LOCUS       MR211864                8658 bp    DNA     circular  SYN 
DEFINITION  L1cam (Myc-DDK-tagged) - Mouse L1 cell adhesion molecule (L1cam)
ACCESSION   NM_008478
VERSION     NM_008478.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4815..4906 
                     /label="Myc-DDK"  
     misc_feature    4936..5521 
                     /label="PolyA signal"  
     misc_feature    4993..5012 
                     /label="Restriction Enzyme"  
     misc_feature    5797..6469 
                     /label="ColE1"  
     misc_feature    6899..7693 
                     /label="Kan/Neo"  
     misc_feature    7728..8030 
                     /label="SV40 ori"  
     misc_feature    8116..8593 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4797..4803 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4797 
                     /label="L1cam"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCGTGATG CTGCGGTACG TGTGGCCTCT CCTCCTCTGC AGCCCCTGCC 
     1081 TGCTCATACA GATTCCAGAC GAATATAAAG GACACCATGT GCTAGAGCCA CCTGTCATCA 
     1141 CGGAACAGTC TCCACGGCGC CTGGTTGTCT TCCCAACAGA TGACATAAGC CTGAAATGTG 
     1201 AAGCCAGAGG CAGACCCCAA GTGGAGTTCC GCTGGACGAA AGATGGCATC CACTTCAAAC 
     1261 CCAAGGAAGA ATTGGGTGTA GTGGTGCATG AGGCACCCTA TTCTGGCTCC TTCACCATCG 
     1321 AAGGCAACAA CAGCTTTGCC CAGAGGTTTC AGGGCATCTA TCGCTGCTAT GCCAGCAATA 
     1381 AGCTAGGAAC TGCCATGTCG CATGAGATCC AGCTCGTGGC CGAGGGTGCC CCCAAGTGGC 
     1441 CGAAGGAGAC TGTAAAACCT GTGGAAGTGG AGGAAGGAGA ATCAGTAGTT CTGCCTTGCA 
     1501 ACCCTCCACC CAGTGCAGCC CCACTTAGGA TCTACTGGAT GAACAGCAAG ATTTTGCACA 
     1561 TCAAACAAGA TGAGCGGGTG TCCATGGGCC AGAATGGAGA CCTATATTTT GCCAATGTGC 
     1621 TTACCTCAGA CAATCATTCA GACTACATCT GCAATGCCCA CTTCCCTGGT ACCCGGACCA 
     1681 TCATTCAAAA GGAACCTATT GACCTCCGGG TCAAGCCCAC CAACAGCATG ATTGACCGGA 
     1741 AGCCACGTCT GCTCTTTCCC ACAAACTCCA GCAGCCGCCT GGTAGCCTTG CAGGGCCAGT 
     1801 CATTGATCCT GGAGTGCATT GCTGAGGGAT TCCCTACACC CACCATCAAG TGGCTGCACC 
     1861 CCAGTGACCC AATGCCAACA GACCGTGTTA TCTACCAAAA CCACAACAAG ACCCTGCAAC 
     1921 TACTCAATGT GGGCGAAGAG GACGATGGCG AGTATACCTG CCTTGCTGAG AACTCGCTGG 
     1981 GCAGTGCCCG GCATGCCTAC TATGTTACTG TGGAAGCTGC CCCATATTGG CTGCAGAAGC 
     2041 CCCAGAGCCA TTTGTATGGT CCAGGAGAGA CTGCCCGCCT AGACTGCCAA GTCCAGGGCA 
     2101 GGCCCCAACC AGAGATCACT TGGAGAATCA ACGGAATGTC TATGGAGACG GTGAACAAGG 
     2161 ACCAGAAGTA CCGGATTGAG CAGGGGTCTC TGATCTTGAG TAACGTGCAG CCAAGTGACA 
     2221 CAATGGTGAC CCAGTGTGAA GCCCGCAACC AGCATGGGCT CCTGCTAGCC AATGCCTACA 
     2281 TTTATGTTGT CCAGCTGCCA GCCAGGATCC TAACAAAAGA CAATCAGACA TACATGGCAG 
     2341 TTGAGGGCAG TACTGCTTAC TTGCTGTGCA AAGCCTTTGG AGCTCCTGTT CCCAGTGTCC 
     2401 AGTGGCTGGA TGAAGAAGGA ACCACAGTGC TTCAGGATGA ACGATTTTTC CCCTATGCCA 
     2461 ATGGAACGCT GAGCATCAGA GACCTCCAGG CCAATGACAC TGGACGCTAT TTCTGCCAGG 
     2521 CTGCCAATGA CCAGAACAAT GTGACCATTT TGGCTAACCT ACAGGTTAAA GAAGCAACCC 
     2581 AGATCACACA GGGGCCCCGG AGCGCAATTG AGAAGAAAGG TGCAAGGGTG ACATTCACGT 
     2641 GCCAGGCCTC CTTTGACCCC TCTTTGCAGG CCAGCATCAC TTGGCGTGGA GATGGGAGAG 
     2701 ACCTACAGGA ACGTGGGGAC AGTGACAAGT ATTTCATAGA AGATGGGAAA CTAGTCATCC 
     2761 AGAGCCTGGA CTACAGTGAC CAGGGCAACT ACAGTTGTGT GGCCAGCACT GAACTGGATG 
     2821 AGGTGGAGAG CAGGGCACAG CTCTTAGTGG TGGGGAGCCC TGGGCCAGTG CCTCACCTGG 
     2881 AGCTGTCCGA CCGCCACCTG CTGAAGCAGA GCCAGGTGCA CTTGTCTTGG AGCCCTGCTG 
     2941 AAGACCACAA CTCTCCCATT GAGAAGTATG ACATTGAATT TGAGGACAAG GAAATGGCTC 
     3001 CTGAGAAATG GTTCAGTCTG GGCAAGGTGC CAGGAAATCA GACCTCTACT ACCCTCAAGC 
     3061 TGTCCCCCTA TGTCCACTAC ACCTTTCGGG TCACTGCCAT TAACAAATAT GGTCCTGGAG 
     3121 AACCCAGCCC TGTCTCTGAG ACTGTGGTCA CACCTGAGGC AGCCCCAGAG AAGAACCCTG 
     3181 TGGATGTGAG AGGGGAAGGG AATGAGACCA ACAATATGGT CATCACATGG AAGCCCCTTC 
     3241 GGTGGATGGA TTGGAATGCC CCCCAGATTC AGTACCGTGT ACAGTGGCGT CCACAGGGCA 
     3301 AGCAGGAGAC CTGGAGGGAA CAGACCGTGA GCGACCCTTT CCTGGTGGTG TCTAACACTT 
     3361 CCACATTTGT GCCTTATGAG ATCAAAGTCC AGGCAGTGAA CAACCAGGGC AAGGGCCCTG 
     3421 AGCCCCAGGT CACCATTGGC TATTCAGGGG AAGACTACCC CCAGGTGAGC CCTGAACTTG 
     3481 AAGACATCAC AATCTTCAAC TCAAGTACTG TGCTTGTCAG GTGGAGGCCT GTGGACTTGG 
     3541 CCCAGGTTAA GGGCCACCTC AAGGGATACA ATGTAACATA CTGGTGGAAG GGCAGCCAGA 
     3601 GAAAGCACAG CAAGAGGCAT ATCCACAAAA GCCACATAGT GGTACCTGCA AATACCACCA 
     3661 GTGCCATCCT CAGTGGTTTG CGCCCTTACA GCTCTTACCA TGTGGAGGTA CAGGCCTTTA 
     3721 ATGGGCGGGG CTTGGGGCCT GCGAGTGAAT GGACCTTCAG CACCCCAGAG GGAGTGCCTG 
     3781 GCCACCCTGA GGCATTACAC CTGGAGTGTC AGTCGGACAC TAGTCTGCTA CTGCACTGGC 
     3841 AGCCACCACT CAGCCACAAT GGAGTGCTCA CTGGCTACCT GCTCTCTTAC CATCCCGTGG 
     3901 AAGGGGAAAG CAAAGAGCAG TTGTTCTTCA ACCTTTCGGA CCCAGAACTC CGGACTCATA 
