LOCUS       MR215464                8259 bp    DNA     circular  SYN 
DEFINITION  Amot (Myc-DDK-tagged) - Mouse angiomotin (Amot)
ACCESSION   NM_153319
VERSION     NM_153319.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4416..4507 
                     /label="Myc-DDK"  
     misc_feature    4537..5122 
                     /label="PolyA signal"  
     misc_feature    4594..4613 
                     /label="Restriction Enzyme"  
     misc_feature    5398..6070 
                     /label="ColE1"  
     misc_feature    6500..7294 
                     /label="Kan/Neo"  
     misc_feature    7329..7631 
                     /label="SV40 ori"  
     misc_feature    7717..8194 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4398..4404 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4398 
                     /label="Amot"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGAAGTTCT GACGATCAGC CAAGTGGAGG AACGACGGTA CTGCAACGGC 
     1081 TGCTTCAAGA GCAGCTTCGC TATGGCAATC CCAGTGAGAA TCGCAGTCTG CTTGCCATAC 
     1141 ACCAGCAAGC CACAGGGAAT AGCTCTCCAT TTTCCACGGG CAGTGGGAAC CAAGGCCCTC 
     1201 AGAATGATGT ATTGAGTTCC CAGGATCACC ACCAGCAGCA GCTTGTGGCA CACCCTGCTC 
     1261 GCCAAGAGCC CCAGGGTCAG GAAATCCAGT CAGAAAACGG CGTCATGGAG AAGCAGCTGT 
     1321 CCCCTCGAAT GCAGAATAAC GAAGAACTCC CAACCTATGA AGAGGCCAAG GTCCAGTCCC 
     1381 AGTACTTCAG GGGCCAGCAG CATGCTAGTG TTGGGGCTGC TTTCTATGTC ACAGGAGTCA 
     1441 CCAACCAGAA GATGAGAACC GAGGGACGCC CGTCAGTCCA GCGCCTCACG CCCGGGAAGA 
     1501 TGCACCAAGA TGAAGGCCTT CGAGATCTTA AGCAAGGGCA TGTCCGATCC TTGAGTGAAA 
     1561 GACTGATGCA GATGTCATTG GCTACCAGTG GAGTTAAAGC CCACCCACCT GTTACCAGTG 
     1621 CTCCTCTCTC CCCACCACAA CCCAATGATC TCTACAAGAA TGCCACCAGT TCCAGTGAAT 
     1681 TCTACAAGGC CCAAGGGCCA CCTCCAAGCC AACATAGTCT GAAGGGCATG GAACACCGAG 
     1741 GTCCCCCGCC AGAATACCCT TTCAAGGGCG TGCCATCCCA GTCTGTAGTG TGCAAGTCCC 
     1801 AAGAGCCAGG GCACTTCTAT AGTGAGCATC GCCTGAACCA GCCAGGGAGA ACAGAGGGGC 
     1861 AACTGATGAG GTATCAGCAC CCCCCTGAGT ATGGGGCAGC CAGGGCAACA CAGGACATCT 
     1921 CCTCGCTGTC TTTGTCAGCC AGGAACTCTC AACCTCATAG CCCTACTTCT TCCCTGACAG 
     1981 CTGGGGCTTC CAGCTTGCCC TTGCTGCAGT CTCCGCCGTC CACTAGATTG CCTCCTGGTC 
     2041 AACACCTGGT CTCGAACCAA GGTGACCACT CTGCTCACCT GTCAAGGCAT CAGCAGCATC 
     2101 TTCTTTCGAG TCAGAGTCAT CAGGGGGACC ACTACCGTCA CGCCCAGGCC AGCCTGACTT 
     2161 CAGCGCAGCA GCAGCCAGGA GAAGCCTACT CAGCTATGCC TCGGGCTCAG CAGTCTGCTT 
     2221 CTTATCAGCC CATGCCAGCT GACCCTTTTG CTATGGTCTC TAGAGCTCAG CAGATGGTTG 
     2281 AGATCCTGTC TGATGAGAAT CGCAACTTGC GGCAGGAGTT GGATGGATGC TATGAGAAGG 
     2341 TGGCGAGACT GCAGAAGGTG GAGACAGAAA TCCAACGGGT CTCAGAGGCT TATGAGAACT 
     2401 TGGTGAAGTC ATCTTCCAAA AGAGAGGCTC TGGAGAAAGC CATGAGGAAC AAGCTGGAGG 
     2461 GCGAGATTAG AAGGATGCAT GACTTCAACA GAGATCTGAG AGACCGTCTA GAGACTGCCA 
     2521 ACAAGCAGCT GGCAGAGAAG GAGTACGAGG GGTCCGAGGA CACCAGGAAG ACCATCTCGC 
     2581 AGCTCTTTGC CAAACATAAA GAAAACCAGC GGGAGAAGGA GAAGCTGGAA GCTGAGCTGG 
     2641 CTACTGCCCG TTCTACCAAT GAAGACCAAA GGCGACACAT TGAAATCCGA GACCAGGCAT 
     2701 TGAGCAATGC CCAGGCGAAG GTGGTGAAGC TGGAAGAAGA GCTGAAAAAG AAGCAAGTGT 
     2761 ACGTAGATAA GGTGGAGAAG ATGCAGCAGG CCCTTGTGCA GCTCCAGGCC GCCTGTGAGA 
     2821 AGCGTGAGCA GCTGGAGCAC CGGCTCCGGA CACGTTTGGA GAGGGAACTG GAATCCCTTA 
     2881 GAATCCAGCA GCGCCAGGGC AACTCCCAGC CCACTAATGC TTCAGAATAC AATGCCGCTG 
     2941 CACTGATGGA GCTCCTCCGG GAGAAGGAAG AGAGAATTCT GGCCCTGGAA GCAGATATGA 
     3001 CCAAGTGGGA GCAAAAGTAT TTGGAAGAGA ATGTGATGAG ACACTTTGCT CTGGATGCTG 
     3061 CTGCAACTGT GGCTGCACAG AGAGACACAA CAGTCATTAG CCACTCTCCT AACACCAGCT 
     3121 ATGACACAGC CTTAGAGGCC CGCATCCAGA AGGAAGAGGA AGAAATCCTG ATGGCCAACA 
     3181 AGCGTTGCTT GGACATGGAA GGAAGGATTA AGACCCTCCA TGCCCAGATT ATTGAAAAGG 
     3241 ATGCCATGAT CAAGGTGCTG CAGCAGCGCT CCAGAAAGGA GCCGAGTAAA ACAGAGCAAC 
     3301 TCTCATCCAT GCGGCCAGCA AAGTCTCTGA TGTCCATTTC CAATGCTGGA TCAGGCCTCC 
     3361 TTGCACACTC CTCTACCTTG ACTGGTGCGC CCATTATGGA GGAGAAGCGT GATGACAAGA 
     3421 GCTGGAAGGG GAGCCTAGGC GTTCTTCTGG GAGGAGATTA CCGTGTTGAG CCGGTCCCAT 
     3481 CCACACCGTC CCCTGTGCCC CCGTCTACTC CCCTTCTCTC AGCTCATTCG AAGACAGGCA 
     3541 GTCGCGATTG CAGCACCCAA ACTGAACGTG GACCGGAATC GACCAAAACC GCAGCTGTCA 
     3601 CTCCCATCTC GGCTCCAATG GCTGGTCCAG TTGCTGCTGC TGCCCCTGCT GCTGCCATCA 
     3661 ATGCAACTGC TGCCACCAAC ACTGCCACTG CTGCCACCAA CACCACTATC ATGGTAGCTG 
     3721 CTGCTCCAGT TGCTGTTGCT GCTGTTGCTG CTCCAGCTGC TGCTGCCGCC ACCCCATCTC 
     3781 CAGCAAACGC TGCTGCTCTT GCCGCTGCTG CCGCTCCAGC TACTTCTGTC TCTGCAGCTA 
