LOCUS       MR216210                7299 bp    DNA     circular  SYN 
DEFINITION  Adam1b (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 1b (Adam1b)
ACCESSION   NM_172125
VERSION     NM_172125.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3456..3547 
                     /label="Myc-DDK"  
     misc_feature    3577..4162 
                     /label="PolyA signal"  
     misc_feature    3634..3653 
                     /label="Restriction Enzyme"  
     misc_feature    4438..5110 
                     /label="ColE1"  
     misc_feature    5540..6334 
                     /label="Kan/Neo"  
     misc_feature    6369..6671 
                     /label="SV40 ori"  
     misc_feature    6757..7234 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3438..3444 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3438 
                     /label="Adam1b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGACTA AAGCTGGGGA AAATCCCAGA GCACTGGTGT ATCAGGCTGG 
     1081 TGGCCATGTT GCTCTTGGCA ATAATTTTTC TCCCGAGCAC GTTCTGTGAC ATTGGATCTG 
     1141 TATATAATTC TTCCTATGAA ACTGTCATCC CTGAGAGACT GCCAGGCAAG GGGGGGAAAG 
     1201 ACCCTGGAGG GAAGGTGTCC TACATGCTAT TGATGCAAGG CCAAAAGCAG CTGCTTCACC 
     1261 TCGAGGTAAA GGGACACTAC CCTGAGAATA ACTTCCCAGT CTACAGTTAC CACAATGGCA 
     1321 TCCTGAGGCA AGAAATGCCT CTCCTCTCCC AGGACTGCCA CTATGAAGGC TACATGGAAG 
     1381 GGGTGCCAGG CTCCTTTGTT TCTGTCAACA TCTGTTCAGG CCTCAGGGGG GTCTTGATTA 
     1441 AAGAGGAAAC ATCCTATGGC ATTGAGCCCA TGCTCTCTTC CAAAAACTTT GAACATGTCC 
     1501 TCTACACCAT GGAGCATCAG CCTGTGGTCT CCTGCAGTGT CACTCCCAAA GACAGCCCTG 
     1561 GGGACACCAG CCATCCACCA AGGAGCAGGA AGCCCGATGA CCTACTGGTT CTGACTGACT 
     1621 GGTGGTCACA CACCAAGTAT GTGGAGATGT TTGTGGTGGT CAACCACCAG CGGTTCCAGA 
     1681 TGTGGGGCAG TAACATCAAC GAGACGGTCC AGGCAGTAAT GGACATCATT GCTCTGGCCA 
     1741 ACAGCTTCAC TAGGGGGATA AACACAGAGG TGGTGCTGGT GGGCCTGGAA ATCTGGACAG 
     1801 AGGGGGACCC GATAGAGGTC CCAGTGGACC TGCAGACCAC ACTCAGGAAT TTCAACTTCT 
     1861 GGAGACAGGA GAAACTCGTG GGCCGGGTCA GGCACGATGT GGCACACTTG ATCGTCGGGC 
     1921 ATCGCCCAGG AGAGAACGAG GGCCAGGCGT TTCTCCGTGG TGCCTGTTCG GGTGAGTTTG 
     1981 CGGCGGCCGT GGAGGCCTTC CATCATGAAG ATGTCCTCCT GTTCGCGGCT CTCATGGCCC 
     2041 ACGAGCTCGG GCACAACCTG GGTATCCAGC ACGACCACCC GACCTGCACC TGTGGTCCCA 
     2101 AGCACTTCTG CCTCATGGGT GAGAAGATCG GTAAGGACAG TGGCTTCAGC AACTGCAGCT 
     2161 CTGACCACTT CCTCCGTTTC CTCCACGACC ACAGAGGGGT GTGCCTGCTT GATGAGCCTG 
     2221 GGCGCCAGAG CCGCATGCGC AGAGCTGCCA ATTGTGGGAA TGGTGTGGTG GAGGACTTGG 
     2281 AGCAGTGTGA CTGTGGCAGT GACTGTGATA AAAGCCAGTG TTGTGATGAA AATTGTAAAC 
     2341 TGAAGGGCAA CTCAGTGTGC AGCACTGAAC TTTGCTGTTT TAAATGTAAC TTTAAAAAGG 
     2401 AAGGAGATGT TTGCCGTCCT GCTGACGGAC CATGTGACCT GGAAGAATAC TGCAATGGGA 
     2461 CCTCTGCAGC ATGTCCCAGT GACAGGAAAG CCCAGGACGG CTCTAAGTGC CACGAATCCT 
     2521 TCTTGTGCTT TAATGGTCAG TGCATGGACC CTACCTTTCA GTGCTCTCGT ATTTTTGGGC 
     2581 ATGGTTCAAG GTCTGCCTCC GATTATTGCT ACACCTCTCT GAACTCGAGA GGAGACCAGT 
     2641 TTGGAAACTG TGGCTCCTCC TCTCAATTTC CAAAAAAGTA TACCAAGTGT TCAGATAAAA 
     2701 ATGTAATGTG TGGGAAACTT ATATGTACAG AGGTGGCCTT CCTGCCACAA ATCCAACCGA 
     2761 ACAACTTGCT GCTCCAGGTC CCTGAGACCG AGGACTGGTG CTGGAGTGTA GCTGTGTTTG 
     2821 ACATGAGAGA TTCCCTCCAT GAGGAATACG TGAAGGACAA CACTTACTGT GGCAAAGATA 
     2881 AAGTCTGTAA AAATTCCATT TGTGAGGACT TCACTCCATT CTCATTTCCC TGTAGTCCAA 
     2941 GCAAACAATG CAACAAGCAC GGGGTGTGCA ATGACCTGGG GAACTGCCAC TGCTCCTTCG 
     3001 GCTTTGCACC TCCCGACTGC AAAGAGGAAG GCACTGGGGG CAGTGTGGAC AGTGGCCCTG 
     3061 CTGTTAATCT GTCAAATGAC AGCAGTCCAG GGCCGAACTC CACCCAATCC AGCACAGAGG 
     3121 AGCTGATCCT TAACTTAAAG CTGATCGTCC TGGCTGTCAT TCTGGTGCTC ATGATACTCT 
     3181 TAATAATCAT ATGCATCATA AGTGCCTACA CCAAGTCAGA GACCGCTTCC GAAGCAGGGC 
     3241 CTTCAGAGCT AGAGGAGCTT CCAGAAGGGG AGAAGGAAGA GCAGGAGGAG GTCCTACCAG 
     3301 AAGAGGCTAA AGGGGAGGAG GAGGAGTTGG AGTATGGGAA GGAAGAAGCA GAAGAGCAAG 
     3361 GAGCAGTAGA AGAGGAAGGA GCAGAAGAGG CAAACGAAGA AGCAGCAGCA GAAAAGAAAG 
     3421 ACGAAGATGA AGAAGAGGGA GAGGAAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3481 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3541 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3601 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3661 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3721 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3781 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3841 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3901 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3961 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4021 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4081 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4141 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4201 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4261 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4321 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4381 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4441 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4501 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4561 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4621 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4681 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4741 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4801 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4861 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4921 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4981 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5041 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5101 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5161 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5221 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5281 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5341 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5401 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5461 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5521 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5581 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5641 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5701 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5761 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5821 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5881 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5941 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6001 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6061 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6121 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6181 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6241 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6301 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6361 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6421 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6481 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6541 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6601 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6661 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6721 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6781 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6841 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6901 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6961 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7021 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7081 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7141 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7201 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7261 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//