LOCUS       MR216112                7845 bp    DNA     circular  SYN 
DEFINITION  Eml4 (Myc-DDK-tagged) - Mouse echinoderm microtubule associated protein like 4 (Eml4), transcript variant 1
ACCESSION   NM_001114361
VERSION     NM_001114361.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4002..4093 
                     /label="Myc-DDK"  
     misc_feature    4123..4708 
                     /label="PolyA signal"  
     misc_feature    4180..4199 
                     /label="Restriction Enzyme"  
     misc_feature    4984..5656 
                     /label="ColE1"  
     misc_feature    6086..6880 
                     /label="Kan/Neo"  
     misc_feature    6915..7217 
                     /label="SV40 ori"  
     misc_feature    7303..7780 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3984..3990 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3984 
                     /label="Eml4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGGTTTC GCCGGCAGCC TCGATGATAG TATTTCTGCT GCAAGTACCT 
     1081 CTGACGTTCA AGACCGCCTC TCAGCTCTTG AGTCACGAGT TCAACAACAA GAAGATGAAA 
     1141 TCACTGTGCT AAAGGCAGCA TTGGCTGATG TTTTGAGGCG TCTTGCAATC TCTGAAGATC 
     1201 ATGTAGCCTC AGTGAAAAAG TCCATGCCAA GTAAAGGCCA ACCAAGCCTT CGAGAAGCTA 
     1261 TTTCTATGTC CTGTATAACC AATGGAAGTG GTATAAGCAG AAAACAGAAT CATACCAGCT 
     1321 CTGTATCAAT AGCAAGAAAA GAAACTCTTT CTTCAGCTGC TAAAAGTGGT ACAGAAAAAA 
     1381 AGAAAGAAAA GCCACAAGGA CAGAGAGAAA AAAAAGAGGA TTCTCATTCT AATGATCAGA 
     1441 GCCCACAAAT TCGAGCGTCA CCTTCTCCCC AGCCCTCTTC ACAGCCTCTC CAAATAAACA 
     1501 GACAAACTCC AGAGAGCAAG AGTTCTGCTC CCATCAAAAG CATAAAACGT CCACCAACAG 
     1561 CTGAAAAGTC ACATAACTCA TGGGAAAATT CAGATGATAG TCGTAATAAA TTAATGAAAA 
     1621 CGGTTTCAAC ATCAAAACTA ATATCAAAGG TTATAAAAAA TGCAGACAAA CATAAAGATG 
     1681 TCATCGTCAA TCAAGCAAAA ATGTCAACCC GGGAAAAAAA CAGCCAAGAA GGAGAATATA 
     1741 TTAAAATGTT TATGCGAGGT CGGCCAATTA CAATGTTCAT TCCTTCGGAT GTTGACAACT 
     1801 ATGATGACAT CAGAACAGAA CTGCCTCCAG AGAAGCTCAA ACTGGAGTGG GTATACGGTT 
     1861 ATCGAGGAAA GGACTGTAGA GCGAATGTAT ACCTGCTTCC CACAGGGGAG ATAGTTTATT 
     1921 TTATCGCCTC AGTGGTAGTC CTGTTTAATT ATGAGGAGAG GACTCAGCGA CACTACCTGG 
     1981 GTCATACAGA CTGTGTGAGA TGCCTTGCTG TACATCCTGA CAAGATTAGA ATTGCAACTG 
     2041 GACAGATAGC CGGAGTAGAC AAAGATGGAA GGCCTCTGCA ACCCCATGTC AGGGTGTGGG 
     2101 ATTCAGTAAG TCTTACCACA CTGCATGTTA TTGGACTTGG AACTTTTGAG CGTGGAGTAG 
     2161 GATGCCTGGA TTTTTCAAAA GCAGATTCAG GTGTTCATTT ATGCGTTATT GATGACTCCA 
     2221 ATGAGCACAT GCTTACTGTA TGGGACTGGC AGAAAAAATC AAAAATCGCA GAAATAAAGA 
     2281 CAACAAACGA AGTTGTTTTG GCTGTAGAAT TCCACCCAAC AGATGCAAAT ACAATAATAA 
     2341 CATGTGGTAA ATCTCATATT TTTTTCTGGA CCTGGAGTGG CAACTCACTA ACAAGAAAAC 
     2401 AGGGAATTTT TGGGAAATAT GAAAAACCAA AATTCGTTCA GTGTTTGGCA TTCTTGGGGA 
     2461 ATGGAGATGT TCTCACTGGA GACTCGGGTG GAGTCATGCT GATCTGGAGC AAAACGATGG 
     2521 TAGAGCCCCC GCCCGGGAAA GGACCTAAAG GTGTGTATCA GATCAACAGA CAAATCAAAG 
     2581 CTCATGATGG CAGTGTGTTT ACACTTTGTC AGATGAGAAA TGGGATGCTA TTAACTGGAG 
     2641 GCGGAAAAGA CAGAAAAATA ATTTTGTGGG ATCATGATCT GAACCTTGAA AGAGAAATAG 
     2701 AGGTTCCTGA TCAGTATGGC ACCATTAGAG CAGTTGCAGA GGGAAGGGCA GAACAGTTCT 
     2761 TAGTAGGCAC ATCACGAAAC TTTATTTTAC GGGGAACGTT TAATGATGGC TTCCAAATAG 
     2821 AAGTCCAGGG TCATACAGAT GAACTCTGGG GCCTTGCAAC ACATCCCTTC AAAGATTTGC 
     2881 TCTTGACGTG TGCTCAGGAC AGGCAGGTGT GCATGTGGAA CTCAGTGGAG CACAGGCTGG 
     2941 AGTGGACCAG GCTTGTGGAC GAACCAGGAC ACTGTGCAGA CTTTCATCCA AGCGGTACAG 
     3001 TGGTAGCCAT TGGAACGCAC TCAGGCAGGT GGTTTGTTCT GGATGCAGAA ACAAGAGATC 
     3061 TGGTGTCAAT CCACACGGAT GGCAATGAGC AGCTGTCTGT GATGCGCTAC TCAGTAGATG 
     3121 GCACTCTTCT GGCTGTGGGC TCTCATGACA ACTTTATTTA CCTCTACACA GTCTTAGAAA 
     3181 ACGGAAGAAA ATACAGCAGA TATGGAAAGT GCACTGGACA TTCTAGCTAC ATCACACACC 
     3241 TTGACTGGTC CCCAGACAAT AAGCATATAA TGTCTAACTC GGGTGACTAC GAGATACTGT 
     3301 ACTGGGACAT TGAAAATGGC TGCAAACTAA TCAGGAACCG ATCAGATTGC AAGGACATCG 
     3361 ATTGGACAAC ATATACCTGT GTGCTAGGCT TCCAAGTCTT TGGGGTGTGG CCGGAAGGGT 
     3421 CCGATGGAAC AGACATCAAC GCACTGGTTC GATCCCACAA CAGAAGAGTG ATCGCAGTGG 
     3481 CCGATGATTT CTGCAAAGTT CATCTCTTTC AGTACCCCTG CTCCAAAGCC AAGGCTCCCA 
     3541 GTCACAAGTA CAGTGCCCAC AGCAGCCATG TGACCAACGT CAGTTTTACT CATAATGACA 
