LOCUS       MR211760                8415 bp    DNA     circular  SYN 
DEFINITION  Itga2 (Myc-DDK-tagged) - Mouse integrin alpha 2 (Itga2)
ACCESSION   NM_008396
VERSION     NM_008396.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4572..4663 
                     /label="Myc-DDK"  
     misc_feature    4693..5278 
                     /label="PolyA signal"  
     misc_feature    4750..4769 
                     /label="Restriction Enzyme"  
     misc_feature    5554..6226 
                     /label="ColE1"  
     misc_feature    6656..7450 
                     /label="Kan/Neo"  
     misc_feature    7485..7787 
                     /label="SV40 ori"  
     misc_feature    7873..8350 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4554..4560 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4554 
                     /label="Itga2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGACCGGGA CAGGCAGGGG GCGCGCTCCT GCTGCGGCTG CTAATGCTAG 
     1081 TTCAAGGTAT TTTGAATTGT CTGGCGTATA ATGTTGGCCT CCCAGGAGCG AAAATATTTT 
     1141 CCGGGCCTTC AAGTGAACAG TTTGGGTATT CAGTACAGCA GCTTACGAAC CCACAAGGCA 
     1201 ACTGGCTACT GGTTGGTTCA CCGTGGAGTG GCTTCCCTGA GAACCGAATG GGAGATGTGT 
     1261 ACAAGTGCCC TGTGGACCTA CCCACTGCCA CCTGTGAAAA GCTGAATCTG CAAAATTCAG 
     1321 CAAGCATCTC AAATGTTACC GAGATAAAGA CCAACATGAG CCTGGGATTG ACCCTCACCA 
     1381 GGAATCCGGG GACCGGAGGC TTTCTAACCT GTGGTCCTCT CTGGGCACAT CAGTGTGGAA 
     1441 ATCAGTATTA TGCAACTGGG ATCTGTTCTG ATGTTAGTCC AGACTTTCAG TTCTTGACCA 
     1501 GCTTTTCACC TGCAGTTCAG GCTTGCCCTT CCCTCGTGGA TGTTGTAGTT GTATGTGATG 
     1561 AATCAAACAG TATTTATCCT TGGGAAGCAG TAAAGAACTT TTTGGTAAAG TTTGTGACAG 
     1621 GCCTGGATAT AGGACCTAAA AAGACACAGG TGGCGTTAAT TCAATATGCC AATGAGCCGA 
     1681 GAATTATATT TAACTTGAAC GATTTCGAAA CCAAAGAGGA TATGGTCCAG GCCACATCTG 
     1741 AGACGCGCCA ACATGGTGGG GACCTCACAA ACACCTTCAG AGCTATCGAA TTCGCAAGAG 
     1801 ACTACGCTTA TTCACAGACT TCTGGCGGGC GCCCGGGTGC TACAAAAGTC ATGGTAGTTG 
     1861 TGACCGATGG CGAGTCCCAT GATGGGTCGA AGCTGAAAAC TGTGATCCAG CAATGCAATG 
     1921 ATGACGAGAT ACTGAGGTTC GGCATAGCAG TTCTTGGGTA TTTAAACAGA AATGCTCTTG 
     1981 ATACTAAAAA TTTAATCAAA GAAATAAAAG CAATTGCTAG TACTCCAACC GAGAGATACT 
     2041 TTTTCAATGT GGCCGACGAA GCGGCTCTTC TGGAGAAGGC TGGAACTCTA GGGGAGCAAA 
     2101 TATTCAGCAT TGAAGGCACT GTTCAAGGAG GAGACAACTT CCAGATGGAA ATGGCACAAG 
     2161 TTGGATTCAG TGCAGATTAC GCTCCTCAAA ATGATATTCT GATGTTAGGT GCAGTGGGGG 
     2221 CTTTTGACTG GAGTGGAACT CTTGTCCAGG AGACATCTCA CAAGCCCGTG ATCTTTCCTA 
     2281 AACAAGCCTT TGACCAGGTT CTGCAGGATA GAAACCACAG TTCGTTTTTA GGTTACTCTG 
     2341 TGGCTGCAAT TTCTACTGAA GACGGTGTCC ATTTTGTTGC TGGTGCTCCT CGAGCAAATT 
     2401 ATACTGGCCA GATAGTGTTG TACAGCGTTA ACAAGCAAGG CAATGTGACC GTGATTCAGT 
     2461 CTCACAGAGG GGACCAGATT GGCTCCTATT TTGGCAGTGT GCTGTGCTCA GTTGATGTAG 
     2521 ATAAAGACAC CATTACAGAC GTGCTCCTGG TAGGTGCTCC TACATACATG AACGACCTCA 
     2581 AGAAAGAAGA GGGAAAAGTC TACCTATTTA CCATCACAAA GGGCATCTTG AATCAGCACC 
     2641 AGTTTCTTGA AGGGCCAGAA GGCACTGGAA ATGCTCGGTT TGGTTCAGCA ATTGCAGCCC 
     2701 TGTCAGACAT CAACATGGAT GGCTTTAATG ATGTGATTGT CGGTTCACCT GTAGAGAATG 
     2761 AGAACTCTGG AGCTGTGTAC ATTTACAATG GTCACCAGGG TACCATTCGC ACCAAGTACT 
     2821 CCCAGAAAAT CCTTGGGTCC AATGGAGCCT TTAGGAGACA TCTCCAGTTC TTTGGAAGGT 
     2881 CCCTGGATGG CTATGGTGAT TTAAATGGGG ATTCCATCAC TGATGTATCC ATTGGTGCTT 
     2941 TGGGGCAAGT GATTCAACTC TGGTCACAAA GCATTGCTGA CGTGGCCATA GAAGCTTTGT 
     3001 TCACTCCAGA CAAAATCACT TTGCTCAACA AGGATGCTAA GATAACCCTA AAGCTCTGCT 
     3061 TCAGAGCAGA GTTTAGACCT GCTGGGCAAA ACAATCAAGT AGCCATCCTG TTTAACATGA 
     3121 CGCTTGATGC GGACGGACAT TCATCCAGGG TAACCTCCAG GGGGGTGTTC AGAGAAAACA 
     3181 GTGAGCGATT CTTGCAGAAG AATATGGTGG TAAACGAAGT GCAGAAGTGT TCTGAGCACC 
     3241 ACATTAGCAT ACAGAAGCCC TCTGATGTTG TCAACCCTTT GGATCTGCGT GTGGACATCA 
     3301 GTTTGGAAAA CCCAGGCACT AGCCCTGCCC TCGAAGCCTA TTCTGAAACT GTCAAAGTCT 
     3361 TCAGTATCCC TTTCTATAAG GAGTGTGGCA GCGATGGAAT TTGCATCTCT GACCTGATCC 
     3421 TGGATGTCCA ACAGCTGCCA GCCATTCAAA CTCAATCCTT TATTGTCAGT AACCAAAACA 
     3481 AGAGGTTAAC ATTTTCAGTC ATCCTGAAAA ACAGAGGGGA AAGTGCATAC AACACTGTGG 
     3541 TCCTGGCCGA GTTTTCTGAG AACTTGTTTT TTGCTTCTTT CTCCATGCCG GTCGATGGAA 
     3601 CAGAAGTAAC ATGTGAGGTT GGTTCATCGC AGAAATCTGT TACCTGTGAT GTGGGATACC 
     3661 CTGCCTTAAA GAGTGAACAG CAGGTGACTT TTACCATTAA CTTCGACTTT AATCTTCAAA 
     3721 ACCTCCAGAA CCAGGCGGCC ATCAATTTCC AAGCCTTCAG TGAGAGCCAA GAAACAAACA 
     3781 AAGCAGACAA TTCGGTCAGT CTCACGATTC CTCTCCTGTA TGATGCCGAA CTTCACTTAA 
     3841 CCCGATCCAC CAACATCAAC TTCTATGAGA TCTCTTCGGA TGAGAACGCT CCTTCTGTCA 
