LOCUS       MR211977                9135 bp    DNA     circular  SYN 
DEFINITION  Smg6 (Myc-DDK-tagged) - Mouse Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6)
ACCESSION   NM_001002764
VERSION     NM_001002764.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5292..5383 
                     /label="Myc-DDK"  
     misc_feature    5413..5998 
                     /label="PolyA signal"  
     misc_feature    5470..5489 
                     /label="Restriction Enzyme"  
     misc_feature    6274..6946 
                     /label="ColE1"  
     misc_feature    7376..8170 
                     /label="Kan/Neo"  
     misc_feature    8205..8507 
                     /label="SV40 ori"  
     misc_feature    8593..9070 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5274..5280 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5274 
                     /label="Smg6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGGGG TTGGAGCGTG TGCGGATCTC CGCATCGGAA CTGCGAGGGA 
     1081 TCCTGGCCAC TCTGGCTCCG CAGGCCGGGA GCAGAGAAAA CATGAAGGAA CTAAAGGAAC 
     1141 CCAGACAGCG CAAAGATAAT AGGCGTCCAG ATTTGGAAAT CTATAAGCCT GGACTTTCTC 
     1201 GACTAAGGAA CAGACCTAAA ACTAAGGAGG CTTCTGGGAA TGAGGAATTT AAGGATGAGA 
     1261 TAGTAAATGA CAGAGATTCT TCTGCTGTTG GGAATGATAC ACAGCTTATT CAAGTTTGCA 
     1321 AGGAATTGGA CAGCCAACAG CAAAATGGCC CTATAGATGC AGAAAATAGT CAGGCACAGG 
     1381 AAACTTTTCC AAAGACTGTT GGACTAGAGG ACCGAAGTCT AAAGATCATC AAAAGAAGCA 
     1441 AGAAACCAGA CCTGCAGATC TACCAGCCTG GAAGGCGGTT GCAGACCATT ACCAAAGAGT 
     1501 CAGCAGGCAG GGCAGATGAA GAAGAGATCC TCAACCAAGT GGAGCAGTTG AGGATAGAGG 
     1561 AGGATGAGTG TAAGGGAGAA GCTATAAAAG AAGAAGTTAA TAACAAACCG GATAAAACCG 
     1621 AGATAGAAAA GCACCAAAGT AATGACCGTG TAAGAACTGC CAAGGGAGAA AAGGGAAAAA 
     1681 AGATTGAAAA GGGGGAGGGG TCAAAGAAGG TGGCTGATGA CTCTGTCCCA GGAAAGCCTG 
     1741 GCTCTGTAAA GAGATACTCG AGATCAGACA AGAGAAGGAA TCGCTACCGC ACTTGCAGTA 
     1801 CCAGCTCAGC TGGTAGCAAC AACAGTGCTG AGGGAGCTGG CCTGACCGAT AATGGATGTC 
     1861 GCCGGCGGCG CCAGGATCGG GCCAAGGAGA GGCCACGACT GAAGAAGCAG GTATCTTTGT 
     1921 CCTCAACTGA TTCCTTAGAT GAGGACAGAG TTGATGAGCC TGATGTCCTA GGGTCCAGGA 
     1981 GGAGCTCAGA AAGGAAGAAG CATTTAGAAA GAAATTGGTC TGGCTGTGGT GAGGGTGAAC 
     2041 AGAAAAGCAA TGGTAAAGAA AACCGAAGTG CCCTTCGTGT CACTTTTGAT GCAGAAACCA 
     2101 TGAGTAAAGA CTCCCCTGTG GTGAGGTCAG TCAAAGATAA TGTGGATAGA ATGAAGTCTG 
     2161 ACAAAGGCCC AAGCAGTGGG GGTAAGGGCT CTGAGAAGCA GGAGCTCAGA CACCCGAGAC 
     2221 AAGAACTTCG AGATCGTGGT CGTGGTATCC TGATTCTGCC TGCCCACACT GCCCTATCTG 
     2281 TCAGTTCATC AGGTTCTCCG GAGTCTACAC CTTTGGGACC TCGACTTTTA TTTGGATCTG 
     2341 GTAGTAAGGG ATCTCGGAGT TGGGGCCGTG GAGGCACTAC CCGCCGACTA TGGGACCCAA 
     2401 ACAATCCTGA CCAGAAGCCT GCCCTAAAAA GCCAGACACC TCAGCTACAT TTCTTGGACA 
     2461 CTGATGATGA AATTAGCCCT ACATCTTGGG GTGATTCACG TCAGGCCCAA GCATCTTACT 
     2521 ATAAATTTCA AAACTCTGAT AACCCCTATT ATTACCCTCG GACACCAGGC CCTGCCTCTC 
     2581 AGTATCCCTA TGCAGGCTAT AGCCCTCTGC AGTACCCAGT AGGCCCTACA AATGGTATGT 
     2641 ATCCAGGGGC TTACTACCCG GGCTATCCTG CCCCCTCAGG ACAATATGTG TGTAGCCCTC 
     2701 TCCCTGCTAG CACCATGAGT CCTGAGGAAA TAGAACAGCA CGTGCGGAAC ATGCAGCAAC 
     2761 AGGAGCTCCA TCGGCTTCTC CGAGTGGCTG ACAACCAGGA ACTGCAGCTC AGCAACCTGC 
     2821 TTTCCAGGGA CCGCATCAGC ACTGAGGGCA TGGAGAAGAT GGCTCAGCTC AGAACTGAAC 
     2881 TACTGCAGCT GTACGAGCGC TGTATTCTAT TAGATATTGA ATTCTCCGAC AGTCAGAATG 