     3601 GTCACCTAAT TTCAACTGGT GGGAAGGATA TGAGCATCAT TCAGTGGAAG CTTGTGGAAA 
     3661 AGTTACCTGT GCCTCAAAAC GAGGTCATAA CAGACGCCAG TGTGACCAAA ACCCCTGCCT 
     3721 CTTCCTCTGA AACTGCCAGG CCGTCTAACT CTCCTCCACT GCCTCCTTCT CTACCGTTAA 
     3781 CTGGGACGGC TGAAGAGGAA AGCAGGATGG GCAGCTCCCC CACACTAGTG GAGAACAGCC 
     3841 TGGAGCAGAT CGCAGAGCCC AGCGAGGAGC AGAGCGAGTG GGGTAGCGAA GACCTTGGTG 
     3901 TGGTGATTGA TGAAGAGCCT GCTAGTGAGT TAAGTGAGAC ACAGGGAGCA ACTGAGCTGC 
     3961 CTGAGGAGGA GCGAGGCATA ACACCCCTGT GCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4021 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4081 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4141 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4201 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4261 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4321 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4381 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4441 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4501 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4561 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4621 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4681 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4741 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4801 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4861 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4921 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4981 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5041 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5101 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5161 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5221 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5281 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5341 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5401 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5461 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5521 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5581 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5641 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5701 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5761 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5821 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5881 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5941 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6001 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6061 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6121 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6181 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6241 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6301 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6361 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6421 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6481 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6541 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6601 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6661 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6721 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6781 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6841 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6901 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6961 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7021 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7081 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7141 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7201 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7261 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7321 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7381 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7441 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7501 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7561 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7621 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7681 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7741 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7801 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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