LOCUS       MR211815                8532 bp    DNA     circular  SYN 
DEFINITION  Egf (Myc-DDK-tagged) - Mouse epidermal growth factor (Egf)
ACCESSION   NM_010113
VERSION     NM_010113.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4689..4780 
                     /label="Myc-DDK"  
     misc_feature    4810..5395 
                     /label="PolyA signal"  
     misc_feature    4867..4886 
                     /label="Restriction Enzyme"  
     misc_feature    5671..6343 
                     /label="ColE1"  
     misc_feature    6773..7567 
                     /label="Kan/Neo"  
     misc_feature    7602..7904 
                     /label="SV40 ori"  
     misc_feature    7990..8467 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4671..4677 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4671 
                     /label="Egf"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCTGGGGC CGAAGGCCAA CCTGGCTGTT GCTCGCCTTC CTGCTGGTGT 
     1081 TTTTAAAGAT TAGCATACTC AGCGTCACAG CATGGCAGAC CGGGAACTGT CAGCCAGGTC 
     1141 CTCTCGAGAG AAGCGAGAGA AGCGGGACTT GTGCCGGTCC TGCCCCCTTC CTAGTTTTCT 
     1201 CACAAGGAAA GAGCATCTCT CGGATTGACC CAGATGGAAC AAATCACCAG CAATTGGTGG 
     1261 TGGATGCTGG CATCTCAGCA GACATGGATA TTCATTATAA AAAAGAGAGA CTCTATTGGG 
     1321 TGGATGTAGA AAGACAAGTT TTGCTAAGAG TTTTCCTTAA CGGGACAGGA CTAGAGAAAG 
     1381 TGTGCAATGT AGAGAGGAAG GTGTCTGGGC TGGCCATAGA CTGGATAGAT GATGAAGTTC 
     1441 TCTGGGTAGA CCAACAGAAC GGAGTCATCA CCGTAACAGA TATGACAGGG AAAAATTCCC 
     1501 GAGTTCTTCT AAGTTCCTTA AAACATCCGT CAAATATAGC AGTGGATCCA ATAGAGAGGT 
     1561 TGATGTTTTG GTCTTCAGAG GTGACCGGCA GCCTTCACAG AGCACACCTC AAAGGTGTTG 
     1621 ATGTAAAAAC ACTGCTGGAG ACAGGGGGAA TATCGGTGCT GACTCTGGAT GTCCTGGACA 
     1681 AACGGCTCTT CTGGGTTCAG GACAGTGGCG AAGGAAGCCA CGCTTACATT CATTCCTGTG 
     1741 ATTATGAGGG TGGCTCCGTC CGTCTTATCA GGCATCAAGC ACGGCACAGT TTGTCTTCAA 
     1801 TGGCCTTTTT TGGTGATCGG ATCTTCTACT CAGTGTTGAA AAGCAAGGCG ATTTGGATAG 
     1861 CCAACAAACA CACGGGGAAG GACACGGTCA GGATTAACCT CCATCCATCC TTTGTGACAC 
     1921 CTGGAAAACT GATGGTAGTA CACCCTCGTG CACAGCCCAG GACAGAGGAC GCTGCTAAGG 
     1981 ATCCTGACCC CGAACTTCTC AAACAGAGGG GAAGACCATG CCGCTTCGGT CTCTGTGAGC 
     2041 GAGACCCCAA GTCCCACTCG AGCGCATGCG CTGAGGGCTA CACGTTAAGC CGAGACCGGA 
     2101 AGTACTGCGA AGATGTCAAT GAATGTGCCA CTCAGAATCA CGGCTGTACT CTTGGGTGTG 
     2161 AAAACACCCC TGGATCCTAT CACTGCACAT GCCCCACAGG ATTTGTTCTG CTTCCTGATG 
     2221 GGAAACAATG TCACGAACTT GTTTCCTGCC CAGGCAACGT ATCAAAGTGC AGTCATGGCT 
     2281 GTGTCCTGAC ATCAGATGGT CCCCGGTGCA TCTGTCCTGC AGGTTCAGTG CTTGGGAGAG 
     2341 ATGGGAAGAC TTGCACTGGT TGTTCATCGC CTGACAATGG TGGATGCAGC CAGATCTGTC 
     2401 TTCCTCTCAG GCCAGGATCC TGGGAATGTG ATTGCTTTCC TGGGTATGAC CTACAGTCAG 
     2461 ACCGAAAGAG CTGTGCAGCT TCAGGACCAC AGCCACTTTT ACTGTTTGCA AATTCCCAGG 
     2521 ACATCCGACA CATGCATTTT GATGGAACAG ACTACAAAGT TCTGCTCAGC CGGCAGATGG 
     2581 GAATGGTTTT TGCCTTGGAT TATGACCCTG TGGAAAGCAA GATATATTTT GCACAGACAG 
     2641 CCCTGAAGTG GATAGAGAGG GCTAATATGG ATGGGTCCCA GCGAGAAAGA CTGATCACAG 
     2701 AAGGAGTAGA TACGCTTGAA GGTCTTGCCC TGGACTGGAT TGGCCGGAGA ATCTACTGGA 
     2761 CAGACAGTGG GAAGTCTGTT GTTGGAGGGA GCGATCTGAG CGGGAAGCAT CATCGAATAA 
     2821 TCATCCAGGA GAGAATCTCG AGGCCGCGAG GAATAGCTGT GCATCCAAGG GCCAGGAGAC 
     2881 TGTTCTGGAC GGACGTAGGG ATGTCTCCAC GGATTGAAAG CGCTTCCCTT CAAGGTTCCG 
     2941 ACCGGGTGCT GATAGCCAGC TCCAATCTAC TGGAACCCAG TGGAATCACG ATTGACTACT 
     3001 TAACAGACAC TTTGTACTGG TGTGACACCA AGAGGTCTGT GATTGAAATG GCCAATCTGG 
     3061 ATGGCTCCAA ACGCCGAAGA CTTATCCAGA ACGACGTAGG TCACCCCTTC TCTCTAGCCG 
     3121 TGTTTGAGGA TCACCTGTGG GTCTCGGATT GGGCTATCCC ATCGGTAATA AGGGTGAACA 
     3181 AGAGGACTGG CCAAAACAGG GTACGTCTTC AAGGCAGCAT GCTGAAGCCC TCGTCACTGG 
     3241 TTGTGGTCCA TCCATTGGCA AAACCAGGTG CAGATCCCTG CTTATACAGG AATGGAGGCT 
     3301 GTGAACACAT CTGCCAAGAG AGCCTGGGCA CAGCTCGGTG TTTGTGTCGT GAAGGTTTTG 
     3361 TGAAGGCCTG GGATGGGAAA ATGTGTCTCC CTCAGGATTA TCCAATCCTG TCAGGTGAAA 
     3421 ATGCTGATCT TAGTAAAGAG GTGACATCAC TGAGCAACTC CACTCAGGCT GAAGTACCAG 
     3481 ACGATGATGG GACAGAATCT TCCACACTAG TGGCTGAAAT CATGGTGTCA GGCATGAACT 
     3541 ATGAAGATGA CTGTGGTCCC GGGGGGTGTG GAAGCCATGC TCGATGCGTT TCAGACGGAG 
     3601 AGACTGCTGA GTGTCAGTGT CTGAAAGGGT TTGCCAGGGA TGGAAACCTG TGTTCTGATA 
     3661 TAGATGAGTG TGTGCTGGCT AGATCGGACT GCCCCAGCAC CTCGTCCAGG TGCATCAACA 
     3721 CTGAAGGTGG CTACGTCTGC AGATGCTCAG AAGGCTACGA AGGAGACGGG ATCTCCTGTT 
     3781 TCGATATTGA CGAGTGCCAG CGGGGGGCGC ACAACTGCGC TGAGAATGCC GCCTGCACCA 
     3841 ACACGGAGGG AGGCTACAAC TGCACCTGCG CAGGCCGCCC ATCCTCGCCC GGACGGAGTT 
     3901 GCCCTGACTC TACCGCACCC TCTCTCCTTG GGGAAGATGG CCACCATTTG GACCGAAATA 
