LOCUS       MR216588L3                8972 bp    DNA     circular  SYN 
DEFINITION  Kcnc2 (Myc-DDK-tagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 2 (Kcnc2)
ACCESSION   NM_001025581
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7263..7443 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8171..8830 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7491..8110 
                     /label="ColE1"  
     misc_feature    5303..5326 
                     /label="DDK"  
     misc_feature    6138..6158 
                     /label="LR50"  
     misc_feature    5255..5326 
                     /label="MYC"  
     misc_feature    5336..5401 
                     /label="P2A"  
     misc_feature    5402..6001 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    6147..6738 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5237..5243 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5237 
                     /label="Kcnc2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGCAAGATC GAGAGCAACG AGAGGGTGAT 
     3361 CCTCAATGTC GGGGGTACCA GGCACGAAAC CTACCGCAGC ACCCTCAAGA CCCTGCCTGG 
     3421 AACTCGCCTG GCCCTTCTTG CCTCCTCTGA ACCTCAGGGC GACTGCCTGA CTGCGGCCGG 
     3481 GGACAAGCTG CAACCGCTGC CCCCTCCGCT GTCTCCGCCG CCACGACCGC CTCCCTTGTC 
     3541 CCCTGTCCCC AGCGGCTGCT TCGAGGGCGG CGCAGGCAAC TGCAGTTCGC ACGGTGGCAA 
     3601 CGGCGGCAAC GGCGGCAGCG ACCACCCTGG GGGAGGCCGC GAATTCTTCT TCGATCGCCA 
     3661 CCCAGGAGTA TTCGCCTATG TGCTCAATTA CTACCGCACG GGCAAGCTGC ACTGCCCCGC 
     3721 CGACGTGTGC GGGCCGCTCT TCGAGGAAGA GCTGGCTTTC TGGGGCATCG ATGAGACCGA 
     3781 CGTGGAGCCC TGCTGCTGGA TGACCTACAG GCAGCACCGG GACGCGGAGG AGGCCCTGGA 
     3841 CATCTTTGAG ACACCCGACC TCATCGGGGG CGACCCTGGT GATGATGAGG ACCTAGCGGC 
     3901 CAAGAGATTG GGCATTGAGG ATGCTGCGGG GCTGGGAGGA CCCGATGGCA AGTCAGGCCG 
     3961 CTGGAGGAAG CTGCAGCCTC GCATGTGGGC TCTTTTTGAG GACCCCTACT CATCTAGAGC 
     4021 CGCTAGGTTT ATTGCTTTTG CTTCTTTGTT CTTCATTTTG GTTTCCATCA CAACCTTTTG 
     4081 CCTGGAGACA CATGAAGCTT TCAATATTGT TAAAAACAAG ACGGAGCCCG TCATCAATGG 
     4141 CACCAGCCCG GTCCTCCAGT ACGAAATCGA AACGGATCCC GCCCTGACGT ACGTGGAAGG 
     4201 AGTATGTGTG GTGTGGTTTA CGTTTGAATT TTTAGTCCGA ATTGTTTTCT CACCCAATAA 
     4261 ACTTGAATTC ATCAAAAATC TCTTGAACAT CATTGACTTT GTGGCCATCC TCCCCTTCTA 
     4321 CCTAGAGGTG GGACTCAGCG GGCTGTCCTC CAAAGCGGCC AAAGATGTGC TCGGCTTTCT 
     4381 CAGGGTGGTT AGGTTTGTGA GGATCCTGAG AATCTTCAAG CTCACCCGCC ATTTCGTAGG 
     4441 TCTGAGGGTG CTCGGACATA CTCTTCGGGC GAGCACCAAC GAATTTTTGT TGCTGATCAT 
     4501 CTTCCTGGCG CTGGGAGTTT TGATATTCGC TACGATGATC TACTACGCTG AGAGAGTAGG 
     4561 GGCTCAGCCC AATGACCCTT CAGCTAGTGA GCACACGCAG TTCAAAAACA TCCCCATTGG 
     4621 TTTCTGGTGG GCCGTAGTGA CCATGACTAC CTTAGGTTAC GGGGATATGT ACCCCCAAAC 
     4681 ATGGTCAGGG ATGTTGGTGG GGGCCTTGTG TGCCCTGGCC GGAGTGCTGA CAATAGCCAT 
     4741 GCCTGTGCCT GTCATTGTCA ACAATTTTGG AATGTACTAC TCCTTGGCAA TGGCGAAGCA 
     4801 GAAACTTCCA AGAAAGAGAA AGAAGCATAT TCCTCCTGCC CCTCTGGCAA GCTCGCCTAC 
     4861 ATTTTGCAAG ACAGAATTGA ACATGGCTTG CAACAGTACC CAGAGTGACA CATGTCTGGG 
     4921 CAAAGAAAAC CGGCTTCTGG AACATAACAG ATCAGTGTTA TCAGGTGACG ACAGTACAGG 
     4981 AAGTGAGCCG CCATTATCAC CTCCAGAAAG GCTCCCTATC AGACGCTCTA GTACCAGAGA 
     5041 CAAAAACAGA AGAGGGGAAA CGTGTTTCCT GTTGACGACA GGTGATTACA CGTGCGCTTC 
     5101 TGATGGAGGA ATCAGGAAAG CAAGCACACT AGAGCCCATG GAGAGTACTG CACAGACTAA 
     5161 AGGAGACACA AGACCAGAAG CTCATTGGAA TTGTGCGCAC TTACTCAATT TTGGGTGTCC 
     5221 TACAGGAAGT TCATTTCCCA CCCTCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC 
     5281 AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTG 
     5341 GGTAGGAAGC GGAGCTACTA ACTTCAGCCT GCTGAAGCAG GCTGGAGACG TGGAGGAGAA 
     5401 CCCTGGACCT ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC 
     5461 CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT 
     5521 CGATCCGGAT CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT 
     5581 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC 
