LOCUS       MR212206                7365 bp    DNA     circular  SYN 
DEFINITION  Mcc (Myc-DDK-tagged) - Mouse mutated in colorectal cancers (Mcc), transcript variant 2
ACCESSION   NM_001085374
VERSION     NM_001085374.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3522..3613 
                     /label="Myc-DDK"  
     misc_feature    3643..4228 
                     /label="PolyA signal"  
     misc_feature    3700..3719 
                     /label="Restriction Enzyme"  
     misc_feature    4504..5176 
                     /label="ColE1"  
     misc_feature    5606..6400 
                     /label="Kan/Neo"  
     misc_feature    6435..6737 
                     /label="SV40 ori"  
     misc_feature    6823..7300 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3504..3510 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3504 
                     /label="Mcc"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATTCTGGA GTTGCGGTGA AATATGGCAA CGACTCCTCA GCCGAGCTGA 
     1081 GCGAGCTCCA CTTGGCAGCC CTGGCATCAC TCAAGGGAGA CATTGTAGAG CTGAATAAGC 
     1141 GTCTGCAGCA GACAGAGCGG GAACGCGACC TCCTGGAAAA GAAGTTGGCC AAAGCACAGT 
     1201 GTGAGCAGTC ACACCTCATG AGAGAGCATG AAGACGTCCA GGAGCGCACG ACACTCCGCT 
     1261 ATGAGGAGCG CATCACAGAA CTCCACAGTA TCATTGCAGA ACTCAACAAG AAGATAGACC 
     1321 GCCTGCAAGG TACCACCATC AGGGAGGAAG ATGAGTACTC AGAACTTCGG TCAGAGCTCA 
     1381 GCCAGAGTCA ACAAGAGGTT AATGAAGACT CCAGAAGTGT GGACCAAGAC CAGACCTCTG 
     1441 TGTCCATCCC TGAGAACCAG TCTACTATGG TCACTGCTGA TATGGATAAC TGCAGTGACT 
     1501 TGAACTCAGA ACTGCAGAGG GTGTTGACCG GTCTGGAGAG TGTCGTCTGC GGCAGGAAGA 
     1561 AGAGCAGCTG TAGCCTCTCT GTGGCCGACG TTGACAGGCA CATAGAGCAG CTCACCACGG 
     1621 CCAGCGAGCA CTGCGACTTG GCCATTAAGA CAGTTGAGGA GATCGAGGGC GTTCTAGGTC 
     1681 GCGATCTGTA TCCCAACCTG GCTGAGGAGC GGTCCCGGTG GGAGAAAGAG CTGGCTGGAC 
     1741 TGCGGGAAGA GAACGAAAGC CTGACGGCCA TGCTATGCAG CAAAGAGGAG GAGCTGAACC 
     1801 GGACCAAGGC CACTATGAAC GCCATCCGTG AGGAGCGGGA CAGGCTTCGG AGGCGGGTCC 
     1861 GAGAGCTCCA AACTCGGCTG CAGAGTGTGC AGGCTACTGG ACCTTCTAGC CCAGGTCGCC 
     1921 TCACCCCTGC CAATCGCCCA ATCAACCCCA GCACCGGAGA GCTAAGCACC AGCAGTAGCA 
     1981 GCAATGACAT CCCCATCGCT AAGATTGCTG AGAGAGTGAA GCTGTCAAAG ACGAGATCTG 
     2041 AATCGTCATC ATCTGATAGG CCAGTCCTGG GCTCGGAAAT CAGTAGCATC GGGGTTTCCA 
     2101 GCAGTGTGGC AGAACACCTG GCCCACTCAC TCCAGGACTG CTCCAACATC CAGGAAATTT 
     2161 TCCAGACACT CTACTCACAC GGATCTGCCA TCTCTGAAAG CAAGATTAGA GAGTTTGAGG 
     2221 TGGAAACGGA ACGATTGAAT AGTCGTATTG AACACCTCAA ATCCCAAAAT GACCTTCTCA 
     2281 CCATAACCCT GGAGGAATGT AAAAGCAACG CCGAGAGGAT GAGCATGCTG GTGGGGAAGT 
     2341 ACGAATCCAA CGCCACAGCC CTGAGGCTGG CCTTGCAGTA CAGTGAGCAG TGCATAGAAG 
     2401 CCTATGAACT CCTGCTGGCC CTGGCTGAGA GCGAGCAGAG CCTCATCCTG GGGCAATTCC 
     2461 GAGCAGCTGG TGTGGGCTCC TCCCCTGGAG ACCAGTCAGG GGATGAAAAT ATCACTCAGA 
     2521 TGCTCAAGCG GGCTCATGAC TGCCGGAAGA CTGCTGAGAA CGCCGCCAAA GCCCTGCTCA 
     2581 TGAAGCTGGA TGGCAGCTGC GGGGGAGCCT TCGCCGTGGC TGGCTGCAGC GTGCAGCCCT 
     2641 GGGAGAGCCT GTCCTCCAAC AGCCATACCA GCACGACCAG CTCTACAGCC AGCAGCTGTG 
     2701 ACACAGAGTT CACTAAGGAA GATGAGCAAA GGTTGAAGGA TTATATCCAG CAGCTCAAGA 
     2761 ATGACAGAGC CGCCGTCAAG CTGACCATGC TGGAGCTGGA GAGCATCCAC ATCGATCCAC 
     2821 TCAGCTATGA TGTCAAGCCA AGAGGCGACA GCCAGAGGCT GGACCTGGAA AATGCCGTGC 
     2881 TGATGCAGGA GCTGATGGCC ATGAAGGAGG AGATGGCTGA GCTGAAGGCC CAGCTCTATC 
     2941 TACTGGAGAA GGAGAAGAAA GCCCTGGAGC TGAAGCTGAG CACGAGGGAG GCGCAGGAGC 
     3001 AGGCCTATCT GGTGCACATC GAGCATCTCA AGTCCGAGGT GGAGGAGCAG AAGGAGCAGA 
     3061 GGACGCGGTC TCTGAGCTCC ACCAGCAGCA GCGGCAAGGA GAAGCCTGGC AAGGAGTGTG 
     3121 CCGATGCTGC CTCTCCAGCT TTGTCACTTG CCGAACTAAG GACGAACAGC GACAGTGAGC 
     3181 TGGCCACCGA GTTCGCCAAT GCCATCCGAC GGGAAAAGAA GCTGAAGGCC AGAGTCCAAG 
     3241 AACTGGTCAG TGCCTTGGAA AGACTAACCA AAAGCAGTGA AATCAGACAC CAGCAGTCAG 
     3301 CAGAGTTTGT TAATGACCTG AAACGGGCCA ACAGCAATCT GGTGGCTGCT TACGAGAAAG 
     3361 CAAAGAAGAA GCATCAAAAC AAGCTGAAGA AGCTGGAATC TCAGATGATG GCCATGGTGG 
     3421 AGCGACACGA GACCCAAGTG AGAATGCTCA AGCAAAGAAT AGCCCTGCTG GAGGAAGAGA 
     3481 ATTCCAGGCC ACACACCAAC GAAACGTCAC TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3541 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3601 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3661 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3721 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3781 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3841 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3901 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3961 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4021 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4081 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4141 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4201 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4261 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4321 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4381 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4441 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4501 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4561 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4621 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4681 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4741 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4801 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4861 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4921 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4981 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5041 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5101 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5161 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5221 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5281 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5341 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5401 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5461 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5521 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5581 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5641 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5701 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5761 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5821 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5881 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5941 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6001 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6061 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6121 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6181 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6241 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6301 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6361 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6421 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6481 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6541 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6601 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6661 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6721 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6781 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6841 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6901 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6961 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7021 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7081 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7141 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7201 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7261 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7321 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//