LOCUS       MR211989                9204 bp    DNA     circular  SYN 
DEFINITION  Cpsf1 (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specific factor 1 (Cpsf1), transcript variant 2
ACCESSION   NM_053193
VERSION     NM_053193.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5361..5452 
                     /label="Myc-DDK"  
     misc_feature    5482..6067 
                     /label="PolyA signal"  
     misc_feature    5539..5558 
                     /label="Restriction Enzyme"  
     misc_feature    6343..7015 
                     /label="ColE1"  
     misc_feature    7445..8239 
                     /label="Kan/Neo"  
     misc_feature    8274..8576 
                     /label="SV40 ori"  
     misc_feature    8662..9139 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5343..5349 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5343 
                     /label="Cpsf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACGCGGTG TACAAGCAGG CGCACCCGCC CACCGGGCTG GAGTTTACCA 
     1081 TGTACTGCAA CTTCTTCAAC AACAGTGAGC GCAACCTCGT GGTGGCCGGC ACCTCGCAGC 
     1141 TCTATGTGTA CCGCCTGAAC CGCGACGCCG AGGCTCTGAC CAAGAATGAT GGGAGCACAG 
     1201 AGGGGAAGGC CCACCGAGAG AAACTAGAGC TGGTAGCCTC CTTCTCTTTT TTTGGCAATG 
     1261 TCATGTCCAT GGCCAGTGTG CAGCTTGCAG GCGCCAAACG TGATGCCTTG CTTCTCAGTT 
     1321 TCAAAGACGC TAAGCTGTCT GTGGTGGAGT ATGACCCAGG CACTCATGAC CTGAAGACCC 
     1381 TGTCTCTACA CTACTTTGAG GAGCCTGAAC TTCGGGATGG GTTTGTGCAG AACGTGCATA 
     1441 CGCCTCGTGT GCGGGTGGAC CCTGACGGGC GCTGTGCAGC AATGCTCATC TATGGCACGA 
     1501 GGCTGGTAGT TCTGCCTTTC CGCAGAGAGA GCCTGGCTGA GGAACATGAG GGGCTCATGG 
     1561 GCGAAGGGCA GAGGTCTAGC TTCTTGCCCA GCTACATCAT TGATGTACGG GCTCTGGATG 
     1621 AGAAACTACT CAACATCATT GACCTGCAGT TCCTGCATGG CTACTATGAG CCCACCCTGC 
     1681 TTATCCTGTT TGAGCCCAAC CAGACTTGGC CAGGGCGGGT GGCTGTGAGG CAGGACACGT 
     1741 GCTCCATTGT GGCTATCTCG CTGAACATCA CACAGAAAGT CCATCCAGTC ATCTGGTCCC 
     1801 TCACCAGCTT GCCTTTTGAC TGTACCCAGG CCCTGGCTGT GCCCAAGCCC ATAGGTGGGG 
     1861 TAGTGATCTT CGCTGTCAAC TCACTGTTGT ACCTGAACCA GAGTGTTCCC CCATATGGTG 
     1921 TGGCTCTCAA TAGTCTCACC ACAGGCACCA CCGCTTTCCC ATTGCGTACC CAAGAGGGTG 
     1981 TACGGATCAC CCTAGACTGC GCACAGGCGG CCTTCATCTC TTATGACAAG ATGGTCATCT 
     2041 CCCTCAAGGG TGGTGAGATT TATGTGCTGA CCCTCATCAC TGACGGCATG CGAAGTGTCC 
     2101 GAGCGTTTCA CTTTGACAAG GCAGCTGCTA GTGTCCTTAC CACCAGTATG GTCACAATGG 
     2161 AGCCCGGATA CCTGTTCCTA GGCTCTCGCC TGGGCAATTC CCTCCTCCTC AAGTACACGG 
     2221 AGAAGCTGCA GGAGCCCCCA GCCAGCTCCG TCCGTGAGGC TGCTGACAAG GAAGAGCCAC 
     2281 CCTCAAAGAA GAAGCGAGTG GAGCCTGCAG TGGGCTGGAC AGGAGGCAAG ACGGTGCCAC 
     2341 AGGACGAGGT GGATGAAATT GAAGTTTATG GCAGTGAGGC CCAGTCAGGC ACACAGCTGG 
     2401 CTACTTACTC TTTTGAGGTA TGTGACAGCA TGCTCAACAT TGGACCCTGT GCCAACGCTG 
     2461 CTGTGGGCGA GCCTGCCTTC CTCTCTGAAG AGTTCCAGAA TAGCCCTGAG CCAGACCTGG 
     2521 AGATTGTGGT TTGTTCCGGC TACGGGAAGA ATGGGGCCCT GTCAGTGTTG CAGAAGAGTA 
     2581 TCCGGCCCCA GGTGGTGACA ACCTTTGAAC TTCCTGGCTG CTATGATATG TGGACAGTCA 
     2641 TTGCTCCTGT GCGGAAAGAG GAGGAAGAAA CCCCCAAAGC AGAGAGCACA GAGCAGGAGC 
     2701 CCAGTGCCCC GAAAGCAGAG GAGGATGGGC GAAGACATGG CTTCCTTATT CTTAGCCGGG 
     2761 AAGACTCAAC CATGATCCTA CAGACTGGCC AGGAGATCAT GGAGCTGGAC ACCAGTGGTT 
     2821 TTGCCACTCA GGGTCCTACT GTCTTTGCTG GAAACATTGG GGACAATCGT TACATTGTTC 
     2881 AAGTCTCGCC ACTGGGCATT CGCCTGTTGG AAGGCGTGAA TCAATTGCAC TTCATCCCTG 
