LOCUS       MR215425                7623 bp    DNA     circular  SYN 
DEFINITION  Tpo (Myc-DDK-tagged) - Mouse thyroid peroxidase (Tpo)
ACCESSION   NM_009417
VERSION     NM_009417.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3780..3871 
                     /label="Myc-DDK"  
     misc_feature    3901..4486 
                     /label="PolyA signal"  
     misc_feature    3958..3977 
                     /label="Restriction Enzyme"  
     misc_feature    4762..5434 
                     /label="ColE1"  
     misc_feature    5864..6658 
                     /label="Kan/Neo"  
     misc_feature    6693..6995 
                     /label="SV40 ori"  
     misc_feature    7081..7558 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3762..3768 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3762 
                     /label="Tpo"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGAACACTT GGAGCTATGG CAATAATGCT GGTGGTTATG GGAACTGTAA 
     1081 TTTTCCTCTC TTTTATCCTG AGAAGCAGAG ACATCTTGTG TGGGAAGACC ATGAAGTCCC 
     1141 ATGTTATCAG TGCTGTGGAA ACGAGCCAGC TCATGGTGGA CCATGCAGTC TACAACACCA 
     1201 TGAAAAGAAA CCTCAAGAAA AGGGAAGTCC TTTCTCCAGC CCAGCTTCTC TCTTTCTTTA 
     1261 AGCTGCCCGA GTCCACCAGT GGGGCTATTT CCCGAGCAGC AGAGATTATG GAAACATCAA 
     1321 TACAAGTCAT GAAACGTGAA CAGTCACAGT TCTCCACGGA TGCCTTATCA GCAGACATTC 
     1381 TGGGCACAAT TGCCAACCTG TCAGGATGCT TGCCTTTCAT GCTGCCACCA AGATGTCCTG 
     1441 ACACCTGCCT GGCAAATAAG TACCGGCCCA TCACAGGGGC GTGCAACAAT AGAGATCACC 
     1501 CCAGATGGGG AGCCTCCAAC ACAGCCCTAG CAAGATGGCT GCCTCCTGTC TATGAAGATG 
     1561 GCTTCAGTCA GCCCAAAGGC TGGAACCCTA ATTTCTTATA CCACGGCTTC CCACTGCCCC 
     1621 CGGTACGGGA AGTGACAAGG CACCTCATTC AAGTTTCCAA TGAGGCTGTG ACCGAAGATG 
     1681 ACCAGTACTC TGATTTTCTG CCGGTGTGGG GACAGTACAT CGATCATGAC ATTGCTCTCA 
     1741 CACCACAGAG CACTAGCACA GCAGCCTTCT GGGGAGGTGT CGACTGCCAG CTGACCTGTG 
     1801 AGAACCAAAA TCCTTGCTTC CCCATACAGC TTCCCTCAAA CTCCTCAGGG ACCACTGCAT 
     1861 GCCTGCCTTT CTACCGCTCC TCCGCCGCTT GTGGCACTGG GGACCAAGGT GCTCTCTTTG 
     1921 GCAACCTGTC TGCAGCCAAT CCGAGGCAGC AGATGAATGG CTTGACCTCC TTCCTTGATG 
     1981 CTTCCACTGT GTACGGCAGC TCCCCTGGCG TTGAGAAGCA GCTGCGCAAC TGGAGCAGCT 
     2041 CGGCAGGACT GCTGCGTGTC AACACTCTCC ACCTAGATGC TGGCCGTGCC TACCTGCCCT 
     2101 TCGCAACAGC CGCCTGCGCT CCAGAGCCTG GTACCCCACG CACCAACCGC ACGCCCTGCT 
     2161 TCCTGGCTGG AGACGGTCGC GCCAGTGAGG TCCCTGCCCT GGCAGCGGTA CACACCTTGT 
     2221 GGCTGCGCGA GCACAACCGC CTGGCTTCGG CCTTCAAGGC CATTAACAAG CACTGGAGCG 
     2281 CCAACACTGC CTACCAGGAG GCGCGCAAGG TGGTAGGGGC ACTGCACCAG ATCATCACCA 
     2341 TGAGGGATTA TATCCCCAAA ATCCTGGGTC CTGATGCCTT CAGGCAGTAT GTGGGCCCCT 
     2401 ATGAAGGCTA CAACCCCACT GTGAACCCTA CTGTGTCCAA CATCTTCTCC ACTGCTGCCT 
     2461 TTCGCTTTGG CCATGCCACA GTCCATCCAC TGGTGAGACG GCTAAACACT GACTTCCAGG 
     2521 AGCACACAGA GCTCCCCAGG TTGCAGCTGC GTGATGTCTT CTTCAGACCC TGGAGGCTTA 
     2581 TCCAGGAAGG TGGTTTGGAT CCGATAGTGA GAGGCCTCCT GGCGAGAGCA GCCAAGCTGC 
     2641 AAGTCCAAGG GCAGCTGATG AATGAGGAGC TGACCGAGAG GCTCTTCGTG CTGTCTAACG 
     2701 TGGGCACCTT GGATCTGGCA TCACTGAACT TGCAGAGGGG CCGGGATCAT GGCTTACCAG 
     2761 ACTACAATGA ATGGAGAGAG TTCTGTGGCT TGTCACGCCT GGAGACACCA GCTGAGCTGA 
     2821 ACAAGGCCAT TGCCAACAGA AGCATGGTCA ACAAGATAAT GGACTTATAC AAGCATGCTG 
     2881 ACAACATTGA CGTCTGGTTG GGAGGCTTGG CTGAAAAGTT CTTGCCGGGG GCCCGCACTG 
     2941 GTCCTCTGTT TGCATGTATC ATTGGGAAGC AGATGAAGGC TCTGAGGGAT GGGGACAGGT 
     3001 TTTGGTGGGA GAACACCAAT GTCTTCACAG ACGCTCAGAG GCAGGAACTA GAAAAGCATT 
     3061 CACTACCTCG GGTCATCTGT GACAACACTG GACTCACCAG AGTACCTGTG GATGCCTTCC 
     3121 GTATTGGAAA GTTCCCCCAA GACTTTGAAT CCTGTGAGGA CATCCCGAGC ATGGACCTTG 
     3181 AACTATGGAG GGAGACCTTC CCACAAGATG ACAAGTGTGT CTTCCCAGAG GAGGTGGACA 
     3241 ATGGGAACTT TGTGCACTGT GAAGAGTCTG GGAAGCTGGT GCTTGTATAC TCCTGTTTCC 
     3301 ATGGATACAA GCTGCAGGGC CAGGAGCAGG TCACATGTAC CCAGAAGGGA TGGGACTCCG 
     3361 AACCTCCTGT CTGTAAAGAT GTTAATGAGT GTGCAGATCT GACACACCCA CCTTGCCACC 
     3421 CCTCTGCACA GTGCAAGAAC ACCAAGGGAA GCTTCCAGTG TGTGTGCACA GACCCCTATG 