     3961 ATCTGACCAA CCTCAACCCT GATCTACAGT ACCGCTTCCA GCTTCAGGCC ACCACCCAAC 
     4021 AGGGTCCTGG TGAGGCCATC GTGCGTGAAG GAGGCACCAT GGCCCTGTTT GGCAAGCCAG 
     4081 ATTTTGGCAA CATCTCAGCC ACAGCAGGTG AAAACTACAG CGTGGTCTCC TGGGTCCCTC 
     4141 GGAAGGGCCA GTGCAATTTC AGGTTCCATA TCTTGTTCAA AGCCTTACCA GAAGGGAAAG 
     4201 TGAGCCCTGA TCACCAGCCT CAGCCTCAGT ATGTCAGCTA CAATCAGAGC TCCTACACAC 
     4261 AATGGAACCT ACAGCCTGAC ACCAAATATG AGATCCACCT GATAAAGGAG AAGGTCCTCC 
     4321 TGCACCATCT GGATGTGAAG ACTAATGGAA CTGGCCCTGT GCGAGTTTCT ACTACAGGTA 
     4381 GCTTTGCCTC CGAGGGCTGG TTCATCGCCT TTGTCAGCGC TATCATTCTC TTGCTCCTCA 
     4441 TCCTGCTCAT CCTCTGCTTC ATCAAACGCA GCAAGGGTGG CAAATACTCA GTGAAGGACA 
     4501 AGGAGGACAC TCAGGTAGAT TCCGAGGCCC GGCCCATGAA AGACGAGACC TTCGGCGAGT 
     4561 ACAGGTCCCT GGAGAGTGAC AATGAAGAGA AGGCCTTTGG CAGCAGCCAG CCATCTCTCA 
     4621 ACGGAGACAT CAAACCCCTA GGCAGTGATG ACAGCCTGGC TGATTATGGG GGCAGTGTGG 
     4681 ACGTCCAGTT CAATGAGGAT GGCTCTTTCA TCGGCCAGTA CAGTGGCAAG AAAGAGAAGG 
     4741 AGGCAGCAGG AGGCAATGAC AGTTCAGGGG CTACCTCTCC TATCAATCCT GCAGTAGCCC 
     4801 TAGAAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4861 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4921 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4981 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5041 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5101 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5161 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5221 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5281 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5341 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5401 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5461 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5521 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5581 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5641 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5701 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5761 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5821 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5881 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5941 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6001 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6061 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6121 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6181 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6241 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6301 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6361 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6421 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6481 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6541 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6601 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6661 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6721 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6781 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6841 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6901 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6961 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7021 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7081 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7141 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7201 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7261 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7321 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7381 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7441 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7501 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7561 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7621 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7681 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7741 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7801 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7861 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8041 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8101 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8161 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8221 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8281 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8341 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8401 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8461 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8521 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8581 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8641 AAATTTAACG CGAATTTT
//