LOCUS       MR211814                8532 bp    DNA     circular  SYN 
DEFINITION  Smc3 (Myc-DDK-tagged) - Mouse structural maintenace of chromosomes 3 (Smc3)
ACCESSION   NM_007790
VERSION     NM_007790.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4689..4780 
                     /label="Myc-DDK"  
     misc_feature    4810..5395 
                     /label="PolyA signal"  
     misc_feature    4867..4886 
                     /label="Restriction Enzyme"  
     misc_feature    5671..6343 
                     /label="ColE1"  
     misc_feature    6773..7567 
                     /label="Kan/Neo"  
     misc_feature    7602..7904 
                     /label="SV40 ori"  
     misc_feature    7990..8467 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4671..4677 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4671 
                     /label="Smc3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACATCAAG CAGGTGATCA TCCAGGGCTT CCGAAGTTAC CGAGACCAAA 
     1081 CAATTGTAGA TCCCTTCAGC TCCAAACATA ATGTCATCGT GGGCAGAAAT GGCTCTGGAA 
     1141 AAAGCAACTT CTTTTATGCA ATCCAGTTTG TTCTCAGTGA TGAATTCAGC CATCTTCGTC 
     1201 CAGAGCAGCG ATTGGCTTTG TTGCATGAGG GTACAGGTCC TCGTGTTATT TCTGCTTTTG 
     1261 TGGAAATCAT TTTTGACAAT TCGGACAACC GGTTACCAAT TGATAAAGAG GAAGTTTCAC 
     1321 TTCGAAGAGT TATTGGTGCC AAAAAGGATC AGTATTTCCT AGATAAGAAG ATGGTCACGA 
     1381 AAAATGATGT GATGAATCTC CTTGAAAGTG CTGGGTTTTC CAGAAGTAAT CCTTATTACA 
     1441 TTGTTAAACA AGGAAAGATC AACCAAATGG CAACGGCACC AGATTCTCAG AGATTAAAGC 
     1501 TGTTGAGAGA AGTAGCTGGT ACTAGAGTGT ATGATGAACG GAAAGAAGAA AGCATCTCCC 
     1561 TGATGAAAGA AACAGAGGGC AAACGGGAAA AGATCAATGA GTTGTTGAAG TACATTGAAG 
     1621 AGCGCCTGCA CACTCTAGAG GAGGAGAAGG AGGAGCTGGC CCAGTATCAG AAGTGGGATA 
     1681 AGATGAGGCG CGCCCTGGAG TACACCATCT ACAACCAGGA GCTCAACGAG ACGCGCGCTA 
     1741 AGCTCGACGA GCTTTCTGCT AAGCGAGAAA CGAGTGGAGA GAAATCCAGA CAACTAAGGG 
     1801 ATGCCCAGCA GGATGCAAGA GACAAAATGG AGGATATTGA GCGCCAGGTT AGAGAACTGA 
     1861 AAACAAAAAT TTCAGCCATG AAAGAAGAAA AAGAACAGCT TAGTGCTGAA AGACAAGAGC 
     1921 AGATTAAGCA AAGGACCAAG TTGGAGCTTA AAGCCAAGGA TTTACAAGAT GAGCTGGCGG 
     1981 GCAACAGTGA ACAGCGGAAA CGTTTATTAA AAGAGAGGCA GAAGCTGCTT GAAAAAATAG 
     2041 AAGAAAAGCA GAAAGAACTG GCAGAAACAG AACCTAAATT CAACAGCGTA AAAGAAAAAG 
     2101 AAGAGCGAGG AATTGCGAGG TTGGCTCAAG CTACACAGGA AAGAACTGAT CTTTATGCAA 
     2161 AGCAGGGTCG AGGAAGCCAG TTTACGTCAA AAGAAGAAAG GGATAAATGG ATTAAAAAGG 
     2221 AACTGAAGTC TTTAGATCAG GCAATTAATG ATAAGAAAAG ACAGATTGCT GCTATTCACA 
     2281 AGGATTTGGA GGATACCGAG GCAAATAAAG AGAAAAATCT GGAGCAATAT AATAAATTGG 
     2341 ATCAGGATCT CAATGAAGTC AAAGCTCGAG TTGAAGAACT GGACAGAAAA TATTATGAAG 
     2401 TAAAAAATAA GAAAGATGAA CTACAAAGTG AAAGAAATTA CTTGTGGAGA GAGGAGAATG 
     2461 CAGAACAACA AGCACTTGCT GCTAAAAGAG AAGACCTTGA GAAGAAGCAG CAACTTCTTA 
     2521 GAGCGGCAAC AGGAAAGGCC ATTTTAAATG GAATAGATAG CATTAACAAA GTGCTAGAAC 
     2581 ATTTTCGGCG AAAAGGTATA AACCAGCATG TTCAAAATGG CTACCATGGC ATCGTAATGA 
     2641 ATAACTTTGA GTGTGAACCA GCTTTCTATA CTTGTGTGGA AGTCACTGCT GGTAACAGGT 
     2701 TATTTTATCA CATTGTTGAT TCAGATGAAG TCAGCACGAA AATTTTAATG GAGTTCAATA 
     2761 AAATGAATCT TCCTGGAGAG GTGACTTTCC TGCCTCTTAA CAAGTTAGAT GTGAGAGATA 
     2821 CTGCCTATCC TGAAACTAAC GATGCTATTC CTATGATCAG TAAGCTGAGG TACAATCCCA 
     2881 GATTTGACAA AGCTTTCAAA CATGTATTTG GAAAGACACT CATCTGTCGG AGCATGGAAG 
     2941 TTTCAACTCA GCTGGCCCGG GCCTTCACTA TGGACTGTAT TACTTTGGAA GGTGATCAAG 
     3001 TCAGCCATCG AGGTGCTCTG ACTGGAGGTT ATTACGACAC AAGAAAGTCT CGACTTGAGT 
     3061 TACAGAAAGA CGTTAGGAAG GCAGAGGAGG AGCTGGGTGA GCTGGAGGCT AAGCTCAATG 
     3121 AAAACCTACG CAGGAACATT GAAAGGATTA ATAATGAAAT TGACCAGTTG ATGAACCAAA 
     3181 TGCAGCAGAT AGAGACCCAA CAAAGAAAAT TTAAAGCATC CAGAGATAGC ATATTATCAG 
     3241 AGATGAAGAT GCTAAAAGAG AAGAGACAGC AATCAGAAAA GACCTTCATG CCAAAGCAAC 
     3301 GTAGCTTACA AAGCTTGGAG GCAAGTCTGC ATGCTATGGA GTCCACCAGA GAGTCACTGA 
     3361 AAGCGGAGCT AGGAACGGAT TTGCTTTCTC AACTCAGTCT GGAAGATCAG AAAAGAGTCG 
     3421 ACGCTCTGAA TGATGAAATC CGTCAGCTGC AGCAGGAAAA CAGACAGCTG CTAAATGAAA 
     3481 GAATTAAACT AGAAGGCATT ATTACTCGAG TAGAGACTTA CCTGAATGAG AATCTGAGGA 
     3541 AACGCTTGGA CCAAGTAGAA CAGGAACTTA ATGAACTGAG AGAGACAGAA GGTGGTACTG 
     3601 TTCTTACTGC CACAACATCA GAACTTGAAG CTATTAATAA AAGAGTAAAA GATACTATGG 
     3661 CAAGATCAGA AGATTTGGAC AATTCCATTG ACAAAACAGA AGCTGGAATT AAAGAGCTCC 
     3721 AGAAAAGTAT GGAGCGCTGG AAAAATATGG AGAAAGAACA CATGGATGCC ATAAATCATG 
     3781 ATACTAAAGA GCTGGAGAAG ATGACCAACC GGCAAGGCAT GCTGTTGAAG AAGAAGGAGG 
     3841 AGTGTATGAA GAAGATCCGG GAGCTGGGGT CCCTCCCCCA GGAAGCGTTT GAGAAGTACC 
     3901 AGACACTGAG CCTGAAGCAG TTGTTTCGAA AACTGGAGCA ATGCAACACA GAGTTAAAGA 
