LOCUS       MR211822                8547 bp    DNA     circular  SYN 
DEFINITION  Wnk4 (Myc-DDK-tagged) - Mouse WNK lysine deficient protein kinase 4 (Wnk4)
ACCESSION   NM_175638
VERSION     NM_175638.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4704..4795 
                     /label="Myc-DDK"  
     misc_feature    4825..5410 
                     /label="PolyA signal"  
     misc_feature    4882..4901 
                     /label="Restriction Enzyme"  
     misc_feature    5686..6358 
                     /label="ColE1"  
     misc_feature    6788..7582 
                     /label="Kan/Neo"  
     misc_feature    7617..7919 
                     /label="SV40 ori"  
     misc_feature    8005..8482 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4686..4692 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4686 
                     /label="Wnk4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTAGCACCT CGAAATACGG AGACTGGGGT CCCCATGTCC CAAACCGAGG 
     1081 CTGACCTGGC TCTGCGGCCA TCTCCGGCTC TCACCTCTAC GGGGCCGACC CGCCTGGGGC 
     1141 CACCTCCTCG CCGAGTGCGC CGCTTCTCTG GGAAGGCTGA GCCTCGGCCG CGCTCTTCGA 
     1201 GACCTAGCCG CCGCAGCTCA GTCGATCTGG GACTGCTGAG CTCCTGGTCT CAACCAGCCT 
     1261 CACTCCTTCC GGAACCCCCG GATCCTCCAG ACTCCGCTGG CCCCACGAGG AGCCCACCTT 
     1321 CAAGCTCTAA AGAACCCCCC GAGGGCACAT GGATGGGGGC AGCTCCCGTG AAGGCTGTGG 
     1381 ACTCTGCATG TCCTGAGCTT ACGGGATCTT CAGGAGGTCC AGGGTCCCGG GAGCCACCGA 
     1441 GGGTTCCTGA TGCTGCAGCC CGGGAACGGC GGCGGGAGCA GGAAGAAAAA GAGGACACAG 
     1501 AAACCCAGGC TGTGGCAACG TCTCCGGACG GCCGATACCT CAAGTTTGAC ATTGAGATTG 
     1561 GAAGAGGTTC CTTCAAGACC GTGTATCGAG GGCTAGACAC CGACACCACG GTAGAGGTGG 
     1621 CCTGGTGTGA GCTGCAGACC CGGAAACTCT CTCGGGCCGA GCGGCAGCGT TTCTCCGAGG 
     1681 AAGTTGAGAT GCTCAAGGGG CTGCAGCACC CCAACATCGT CCGCTTCTAT GACTCCTGGA 
     1741 AGTCGGTGCT GAGGGGCCAG GTTTGCATCG TGCTGGTCAC AGAACTCATG ACCTCGGGCA 
     1801 CGCTCAAGAC GTACCTGAGG CGGTTCCGCG AAATGAAGCC CCGAGTCCTT CAGCGCTGGA 
     1861 GTCGCCAGAT TCTACGGGGA CTTCATTTCC TTCATTCCCG AGTGCCCCCC ATCCTGCACC 
     1921 GAGATCTCAA GTGTGACAAC GTCTTCATTA CTGGCCCTTC AGGCTCTGTC AAAATCGGAG 
     1981 ACCTCGGACT GGCCACGCTC AAGCGCGCCT CCTTTGCCAA GAGCGTCATT GGGACCCCGG 
     2041 AATTCATGGC TCCTGAGATG TACGAGGAAA AGTACGACGA GGCTGTGGAT GTGTACGCCT 
     2101 TTGGCATGTG CATGCTGGAG ATGGCTACGT CTGAGTATCC CTACTCCGAG TGTCAGAATG 
     2161 CAGCACAAAT CTACCGCAAG GTCACTTCGG GCACAAAGCC CAACAGCTTC TACAAGGTGA 
     2221 AGATGCCAGA GGTGAAGGAG ATCATTGAAG GCTGCATCCG CACGGATAAG AACGAGAGGT 
     2281 TCACCATCCA GGATCTCCTG GCCCATGCGT TCTTCCGCGA GGAACGAGGT GTGCACGTGG 
     2341 AGCTGGCAGA GGAAGATGAT GGAGAGAAGC CGGGCCTCAA GCTCTGGCTG CGCATGGAGG 
     2401 ATGCGCGGCG CGGGGGGCGT CCACGAGACA ACCAGGCCAT CGAGTTCCTG TTTCAGCTCG 
     2461 GCCGGGACGC TGCGGAGGAG GTGGCTCAGG AGATGGTAGC CTTGGGCTTA GTCTGTGAAG 
     2521 CTGACTACCA GCCAGTGGCC CGTGCAGTTC GAGAACGGGT TGCTGCTATC CAGCGGAAGC 
     2581 GGGAGAAGCT TCGAAAAGCT AGGGAATTGG AGGTTCTCCC ACCAGACTCA GGACCTCCTC 
     2641 CAGCAACTGT GTCTCTGGCT CCGGGTCCCC CCAGTGCCTT CCCCCCAGAG CCTGAGGAGC 
     2701 CAGAGGCTGA CCAGCATCAA TCCTTCCTCT TCCGCCATGC CAGCTACTCA TCAACTACAT 
     2761 CTGATTGCGA GACTGATGGC TACCTCAGTT CCTCCGGCTT CCTGGATGCC TCAGACCCTG 
     2821 CCCTTCAGCC CCCTGGGGGG TTACCATCCA GCCCCGCTGA ATCCCATCTC TGCTTGCCCT 
     2881 CGGGTTTTGC CTTGTCTATT CCACGGTCTG GCCCTGGCAG TGACTTTTCT CCTGGGGATA 
     2941 GCTACGCCTC AGATGCGGCA TCAGGCCTTA GTGACATGGG AGAAGGGGGG CAAATGAGGA 
     3001 AAAATCCAGT GAAGACTCTT CGACGGAGAC CTCGATCCCG GCTCCGGGTC ACTAGTGTCT 
     3061 CAGACCAGAG CGACAGAGTT GTCGAGTGTC AGCTGCAGAC TCACAACAGC AAGATGGTGA 
     3121 CGTTCCGATT TGATCTGGAT GGGGACAGCC CAGAAGAAAT TGCAGCTGCC ATGGTATACA 
     3181 ATGAGTTCAT CCTGCCCTCG GAGCGAGATG GATTCCTGAG CCGGATCCGG GAGATTATCC 
     3241 AGCGAGTGGA GACCCTGCTG AAGAGAGATG CTGGTCCCCC AGAGGCTGCT GAAGATGCCC 
     3301 TGAGCCCCCA GGAAGAGCCA GCAGCCCTGC CTGCCCTCCC AGGCCCACCC AATGCAGAGC 
     3361 CCCAGAGAAG CATCTCCCCA GAACAGAGGA GCTGGGCAGC CTTCTCCACC TCTCCATCTT 
     3421 CTCCTGGCAC CCCCTTGTCC CCTGGGGCCC CCTTTTCCCC TGGGACCCCT CCTGTCTTCC 
     3481 CATGCCCCAT CTTTCCTATC ACTTCACCCT CATGCTATCC CTGCCCATTC TCCCAGGTCT 
     3541 CTTCAAACCC CTATCCACAG GCCCCCAGTT CCCTGCTTCC CTTATCCTCC AGTGCTTCTC 
     3601 AGGTTCCACT TCCATCTTCC TCTCTTCCCA TCAGCGCTCC CCTCCCATTC TCTCCTAGTT 
     3661 ATCCCCAAGA CCCTCTTAGC CCCACTTCTC TTCCCGTCTG CCCCTCTCCT CCCTCTCTTC 
     3721 CCTCTACCAC AGCAGCCCCT CTCCTCTCTC TGGCTAGTGC CTTCTCTCTG GCTGTGATGA 
     3781 CTGTGGCCCA GTCCCTGCTG TCCCCATCCC CTGGACTTCT TTCTCAGTCT CCTCCAGCCC 
     3841 CTCCAGGTCC TCTGCCTAGC CTGCCCCTTT CCCTTGCTTC TTGTGACCAG GAGAGCCTTT 
     3901 CGGCCCAAAC AGCTGAGACA GAGAATGAGG CTTCCCGTAA TCCTGCTCAG CCACTACTGG 