     3901 TCAAGAGTGT CGAAGATATT GGTCCTAAAT TCATCTTCTC CCTCAAGGTA ACAGCCGGAA 
     3961 GTGCCCCAGT GAGCATGGCA TTGGTGACTA TCCACATTCC TCAGTACACC AAGGAAAAGA 
     4021 ACCCACTCCT GTATCTGACT GGGATACAAA CAGACCAGGC TGGCGACATC AGCTGCACAG 
     4081 CAGAAATAAA TCCACTCAAG CTACCACACA CCGCCCCTTC TGTATCTTTC AAGAATGAAA 
     4141 ACTTCCGGCA TACGAAAGAA TTGGACTGCA GAACCACTTC CTGCAGTAAC ATCACCTGCT 
     4201 GGCTGAAAGA CCTTCACATG AAAGCAGAGT ACTTCATCAA TGTGACAACC AGAGTTTGGA 
     4261 ACAGGACCTT TGCTGCTTCG ACTTTCCAGA CTGTGCAGCT GACAGCAGCG GCAGAGATCG 
     4321 ATACACATAA CCCTCAGCTC TTCGTGATTG AGGAAAATGC TGTCACGATT CCCCTCATGA 
     4381 TAATGAAACC CACGGAGAAA GCAGAAGTAC CGACAGGGGT TATCATAGGC AGCATAATTG 
     4441 CGGGAATCCT TTTGCTGTTG GCTATGACTG CAGGTTTGTG GAAGCTCGGC TTCTTCAAAA 
     4501 GACAATATAA GAAAATGGGC CAAAATCCAG ATGAGATGGA TGAGACCACA GAACTCAACA 
     4561 GCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4621 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4681 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4741 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4801 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4861 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4921 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4981 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5041 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5101 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5161 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5221 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5281 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5341 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5401 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5461 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5521 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5581 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5641 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5701 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5761 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5821 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5881 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5941 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6001 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6061 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6121 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6181 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6241 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6301 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6361 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6421 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6481 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6541 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6601 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6661 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6721 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6781 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6841 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6901 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6961 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7021 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7081 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7141 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7201 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7261 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7321 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7381 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7441 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7501 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7561 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7621 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7681 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7741 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7801 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7861 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7921 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7981 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8041 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8101 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8161 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8221 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8281 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8341 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8401 TTTAACGCGA ATTTT
//