LOCUS       MR211888                8733 bp    DNA     circular  SYN 
DEFINITION  Sympk (Myc-DDK-tagged) - Mouse symplekin (cDNA clone MGC:54793 IMAGE:4163760)
ACCESSION   BC049852
VERSION     BC049852.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4890..4981 
                     /label="Myc-DDK"  
     misc_feature    5011..5596 
                     /label="PolyA signal"  
     misc_feature    5068..5087 
                     /label="Restriction Enzyme"  
     misc_feature    5872..6544 
                     /label="ColE1"  
     misc_feature    6974..7768 
                     /label="Kan/Neo"  
     misc_feature    7803..8105 
                     /label="SV40 ori"  
     misc_feature    8191..8668 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4872..4878 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4872 
                     /label="Sympk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAGCAGC AGCGGGGACA GTGTCACCCG CCGGAGTGTG GCATCACAGT 
     1081 TTTTCACACA AGAAGAAGGG CCAAGCATCG ATGGCATGAC CACTTCTGAG AGGGTGGTAG 
     1141 ATCTTCTGAA CCAGGCTGCA TTGATCACCA ATGACTCCAA GATCACAGTG CTCAAACAGG 
     1201 TCCAAGAGCT GATCATCAAC AAGGACCCCA CCCTGCTTGA CAACTTCCTG GATGAGATCA 
     1261 TCGCTTTCCA AGCAGACAAA TCCATTGAAG TGCGCAAGTT TGTCATTGGC TTCATTGAGG 
     1321 AGGCATGCAA GCGGGACATT GAGCTGCTGC TAAAACTGAT TGCCAACCTC AACATGCTAT 
     1381 TGCGGGATGA AAATGTGAAC GTGGTTAAGA AGGCCATCCT CACCATGACC CAGCTCTACA 
     1441 AGGTGGCCCT ACAGTGGATG GTGAAGTCTC GAGTCATCAG TGATCTCCAG GAGGCCTGCT 
     1501 GGGACATGGT GTCCTCCATG GCTGGGGAAA TCATCCTGCT TTTGGATTCT GACAACGATG 
     1561 GCATCCGTAC ACATGCCATC AAGTTTGTGG AGGGCCTCAT TGTCACCCTG TCACCCCGCA 
     1621 TGGCTGACTC AGAGGTCCCA CGACGCCAGG AGCATGACAT CAGTCTGGAC CGCATTCCTC 
     1681 GAGATCACCC CTACATCCAG TACAATGTCC TATGGGAAGA AGGCAAGGCA GCTGTGGAGC 
     1741 AGCTGCTGAA GTTCATGGTG CACCCCGCCA TCTCCTCCAT CAACCTGACC ACAGCGCTCG 
     1801 GCTCCCTTGC CAACATCGCC CGCCAGAGAC CCATGTTCAT GTCCGAGGTG ATCCAGGCCT 
     1861 ATGAGACTCT GCATGCCAAC CTGCCCCCAA CGCTGGCCAA GTCCCAGGTG AGCAGTGTGC 
     1921 GGAAGAACCT TAAGCTGCAC TTGCTCAGTG TGCTCAAGCA CCCTGCCTCC TTGGAGTTCC 
     1981 AGGCCCAGAT CACCACCCTG CTGGTGGATC TGGGCACGCC CCAGGCTGAG ATCGCCCGAA 
     2041 ACATGCCTAG CAGCAAGGAC TCCCGCAAGC GGCCCCGGGA CGATACAGAT TCCACGCTCA 
     2101 AGAAGATGAA GCTGGAGCCC AACTTGGGAG AAGACGATGA GGACAAAGAC TTGGAGCCTG 
     2161 GCCCATCAGG GACGTCAAAG GCGTCAGCCC AGATCTCAGG CCAGTCAGAC ACGGACATCA 
     2221 CTGCTGAGTT CTTGCAGCCT CTGCTGACAC CTGACAATGT GGCCAATCTG GTCCTCATCA 
     2281 GCATGGTGTA CCTGCCTGAG ACCATGCCTG CCTCCTTCCA AGCCATCTAC ACCCCTGTGG 
     2341 AGTCAGCAGG CACTGAAGCC CAGATCAAGC ACCTGGCTCG ACTCATGGCC ACACAGATGA 
     2401 CAGCTGCAGG ACTGGGTCCT GGTGTGGAAC AGACGAAACA GTGCAAGGAG GAGCCCAAGG 
     2461 AGGAGAAGGT GGTAAAGCCG GAGAGCGTCC TGATCAAGCG GCGCCTGTCG GTGCAGGGCC 
     2521 AGGCCATCTC TGTGGTAGGC TCTCAGAGCA CCATGTCTCC CTTGGAGGAG GAGGTACCCC 
     2581 AGGCCAAGAG GAGGCCAGAA CCCATCATCC CTGTCACGCA GCCACGGCTG GCAGGCGCAG 
     2641 GTGGGCGCAA GAAGATCTTC CGTCTGAGCG ATGTGCTGAA GCCCCTGACA GATGCCCAGG 
     2701 TGGAAGCCAT GAAGCTAGGA GCTGTGAAGC GGATCCTGAG GGCTGAGAAG GCTGTAGCCT 
     2761 GCAGTGGGGC AGCCCAGGTG CGCATCAAGA TCCTGGCCAG CCTCGTAACA CAGTTCGACT 
     2821 CCGGATTCAA GGCAGAGGTG CTGTCCTTTA TCCTGGAAGA TGTCCGGGCC CGTCTGGACC 
     2881 TGGCTTTTGC CTGGCTCTAC CAGGAATATA ATGCCTACCT GGCAGCAGGG ACCTCAGGCA 
     2941 CTCTGGACAA ATATGAGGAT TGCCTCATCT GCCTGCTCTC TGGACTACAA GAGAAACCAG 
     3001 ACCAGAAAGA TGGGATCTTT ACCAAGGTGG TTTTGGAGGC CCCACTGATC ACTGAGAGTG 
     3061 CCTTGGAGGT GATACGGAAG TACTGTGAGG ATGAGAGTCG TGCCTACCTG GGCATGTCGA 
     3121 CACTTGGAGA CCTGATCTTC AAGCGCCCTT CTCGCCAGTT CCAGTATCTG CACGTTCTTC 
     3181 TTGACCTCAG CTCTCACGAG AAGGACAGGG TGCGCTCCCA GGCCTTGCTC TTCATTAAGC 
     3241 GAATGTATGA GAAGGAGCAG CTTCGAGAGT ATGTGGAGAA ATTTGCCCTC AACTACCTGC 
     3301 AGCTCCTGGT CCACCCCAAC CCACCCTCGG TGCTGTTTGG AGCCGACAAG GACACAGAGG 
     3361 TGGCCGCACC CTGGACAGAG GAAACAGTGA AGCAGTGTTT GTATCTGTAT CTGGCTCTCC 
     3421 TGCCTCAGAA CCACAAGCTG ATCCATGAGC TGGCAGCCGT GTACACTGAA GCCATCGCTG 
     3481 ACATCAAGCG GACAGTGCTG AGGGTCATTG AGCAGCCGAT CCGAGGGATG GGCATGAACT 
     3541 CTCCAGAGCT GCTGCTGCTG GTAGAAAACT GTCCCAAGGG GGCAGAGACG CTGGTCACCC 
     3601 GCTGCCTGCA CAGCCTCACC GACAAAGTGC CACCATCCCC AGAACTGGTG AAGCGGGTCC 
     3661 GAGACCTGTA CCACAAACGG CTGCCTGATG TGCGCTTCCT CATCCCCGTG CTCAATGGGT 
     3721 TGGAGAAGAA AGAGGTGATC CAGGCGCTGC CCAAGCTCAT CAAACTCAAC CCCATTGTGG 
     3781 TGAAGGAAGT CTTCAACCGC CTGCTAGGCA CTCAGCACGG TGAGGGGAAC TCGGCCCTGT 
     3841 CCCCCCTGAA TCCAGGAGAG CTGCTGATTG CATTGCACAA TATTGACTCT GTGAAGTGTG 
     3901 ACATGAAGTC CATCATCAAA GCCACCAACC TGTGTTTTGC GGAGCGGAAT GTGTACACGT 
     3961 CGGAAGTGCT AGCTGTGGTG ATGCAGCAGC TGATGGAGCA AAGCCCTCTG CCCATGCTGC 
     4021 TCATGCGGAC CGTCATCCAG TCCCTCACCA TGTACCCCCG CCTGGGGGGC TTCGTCATGA 
