LOCUS       MR211981                9153 bp    DNA     circular  SYN 
DEFINITION  Kdm6a (Myc-DDK-tagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a)
ACCESSION   NM_009483
VERSION     NM_009483.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5310..5401 
                     /label="Myc-DDK"  
     misc_feature    5431..6016 
                     /label="PolyA signal"  
     misc_feature    5488..5507 
                     /label="Restriction Enzyme"  
     misc_feature    6292..6964 
                     /label="ColE1"  
     misc_feature    7394..8188 
                     /label="Kan/Neo"  
     misc_feature    8223..8525 
                     /label="SV40 ori"  
     misc_feature    8611..9088 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5292..5298 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5292 
                     /label="Kdm6a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAATCCTGC GGAGTGTCGC TCGCTACCGC CGCCGCCGCC GCCGCCGCCG 
     1081 CCGCTTTCGG TGATGAGGAA AAGAAAATGG CGGCGGGAAA AGCGAGCGGC GAGAGCGAGG 
     1141 AGGCGTCCCC CAGCCTGACA GCGGAGGAGA GGGAGGCGCT CGGCGGACTG GACAGCCGCC 
     1201 TTTTCGGGTT CGTGAGGTTT CATGAAGATG GCGCCAGGAT GAAGGCCCTG CTGGGCAAGG 
     1261 CTGTTCGCTG CTACGAATCT CTAATCTTAA AAGCTGAAGG GAAAGTGGAG TCTGATTTCT 
     1321 TTTGTCAATT AGGTCACTTC AACCTCTTAT TGGAAGATTA TCCAAAAGCA TTATCTGCAT 
     1381 ACCAGAGGTA CTACAGTTTA CAGTCTGATT ACTGGAAGAA TGCTGCCTTT TTATATGGTC 
     1441 TTGGTTTGGT CTACTTCCAT TACAATGCAT TTCAGTGGGC TATTAAAGCA TTTCAGGAGG 
     1501 TGCTTTATGT CGATCCCAGC TTTTGTCGAG CCAAGGAAAT TCATTTACGA CTTGGGCTTA 
     1561 TGTTCAAAGT GAACACAGAC TATGAGTCTA GTTTAAAGCA TTTTCAGTTA GCTTTGGTTG 
     1621 ACTGTAATCC CTGCACTTTG TCCAATGCTG AAATTCAGTT TCACATTGCC CACTTATATG 
     1681 AAACCCAGAG GAAGTATCAT TCTGCAAAAG AAGCTTATGA GCAACTTTTG CAGACAGAAA 
     1741 ACCTTTCTGC ACAAGTAAAA GCAACTATTT TACAACAATT AGGTTGGATG CATCACACTG 
     1801 TGGATCTCCT GGGAGATAAG GCCACCAAGG AAAGTTATGC TATTCAGTAT CTCCAGAAGT 
     1861 CCTTGGAAGC AGATCCAAAT TCTGGCCAGT CCTGGTATTT CCTTGGAAGG TGCTATTCAA 
     1921 GTATTGGGAA AGTTCAGGAT GCCTTTATAT CTTACAGGCA ATCTATTGAT AAATCAGAAG 
     1981 CAAGTGCAGA TACATGGTGT TCAATAGGTG TGCTCTATCA ACAGCAAAAT CAGCCTATGG 
     2041 ATGCTTTGCA AGCTTATATT TGTGCTGTAC AATTGGACCA CGGTCATGCT GCAGCCTGGA 
     2101 TGGATCTAGG CACTCTCTAT GAATCCTGCA ACCAACCTCA GGATGCTATT AAATGCTATT 
     2161 TAAATGCAAC TAGAAGCAAA AATTGTAGTA ATACCTCTGG ACTTGCAGCA CGAATTAAGT 
     2221 ATTTACAGGC TCAGTTGTGT AACCTTCCAC AAGGTAGTCT ACAGAATAAA ACTAAATTAC 
     2281 TTCCTAGTAT TGAGGAGGCA TGGAGCCTAC CAATCCCCGC AGAGCTTACC TCCAGGCAGG 
     2341 GTGCCATGAA CACAGCACAG CAGAATACTT CTGATAATTG GAGTGGTGGC AATGCACCAC 
     2401 CTCCAGTAGA ACAACAAACT CATTCATGGT GTTTGACACC ACAGAAATTA CAGCACTTGG 
     2461 AACAGCTCCG AGCAAACAGA AATAATTTAA ATCCAGCACA GAAACTAATG CTGGAACAGC 
     2521 TGGAAAGTCA GTTTGTCTTA ATGCAGCAAC ACCAAATGAG ACAAACAGGA GTTGCACAGG 
     2581 TACGGCCTAC TGGAATTCTT AATGGGCCAA CAGTTGACTC ATCACTGCCT ACAAACTCAG 
     2641 TTTCTGGCCA GCAGCCACAG CTTCCTCTGA CCAGAATGCC TAGTGTCTCT CAGCCTGGAG 
     2701 TCCACACTGC CTGTCCTAGG CAGACTTTGG CCAATGGACC CTTTTCTGCA GGCCATGTTC 
     2761 CCTGTAGCAC ATCAAGAACA CTGGGAAGTA CAGACACTGT TTTGATAGGC AATAATCATG 
     2821 TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA CCCACTCTAC 
     2881 CTCATAACCG CACAAACCTG ACCAGCAGCA CAGAGGAGCC GTGGAAAAAC CAACTATCTA 
     2941 ACTCCACTCA GGGGCTTCAC AAAGGTCCGA GTTCACATTT GGCAGGTCCT AATGGTGAAC 
     3001 GACCTCTATC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT GGTATTCAGA 
     3061 ATCAGAATGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT GCTCTCAATC 
     3121 ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA ACCAAAGAGA 
     3181 GCAAGCCTTC AGGAAACACA TTGACGGTGC CTGAAACAAG CAGGCAAACT GGAGAGACAC 
     3241 CTAACAGCAC TGCCAGTGTT GAGGGACTTC CTAATCATGT CCATCAGGTG ATGGCAGATG 
     3301 CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT TCAGACAATC 
     3361 CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CTAATAACAA TGTGGGTCCT GGAACCTGTG 
     3421 ACAAAGTCAA TAACATCCAC CCAACTGTCC ATACAAAGAC TGATAATTCT GTTGCCTCTT 
     3481 CACCATCTTC AGCCATTTCC ACAGCAACAC CTTCTCCTAA GTCCACTGAA CAGACAACCA 
     3541 CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT GCACACAATT AATGGAGAAG 
     3601 GAATGGAAGA ATCTCAGAGC CCCATTAAAA CAGATCTGCT TCTAGTTAGC CACAGACCTA 
     3661 GTCCTCAGAT CATACCATCA ATGTCTGTGT CCATATATCC CAGCTCAGCA GAAGTTCTGA 
     3721 AAGCTTGCAG GAATCTAGGT AAAAACGGCC TGTCTAATAG TAGCATTCTG TTGGATAAAT 
     3781 GTCCGCCTCC AAGACCACCA TCCTCACCAT ACCCTCCCTT GCCAAAGGAC AAGTTGAATC 
     3841 CACCTACACC TAGTATTTAT TTGGAAAATA AACGTGATGC TTTCTTTCCT CCATTACATC 
     3901 AATTTTGTAC AAACCCAAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT GGAGCTCTTA 
     3961 AATTAGACTT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT GAACATATGG 
     4021 TAGAAGTGAG GACACAGTTG TTACAACCAG CAGATGAAAA TTGGGACCCT ACTGGAACCA 
     4081 AGAAAATCTG GCACTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA TATGCTCAGT 
     4141 ACCAGGCCTC CTCATTCCAA GAATCATTGA GAGAAGAAAA TGAGAAAAGA AGTCACCATA 
     4201 AAGACCACTC AGACAGTGAA TCTACATCAT CAGATAATTC TGGGAAAAGA AGAAAAGGAC 
