LOCUS       MR215374                8817 bp    DNA     circular  SYN 
DEFINITION  Kif16b (Myc-DDK-tagged) - Mouse kinesin family member 16B (Kif16b)
ACCESSION   NM_001081133
VERSION     NM_001081133.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4974..5065 
                     /label="Myc-DDK"  
     misc_feature    5095..5680 
                     /label="PolyA signal"  
     misc_feature    5152..5171 
                     /label="Restriction Enzyme"  
     misc_feature    5956..6628 
                     /label="ColE1"  
     misc_feature    7058..7852 
                     /label="Kan/Neo"  
     misc_feature    7887..8189 
                     /label="SV40 ori"  
     misc_feature    8275..8752 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4956..4962 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4956 
                     /label="Kif16b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATCGGTC AAGGTGGCCG TGAGAGTCCG GCCCATGAAT CGCAGGGAAA 
     1081 AGGACCTGGA GGCCAAGTTC ATCATTCAAA TGGAGAAAAG CAAAACGACA ATCACAAACT 
     1141 TAAAGATACC AGAAGGAGGA ACTGGGGATT CAGGCAGAGA ACGGACCAAG ACCTTCACCT 
     1201 ATGACTTCTC TTTTTATTCT GCTGACACCA AGAGTCCAGA TTATGTCTCA CAAGAAATGG 
     1261 TTTTCAAAAC TCTGGGTACA GATGTTGTGA AATCTGCATT TGAAGGTTAC AATGCTTGTG 
     1321 TCTTTGCATA TGGGCAAACT GGATCTGGAA AGTCCTACAC CATGATGGGA AATTCTGGCG 
     1381 ATTCTGGCTT AATACCTCGG ATCTGTGAAG CTCTCTTCAG TCGGATCAAT GAAACCACTA 
     1441 GATGGGATGA AGCCTCTTTC AGGACAGAAG TCAGCTACTT AGAAATTTAT AATGAACGTG 
     1501 TGAGAGACCT ACTTAGGCGG AAGTCATCTA AGACCTTCAA TTTAAGAGTC CGTGAGCATC 
     1561 CCAAAGAAGG ACCGTACGTT GAGGATTTAT CCAAGCATTT AGTCCAGAAC TACAGTGATG 
     1621 TGGAGGAACT CATGGACGCA GGAAATATCA ATCGCACCAC CGCAGCGACG GGGATGAATG 
     1681 ATGTCAGTAG CAGGTCCCAC GCCATCTTCA CCATCAAGTT CACTCAGGCA AAATTCGACG 
     1741 CTGAAATGCC ATGTGAAACT GTGAGCAAGA TCCACCTCGT CGATCTTGCT GGAAGTGAGC 
     1801 GGGCAGATGC CACCGGTGCC ACTGGGGTCA GACTCAAGGA AGGGGGCAAT ATAAACAAGT 
     1861 CCCTTGTGAC TCTGGGAAAT GTCATTTCTG CCTTAGCTGA TTTATCTCAG GATGCAGCCA 
     1921 ACCCTCTTGT GAAGAAGAAG CAAGTTTTTG TGCCTTACAG GGATTCAGTT TTGACTTGGC 
     1981 TGTTGAAAGA CAGCCTTGGA GGAAACTCTA AAACCATCAT GATTGCCACC ATTTCACCTG 
     2041 CTGATGTCAA TTATGGAGAA ACCCTAAGTA CCCTTCGCTA TGCAAATAGA GCCAAAAACA 
     2101 TCATCAACAA GCCTACCATT AATGAGGATG CCAACGTCAA ACTCATCCGT GAGCTGAGAG 
     2161 CTGAAATAGC CAGGCTGAAA ACCCTGCTCG CTCAAGGGAA CCAGATTGCC CTCTTGGACT 
     2221 CCCCTACAGC TTTAAGTATG GAGGAAAAAC TTCACCAGAA TGAAGCAAGA GTCCAAGAAT 
     2281 TGACCAAGGA ATGGACAAAC AAGTGGAATG AAACCCAGAA TATTTTGAAA GAGCAAACTC 
     2341 TAGCCCTCAG GAAAGAGGGC ATTGGTGTGG TGCTGGACTC GGAGCTGCCG CACCTAATTG 
     2401 GCATTGATGA TGATCTTCTG AGCACTGGAA TCATCTTGTA TCACTTGAAG GAGGGTCAGA 
     2461 CTTACGTCGG CAGAGAAGAT GCTTCAACAG AGCAAGATAT TGTCCTTCAT GGCCTTGACT 
     2521 TGGAGAGTGA GCACTGTGTC TTTGAGAACG CCGGGGGAAC AGTGACTCTG ATACCCCTGA 
     2581 GAGGGTCCCA ATGCTCTGTG AATGGTGTTC AGATTGTGGA TGCCACGCAG CTAAATCAAG 
     2641 GTGCTGTGAT ACTCTTGGGA AGAACCAACA TGTTCCGCTT TAACCATCCG AAGGAAGCCG 
     2701 CCAAGCTTAG GGAGAAGAGG AAGAGTGGCC TCCTGTCCTC CTTCAGCTTG TCTATGACCG 
     2761 ACCTCTCAAA GTCCTGTGAG AACCTGTCTG CAGTCATGTT GTATAACCCT GGACTTGAGT 