     4081 ACATCCTGGC CCGACTCATC ATGAAGCAGG TATGGAAGTA CCCCAAGGTA TGGGAGGGCT 
     4141 TCATCAAGTG CTGCCAGCGC ACCAAGCCAC AGAGTTTCCA GGTCATCCTG CAGCTACCTC 
     4201 CACAGCAGCT TGGCGCAGTC TTTGACAAGT GCCCTGAGCT CCGGGAGCCA CTGTTGGCCC 
     4261 ATGTGCGCTC CTTCACTCCC CACCAGCAAG CACACATCCC CAACTCCATC ATGACCATCC 
     4321 TGGAGGCCAC TGGAAAGCAG GAGCCCGAAG TCAAGGAAGC ACCTTCAGGG CCCCTGGAAG 
     4381 AGGATGACCT GGAGCCCTTG GCTCTGGCTT TGGCCCCGGC TCCGGCCCCG GCCCCGGCCC 
     4441 CGGCCCCTGC CCCACGGCCC CCTCAGGACC TCATCGGCCT GCGATTGGCT CAGGAGAAGG 
     4501 CCCTGAAGAG GCAGCTGGAG GAGGAACAGA AGCAGAAGCC CACAGGCATC GGAGCGCCCG 
     4561 CTGCCTGTGT GTCCTCAACG CCCTCGGTGC CAGCAGCAGC CCGAGCTGGG CCCACCCCAG 
     4621 CTGAGGAGGT CATGGAATAC CGGGAGGAGG GTCCCGAATG CGAGACCCCG GCCATCTTCA 
     4681 TCAGCATGGA CGACGACTCG GGGCTGGCTG AAACCACACT GCTGGACTCT AGTCTCGAGG 
     4741 GGCCCCTGCC TAAGGAGGCA GCAGCAGTCG GGTCGAGCTC CAAGGATGAG CGGAGCCCCC 
     4801 AGAACCTCAG CCATGCTGTG GAAGAAGCCT TGAAGACCTC CAGCCCAGAA ACCAGGGAAC 
     4861 CCGAGAGCAA GGGGAACAGC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4921 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4981 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5041 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5101 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5161 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5221 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5281 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5341 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5401 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5461 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5521 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5581 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5641 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5701 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5761 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5821 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5881 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5941 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6001 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6061 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6121 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6181 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6241 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6301 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6361 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6421 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6481 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6541 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6601 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6661 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6721 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6781 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6841 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6901 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6961 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7021 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7081 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7141 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7201 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7261 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7321 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7381 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7441 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7501 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7561 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7621 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7681 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7741 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7801 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7861 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7921 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7981 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8041 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8101 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8161 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8221 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8281 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8341 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8401 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8461 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8521 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8581 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8641 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8701 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//