 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7861 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7921 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7981 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8041 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8101 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8161 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8221 ATTTAACAAA AATTTAACGC GAATTTT
//