     3481 TGCTAGGTGA GGATGAGAAG ACCTGCATAG ATTCTGGCAG GCTACCTCGG GCATCCTGGG 
     3541 TCTCCATTGC ACTGGGTGCA CTTCTCATTG GTGGTTTGGC CAGTCTCACC TGGATAGTAA 
     3601 TTTGCAGGTG GACACATGCT GATAAGAAAG CCACATTGCC GATCACAGAG AGAGTGACCA 
     3661 CCCAGTCAGG ATGCAGAAAG AGTCAGGGGA GGGGGATTTC ACCACACAAG GCCGCAGCTC 
     3721 AAGACACTGG ACAGGAACCT GCAAGTGGAT CCAGGGTCCT CCTGTGCGAA ACGCGTACGC 
     3781 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3841 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3901 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3961 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4021 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4081 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4141 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4201 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4261 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4321 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4381 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4441 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4501 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4561 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4621 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4681 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4741 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4801 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4861 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4921 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4981 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5041 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5101 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5161 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5221 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5281 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5341 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5401 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5461 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5521 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5581 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5641 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5701 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5761 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5821 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5881 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5941 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6001 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6061 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6121 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6181 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6241 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6301 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6361 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6421 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6481 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6541 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6601 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6661 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6721 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6781 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6841 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6901 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6961 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7021 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7081 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7141 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7201 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7261 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7321 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7381 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7441 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7501 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7561 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7621 TTT
//