LOCUS       MR217126                9090 bp    DNA     circular  SYN 
DEFINITION  Plin4 (Myc-DDK-tagged) - Mouse perilipin 4 (Plin4). Note: ORF is codon optimized
ACCESSION   NM_020568
VERSION     NM_020568.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5247..5338 
                     /label="Myc-DDK"  
     misc_feature    5368..5953 
                     /label="PolyA signal"  
     misc_feature    5425..5444 
                     /label="Restriction Enzyme"  
     misc_feature    6229..6901 
                     /label="ColE1"  
     misc_feature    7331..8125 
                     /label="Kan/Neo"  
     misc_feature    8160..8462 
                     /label="SV40 ori"  
     misc_feature    8548..9025 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5229..5235 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5229 
                     /label="Plin4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGCTTCT GGCGACGGCA CGAGAGTGCC CCCAAAAAGC AAAGGCAAAA 
     1081 CACTGAGCTC TTTCTTCGGT TCCCTGCCAG GTTTTAGTAG CGCGAGGAAT CTCGTCTCCC 
     1141 ACACACACAG CAGCACTTCC ACCAAGGACC TGCAAACGGC TACAGACCCG TCAGGTACCC 
     1201 CTGCACCGAG CAGTAAAGTG TCAACAAACT CTCAGATGGC TGGAGACGCT GCCGGACTTC 
     1261 TGCAGCCGAG CGAACAGACA GCAGGAGATA AGGATATGGG ATCTTTCAGC GTCACTTCCT 
     1321 CAGAGGATGC TTTCAGCGGG GTATTTGGCA TCATGGACGC TGCCAAGGGA ATGGTACAAG 
     1381 GGGGGCTTGG AGCAACACAA TCCGCACTGG TGGGGACAAA AGAGGCCGTG AGTGGTGGAG 
     1441 TGATGGGGGC AGTGGGCGTA GCGAAGGGGC TGGTCAAAGG CGGTTTGGAT ACCTCCAAGA 
     1501 ATGTGCTCAC GAATACCAAG GACACGGTTA CTACCGGGGT AATGGGCGCA GCTAATATGG 
     1561 CCAAGGGAAC AGTTCAAACC GGCCTGGACA CCACCAAATC TGTGGTGATG GGCACAAAAG 
     1621 ACACCGTGGC TACCGGCCTG GCAGGCGCTG TGAATGTGGC TAAGGGTACC ATTCAGGGGG 
     1681 GGCTGGACAC AACCAAGTCT GTCGTGATGG GTACAAAGGA TACTGTCACC ACGGGGCTCA 
     1741 CCGGCGCTGT GAACGTGGCC AAAGGTGTGG TACAGGGCGG CCTTGACACC ACCAAATCCG 
     1801 TGGTCATGGG CACCAAGGAC ACTGTGACCA CGGGACTGAC AGGTGCAATG AATGTCGCCA 
     1861 AAGGGACTGC ACAGATGGGG ATAGATACTA GCAAAACAGT GCTCACTGGT ACAAAAGATA 
     1921 CAGTCTGTGC TGGTGCCACG GGGGCCATAA ATGTGGCTAA AGGAGCAGCC CAAGGGGGTC 
     1981 TGGACACTAC CAAGTCCGTG CTCATCGGAA CAAAGGACAC CGTGACCACC GGCTTGACAG 
     2041 GCGCTGTGAA CGTTGCTAAA GGCGCAGTGC AGGGTGGACT GGACACTACT AAGAGTGTGG 
     2101 TCATGGGGAC AAAGGACACC GTCACCACGG GACTTACCGG CGCAATGAAC GTAGCCAAAG 
     2161 GAACAGCTCA AATGGGCCTC GGGACTAGTA AGACCGTGCT GACTGGTACT AAGGACACAG 
     2221 TTTGCGCAGG GCTGACCGGC GCTATTAACG TTGCCAAAGG CGCTGCGCAA GGTGGCCTGG 
     2281 ATACCACTAA ATCTGTGTTG ATGGGCACCA AAGACACCGT TACCACAGGC CTCACCGGTG 
     2341 CTGTAAATGT GGCGAAAGGG ACCATCCAAG GCGGCTTGGA CACTACTAAA TCTGTGGTGA 
     2401 TGGGAACTAA GGACACCGTT ACCACAGGAC TCACCGGCGC CGTTAATGTG GCTAAGGGGA 
     2461 CAATTCAGGG CGGCCTCGAT ACAACCAAGT CTGTAGTGAT GGGAACTAAA GACACCGTCA 
     2521 CCACCGGATT GACTGGAGCA GTCAACGTGG CCAAGGGAGC GGCGCAGGGG GGCCTGGACA 
     2581 CCACCAAATC TGTGGTTATG GGCACCAAGG ATACCGTGAC CACTGGCCTT ACCGGCGCCA 
     2641 TGAACGTCGC CAAAGGTACC GCCCAAATGG GGCTGGGTAC CAGCAAGACT GTACTCACCG 
     2701 GAACAAAAGA CACTGTCTGC GCTGGATTGA CTGGAGCCAT TAATGTTGCC AAAGGTGCCG 
     2761 CTCAGGGAGG GCTCGACACA ACAAAGAGTG TCTTGATGGG CACCAAGGAC ACTGTCACCA 
     2821 CCGGGCTGAC CGGGGCTGTG AACGTGGCAA AGGGGACTAT ACAGGGTGGA CTGGACACCA 
     2881 CAAAGTCTGT GGTAATGGGA ACGAAGGACA CTGTGACTAC GGGGTTGACA GGGGCTGTGA 
