LOCUS       MR215850                9423 bp    DNA     circular  SYN 
DEFINITION  Asxl1 (Myc-DDK-tagged) - Mouse additional sex combs like 1 (Drosophila) (Asxl1)
ACCESSION   NM_001039939
VERSION     NM_001039939.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5580..5671 
                     /label="Myc-DDK"  
     misc_feature    5701..6286 
                     /label="PolyA signal"  
     misc_feature    5758..5777 
                     /label="Restriction Enzyme"  
     misc_feature    6562..7234 
                     /label="ColE1"  
     misc_feature    7664..8458 
                     /label="Kan/Neo"  
     misc_feature    8493..8795 
                     /label="SV40 ori"  
     misc_feature    8881..9358 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5562..5568 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5562 
                     /label="Asxl1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGACAAA CAGAAGAGGA AGAAGGAGCG CACGTGGGCC GAGGCCGCGC 
     1081 GCCTGGTGTT AGAAAACTAC TCAGATGCTC CAATGACACC AAAACAGATT CTGCAGGTCA 
     1141 TAGAGGCAGA AGGACTGAAG GAAATGAGAA GTGGGACATC CCCTCTTGCG TGCCTCAATG 
     1201 CCATGCTACA TTCCAACTCA AGAGGAGGAG AAGGGCTGTT TTATAAATTA CCTGGCCGCA 
     1261 TTAGTCTTTT CACACTCAAG AAAGATGCAG TGCAGTGGTC TAGAAATGCA GCTACAGTGG 
     1321 ATGGAGACGA GCCAGAGGAC TCCGCTGATG TGGAAAGCTG TGGGTCTAAT GAAGCCAGCA 
     1381 CTGTGAGTGG TGAAAATGAT GTATCTCTGG ATGAAACATC TTCAAATGCA TCCTGCTCTA 
     1441 CAGAGTCTCA GAGCCGACCC CTCTCCAATC CCAGGGACAG CCACAGGGCT TCCTCACAGG 
     1501 CAAACAAACA GAAGAAAAGG ACTGGGGTTA TGCTACCTCG TGTTGTCCTG ACTCCTCTGA 
     1561 AGGTAAACGG GGCCCACGTG GAACCTGCGT CAGGATTCTC AGGCCGCCAC GCAGATGGCG 
     1621 AGAGTGGCAG TCCATCGAGC AGCAGCAGCG GTTCTCTGGC CTTGGGCAAC AGTGCCATTC 
     1681 GAGGCCAGGC CGAGGTCACT CGGGACCCTG CCCCCCTCTT AAGAGGCTTC CGGAAGCCAG 
     1741 CCACAGGGCA AATGAAGCGC AACAGAGGGG AAGAGGTAGA TTTTGAGACG CCTGGGTCCA 
     1801 TTCTTGTTAA CACCAACCTC CGTGCTCTGA TAAACTCTCG GACCTTCCAT GCCCTGCCAC 
     1861 TACACTTCCA GCAGCAACTC CTCCTCCTCC TGCCTGAAGT GGACAGACAG GTGGGGACAG 
     1921 ATGGCCTGCT GCGCCTCAGC GGCAGTGCAC TCAATAATGA GTTTTTCACC CATGCAGCTC 
     1981 AGAGCTGGCG AGAACGCCTT GCTGATGGTG AATTCACTCA TGAGATGCAA GTCAGGCTAA 
     2041 GACAGGAAAT GGAAAAGGAG AAGAAGGTGG AACAATGGAA GGAAAAGTTC TTTGAAGATT 
     2101 ACTACGGACA GAAATTGGGT TTGACCAAAG AAGAATCACT GCAGCAGAAA GAGGTCCAGG 
     2161 AGGAGGCCAA AGTCAAGAGT GGTTTATGTG TCTCTGGAGA GTCTGTGCGG CCGCAGCGTG 
     2221 GGCCCAACAC CCGTCAACGG GACGGACATT TTAAGAAACG TTCTCGGCCA GATCTCCGAA 
     2281 CCAGATCCAG AAGGAATATA TACAAAAAAC AGGAGCCAGA ACAAGCAGGG GTTGCTAAAG 
     2341 ATGCAAGTGC TGCACCAGAC GTCTCACTCT CTAAAGATAC TAAAACCGAC TTAGCAGGGG 
     2401 TGAACAGTAC CCCTGGGCCA GATGTGTCCT CAGCAACATC TGGACAGGAG GGTCCCAAGT 
     2461 GTCCCAGTGA ACCTGTGGCT TCCCAGATCC AAGCAGAAAG GGACAACTTG GCATGTGCCT 
     2521 CTGCATCTCC AGACAGAATC CCTACCTTAC CTCAGGACAC TGTGGATCAA GAGACAAAGG 
     2581 ATCAGAAGAG AAAATCCTTT GAGCAGGAAG CCTCTGCATC CTTTCCCGAA AAGAAACCCC 
     2641 GGCTTGAAGA TCGTCAGTCC TTTCGTAACA CAATTGAAAG TGTTCACACC GAAAAGCCAC 
     2701 AGCCCACTAA AGAGGAGCCC AAAGTCCCGC CCATCCGGAT TCAACTTTCA CGTATCAAAC 
     2761 CACCCTGGGT GGCTAAAGGT CGGCCCACTT ACCAGATATG CCCCCGGATC GTCCCCATCA 
     2821 CGGAGTCCTC CTGCCGGGGT TGGACTGGTG CCAGGACCCT CGCAGACATT AAAGCCCGTG 
     2881 CTTTGCAGGC CCGAGGGGCG AGAGGTTACC ACTGCAATCG AGAGACGGCC ACCACTGCCA 
     2941 TCGGAGGGGG GGGTGGCCCG GGTGGAGGTG GCAGTGGGGC CATCGATGAG GGAGGTGGCA 
     3001 GAGACAGCAG CAGTGGTGAT GGTAGTGAGG CCTGTGGCCA CCCTGAGCCC AGGGGAGCCC 