     5641 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA 
     5701 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG 
     5761 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA 
     5821 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC 
     5881 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC 
     5941 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC 
     6001 CGGTGCCTGA TGTACACCCA AACGGCCGGC CGCGGTCTGT ACAAGTAGGA TTCGTCGAGG 
     6061 GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ACATGTTTAA GGGTTCCGGT 
     6121 TCCACTAGGT ACAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT 
     6181 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 
     6241 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT 
     6301 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG 
     6361 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC 
     6421 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG 
     6481 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT 
     6541 TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC 
     6601 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG 
     6661 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA 
     6721 TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGTCGACC TCGATCGAGA CCTAGAAAAA 
     6781 CATGGAGCAA TCACAAGTAG CAATACAGCA GCTACCAATG CTGATTGTGC CTGGCTAGAA 
     6841 GCACAAGAGG AGGAGGAGGT GGGTTTTCCA GTCACACCTC AGGTACCTTT AAGACCAATG 
     6901 ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG 
     6961 CTAATTCACT CCCAACGAAG ACAAGATATC CTTGATCTGT GGATCTACCA CACACAAGGC 
     7021 TACTTCCCTG ATTGGCAGAA CTACACACCA GGGCCAGGGA TCAGATATCC ACTGACCTTT 
     7081 GGATGGTGCT ACAAGCTAGT ACCAGTTGAG CAAGAGAAGG TAGAAGAAGC CAATGAAGGA 
     7141 GAGAACACCC GCTTGTTACA CCCTGTGAGC CTGCATGGGA TGGATGACCC GGAGAGAGAA 
     7201 GTATTAGAGT GGAGGTTTGA CAGCCGCCTA GCATTTCATC ACATGGCCCG AGAGCTGCAT 
     7261 CCGGACTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA 
     7321 CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT 
     7381 GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG 
     7441 AAAATCTCTA GCAGCATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC 
     7501 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC 
     7561 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA 
     7621 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT 
     7681 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG 
     7741 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC 
     7801 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG 
     7861 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 
     7921 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG 
     7981 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC 
     8041 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT 
     8101 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 
     8161 TAAGGGATTT TGGTCATGAT TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT 
     8221 CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC ATGATGAACC TGAATCGCCA 
     8281 GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA 
     8341 AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG 
     8401 CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT 
     8461 AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC 
     8521 TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC 
     8581 TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG GAACTCCGGA TGAGCATTCA 
     8641 TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG 
     8701 TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG 
     8761 ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC 
     8821 CAGTGATTTT TTTCTCCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8881 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8941 CGCGCACATT TCCCCGAAAA GTGCCACCTG AC
//