LOCUS       MR214719                7509 bp    DNA     circular  SYN 
DEFINITION  Ap5b1 (Myc-DDK-tagged) - Mouse predicted gene 962 (Gm962)
ACCESSION   NM_001033448
VERSION     NM_001033448.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3666..3757 
                     /label="Myc-DDK"  
     misc_feature    3787..4372 
                     /label="PolyA signal"  
     misc_feature    3844..3863 
                     /label="Restriction Enzyme"  
     misc_feature    4648..5320 
                     /label="ColE1"  
     misc_feature    5750..6544 
                     /label="Kan/Neo"  
     misc_feature    6579..6881 
                     /label="SV40 ori"  
     misc_feature    6967..7444 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3648..3654 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3648 
                     /label="Ap5b1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCCCCTA GGTCGAGAAG CCTGGGCCCA GCGCCTGGGG GCTTTCCGAG 
     1081 CCAGCCCATC CGCCTTTCTG GCAGGTGCCG AAGGTGAGGA TTTGGGTCGC GACCTGTTGA 
     1141 GTGACCTAAG GAGTGAAAAG CTAAGCGAGC AGACCAAGGT TTCCTTACTG ACACTGAGCT 
     1201 TGGAGTACTC TGATAAACTT TGGCCTGATG CACCTGCCGC GGAGGCTGCT GCCACTTCCC 
     1261 TGTTGGACAC GCTTGTCCTC CTGCCCACAA GACCTTCAGC TCTCCGGAGG CTCCTGTTGT 
     1321 TGGCAGCCAC TACAGCCCTG GTGTCAGGAG ATGCTCTCGG ACCCACTTCG GGAGCTTCCT 
     1381 GCCGGCTTCT GCCCTTACTT CTTGGCTTAG CTTCAGGCCG AGACTTGGGA CGAAGCTTTG 
     1441 GAACCCCTTC AGAACAGCGC CATCTACAGG CCACAGCCTG TGAATGCCTT GGAGAACTTG 
     1501 AGCGCTGTAA GCCTGGGCTG CTGGCCGGCG CCCTGGGGAT GCTGCGGAGC CTCCCAGGGC 
     1561 AAACAGGTCC CATCCAGCCT GTCAGCCTGC TTCTGGCCCT TGTTCTACAC GACACCTTGG 
     1621 TGGTACAGTC CAGGTCTGGG GCTGGCCTGC AAGGCCTGCT GGTGGCTGAG GACTTCTCCA 
     1681 CTGGGAGTTG TCCCTGGGAC TGGACCCTGG CTGAGGAATG GGATGCCCAT CTTAAGCCCC 
     1741 AGGGGCCCAG CTGGCCCACA GCTGGGGAGG AGGAGCGTGG CTTTCCGGTT CTAGAGCCCA 
     1801 GCCCCGAGGA CGCCCGAGAG CTGAAGGCTG CCGTGGCCCA GCTTCTGGAC ACTTCCTATC 
     1861 TGCTCACTCC TGTGGCTCAG GCTCAACTTC TGTGGCTGTT GGGCTGGGCC CTCCGGGGTC 
     1921 TTCGGGGACA GCCACCAGTG CTTTTCAAGC CACAGCTGGT TCGGCTGCTG GGCACGGCAC 
     1981 AGCTGACACT GTTACACTCA GTTCTGTCTC TGAAGGCGGC CTTTGGCGAG GCCCTGTTCA 
     2041 CTGCACAGGA TGAAGCTCTG CTCCTCCGCA GGCTCACCTT GGGAGCCCAG CACCCAGCTT 
     2101 TACCCTCGCC CACACACCTC TTTTACCTGC ATTGCATCCT GAGCTTCCCC GAGAATTGCC 
     2161 CACTAGGTCC AGAGGGGGAA GAGGCTGCCC CGCTGCTGTT GGGGCCCCAG CTGTGCCGGG 
     2221 GCCTCATGCC CAGTCTCCTT CATGACCCAA CGGTTCTCCT GGCCCGCCTG CATTTGCTGT 
     2281 GTCTGCTCTG TGCTGACGAT GAGGAAGAGG AGAAAGACCA GACTCAGGGC CCACAGTGGT 
     2341 TCCTACAGGA GGTGCTGGCT GGGCTACAGC AAAGGGCAGC CCTGGATGGT GGTCCCCGGG 
     2401 CCTTGGCCAC TCTCTGTTTC CAAGCCTCAT ACCTTGTTAC TAACTGCCTG ACTAGACACT 
     2461 CCACAGTACA GACATTTTTG ATCCGCGGAC TGGCCCAACT GTACCGAGCT CGGCCTTCCC 
     2521 TGGCACCCCA CTTTGTGGAC CTCTTAGATC AGGTAAGCCC TGAGCTGAGA GAGCCCCTCA 
     2581 GGGAGGTGCT GCTCCAAGAG GCGGTGGCCA GGCCAGGCGA GAGCGAAGCC CTTTGCTGGC 
     2641 ACCTGCAGAT GCTGGCAAAG GTGGCAGAGG GGGCCACTCA GAGTACCACA CTCAGCTTTC 
     2701 TACAGGCTGC AGCTGTGCAC TGCACCGACT GGGGCCTCCA CCAGGCCCTA CTGCGGGTGT 
     2761 GCCGTGCTCT GCTGCGGACA GGCGGGGGAG ATGGTCTGGC CAACTTGCTG CAGGAACTGG 
     2821 CCAGACAGCT AGAGAATGCT GACGGACGGG ACCATGCCCG ACTATACTAT GTCCTCTTGT 
     2881 CCCATCTGTC AAGTTCCAAG TTGGGGATGG CGCTGGGCCC CTCACTGGCT GCACCTGCAC 
     2941 TGGCCTCGTC ACTAATGGCG GAGAACCAGG GCTTCTCATC AGCACTGATG GTGCAGGAGA 
     3001 CCTCAGCCCC AATTCAGTTG AGTGTGGGGC CTCAGCAGGC CAAAGGCCCG CTCCCGGTGC 
     3061 TGTGTCTGCA GGTGCAGGCA CTGGATGCTC CCGTCTACTC GCTGGAGCTG CGCTTCCGTG 
     3121 TGGAAGGACA GCTCTATGAG CCGTTGGAGG CTGTCCACAT ACCCTGCTTG CGTCCGGGAC 
     3181 AACCTGCGCA TCCTCTGTAC CTGCTTCTGC AGCCCCGATG CCCAGCCCCT GCCCGCCTAC 
     3241 ACGTCCGGGC CCTTTACAGC ACATCCGCTG GCCTCACGTG CCATGCCCGC CTGCCACCCC 
     3301 TGTCTGTGAA CTTTGCTGAC CTTTTCCTGC CTTTCCCCCG GCTCCCTAAG GGCTCTGAGT 
     3361 TGCGTTTCTT CGATGAGCTC TGGAACTCCT GCCTACCAAA GGGCGTAGAG AGCCGTGTGT 
