LOCUS       MR214463                7287 bp    DNA     circular  SYN 
DEFINITION  Pcdhb16 (Myc-DDK-tagged) - Mouse protocadherin beta 16 (Pcdhb16)
ACCESSION   NM_053141
VERSION     NM_053141.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3444..3535 
                     /label="Myc-DDK"  
     misc_feature    3565..4150 
                     /label="PolyA signal"  
     misc_feature    3622..3641 
                     /label="Restriction Enzyme"  
     misc_feature    4426..5098 
                     /label="ColE1"  
     misc_feature    5528..6322 
                     /label="Kan/Neo"  
     misc_feature    6357..6659 
                     /label="SV40 ori"  
     misc_feature    6745..7222 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3426..3432 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3426 
                     /label="Pcdhb16"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCCAGC AGGAAGCTCA TTTGCAGACA AAGGCAAGTC CTTTTTTTCT 
     1081 TTCTCCTATT AGGCTTCCCT CTAACGACTG CAGGGGAACT GAGGCGCTAT TCTGTGGTGG 
     1141 AGGAAAAGGA GGGTAGGTCC TTTGTAACCA ATTTAGTAAA AGACCTGGGT CTTGGGCAGC 
     1201 TGGAACTCTC CCGGAGAGGA GCTAAGGTCA TTTCTAAAGG TAACAAACTA CATTTGCAGC 
     1261 TGGATCAGGA GACAGGGGAT TTGCTGTTAA ATGAGAAAGT GGACAGGGAA AAACTGTGTG 
     1321 GGCATACAGA GCCATGCTTG CTAAGCTTCC AAGTGTTGCT AGACAACCCC TTGGATATTT 
     1381 TTCAAGCTGA GCTAGAAGTC ACAGACATAA ATGACCACTC ACCAGAATTC CTGGACAAAG 
     1441 AGATCATGCT AAAGATATCA GAAAGCAGTC TTCCTGGAGC TACATTTCCT CTGAAGAATG 
     1501 CTCAGGACAT GGATGTAGGC CAAAACGGTA TTGACAAGTA TCTGATCAGT TCCAATTCCT 
     1561 ATTTTCGAGT GCTCACTCGG AAACGAAGCG ATGGCAAGAA ATACCCTGAA CTGGTACTAG 
     1621 ACAGAGCACT GGATAGAGAG GAGGAAGCTG AGCTCAGGCT AACCCTCACA GCACAGGATG 
     1681 GTGGCTCTCT GCCTAGGTCT GGAACCACTG AAGTCCACAT TGAAGTCCTG GACATCAATG 
     1741 ATAACGCTCC TCAATTTGAG CAGCTTTTTT ACAAAGCACA GATCCCTGAG GATAGTCCAA 
     1801 TAGGCTTTCT GATCATCACT GTCTCTGCTA CAGACAAGGA CATTGGAGTC AATGGACAGA 
     1861 TCTCCTATTC ACTTTTCCAG GTTTCAGATG ATATTAGCAA AACATTTTCA ATCCACCCCT 
     1921 TGACAGGGGA AGTGCGATTG AAAGAACACC TTGATTTTGA AAAGACTCAG TCCTATGAAA 
     1981 TCAATATTGA AGCAAGAGAC GCTGGGACCT TTTCTGGAAA ATGCACGATT CTGGCCCAAG 
     2041 TCTTGGATGT GAATGACCAT GCTCCAGAGA TTATCCTGTC TGCATTTACC AACTCCATTC 
     2101 TTGAAAACTT ACCAGAAACC ATGGTGGCAG TTTTTAGCGT TTCTGATCTA GATTCCGGAG 
     2161 AAAATGGGAA AGTAAGTTGT TCCATTCAGG ACGATCTACC CTTCTTCCTA AAGCCTTCTG 
     2221 GGGAAAACTT TTATACGCTG TTATCACAAA AACCACTGGA CAGAGAGAGT GTAGCAGAAT 
     2281 ACAACATCAC CATCACCGTT GCAGACATGG GGAGTCCCAT CCTGAAAACA CAAGTCAACT 
     2341 TAACAGTGCA GGTATCTGAC ATCAACGACA ACGCCCCCAT CTTCACCCAA ACCTCCTATA 
     2401 CCATGTTCAT CCGTGAGAAC AACAGCCCTG CCCTGCACAT AGGCACCATC AGTGCCACAG 
     2461 ACTCAGACTC AGGCTCCAAT GCTCACATCA CTTACTCGCT GCTGCCGCCC CATGACCACC 
     2521 AGCTGGCCCT CGCCTCGTTC ATCTCCATCA ACGCAGACAA TGGGCAGCTG TTCGTGCTCA 
     2581 GGGCACTGGA CTATGAGGCC CTGCAGGCCT TTGAATTCCA TGTGGGTGCC ACAGACGGAG 
     2641 GTTCGCCCGC TCTCAGCAGC CAGGCGCTGG TGCGCATGGT GGTGCTGGAC GACAATGACA 
     2701 ATGCGCCCTT CGTGCTCTAC CCGATGCAGA ACGCCTCTGC ACCCTACACT GAGCTGCTGC 
     2761 CCAGGGCGGC AGAGCCCGGA TACCTGGTCA CCAAGGTGGT GGCTGTGGAC CGCGACTCTG 
     2821 GACAGAATGC CTGGCTGTCG TTCCAGCTGC TCAAAGCTAC GGAGCCCGGG CTGTTCAGTG 
     2881 TGTGGGCACA CAATGGAGAG GTACATACCT CCAGGCTGCT GAGCGAGCGC GATGTGCACA 
     2941 AGCACAGGCT GATACTGCTA GTCAAGGACA ATGGCGATCC TCCGCGCTCT GCCAGTGTCA 
     3001 CACTGCATGT GCTGCTGGTG GATGGCTTCT CTCAGCCCTA CCTGCCTCTG CCAGAGGTGG 
     3061 CACGTGATCC TGATCATGAG GACAGTGAGC TCACATTGTA CCTGGTCATA GCCTTGGCTT 
     3121 CTGTGTCTTC TCTCTTCCTG GTGTCTGTGC TGCTGTTTGT GGGGGTGAGG CTGTGCAGGA 
     3181 GAGCCAGGGA GGTCTCTCTG GGTGGCTGCT CTGTGCCTGA GGGACACTTT CCTGGCCATC 
     3241 TGGTGGATGT CAGTGGCACA GGGACCCTGT CTCAGAGCTA CCAATATGAG GTGTGTCTTA 
     3301 CTGGAGACTC TGGGACTGGT GAGTTCAAAT ATCTGAAACC AATTTTACCT AATTTTCAAG 
     3361 ACCATTCTTT TAGACCAGAA ATGGGTGAAA ATCCCAACTC TAGGAATGAC TGGAGTTTTG 
     3421 GTATTCAGTT AAAAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3481 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3541 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3601 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3661 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3721 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3781 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3841 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3901 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3961 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4021 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4081 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4141 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4201 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4261 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4321 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4381 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4441 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4501 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4561 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4621 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4681 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4741 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4801 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4861 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4921 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4981 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5041 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5101 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5161 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5221 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5281 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5341 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5401 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5461 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5521 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5581 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5641 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5701 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5761 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5821 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5881 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5941 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6001 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6061 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6121 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6181 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6241 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6301 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6361 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6421 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6481 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6541 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6601 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6661 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6721 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6781 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6841 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6901 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6961 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7021 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7081 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7141 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7201 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7261 ATTTAACAAA AATTTAACGC GAATTTT
//