     3961 GTGATGCTAG ACTGGCACCT ATATCTGAAG AGGGAAAGCC CCAGCTTGTT GGCCGTTTCC 
     4021 AAGTGACTTC ATCTAAGGAA CCAGCAGAGC CTCCCCTGCA ACCAGCATCC CCAACTCTCT 
     4081 CCAGATCCCT AAAGCTGCCA AGCCCTCCGC TGACCTCAGA GAGCTCAGAC ACAGAGGACA 
     4141 GTGCTGCAGG AGGCCCAGAG ACTAGGGAGG CTCTGGCAGA GAGTGACCGT GCAGCCGAAG 
     4201 GCCTGGGGGT TGCGGTCGAT GATGAAAAGG ATGAAGGGAA GGAACCCCTA CTTGGAGGCA 
     4261 GTTCCCCAAT CTTGAGCCAT CCCAGCCCAG TGTGGATGAA CTACTCCTAC AGCAGCCTGT 
     4321 GTCTGAGCAG TGAGGAGTCG GAGAGCAGCG GGGAAGACGA GGAATTCTGG GCTGAGCTGC 
     4381 AGAACCTTCG GCAGAAGCAC TTGTCGGAAG TGGAGGCACT ACAGACACTA CAGAAGAAGG 
     4441 AAATCGAGGA CTTATACAGC CGGCTTGGGA AACAGCCCCC ACCTGGTATT GTAGCTCCAG 
     4501 CTGCTATGCT GTCCTGCCGC CAGCGCCGCC TCTCGAAGGG CAGCTTTCCC ACCTCCCGCC 
     4561 GCAACAGCCT GCAGCGCTCT GATCTCCCTG GTCCTGGCAT CATGCGAAGG AACTCTCTGA 
     4621 GTGGCAGCAG CACCGGCTCC CAGGAGCAGC GGGCAAGCAA GGGGGTGACA TTCGCCGGGG 
     4681 ATATTGGCAG GATGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4741 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4801 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4861 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4921 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4981 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5041 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5101 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5161 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5221 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5281 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5341 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5401 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5461 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5521 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5581 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5641 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5701 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5761 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5821 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5881 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5941 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6001 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6061 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6121 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6181 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6241 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6301 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6361 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6421 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6481 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6541 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6601 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6661 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6721 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6781 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6841 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6901 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6961 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7021 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7081 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7141 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7201 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7261 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7321 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7381 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7441 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7501 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7561 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7621 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7681 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7741 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7801 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7861 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7921 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7981 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8041 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8101 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8161 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8221 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8281 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8341 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8401 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8461 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8521 ATTTAACAAA AATTTAACGC GAATTTT
//