     3961 GTTATCCAGG ATGCCCATCC TCATATGATG GATACTGCCT CAATGGTGGC GTGTGCATGC 
     4021 ATATTGAATC ACTGGACAGC TACACATGCA ACTGTGTTAT TGGCTATTCT GGGGATCGAT 
     4081 GTCAGACTCG AGACCTACGA TGGTGGGAGC TGCGTCATGC TGGCTACGGG CAGAAGCATG 
     4141 ACATCATGGT GGTGGCTGTC TGCATGGTGG CACTGGTCCT GCTGCTCCTC TTGGGGATGT 
     4201 GGGGGACTTA CTACTACAGG ACTCGGAAGC AGCTATCAAA CCCCCCAAAG AACCCTTGTG 
     4261 ATGAGCCAAG CGGAAGTGTG AGCAGCAGCG GGCCCGACAG CAGCAGCGGG GCAGCTGTGG 
     4321 CTTCTTGTCC CCAACCTTGG TTTGTGGTCC TAGAGAAACA CCAAGACCCC AAGAATGGGA 
     4381 GTCTGCCTGC GGATGGTACG AATGGTGCAG TAGTAGATGC TGGCCTGTCT CCCTCCCTGC 
     4441 AGCTCGGGTC AGTGCATCTG ACTTCATGGA GACAGAAGCC CCACATAGAT GGAATGGGCA 
     4501 CAGGGCAAAG CTGCTGGATT CCACCATCAA GTGACAGAGG ACCCCAGGAA ATAGAGGGAA 
     4561 ACTCCCACCT ACCCTCCTAC AGACCTGTGG GGCCGGAGAA GCTGCATTCT CTCCAGTCAG 
     4621 CTAATGGATC GTGTCACGAA AGGGCTCCAG ACCTGCCACG GCAGACAGAG CCAGTTCAGA 
     4681 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4741 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4801 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4861 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4921 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4981 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5041 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5101 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5161 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5221 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5281 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5341 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5401 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5461 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5521 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5581 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5641 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5701 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5761 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5821 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5881 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5941 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6001 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6061 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6121 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6181 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6241 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6301 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6361 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6421 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6481 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6541 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6601 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6661 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6721 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6781 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6841 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6901 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6961 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7021 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7081 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7141 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7201 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7261 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7321 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7381 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7441 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7501 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7561 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7621 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7681 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7741 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7801 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7861 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7921 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7981 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8041 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8101 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8161 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8221 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8281 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8341 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8401 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8461 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8521 AACGCGAATT TT
//