LOCUS       MR215637                7296 bp    DNA     circular  SYN 
DEFINITION  Osbp (Myc-DDK-tagged) - Mouse oxysterol binding protein (Osbp)
ACCESSION   NM_001033174
VERSION     NM_001033174.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3453..3544 
                     /label="Myc-DDK"  
     misc_feature    3574..4159 
                     /label="PolyA signal"  
     misc_feature    3631..3650 
                     /label="Restriction Enzyme"  
     misc_feature    4435..5107 
                     /label="ColE1"  
     misc_feature    5537..6331 
                     /label="Kan/Neo"  
     misc_feature    6366..6668 
                     /label="SV40 ori"  
     misc_feature    6754..7231 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3435..3441 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3435 
                     /label="Osbp"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGACC GAGCTGAGAG GAGTGGTGGG GCCGGGCCCG GCAGCCATCG 
     1081 CGGCTCCGGG CGGCGGCGGC GCGGGTCCAC CCGCGGTGGG AGGCGGCGGC GGACGCGGGG 
     1141 ACGCGGGGCC CGGCCCCGGG GTGGCGGCCG CGACGGCGGC GACGGCGGGC GGCCCGGGCC 
     1201 CCGGGGCCGG AGGAGTGGCG GCAGGCGGCC CGGGCTCTGC ACCGCCAGCC GCGGGCTCGG 
     1261 GCGGTTCGGG CGCCGGGGGC TCGGGCTCGG CTAGAGAGGG CTGGCTCTTC AAATGGACCA 
     1321 ATTACATCAA AGGCTACCAG CGGCGATGGT TCGTGCTGAG CAACGGGCTC CTAAGCTACT 
     1381 ACAGGTCCAA GGCAGAGATG AGACACACCT GCCGAGGTAC CATCAACCTC GCCACAGCCA 
     1441 ACATCACTGT GGAGGACTCT TGCAACTTCA TCATCTCCAA TGGCGGTGCT CAGACCTACC 
     1501 ACTTGAAGGC CAGCTCCGAA GTAGAGCGTC AGCGCTGGGT GACAGCCTTA GAACTGGCCA 
     1561 AGGCCAAGGC TGTGAAGATG CTGGCCGAAT CAGATGACTC AGGAGATGAA GAGTCTGTCT 
     1621 CACAGACTGA CAAGACCGAG CTCCAGAGCA CCCTGCGAAC ACTCTCCAGC AAAGTGGAGG 
     1681 ACCTCAGCAC GTGCAACGAC TTGATAGCTA AGCATGGCAC CGCCCTGCAG CGTTCCCTCA 
     1741 GTGAGCTGGA GTCCCTGAAG CTGCCTGCTG AGAGCAATGA GAAAATCAAG CAGGTCAATG 
     1801 AACGAGCCAC GCTTTTTAGG ATAACATCCA ATGCCATGAT CAATGCTTGC AGAGATTTCC 
     1861 TCATGTTAGC CCAGACACAC AGTAAGAAGT GGCAGAAGTC ACTACAGTAT GAAAGAGACC 
     1921 AGCGAATCCG ACTGGAAGAA ACTCTTGAGC AGCTGGCAAA GCAGCACAAT CACCTGGAGA 
     1981 GAGCCTTCCG GGGAGCCACA GTGCTGCCAG CAAACCCTCC GGGCAGCGCT GGGTCTGGTA 
     2041 AAGATCAGTG CTGTTCTGGC AAAGGGGACA TGAGCGATGA AGATGACGAG AATGAGTTTT 
     2101 TTGATGCACC AGAGATTATC ACCATGCCTG AAAATTTGGG CCACAAACGT ACTGGCAGCA 
     2161 ATATCAGTGG GGCCAGCAGT GATGTCAGCC TTGATGAACA GTACAAGCAT CAGCTGGAGG 
     2221 AGACCAAGAA GGAAAAGAGA ACCAGAATAC CATATAAGCC AAACTACAGC CTCAACTTGT 
     2281 GGAGCATCAT GAAGAACTGC ATTGGAAAGG AGCTCTCTAA GATCCCAATG CCGGTAAACT 
     2341 TTAATGAGCC CTTGTCCATG CTTCAGCGCC TTACTGAAGA TCTGGAGTAC CATGAGCTGT 
     2401 TAGACAGAGC TGCAAAGTGT GAGAACTCTC TAGAACAGCT CTGCTATGTT GCAGCCTTCA 
     2461 CTGTTTCCTC CTATTCCACT ACCGTCTTCC GAACCAGCAA GCCATTCAAC CCTCTCCTTG 
     2521 GGGAGACATT TGAACTGGAC CGACTGGAGG AGAATGGGTA TCGGTCCATC TGTGAGCAGG 
     2581 TGAGTCATCA TCCCCCTGCT GCTGCGCACC ATGCTGAATC CAAAAATGGC TGGACATTGC 
     2641 GTCAGGAAAT TAAAATCACC AGCAAGTTTC GAGGCAAATA CCTCTCCATT ATGCCCCTTG 
     2701 GTACTATCCA CTGCATTTTC CACTCAACTG GGCACCATTA CACTTGGAAA AAAGTTACCA 
     2761 CAACAGTACA CAACATTATT GTGGGCAAGT TGTGGATAGA TCAGTCTGGT GAGATTGACA 
     2821 TTGTAAATCA CAAGACAGGA GACAAGTGTA ATCTTAAATT TGTTCCTTAT AGCTACTTCT 
     2881 CTCGGGATGT AGCAAGAAAG GTGACAGGTG AAGTGACAGA CCCATCAGGA AAAGTTCACT 
     2941 TTGCCCTCCT GGGGACATGG GATGAGAAAA TGGATTGTTT CAAAGTACAG GCAGCCTCCG 
     3001 GGGAGAATGG GGGTGATGCT CGGCAGAGAG GCCATGAAGC AGAAGACAGC AGGGTCATGC 
     3061 TGTGGAAGAG GAACCCATTA CCGAAGAATG CAGAGAACAT GTACTACTTC TCAGAGCTTG 
     3121 CCCTCACTCT CAATGCCTGG GAAGGTGGCA CTGCTCCCAC GGACAGCCGG CTGCGGCCCG 
     3181 ACCAGAGACT CATGGAAAAC GGGCGTTGGG ATGAAGCAAA TGCTGAGAAG CAGCGCCTGG 
     3241 AGGAGAAACA GCGGCTTTCC AGGAAGAAGA GAGAAGCAGA AGCTGCGAAA GCCACGGAAG 
     3301 ATGGCACACC ACATGACCCC TATAAAGCAC TGTGGTTTGA GCGGAAGAAG GACCCTGTCA 
     3361 CCAGGGAGCT AACCCATATT TATAGTGGCG AGTACTGGGA ATGTAAGGAA AAGCAGGACT 
     3421 GGGGTTCTTG CCCAGATATT TTCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3481 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3541 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3601 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3661 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3721 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3781 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3841 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3901 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3961 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4021 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4081 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4141 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4201 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4261 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4321 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4381 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4441 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4501 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4561 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4621 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4681 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4741 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4801 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4861 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4921 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4981 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5041 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5101 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5161 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5221 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5281 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5341 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5401 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5461 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5521 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5581 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5641 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5701 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5761 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5821 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5881 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5941 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6001 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6061 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6121 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6181 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6241 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6301 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6361 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6421 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6481 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6541 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6601 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6661 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6721 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6781 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6841 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6901 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6961 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7021 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7081 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7141 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7201 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7261 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//