LOCUS       MR211991                9219 bp    DNA     circular  SYN 
DEFINITION  Pds5b (Myc-DDK-tagged) - Mouse PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae) (Pds5b)
ACCESSION   NM_175310
VERSION     NM_175310.6
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5376..5467 
                     /label="Myc-DDK"  
     misc_feature    5497..6082 
                     /label="PolyA signal"  
     misc_feature    5554..5573 
                     /label="Restriction Enzyme"  
     misc_feature    6358..7030 
                     /label="ColE1"  
     misc_feature    7460..8254 
                     /label="Kan/Neo"  
     misc_feature    8289..8591 
                     /label="SV40 ori"  
     misc_feature    8677..9154 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5358..5364 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5358 
                     /label="Pds5b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCATTCA AAGACAAGGA CCAACGATGG GAAAATTACT TACCCTCCTG 
     1081 GAGTCAAGGA AATCTCAGAT AAAATCTCTA AAGAGGAGAT GGTGAGGCGG TTAAAGATGG 
     1141 TTGTAAAAAC TTTCATGGAC ATGGACCAGG ACTCTGAAGA GGAAAAGGAA CTTTATCTAA 
     1201 ACCTAGCTTT ACATCTTGCT TCTGACTTCT TCCTCAAGCA TCCTGATAAA GATGTTCGTT 
     1261 TACTGGTGGC TTGCTGCCTT GCTGATATTT TCAGGATTTA TGCTCCTGAG GCTCCTTACA 
     1321 CGTCTCCCGA TAAACTAAAG GATATATTTA TGTTTATAAC AAGGCAACTA AAGGGACTAG 
     1381 AAGATACAAA GAGCCCTCAA TTTAATAGGT ATTTTTATTT ACTTGAGAAC ATTGCATGGG 
     1441 TTAAATCATA TAACATATGC TTTGAGTTAG AAGATAGCAA TGAAATCTTT ACTCAACTTT 
     1501 ACAGAACATT ATTCTCAGTT ATAAACAATG GCCACAATCA GAAAGTTCAT ATGCACATGG 
     1561 TGGACCTCAT GAGCTCTATC ATTTGTGAAG GTGATACCGT ATCTCAGGAG CTCTTAGATA 
     1621 CAGTTTTAGT AAATCTGGTA CCTGCCCATA AGAACTTAAA CAAGCAAGCA TATGATTTGG 
     1681 CAAAGGCTTT GCTGAAGAGG ACTGCTCAAG CTATTGAACC ATATATTACC AATTTTTTTA 
     1741 ACCAGGTTCT GATGCTTGGG AAAACATCTA TCAGTGATTT GTCTGAGCAT GTCTTTGATT 
     1801 TGATCTTGGA ACTCTACAAC ATTGACAGCC ATTTGCTGCT TTCTGTTCTG CCCCAGCTTG 
     1861 AGTTTAAATT AAAGAGCAAT GATAATGAAG AACGCCTCCA GGTTGTTAAA CTACTGGCGA 
     1921 AAATGTTTGG GGCAAAGGAT TCAGAATTGG CTTCTCAGAA CAAACCCCTC TGGCAGTGCT 
     1981 ACCTGGGCAG GTTTAATGAT ATCCATGTAC CAATACGCCT GGAATGTGTG AAATTTGCCA 
     2041 GCCACTGCCT CATGAACCAT CCTGATTTAG CAAAGGATTT AACAGAGTAT CTTAAAGTGA 
     2101 GGTCACATGA TCCTGAGGAA GCTATTAGAC ATGATGTTAT TGTGTCTATA GTAACAGCTG 
     2161 CTAAAAAGGA TATTCTTCTT GTCAATGATC ACTTACTCAA TTTTGTGAGA GAGCGAACAT 
     2221 TGGACAAACG GTGGAGAGTA CGTAAAGAAG CCATGATGGG ACTGGCCCAG ATTTATAAGA 
     2281 AATACTCTTT ACAGTCAGCA GCTGGCAAAG ATGCTGCAAA ACAGATATCC TGGGTCAAGG 
     2341 ACAAGCTGCT GCATATATAC TATCAGAACA GCATCGACGA CCGGCTACTT GTTGAGCGGA 
     2401 TCTTTGCTCA GTACATGGTC CCTCACAATT TGGAAACGAC GGAACGGATG AAGTGCTTGT 
     2461 ATTACTTGTA CGCTACACTG GACTTGAATG CTGTGAAAGC ATTGAATGAA ATGTGGAAAT 
     2521 GTCAAAATCT ACTCCGACAT CAAGTAAAGG ATTTACTTGA TTTAATTAAA CAACCAAAAA 
     2581 CAGATGCCAG TGTCAAGGCC ATATTTTCTA AAGTGATGGT TATCACAAGA AATTTGCCAG 
     2641 ATCCTGGTAA GGCCCAAGAC TTCATGAAGA AGTTCACACA AGTGTTGGAA GATGATGAGA 
     2701 AAATACGGAA ACAGCTGGAA GCCCTTGTTA GTCCCACATG TTCCTGCAAG CAGGCTGAAG 
     2761 GCTGTGTGCG TGAAATAACT AAGAAGTTGG GCAACCCTAA GCAGCCTACA AACCCTTTCT 
     2821 TGGAAATGAT CAAGTTTCTC TTGGAGAGGA TAGCTCCTGT ACACATAGAC ACTGAATCCA 
     2881 TCAGTGCTCT TATCAAGCAA GTGAATAAAT CAATAGATGG AACAGCAGAT GATGAGGATG 
     2941 AGGGTGTTCC AACTGATCAA GCTATCAGGG CAGGCCTTGA GCTGCTGAAG GTGCTGTCTT 