     2941 ATGTGGCGAA GGGAGCTGTT CAGGGCGGCC TGGACACTAC CAAATCTGTC GTAATGGGCA 
     3001 CCAAGGATAC AGTGACTACT GGCTTGACCG GGGCCCTCAA TGTCGCAAAG GGAACGGCTC 
     3061 AGATGGGAAT TGATACAAGC AAAACCGTTT TGATCGGGAC CAAGGACACA GTCTGCGCGG 
     3121 GAGCGACGGG TGCCATCAAC ATGGCAAAGG GGGCTGCCCA GGGTGGCCTC GATACCACCA 
     3181 AATCCGTCCT CATGGGCACA AAGGATACAG TTACAACTGG CCTGACAGGC GCTATCAACG 
     3241 TGGCCAAAGG CTCCGCTCAG GGCGGTCTGG ACACGACCAA AAGTGTCCTG ATCGGAACGA 
     3301 AGGATACTGT TACCACTGGG CTCACCGGGG CCCTCAATGT TGCGAAGGGA ACCGTGCAAA 
     3361 CTGGACTGGA CACGAGCCAG AGGGTATTGA CAGGCACAAA GGACAACGTG TATGCCGGCG 
     3421 TGACTGGAGC CGTCAACGTA GCCAAGGGTA CAATCCAGGG AGGCCTTGAC ACCACAAAGA 
     3481 GCGTGGTGAT GGGCACCAAG GATACAGTTA CAACTGGCCT CACCGGTGCT GTCAATGTGG 
     3541 CAAAAGGCGC CGTTCAAGGA GGTCTGGACA CCACTAAGTC TGTGGTGATG GGCACAAAGG 
     3601 ATACTGTTAC CACCGGTTTG ACCGGGGCAA TGAATGTGGC TAAGGGGACT GCCCAGATGG 
     3661 GAATTGACAC AAGTAAAACC GTACTGACCG GGACTAAAGA TACAGTTTGC GCTGGTCTGA 
     3721 CGGGCGCTAT CAATGTTGCC AAGGGAGCTA CTCAGGGTGG CCTGGACACC ACCAAGTCCG 
     3781 TACTGATGGG CACCAAAGAT ACTGTTACTA CCGGCCTTAC GGGTGCAATC AATGTCGCAA 
     3841 AGGGAGCTGC TCAGGGTGGT TTGGACACAA CTAAAAGTGT CCTGCTCGGA ACCAAGGATA 
     3901 CCGTAACCAC GGGGCTGACG GGCGCCGCGA ATGTTGCTAA AGAAACGGTG CAGATGGGCC 
     3961 TCGATACAAG CAAGAACATA CTTATGGATA CAAAGGATTC CATTTGCGCA GGTGCCACCG 
     4021 GAGCCATTAC GGTGGTCAAG GGCGCAGCCC AAGGCGGTCT TGATACTAGC AACGCAGCCC 
     4081 TGACCGGCAC TATGGACACC GCGAAAGGGA CCGTGCAAAC CTCTCTCGAC ACTTCCAAAC 
     4141 ACATGTTGAT TGGCATGAAG GACACAGTCT GCGCTGGGGT CACATCTGCA ATGAATATGG 
     4201 CCAAAGGGAT CCATAAGAAT ACAGATACAA CTAGGGACAC CCAGTCAAGC GTTCTTGCCC 
     4261 ACTCCGGAAA CGTTGCTACT AACGCCATCC ACACAGGGGT GCATACCGTC CCATCTTCCC 
     4321 TTTCCGGAAG TCACTCTATC ATTTGTCACG AACCGTCTAT TTACCGAGCC ACAAACCACG 
     4381 GCGTTGGACA AGCAATTCTC ACTAGTACCG AGAGCCTGTG CTGTGAAACA TCATCATTCA 
     4441 GTGACAAATA CGGACTGGGG CATGTGACTG AACCCCGGGC TGATACCAAG ACGCTGGTGT 
     4501 CAGGCATGGC TTCATCTGCC TGCGCGGCCA CACGGAGCGT GGAGGAGTGC GGCCAATTGG 
     4561 CCGCCACTGG CTTTGCTGCT CTCCCAGATG AATTGAAGGG GCTCGGTGAC ATCTTCCAGC 
     4621 CCATGACCAC AGAAGAGCAG GCTCAGCTCG CTGTCTCTGA ATCTGGCCCC CGAGTACTTT 
     4681 CTGCCGACCG GGGCTCCTAT TATATACGGT TGGGCGACCT TGCTCCGTCC TTCAGGCAAA 
     4741 GAGCCTTCGA ACATGCACTG TCTCATATAC AACATAACCA GTTCCAAGCC AGGGCCGCAC 
     4801 TGGCTCAGCT CCAGGAAGCT TTCCAGATGA CTGATATGAC AATGGAAGCC GCATGTGGCA 
     4861 AGCTGTGTTC CGACCAGAGC CTGAATACTA TGGTGGAGGC TGTGGGTAGC CACGAGATGA 
     4921 GAGCTAGTGT GGCCCAGGAC AGACTTTGCA CGCTCGCCCA TCAGCTGCAC GCTGCTTATT 
     4981 CATCCCTCGT CACCAGTCTG CAGGGGCTTC CCGAAGTGCA ACAACAAGCC GGTCAGGCCC 
     5041 GGCACAGCCT TTGCAAATTG TACGGCCTCG TGTCTTCCGA GGCAGGTTCT GAACTGCAGA 
     5101 CGGAGCAGCT TGCCCAGAGT AGCGCCGGCG TGGTTGAAGC ATGGCAGGGC CTGGAGGTTT 
     5161 TGCTTGAGAA GCTTCAGCAG AACCCACCTC TCAGCTGGTT GGTAGGGCCT TTCACTTCTA 
     5221 TGCCATGTGG TCAACTCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5281 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5341 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5401 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5461 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5521 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5581 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5641 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5701 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5761 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5821 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5881 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5941 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6001 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6061 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6121 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6181 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6241 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6301 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6361 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6421 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6481 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6541 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6601 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6661 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6721 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6781 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6841 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6901 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6961 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7021 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7081 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7141 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7201 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7261 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7321 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7381 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7441 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7501 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7561 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7621 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7681 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7741 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7801 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7861 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7921 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7981 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8041 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8101 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8161 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8221 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8281 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8341 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8401 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8461 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8521 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8581 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8641 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8701 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8761 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8821 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8881 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8941 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9001 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9061 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//