     2941 TGGACCTGGG CGCCCCCATC GTGCAGTGTG CCGTGGCTGA TCCCTATGTG GTTATCATGA 
     3001 GTGCTGAGGG ACACGTTACC ATGTTCCTAC TCAAGAGCGA TTCCTATGGT GGCCGTCATC 
     3061 ACCGCCTGGC ACTACACAAG CCTCCACTGC ATCATCAGTC CAAGGTGATT GCTCTCTGCC 
     3121 TGTACCGCGA TGTCAGTGGC ATGTTCACCA CTGAGAGCCG CCTGGGTGGG GCCCGTGATG 
     3181 AACTGGGTGG CCGAAGTGGC TCCGAGGCAG AAGGCCTGGG CTCAGAAACG AGCCCCACAG 
     3241 TGGATGATGA GGAAGAGATG CTCTATGGGG ACTCCAGTGC CCTCTTCAGT CCCAGCAAAG 
     3301 AGGAGGCCCG TAGGAGCAGC CAGCCACCGG CTGATCGTGA CCCTGCACCC TTCAAGGCAG 
     3361 ACCCCACTCA CTGGTGCCTG CTGGTGCGGG AGAATGGCAC CATGGAGATC TACCAGCTCC 
     3421 CAGACTGGCG GCTGGTATTC CTAGTTAAGA ACTTCCCCGT GGGGCAGCGG GTTCTGGTGG 
     3481 ACAGTTCATT TGGACAGCCC ACTACACAAG GTGAAGTCCG AAAGGAAGAA GCCACACGCC 
     3541 AGGGGGAGCT GCCTTTGGTT AAGGAGGTGT TGTTGGTTGC TCTGGGAAGC CGGCAGAGCA 
     3601 GGCCCTACCT GCTGGTACAC GTGGACCAGG AGTTGCTTAT CTATGAAGCC TTTCCCCATG 
     3661 ACTCTCAGCT TGGCCAGGGA AATCTTAAAG TCCGTTTCAA AAAGGTCCCT CACAATATCA 
     3721 ACTTCCGTGA AAAGAAGCCA AAGCCGTCCA AGAAGAAGGC CGAAGGTTGC AGCACTGAGG 
     3781 AGGGATCTGG AGGCCGGGGC CGTGTGGCAC GTTTCCGCTA CTTTGAGGAT ATTTATGGCT 
     3841 ATTCGGGGGT TTTCATCTGT GGACCCTCAC CTCACTGGCT CCTGGTGACT GGCCGCGGAG 
     3901 CTCTGCGTCT GCACCCCATG GGCATTGATG GCCCCATTGA CTCCTTTGCT CCTTTCCACA 
     3961 ATGTGAACTG CCCCCGTGGT TTCCTGTACT TCAACAGACA GGGGGAGCTG AGGATCAGCG 
     4021 TCCTGCCTGC CTACCTGTCC TACGATGCCC CATGGCCTGT CAGGAAGATC CCACTCCGAT 
     4081 GCACAGCCCA CTATGTGGCC TACCATGTGG AATCCAAGGT CTATGCTGTG GCCACTAGTA 
     4141 CCAACACACC TTGCACTAGG ATCCCACGCA TGACTGGCGA GGAAAAAGAA TTCGAGGCCA 
     4201 TTGAGAGAGA CGACAGATAC ATCCACCCCC AGCAAGAAGC CTTCTCCATC CAGCTCATCT 
     4261 CCCCGGTCAG CTGGGAGGCC ATTCCCAATG CCAGGATCGA GCTAGAGGAA TGGGAGCATG 
     4321 TCACGTGCAT GAAGACAGTG TCACTGCGCA GTGAGGAGAC CGTGTCAGGC CTAAAGGGCT 
     4381 ATGTGGCTGC TGGGACCTGC CTCATGCAGG GGGAGGAGGT CACGTGCCGT GGGCGGATCC 
     4441 TGATCATGGA TGTGATTGAA GTGGTGCCTG AGCCTGGGCA GCCCCTGACC AAGAACAAAT 
     4501 TCAAGGTCCT GTATGAGAAG GAGCAGAAAG GGCCCGTGAC TGCGCTGTGC CACTGCAATG 
     4561 GCCACCTGGT GTCGGCCATT GGCCAAAAAA TCTTCCTGTG GAGCCTGCGG GCTAGCGAGC 
     4621 TGACCGGCAT GGCCTTCATC GACACCCAGC TGTACATCCA CCAGATGATC AGTGTCAAGA 
     4681 ACTTCATCCT GGCAGCAGAC GTCATGAAGA GCATCTCACT GCTGCGTTAC CAAGAGGAGA 
     4741 GCAAGACGCT GAGCCTGGTG TCCCGGGATG CCAAGCCTTT GGAGGTATAC AGCGTGGACT 
     4801 TCATGGTGGA CAATGCCCAA CTGGGCTTCT TGGTGTCTGA CCGAGACCGC AATCTTATGG 
     4861 TGTATATGTA TCTGCCAGAA GCCAAAGAGA GTTTTGGGGG CATGCGCCTG CTACGCAGAG 
     4921 CGGATTTCCA TGTGGGTGCC CACGTGAATA CGTTCTGGAG GACCCCGTGC CGAGGTGCTG 
     4981 CCGAGGGCCC CAGCAAGAAG TCAGTCGTGT GGGAAAACAA GCATATCACA TGGTTTGCAA 
     5041 CCCTTGACGG TGGCATTGGG CTCCTGCTGC CCATGCAAGA AAAGACATAC CGACGCTTGC 
     5101 TGATGCTGCA GAATGCACTG ACGACCATGC TACCCCACCA CGCTGGCCTC AACCCCCGCG 
     5161 CCTTCCGAAT GTTGCACGTA GACCGGCGCA TCTTGCAGAA TGCTGTGCGC AACGTCCTTG 
     5221 ATGGGGAACT GCTAAACCGC TACCTATACC TCAGCACCAT GGAGCGCAGT GAGCTGGCCA 
     5281 AGAAGATAGG TACCACGCCT GACATTATCC TGGATGACTT GCTGGAGACA GACCGGGTCA 
     5341 CGGCTCACTT CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5401 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5461 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5521 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5581 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5641 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5701 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5761 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5821 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5881 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5941 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     6001 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     6061 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     6121 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6181 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6241 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6301 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6361 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6421 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6481 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6541 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6601 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6661 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6721 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6781 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6841 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6901 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6961 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     7021 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     7081 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     7141 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7201 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7261 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7321 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7381 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7441 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7501 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7561 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7621 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7681 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7741 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7801 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7861 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7921 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7981 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     8041 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     8101 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     8161 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8221 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8281 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8341 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8401 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8461 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8521 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8581 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8641 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8701 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8761 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8821 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8881 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8941 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     9001 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     9061 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     9121 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9181 TAACAAAAAT TTAACGCGAA TTTT
//