     3001 TTACGCACCC TATCTCCTTT CACTCTGCTG AGACGTTTGA GTCCCTTCTG GCTTGTCTGA 
     3061 AGATGGATGA TGAGAAGGTG GCAGAAGCTG CACTACAAAT TTTCAAAAAC ACAGGAAGCA 
     3121 AAATTGAAGA GGATTTCCCT CACATCAGAT CAGCCTTGCT TCCTGTACTA CATCACAAAT 
     3181 CCAAAAAAGG ACCACCTCGG CAGGCCAAAT ACGCCATCCA TTGTATTCAT GCCATATTTT 
     3241 CTAGTAAAGA AACCCAGTTT GCACAGATAT TTGAGCCTCT GCATAAAAGC CTAGATCCAA 
     3301 GCAACCTGGA GCATCTTATA ACCCCTCTGG TCACTATTGG TCATATTGCT CTTCTCGCAC 
     3361 CTGATCAGTT CGCTGCTCCT CTGAAGTCTT TGGTGGCAAC TTTCATTGTG AAGGACCTCC 
     3421 TCATGAATGA CCGGCTTCCA GGAAAAAAGA CAACTAAGCT TTGGGTTCCA GATGAGGAAG 
     3481 TCTCACCTGA GACAATGGTC AAAATTCAGG CTATTAAAAT GATGGTTCGA TGGCTACTTG 
     3541 GAATGAAGAA TAATCACAGT AAGTCAGGAA CTTCCACCCT CAGATTACTA ACAACGATAC 
     3601 TGCATAGTGA TGGGGATTTG ACAGAACAAG GGAAAATTAG TAAACCAGAT ATGTCACGCC 
     3661 TGAGACTTGC TGCTGGGAGT GCTATTGTGA AGCTGGCACA GGAGCCCTGT TACCACGAGA 
     3721 TCATTACACT GGAGCAGTAC CAGCTGTGTG CATTAGCCAT CAATGATGAG TGTTATCAAG 
     3781 TCAGGCAGGT GTTCGCTCAG AAACTTCACA AAGGCCTTTC CCGCTTACGG CTTCCCCTTG 
     3841 AGTACATGGC CATCTGTGCT CTTTGTGCCA AAGACCCTGT GAAAGAGAGG CGAGCCCATG 
     3901 CTAGACAGTG CTTGGTGAAG AACATCACTG TGAGGAGGGA GTACCTGAAG CAGCATGCAG 
     3961 CTGTTAGTGA AAAATTATTG TCTCTTCTAC CAGAGTATGT TGTTCCATAT ACAATTCACC 
     4021 TTTTGGCACA TGACCCAGAT TATGTCAAAG TACAGGATAT TGAACAACTT AAAGATGTGA 
     4081 AAGAATGCCT TTGGTTTGTT CTGGAGATAT TGATGGCTAA AAATGAAAAC AACAGCCATG 
     4141 CATTTATCAG AAAAATGGTA GAAAATATTA AACAGACAAA AGATGCTCAA GGACCAGATG 
     4201 ATACAAAAAT GAATGAAAAA TTGTACACCG TGTGTGATGT TGCCATGAAC ATCATCATGT 
     4261 CCAAGAGCAC CACGTACAGC CTGGAGTCTC CTAAGGACCC CGTGCTGCCA GCTCGGTTTT 
     4321 TCACCCAGCC TGACAAGAAT TTTAGTAACA CCAAAAATTA CCTGCCTCCA GAAATGAAAT 
     4381 CATTTTTCAC TCCTGGAAAA CCTAAAACAG CCAATGTTCT CGGAGCAGTT AATAAGCCAC 
     4441 TTTCATCAGC AGGCAAACAG TCTCAGACCA AATCATCAAG AATGGAAACT GTGAGCAACG 
     4501 CAAGCAGCAG CTCCAACCCA AGCTCTCCTG GAAGGATCAA GGGGAGGCTT GATAGCTCTG 
     4561 AAATGGATCA CAGTGAAAAT GAAGATTATA CAATGTCTTC ACCTTTGCCA GGAAAAAAAA 
     4621 GTGACAAGAG AGAAGACCCT GATCTTTCTG AGTTGGAGAA GCCTAGAAGT CGGAAAAAAG 
     4681 CACCTGTCAC AGACCCTGAA GAGAAATTAG GTATGGATGA CCTAACTAAG TTGGTACAGG 
     4741 AACAGAAACC TAAAGGCAGT CAGCGAGGCC GGAAAAGAGG CCGTACAGCT TCAGACTCAG 
     4801 ACGAGCAGCA GTGGCCTGAG GAGAAGAGGC ACAAAGAGGA GCTCCTGGAA AATGAGGATG 
     4861 AGCAGAACAG CCCACCAAAA AAGGGCAAAA GAGGCAGGCC ACCAAAACCT CTTGGTGGGG 
     4921 GGACATCGAA GGAAGAGCCC ACAATGAAAA CATCCAAGAA AGGAAACAAG AAAAAACTTG 
     4981 TACCTCCTGT AGTAGACGAC GATGAAGAAG AAGAAAGACA AATTGGAAAC ACAGAACATA 
     5041 AGTCAAAAAG CAAACAGCAC CGCACATCAA AGAGAGCACA ACAGAGAGCA GAATCTCCTG 
     5101 AAACAAGTGC AGTTGAATCC ACACAGTCCA CACCACAGAA AGGACGAGGA AGACCATCAA 
     5161 AAGCACCATC ACCATCACAA CCCCCCAAAA AAATCCGTGT AGGACGCTCC AAACAGGTAG 
     5221 CCACTAAAGA AAACGATTCA AGTGAAGAAA TGGATGTGCT TCAGGCCAGC TCTCCTGTCA 
     5281 GTGACGACAC TACACAGGAA GGAGCAGAAG AGGAGGACAT TTCTGTGGGA AATGTACGAC 
     5341 GGCGAAGCTC CAAACGAGAG AGACGAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5401 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5461 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5521 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5581 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5641 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5701 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5761 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5821 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5881 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5941 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     6001 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     6061 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     6121 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6181 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6241 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6301 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6361 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6421 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6481 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6541 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6601 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6661 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6721 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6781 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6841 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6901 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6961 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     7021 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     7081 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     7141 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     7201 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7261 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7321 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7381 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7441 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7501 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7561 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7621 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7681 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7741 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7801 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7861 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7921 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7981 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     8041 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     8101 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     8161 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     8221 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8281 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8341 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8401 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8461 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8521 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8581 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8641 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8701 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8761 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8821 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8881 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8941 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     9001 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     9061 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     9121 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     9181 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//