     3061 CAAGCACCTC TGGAGAGAGT GCGTCAGATC TACAGCGAAC ACAACTACTG CCGCCTTGTC 
     3121 CTCTGAATGG AGAGCACACT CCAGCTGAAG CTGCCATGCC CAGAGCCAGA AGAGAAGACT 
     3181 CAGCTTCTCT CAGAAAGGAA GAGAGCTGCC TGTTGAAGAG GGTCCCAGGT GTGCTTACAA 
     3241 GTGGGCTGGA AGATGCCTCT CAACCCCCTA TTGCTCCCAC TGGAGACCAG CCGTGTCAGG 
     3301 CTTTGCCCCC TCTGTCCTCC CAAACTCCAG TGGCCGAGAT GTTAACAGAG CAGCCTAAGT 
     3361 TGCTTCTAGA TGATAGAACT GAGTGTGAAT CTAGTAGAGA AGATCAAGGA CCCACCATTC 
     3421 CCTCAGAGAG TAGTTCTGGA CGGTTTCCAT TGGGAGATCT ATTAGGAGGA GGAAGTGACC 
     3481 AGGCCTTTGA TAATATGAAG GAGCCTGTAA GTATGACACC TACTTTTATA TCTGAATTGT 
     3541 CATTAGCTAA CTACCTACAG GATAGGCCTG ATGATGATGG ATTAGGGCTT GGTGCCACAG 
     3601 GCCTACTCAT AAGGGAAAGT AGTAGACAAG AAGCTTTGAC TGAGGCTTTT GCATCTGGCA 
     3661 GTCCTACCTC CTGGGTACCC ATTCTGTCAA ATTATGAGGT AATAAAAACA TCTGATCCAG 
     3721 AATCCAGAGA AAACATACCA TGTCCGGAGC CCCAGGATGA AAAAGAGTGG GAGAGAGCTG 
     3781 TTCCTCTCAT TGCAGCAACA GAAAGTGTGC CCCAACCTGA GAGCTGCATT TCACATTGGA 
     3841 CACCTCCTCC AGCAGCTGTG GGCAGCACTG GCAGTGACAG TGAGCAAGTG GACCTTGAAA 
     3901 GACTGGAAAT GAATGGCATC TCTGAAGCAC CAAGTCCTCA CAGTGAATCC ACAGATACAG 
     3961 CCTCTGACTC CGAAGGCCAT CTCTCTGAGG ACAGCAGTGA GGTTGATGCA AGTGAAGTCA 
     4021 CAGTGGTAAA AGGGTCATTA GGTGGGGATG AAAAGCAAGA CTGGGACCCA TCTGCCTCAC 
     4081 TGTCCAAGGT GAACAATGAC CTAAGTGTGC TTACAAGGAC AGGAGGGGTG GCTGCTTCTC 
     4141 AGAGCTGGGT GTCTAGAGTA TGTTCAGTCC CACACAAGAT CCCAGACTCT CTGTTGCTGT 
     4201 CCAGTACTGA GTGCCAGCCG AGGTCTGTGT GCCCACTGAG GCCTGGCTCT TCAGTGGAGG 
     4261 TTACCAACCC ACTTGTGATG CACCTGCTGC ATGGTAATTT GCCCTTGGAG AAGGTTCTTC 
     4321 CTCCAGGTCA CAGAAGCAGC CGACTAGAGT CATCACAGCT GCCACTTAGA GAACAGAGCC 
     4381 AGGATAGAGG CACTCTACAA GGTACAGGGG AAAACAATCG CCTAGCTGCC AGAATCAACC 
     4441 CTGGTTCTGC ACAAACATTG AAAGAGTCTA TTCTGGCCCA GAGCTATGGA GCAAGTGCTG 
     4501 GTCTTGTCAG GGCAATGGCC TCCAAGGCTC CTGCAATGTC CCAGAAGATT GCGAAGATGG 
     4561 TTACAAGTTT AGACTCACAG CATCCAGAGA CAGAACTGAC ACCTTCCTCT GGCAATCTGG 
     4621 AAGAAATAGA TTCCAAAGAG CATCTCTCTT CCTTCCTTTG TGAAGAGCAG AAAGAAGGCC 
     4681 ATTCCCTGTC TCAAGGCAGT GATCCAGGTG CGGCCCCAGG CCAATGTCTA GGAGATCACA 
     4741 CTACCTCCAA AGTGCCATGT TTCTCCTCCA CAAATGTGAG CCTCTCCTTT GGATCTGAGC 
     4801 AGACAGATGG GACCCTGAGT GATCAGAACA ATGCTGGTGG TCATGAAAAG AAACTATTTG 
     4861 GTCCCGGGAA TACAGTTACC ACCCTTCAGT GCCCCAGGTC TGAAGAGCAG ACACCACTAC 
     4921 CTGCTGAGGT CCCTCCAGTG TTTCCCAGTA GGAAGATAGA ACCAAGCAAA AACTCTGTGT 
     4981 CTGGTGGTGT GCAAACTACA AGGGAAAACA GGATGCCCAA ACCACCTCCT GTCTCTGCTG 
     5041 ACAGCATCAA GACAGAGCAG ACATTTTTGA GGGATCCTAT TAAGGCAGAT GCAGAGAACA 
     5101 GAAAAGCTGC AGGGTACAGT TCTCTGGAAC TAGTGGGTCA CTTGCAAGGG ATGCCTTTTG 
     5161 TTGTGGATCT GCCTTTCTGG AAGTTACCCA GAGAGCCAGG GAAAGGGTTC AGTCAACCCC 
     5221 TGGAGCCTTC TTCCATCCCT TCCCAACTCA ACATCAAGCA GGCCTTGTAT GGGAAGTTGT 
     5281 CTAAACTTCA GCTCAGTCCC ACCAGCTTTA ATTACTCCTC TAGCTCTGCT ACCTTTCCCA 
     5341 AAGGCCTTGC TGGTGGTGTG GTGCAGCTGA GCCACAAAGC CAGCTTTGGT ACAGGCCACA 
     5401 CTGCATCACT GTCCTTACAA ATGTTCGCTG ACAGCAGTGC AGTAGAAAGC ATCTCTCTCC 
     5461 AATGTGCATG CAGCCTGAAA GCCATGATCA TGTGCCAAGG CTGCGGAGCA TTCTGCCATG 
     5521 ATGACTGCAT TGGACCTTCA AAGCTCTGTG TATTGTGCCT TGTGGTGAGA ACGCGTACGC 
     5581 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5641 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5701 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5761 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5821 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5881 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5941 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     6001 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     6061 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     6121 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     6181 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     6241 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     6301 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     6361 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6421 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6481 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6541 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6601 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6661 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6721 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6781 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6841 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6901 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6961 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     7021 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     7081 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     7141 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     7201 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     7261 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     7321 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     7381 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7441 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7501 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7561 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7621 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7681 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7741 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7801 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7861 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7921 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7981 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     8041 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     8101 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     8161 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     8221 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     8281 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     8341 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     8401 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8461 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8521 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8581 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8641 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8701 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8761 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8821 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8881 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8941 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     9001 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     9061 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     9121 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     9181 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     9241 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     9301 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     9361 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9421 TTT
//