     3421 GGTGTCCACT TGGGCAGCAG GGCCTGGAGG CCTTGGTGTC CCAGTACCTA GAGCCCTTTG 
     3481 TAGTGCTGGC TCAGCCCCCT ACCACCTACC TCATAGCCAT CCGCCTGCCT CCTGCCTCCA 
     3541 TGCTGCTGCT GCGCCTGGAG AAAGCTCAGG TGGATGGTGT GCCTGTAGCC CTAAGGACTG 
     3601 ATGACTGGGC TGTGCTGCCC CTGGTAGGGG ACTACCTCCG CGGGCTTGCA GCCCACACGC 
     3661 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3721 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3781 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3841 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3901 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3961 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4021 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4081 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4141 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4201 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4261 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4321 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4381 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4441 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4501 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4561 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4621 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4681 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4741 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4801 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4861 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4921 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4981 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5041 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5101 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5161 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5221 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5281 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5341 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5401 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5461 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5521 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5581 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5641 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5701 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5761 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5821 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5881 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5941 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6001 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6061 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6121 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6181 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6241 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6301 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6361 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6421 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6481 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6541 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6601 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6661 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6721 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6781 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6841 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6901 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6961 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7021 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7081 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7141 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7201 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7261 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7321 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7381 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7441 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7501 GCGAATTTT
//