LOCUS       MR215322                8496 bp    DNA     circular  SYN 
DEFINITION  Atp2b4 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 4 (Atp2b4), transcript variant x/e
ACCESSION   NM_213616
VERSION     NM_213616.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4653..4744 
                     /label="Myc-DDK"  
     misc_feature    4774..5359 
                     /label="PolyA signal"  
     misc_feature    4831..4850 
                     /label="Restriction Enzyme"  
     misc_feature    5635..6307 
                     /label="ColE1"  
     misc_feature    6737..7531 
                     /label="Kan/Neo"  
     misc_feature    7566..7868 
                     /label="SV40 ori"  
     misc_feature    7954..8431 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4635..4641 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4635 
                     /label="Atp2b4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGAATCCA CCAGGACAAA GCGTGTCAGC CAACACAGTG GCTGAGAGCC 
     1081 ATGAAGGGGA GTTTGGCTGC ACCTTAATGG ACCTGCGAAA GCTCATGGAG CTTCGTGGAG 
     1141 CTGATGCAGT GGCCCAGATC AGTGCCCACT ACGGAGGGGT ACAGGAAATC TGCACTAGAC 
     1201 TGAAAACCTC CCCTATAGAA GGTCTATCTG GGAACCCTGC AGATTTGGAG AAGCGTAGAC 
     1261 TTGTTTTTGG AAAGAACGTG ATACCTCCAA AAAGGCCCAA GACTTTCTTA GAATTAGTGT 
     1321 GGGAAGCCCT GCAGGATGTC ACGCTCATCA TCCTAGAGAT CGCAGCCATC ATCTCCCTGG 
     1381 TCCTGTCCTT CTACCGACCT CCGGGTGGAG ATAATGAAAT CTGTGGTCAC ATTGCAAGTA 
     1441 GCCCAGAAGA AGAGGAGGAA GGAGAAACTG GCTGGATTGA GGGGGCGGCC ATTCTCGCCT 
     1501 CGGTGATCAT TGTGGTACTC GTGACAGCCT TCAATGACTG GAGCAAAGAG AAGCAGTTCC 
     1561 GGGGGCTGCA GAGTCGCATC GAACTGGAGC AAAAATTCTC CATTATCCGG AATGGTCAGC 
     1621 TTATCCAACT CCCAGTGGCT GAGATTGTGG TGGGAGATAT TGCCCAGATC AAATACGGTG 
     1681 ACCTGCTGCC TGCAGATGGA ATTCTAATCC AGGGAAATGA TCTGAAGATT GACGAGAGCT 
     1741 CTCTGACAGG AGAATCAGAT CATGTCAAGA AGACTCTGGA CAAAGACCCC ATGTTGCTCT 
     1801 CGGGGACTCA CGTGATGGAA GGTTCTGGAC GGATGGTAGT GACTGCTGTG GGGGTCAACT 
     1861 CCCAGACTGG AATCATCTTC ACCCTCTTAG GAGCTAGTGA GGAAGAAGAT GATGATGACA 
     1921 AGAAGAAGAA AGGTAAAAAA CAAGGAGCCC CTGAAAATCG CAACAAAGCA AAGACCCAGG 
     1981 ATGGAGTGGC CTTGGAAATC CAGCCACTCA ACAGCCAAGA GGGGCTCGAC AGTGAGGACA 
     2041 AGGAGAAGAA AATAGCCAGG ATACCCAAGA AAGAGAAGTC GGTGCTGCAA GGCAAACTCA 
     2101 CACGCCTGGC TGTGCAGATC GGGAAGGCGG GTCTGATCAT GTCTGTCCTC ACAGTTGTCA 
     2161 TCTTGATTCT GTACTTTGTG GTTGACAATT TCGTGATCCA GCGCCGAGAA TGGCTTCCTG 
     2221 AGTGCACTCC TGTCTATATC CAGTATTTTG TCAAGTTCTT CATCATCGGA GTCACTGTAT 
     2281 TGGTAGTGGC TGTGCCAGAG GGGCTGCCAC TGGCTGTCAC CATCTCACTA GCCTACTCTG 
     2341 TAAAGAAAAT GATGAAGGAC AACAACTTGG TACGGCACTT GGATGCTTGT GAGACAATGG 
     2401 GCAATGCCAC AGCCATTTGC TCCGATAAGA CAGGCACACT GACCATGAAC CGCATGACCG 
     2461 TGGTGCAAGC TTACATCGGG GGCACTCACT ACCGCCAGAT CCCACAGCCC GACGTCTTCC 
     2521 CACCCAAGGT TCTGGAGCTC ATCGTCAATG GCATCTCTAT CAACTGTGCT TACACGTCTA 
     2581 AGATTCAGCC TCCAGAAAAG GAGGGAGGCC TACCCCGACA AGTAGGCAAC AAGACAGAGT 
     2641 GTGGGCTGCT GGGCTTTGTC ACAGACCTGA AGCAGGACTA CCAAGCAGTT CGCAATGAGG 
     2701 TGCCTGAGGA GAAGCTCTTT AAGGTGTACA CCTTCAACTC AGTGCGCAAG TCCATGAGCA 
     2761 CTGTCATCAG GAAGCCGGAA GGGGGCTTCC GCATGTTCAG CAAGGGCGCC TCTGAGATAA 
     2821 TGCTGCGCAG GTGTGATCGA ATCCTGAACA AGGAAGGAGA AATCAAATCG TTCAGAAGTA 
     2881 AGGACCGAGA TAATATGGTA CGCAATGTCA TTGAGCCCAT GGCCAGTGAA GGGCTCCGGA 
     2941 CTATCTGCTT AGCTTACCGA GATTTTGATG GCACAGAGCC CTCGTGGGAC ATTGAGGGTG 
     3001 AAATCCTCAC TTCGCTGATC TGCATTGCAG TGGTGGGCAT TGAGGATCCT GTGCGCCCGG 
     3061 AGGTACCAGA TGCAATTGCC AAATGCAAAC GGGCTGGCAT TACTGTGAGG ATGGTGACAG 
     3121 GTGATAATGT CAACACAGCC CGGGCCATTG CCACCAAATG TGGCATTCTG ACTCCTAAAG 
     3181 ATGACTTCTT GTGCCTGGAA GGCAAAGAAT TCAACAGTCT TATCCGAAAT GAGAAGGGTG 
     3241 AGGTTGAACA AGAAAAGCTG GACAAGATCT GGCCCAAGCT TCGGGTACTG GCACGATCTT 
     3301 CTCCCACAGA CAAGCACACA TTGGTGAAAG GTATCATTGA CAGCACTGCT GGGGAACAGC 
     3361 GGCAGGTAGT GGCTGTCACC GGTGATGGAA CAAATGATGG ACCTGCTCTG AAGAAAGCAG 
     3421 ATGTCGGGTT TGCTATGGGC ATCGCAGGCA CAGATGTGGC TAAGGAGGCG TCAGACATCA 
     3481 TCCTGACCGA TGACAACTTC ACCAGCATTG TGAAGGCAGT GATGTGGGGA CGGAATGTGT 
     3541 ATGACAGCAT TTCCAAGTTC CTACAGTTCC AGCTCACTGT CAATGTGGTG GCCGTGATTG 
     3601 TGGCTTTCAC TGGAGCCTGC ATCACTCAGG ATTCCCCACT GAAAGCGGTG CAGATGTTGT 
     3661 GGGTTAACCT GATTATGGAC ACGTTTGCTT CCCTGGCCCT GGCCACAGAG CCCCCCACCG 
     3721 AGTCTCTGTT GAGGCGGCGC CCCTATGGGC GAAACAAGCC TCTGATCTCC CGCACTATGA 
     3781 TGAAGAACAT CTTGGGCCAT GCTGTGTATC AGCTCCTAAT CGTCTTCCTC CTCGTCTTTG 
     3841 CTGGCGACAC ACTGTTCGAC ATTGACAGTG GGAGGAAGGC ACCTCTCAAT TCGCCACCCA 
     3901 GCCAGCACTA CACCATTGTT TTCAACACGT TTGTGCTGAT GCAACTCTTC AACGAAATCA 