     3841 CTTCTGTCTC TGCAGCTAAT TCTATCTCTC CAGCTGCTCC TGTCGCTCCA GCTGCTGTAG 
     3901 TGCCTCCAGC TGCTCCTGTC TCTCCAGCTG CTGCTGTTCA GATTCCAGCT GCTGCATCTC 
     3961 TTACTCCTGC TACTGTTTCT CCAACTGCTG CCACTGCTAC TGCTGCTGTT GCTGCAGCTA 
     4021 CTACTGCTGC TATCACTGCT GCTGCTGCTG CTGCCACTAC TGCTATTCAG GTTGCTCCAG 
     4081 CTACTTCGGC TCCGGTTCCA TCTCCAGCTT CGATTCCGGC TCCAGCAACG GCTCAGGCTT 
     4141 CTGCTCCGAC TCCGACTCAG GCATCAACTC CAGCTCCGAC TGAACCTCCT AGTCCAGTTC 
     4201 CAACTCCAAC TCCAGCTTTG GTTCAAACTG AGGGTCCTGC AAATCCAGGT GCCAGTTCCG 
     4261 GACCACGCCG CTTGTCTACA CCAAATCTGA TGTGCAACCC AGATAAGCCA GATGCCCCTG 
     4321 CTTTCCACTC CAGCACTCTG GAAAGAAAGA CACCCATTCA GATCCTGGGG CAAGAGCCTG 
     4381 ATGCAGAGAT GGTGGAATAT CTCATCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4441 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4501 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4561 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4621 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4681 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4741 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4801 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4861 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4921 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4981 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5041 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5101 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5161 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5221 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5281 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5341 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5401 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5461 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5521 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5581 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5641 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5701 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5761 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5821 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5881 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5941 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6001 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6061 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6121 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6181 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6241 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6301 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6361 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6421 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6481 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6541 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6601 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6661 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6721 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6781 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6841 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6901 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6961 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7021 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7081 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7141 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7201 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7261 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7321 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7381 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7441 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7501 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7561 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7681 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7741 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7801 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7861 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7921 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7981 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8041 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8101 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8161 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8221 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//