     4261 CCTTTAAAAC CATTAAGTTT GGGACCAACA TTGACCTGTC TGATGACAAA AAGTGGAAGT 
     4321 TACAGCTACA TGAGCTGACT AAACTTCCTG CCTTCGTGAG AGTTGTATCT GCAGGAAATC 
     4381 TTTTAAGCCA CGTTGGTCAT ACTATACTGG GCATGAACAC AGTTCAACTA TACATGAAAG 
     4441 TTCCAGGAAG CAGAACACCA GGTCATCAAG AAAATAACAA CTTCTGTTCA GTTAATATAA 
     4501 ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGCTACTGG GGTGTTTTGA 
     4561 ATGACTTCTG TGAAAAAAAT AATTTGAATT TCTTAATGGG TTCTTGGTGG CCCAACCTTG 
     4621 AAGATCTATA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT GGAGATCTGG 
     4681 TCTGGATAAA TGCTGGCACT GTTCATTGGG TTCAAGCTAT TGGCTGGTGC AACAATATTG 
     4741 CTTGGAATGT TGGTCCACTT ACAGCCTGTC AGTATAAGTT AGCAGTGGAA CGTTATGAAT 
     4801 GGAACAAGTT GCAAAATGTA AAGTCAATAG TACCCATGGT TCATCTTTCC TGGAATATGG 
     4861 CACGAAATAT CAAGGTTTCA GATCCAAAGC TTTTTGAAAT GATTAAGTAT TGTCTTCTGA 
     4921 GAACGCTGAA GCAATGTCAG ACATTGAGGG AAGCTCTAAT TGCTGCAGGA AAAGAGATCA 
     4981 TATGGCACGG GCGGACAAAA GAAGAACCAG CTCATTATTG TAGTATTTGT GAGGTGGAGG 
     5041 TTTTTGATCT GCTTTTTGTC ACTAATGAGA GTAATTCTCG AAAAACCTAC ATAGTACATT 
     5101 GCCAAGATTG TGCACGAAAA ACAAGTGGGA ATCTGGAAAA TTTTGTGGTG CTAGAACAGT 
     5161 ACAAAATGGA GGATCTGATG CAAGTCTATG ACCAATTTAC ATTAGTAAGT GAAATCAACA 
     5221 TGCTCCTCCA TTACCATCCG CCTCATCTTG ATATTGTTCC ATGGACATTA AACATGAGAC 
     5281 CTTTTCTGCT ATTCAGAAAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5341 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5401 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5461 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5521 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5581 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5641 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5701 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5761 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5821 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5881 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5941 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6001 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6061 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6121 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6181 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6241 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6301 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6361 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6421 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6481 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6541 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6601 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6661 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6721 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6781 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6841 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6901 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6961 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7021 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7081 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7141 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7201 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7261 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7321 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7381 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7441 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7501 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7561 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7621 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7681 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7741 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7801 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7861 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7921 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7981 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8041 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8101 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8161 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8221 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8281 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8341 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8401 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8461 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8521 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8581 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8641 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8701 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8761 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8821 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8881 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8941 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9001 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9061 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9121 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//