     3961 ACGCTCGGAA GATCCACGGT GAGAAGAACG TGTTTGCAGG CGTCTACCGC AACATCATTT 
     4021 TCTGCACTGT AGTTTTGGGC ACATTCTTCT GCCAGATCAT GATTGTTGAA CTGGGAGGGA 
     4081 AGCCCTTCAG CTGCACAAGC CTGACCATGG AGCAGTGGAT GTGGTGTCTC TTTATAGGGA 
     4141 TTGGAGAACT TTTGTGGGGT CAGGTCATCT CTGCAATACC TACCAAGTCT CTGAAGTTCC 
     4201 TGAAGGAGGC TGGCCACGGC AGTGACAAAG AGGACATCAG CAGGGATACT GAGGGAATGG 
     4261 ACGAGATTGA CCTTGCCGAG ATGGAGCTTC GCCGAGGCCA GATCCTCTGG GTCCGTGGCC 
     4321 TGAACCGGAT TCAGACTCAG ATCAGAGTCG TCAAACTGTT CCATAATAAC CATGAAGTTG 
     4381 CTCATAAACC CAAGAATCGT AGCTCCATCC ACACCTTCAT GACCCAACCT GAATACCCCG 
     4441 CAGATGATGA ATTGTCACAA TCCTTCCTGG ATATCCAAGA GGGCAATCCT GAATTGGTTT 
     4501 CTAAGGCGGG GACGAGTGTG CTCCTGTTGG ATGGTGAAGC TGCTTCGCAC GACAACATAA 
     4561 ATAACAACGC CGTGGACTGC CACCAAGTGC AAATCGTTGC CTCCCATTCT GACAGCCCTC 
     4621 TGCCAAGCCT GGAGACACCG GTCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4681 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4741 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4801 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4861 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4921 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4981 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5041 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5101 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5161 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5221 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5281 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5341 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5401 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5461 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5521 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5581 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5641 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5701 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5761 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5821 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5881 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5941 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6001 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6061 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6121 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6181 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6241 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6301 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6361 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6421 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6481 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6541 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6601 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6661 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6721 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6781 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6841 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6901 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6961 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7021 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7081 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7141 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7201 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7261 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7321 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7381 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7441 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7501 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7561 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7621 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7681 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7741 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7801 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7861 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7921 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7981 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8041 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8101 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8161 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8221 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8281 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8341 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8401 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8461 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//