     3961 AGTACAGCCA CGTGAACAAA AAGGCTCTAG ATCAGTTTGT GAATTTCTCT GAGCAGAAGG 
     4021 AAAAGCTGAT AAAGCGGCAA GAGGAATTGG ATAGAGGCTA CAAATCAATC ATGGAATTGA 
     4081 TGAATGTACT TGAACTTAGA AAATATGAAG CTATTCAGTT AACTTTCAAA CAGGTATCTA 
     4141 AGAACTTCAG TGAAGTTTTC CAGAAGTTGG TACCTGGTGG CAAAGCGACT TTGGTGATGA 
     4201 AGAAAGGAGA TGTGGAGGGC AGCCAGTCTC AGGACGAAGG AGAAGGGAGT GGCGAGAGTG 
     4261 AGCGGGGCTC TGGGTCACAG AGCAGTGTTC CATCAGTTGA CCAGTTCACA GGAGTTGGGA 
     4321 TCAGGGTGTC ATTTACAGGG AAGCAAGGGG AGATGAGAGA AATGCAGCAG CTCTCAGGAG 
     4381 GACAGAAATC TCTGGTAGCC CTTGCTCTCA TCTTTGCCAT TCAGAAATGC GACCCTGCTC 
     4441 CTTTTTACCT GTTTGATGAG ATCGACCAAG CTTTGGATGC TCAGCACAGA AAAGCCGTGT 
     4501 CAGATATGAT TATGGAGCTA GCCGTGCATG CCCAGTTTAT TACCACTACT TTCAGGCCTG 
     4561 AACTGCTTGA GTCAGCTGAC AAATTCTATG GTGTAAAGTT CAGAAATAAG GTTAGTCACA 
     4621 TTGATGTGAT CACAGCAGAG ATGGCCAAAG ATTTTGTAGA AGACGATACC ACGCATGGTA 
     4681 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4741 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4801 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4861 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4921 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4981 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5041 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5101 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5161 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5221 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5281 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5341 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5401 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5461 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5521 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5581 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5641 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5701 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5761 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5821 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5881 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5941 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6001 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6061 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6121 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6181 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6241 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6301 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6361 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6421 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6481 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6541 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6601 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6661 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6721 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6781 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6841 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6901 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6961 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7021 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7081 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7141 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7201 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7261 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7321 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7381 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7441 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7501 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7561 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7621 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7681 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7741 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7801 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7861 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7921 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7981 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8041 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8101 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8161 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8221 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8281 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8341 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8401 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8461 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8521 AACGCGAATT TT
//