     2821 TTGAGAGACA ACAGCGTGAA GAACTTGAAA AACTCGAAAG TAAAAGGAAA CTCATTGAGG 
     2881 AGATGGAGGA AAAGCAGAAG TCGGACAAGG CAGAACTAGA GCGAATGCAG CAGGAGGTAG 
     2941 AGACCAGGCG CAAGGAAACA GAGATTGTGC AGCGGCAAAT CCGCAAGCAG GAGGAAAGCC 
     3001 TCAAACGCCG CAGCTTCCAC ATTGAAAACA AGCTCAAGGA CCTGCTGGCT GAGAAGGAAA 
     3061 GGTTTGAGGA AGAGAGGCTT CGGGAGCAGC AGGGACTCGA GCAGCAGAGG AGGCAGGAGG 
     3121 AAGAGAGCTT ATTCCGCATC AGAGAAGAGC TTCGCAAACT CCAAGAGCTC AACAGCCACG 
     3181 AACAAGCTGA GAAGGTCCAG ATATTCCAGG AGCTGGACCG ATTGCACCAA GAGCAAAATG 
     3241 CCCAGAGTGC CAAGCTGCGG TTGGAGAAAA GGAGGCTGGA GGAGGAGGAG AAGGAGCAGG 
     3301 TGCAGCGGGT GGCACACCTG GAGGAGCAGC TTCGGAAGAG GCAGGACACT GCTCCTCTGC 
     3361 TGTGTCCTGG AGAGGCCCAG CGGGCCCAAG AGGAAAAGAG GGAACTGGAA AGCATCCGGG 
     3421 AAGCCCTTTT GCAGGCCAAA GAGATGAGAG CTGGAGGGGA CCATACCTGT AGGGATGAAC 
     3481 TGGAAAGGGC TCAGCAGTAC TTCTTAGAGT TCAAGAGAAG GCAGCTTGTC AAGCTAGCAA 
     3541 GCCTGGAGAA GGACCTGGTC CAACAGAAAG ACCTCCTAAG CAAAGAAGTC CAAGAAGAGA 
     3601 AAGTAGCTCT GGAGCATGTA AAATGTGACG CAGGGGGTGA CCCGAGCTTC TTGGCAACAG 
     3661 ATGATGGCAA CATTCTCGGT GGGCCTCCAG ATCTAGACAA AATAAAGACT GCAGAGACCA 
     3721 GGCTACAGAG CAGAGAGCAC CAGCTACAGG ACCTTCTGCA AAATCATTTG CCAGCTCTGC 
     3781 TGGAAGAAAA GCAGAGAGTG CTTGATGCCC TTGACAGTGG TGTCCTAGGC CTAGACACCA 
     3841 CTCTCTGCCA AGTAGAGAAA GAAGTGGGAG AGAAAGAAGA GCAGATCGCA CAGTACCAGG 
     3901 CTAATGCTAG CCAGCTGCAA CAGCTCCGGG CCACCTTCGA GTTCACAGCT AACGTGGCCA 
     3961 GACAGGAAGA GAAGGTGAGA AGAAAGGAAA AGGAAATACT GGAGTCCCAG GAGAAACAGC 
     4021 AAAGAGAGGC ATTGGAGCAG GCTGTGGCTA AGCTAGAGCA GAGGCGCTCT GCCCTGCAGC 
     4081 GGTGCTCCAC CCTGGACCTG GAGATCCAGG AGCAGAGGCA GAAACTTGGC TCTCTCCACA 
     4141 CCAGTGAATG GTCAGGGTGG CAGGCCAGTC TGGAGACTGA TGGAGAAGCA CTTGAGATGG 
     4201 ACCCTGCAAG GTTAGAACAT GAAATCCATC AGCTGAAGCA GAAGATCTGT GAAGTAGATG 
     4261 GTGTTCAGAG ACCTCATCAT GGGATTTTAG AGGGGCAGGC CGTTCTCTCC AGCTTGCCAC 
     4321 CCAGTGGGGG AAACTCTCAC CTGGCTCCAC TGATGGATGC CAGGATCAGT GCTTACATTG 
     4381 AAGAAGAAGT CCAGAGACGC CTTCATGATT TGCACAGGGC AATTGGTGAT GCCAACCATA 
     4441 CACCTGCTGA TGTGATGAAG AGTAATGAGG AACTTCACAA TGGCACCACT CAACGTAAGC 
     4501 TAAAGTATGA GCGGATGTAT TCTCGCTCTT TGGGAACAAA TCGAGACGAC CTGAAGGACC 
     4561 CCATTAAGAT TAGTATTCCT CGCTATGTCC TGTGTGGCCA AGGGAAGGAC GAGCACTTCG 
     4621 AGTTTGAAGT GAAGATTTCT GTCCTAGATG AGACATGGAC TGTATTCAGG CGCTACAGTC 
     4681 GTTTTCGAGA AATGCATAAA ACATTGAAGC TAAAGTATGC AGAGCTCGCT GCTCTTGAAT 
     4741 TCCCCCCAAA GAAGCTGTTT GGAAACAAAG ACGAGCGGGT AGTTGCTGAG CGGCGGACAC 
     4801 ACTTAGAGAA ATACCTCAGG GAGTTTTTCA GTGTGATGCT CCAGTCCGAG ACATCTCCCC 
     4861 TTCACATCAA CAAAGTGGGA CTGACCCTGT CCAAGCACAC CATTTGTGAA TTCTCACCAT 
     4921 TCTTCAAGAA AGGAGTTTTT GACTACAGCA GCCATGGGAC AGGGACGCGT ACGCGGCCGC 
     4981 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5041 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5101 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5161 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5221 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5281 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5341 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5401 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5461 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5521 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5581 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5641 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5701 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5761 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5821 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5881 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5941 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6001 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6061 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6121 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6181 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6241 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6301 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6361 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6421 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6481 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6541 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6601 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6661 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6721 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6781 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6841 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6901 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6961 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7021 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7081 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7141 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7201 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7261 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7321 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7381 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7441 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7501 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7561 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7621 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7681 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7741 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7801 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7861 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7921 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7981 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8041 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8101 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8161 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8221 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8281 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8341 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8401 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8461 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8521 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8581 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8641 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8701 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8761 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//