     2941 TAGATCAGAT CCTGTGGAAG AACGCTTTCT ATCAGGTGAT TGAGAAGTTC AGGCAGCTTC 
     3001 TCAAGGATCC AAACAGTGAG AACCCAGAGC AGATTCGGAA CAGACTCTTG GAGCTCTTAG 
     3061 ATGAGGGCAG TGACTTCTTT GATAGTTTGC TTCAGAAACT ACAGGTTACT TACAAGTTCA 
     3121 AACTGGAGGA CTACATGGAT GGGCTTGCCA TTCGAAGCAA GCCTTTACGA AAAACAGTGA 
     3181 AATATGCCTT GATCAGTGCT CAACGAAGTA TGATTTGCCA AGGAGATATC TCTAGGTATC 
     3241 GGGAGCAAGC CAACGATACA GCAAACTATG GGAAGGCTCG CAGTTGGTAT CTGAAGGCTC 
     3301 AGCACATTGC TCCCAAGAAT GGGCGCCCCT ATAACCAGTT GGCTCTACTG GCAGTGTATA 
     3361 CGAGGAGGAA GCTTGATGCT GTATATTACT ATATGCGCAG TTTAGCAGCC AGCAACCCTA 
     3421 TCCTGACTGC CAAGGAGAGT CTCATGAGTT TATTTGAAGA GACTAAACGA AAGGCTGAAC 
     3481 AGATGGAAAA GAAGCAGCAT GAGGAATTTG ATATGAGCCC TGATAAGTGG CGAAAAGGAA 
     3541 AGAAGTCTAC TTTCCGGCAT GTTGGGGATG ACACTACTCG CCTGGAGATT TGGATTCATC 
     3601 CATCACATTC TCGGTCTGCC CAGGGCACTG AGTCTGGGAA GGATTCTGAG CAGGAGAATG 
     3661 GTCTGGGCAG CCTGAGTCCC AGTGATCTGA ACAAAAGGTT CATCCTCAGT TTTCTCCATG 
     3721 CCCATGGGAA GCTGTTTACC CGGATTGGGA TGGAGACATT CCCTGCAGTG GCTGAGAAGG 
     3781 TCCTTAAGGA GTTCCAAGTA TTGCTGCAGC ATAGCCCATC TCCTATTGGA AGTACCCGTA 
     3841 TGCTGCAGCT TATGACCATC AACATGTTTG CCGTGCATAA CTCCCAGTTG AAAGACTGCT 
     3901 TCTCAGAGGA GTGTCGTTCT GTGATCCAGG AACAAGCTGC ATCCTTGGGC TTGGCTATGT 
     3961 TTTCTCTACT GGTCCAGCGC TGCACATGCC TACTTAAGGA CTCTGCTAAA GCTCAGCTGT 
     4021 CCTCTCCTGA GGACCAAGAG GACCAAGATG ACATCAAGGT ATCTTCCTTC GTCCCGGACC 
     4081 TGAAGGAGCT GCTCCCCAGT GTGAAAGTCT GGTCTGATTG GATGCTTGGC TACCCAGACA 
     4141 CCTGGAATCC TCCACCCACA TCTTTGGATC TGCCCTTGCA GGTTGCTGTG GATGTCTGGT 
     4201 CAACACTAGC TGATTTCTGT AACATACTGA CTGCAGTCAA TCAGTCTGAG GTGCCACTGT 
     4261 ACAAGGACCC AGATGATGAC CTCACCCTGC TTATCCTGGA AGAGGATCGG CTTCTCTCTG 
     4321 GCTTTGTCCC CTTGCTGGCT GCCCCTCAGG ACCCCTGCTA CGTGGAGAAA ACCTCGGATA 
     4381 AGGTTATTGC AGCTGACTGC AAAAGGGTCA CAGTGCTGAA GTATTTTCTG GAAGCCCTTT 
     4441 GTGGACAAGA AGAGCCTCTG CTGGCATTCA AGGGTGGAAA ATATGTGTCA GTGGCACCCG 
     4501 TCCCAGACAC CATGGGAAAG GAAATGGGAA GCCAAGAGGG AAAACAACTG GAAGATGAGG 
     4561 AAGAAGATGT GGTGATTGAA GACTTTGAGG AAGATTCAGA GGCTGAAGGC AGTGGGGGTG 
     4621 AGGATGACAT CAGGGAACTT CGGGCCAAGA AGCTGGCTCT AGCAAGGAAG ATAGCTGAGC 
     4681 AGCAGCGTCG CCAGGAAAAG ATCCAGGCTG TTTTGGAAGA CCAGAGTCAG ATGAGGCAGA 
     4741 TGGAGCTGGA GATCAGACCC TTGTTCCTCG TACCAGATAC CAACGGCTTC ATTGACCACC 
     4801 TGGCCAGTCT GGCTCGGCTG CTGGAGAGCA GGAAGTACAT CCTGGTGGTG CCCCTCATCG 
     4861 TGATCAATGA GCTGGACGGC CTCGCCAAAG GGCAGGAGAC AGACCACCGG GCTGGGGGCT 
     4921 ATGCCCGTGT GGTGCAAGAA AAGGCCCGAA AATCCATTGA GTTCCTCGAA CGGAGATTTG 
     4981 AGAGTCGGGA CTCCTGCCTG CGGGCCTTGA CCAGCCGTGG CAATGAACTT GAATCTATCG 
     5041 CCTTCCGAAG TGAGGACATT ACTGGACAGC TGGGTAACAA CGATGACCTG ATCCTCTCTT 
     5101 GCTGCCTGCA CTACTGCAAA GACAAGGCTA AGGATTACAT GCCCACCAGC AAAGAGGAGC 
     5161 CAATCCGCCT GTTACGGGAG GTGGTGTTGC TGACCGATGA TCGGAACCTT CGTGTAAAGG 
     5221 CGCTGACAAG GAATGTGCCT GTGAGGGACA TCCCAGCCTT CCTTACGTGG GCCCAGGTGG 
     5281 GCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5341 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5401 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5461 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5521 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5581 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5641 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5701 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5761 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5821 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5881 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5941 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     6001 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     6061 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     6121 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6181 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6241 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6301 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6361 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6421 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6481 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6541 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6601 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6661 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6721 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6781 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6841 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6901 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6961 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     7021 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     7081 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     7141 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7201 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7261 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7321 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7381 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7441 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7501 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7561 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7621 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7681 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7741 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7801 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7861 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7921 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7981 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     8041 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     8101 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     8161 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8221 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8281 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8341 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8401 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8461 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8521 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8581 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8641 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8701 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8761 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8821 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8881 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8941 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     9001 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     9061 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     9121 TTTAACGCGA ATTTT
//