LOCUS       MR211727                8364 bp    DNA     circular  SYN 
DEFINITION  Nrd1 (Myc-DDK-tagged) - Mouse nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1)
ACCESSION   NM_146150
VERSION     NM_146150.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4521..4612 
                     /label="Myc-DDK"  
     misc_feature    4642..5227 
                     /label="PolyA signal"  
     misc_feature    4699..4718 
                     /label="Restriction Enzyme"  
     misc_feature    5503..6175 
                     /label="ColE1"  
     misc_feature    6605..7399 
                     /label="Kan/Neo"  
     misc_feature    7434..7736 
                     /label="SV40 ori"  
     misc_feature    7822..8299 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4503..4509 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4503 
                     /label="Nrd1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGAGGAGA GTCGCGGTTG CTGCAGTCTG TGTCACCGGG AGGAAGTTGC 
     1081 GGTGTGAGGC CGGGCGGGAG CTCACGGCTC TGGGGCGAAT CGAAGCACGG GGTCTGTGCG 
     1141 AAGAATCATC GAAACCCTTT CCTACGCTGA CCATGCCTGG AAGAAACAAG GCGAAGTCCA 
     1201 CCTGCAGTTG CCCGGACCTG CAGCCCAATG GACAGGACTT GGGCGAGAGC GGCCGGCTTG 
     1261 CTCGTCTAGG AGCGGATGAA TCTGAGGAGG AGGGACGGTC TTTCAGTAAT GTCGGGGACC 
     1321 CTGAGATCAT CAAGTCTCCC AGCGATCCCA AGCAGTACCG ATACATCAAA TTACAGAATG 
     1381 GCTTGCAGGC ACTTTTGATT TCAGATCTAA GTAATGTGGA AGGTAAGACA GGAAATGCAA 
     1441 CAGATGAAGA GGAGGAAGAA GAGGAGGAGG AAGAGGAAGA AGATGATGAT GATGACGACG 
     1501 ACGACGACGA TGATGATGAA GACTCTGGAG CTGAGATACA AGATGATGAT GAGGAAGGTT 
     1561 TTGATGATGA AGAGGAATTT GATGATGACG ATGATGATGA ACATGATGAT GATGATCTTG 
     1621 AGAATGAGGA AAACGAACTG GAAGAGTTGG AAGAGAGGGT AGAAGCCAGG AAGAAAACTA 
     1681 CTGAGAAACA GTCTGCAGCG GCTCTGTGTG TTGGAGTTGG GAGCTTTGCT GATCCAGATG 
     1741 ACCTGCCTGG GCTGGCACAC TTTTTGGAGC ACATGGTATT CATGGGTAGT TTGAAATACC 
     1801 CTGATGAGAA TGGATTTGAT GCCTTCCTCA AGAAACATGG AGGTAGTGAT AATGCCTCAA 
     1861 CTGATTGTGA ACGCACAGTC TTTCAGTTTG ATGTCCAGAG AAAGTATTTC AAGGAAGCCC 
     1921 TGGATAGATG GGCCCAGTTC TTTATCCATC CACTGATGAT CAGAGATGCA ATTGACCGAG 
     1981 AGGTGGAAGC TGTGGACAGT GAGTATCAGC TTGCAAGGCC TTCTGATGCA AACAGAAAAG 
     2041 AAATGTTGTT TGGAAGCCTC GCTAGACCGG GACATCCTAT GGGGAAGTTT TTTTGGGGAA 
     2101 ATGCTGAGAC ACTCAAGCAT GAACCAAAGA AGAATAACAT TGATACACAT GCCAGACTGA 
     2161 GAGAATTCTG GATGCGCTAC TACTCTGCTC ATTACATGAC CTTAGTTGTT CAGTCCAAAG 
     2221 AAACCCTGGA CACTTTGGAA AAGTGGGTGA CGGAGATCTT CTCTCAGATA CCAAACAATG 
     2281 GGCTACCTAA ACCAAACTTT AGCCATTTAA CGGATCCTTT TGACACACCA GCATTTAACA 
     2341 AACTTTATAG AGTTGTTCCA ATCAGAAAAA TCCATGCTCT GACCATCACA TGGGCACTTC 
     2401 CTCCTCAGCA GCAGCACTAC AGGGTGAAGC CACTTCATTA TATTTCCTGG CTTGTTGGAC 
     2461 ATGAAGGCAA AGGCAGCATT CTTTCTTATC TTAGAAAAAA GTGCTGGGCT CTTGCATTGT 
     2521 TTGGTGGAAA TGGTGAGACA GGTTTTGAGC AAAATTCTAC TTACTCAGTG TTCAGCATTT 
     2581 CTATTACGCT GACTGATGAG GGCTATGAGC ATTTTTATGA GGTTGCCCAC ACTGTCTTTC 
     2641 AGTATTTAAA AATGCTGCAG AAGCTAGGTC CAGAGAAAAG AGTTTTTGAA GAGATTCAGA 
     2701 AAATTGAGGA TAATGAATTT CATTATCAAG AACAGACAGA TCCAGTCGAG TATGTGGAGA 
     2761 ACATGTGTGA GAACATGCAG CTGTACCCAC GTCAGGACTT TCTCACCGGA GATCAGCTTC 
     2821 TGTTTGAGTA CAAGCCAGAA GTCATTGCTG AGGCCCTTAA TCAGCTAGTT CCTCAGAAGG 
     2881 CAAATCTCGT TCTTCTCTCT GGTGCTAATG AGGGAAGATG TGACCTCAAG GAGAAGTGGT 
     2941 TTGGGACTCA GTATAGCATA GAAGATATTG AAAACTCTTG GACTGAACTA TGGAAGAGTA 
     3001 ATTTTGACTT AAATCCAGAC CTTCATCTTC CAGCTGAAAA TAAGTACATA GCCACGGACT 
     3061 TTACGTTGAA GGCTTTTGAT TGCCCTGAAA CTGAATACCC TGCTAAAATT GTCAATACCG 
     3121 CACAAGGCTG CTTGTGGTAT AAGAAAGACA ACAAATTCAA AATCCCCAAA GCATATATAC 
     3181 GATTTCATCT GATCTCACCT TTGATACAGA AATCTGCAGC AAATGTTGTC CTCTTTGACA 
     3241 TCTTCGTGAA TATTCTTACG CACAACCTTG CCGAACCCGC CTATGAAGCT GATGTGGCAC 
     3301 AGCTGGAGTA TAAACTGGTA GCTGGAGAAC ATGGGTTAAT TATTCGAGTG AAAGGATTCA 
     3361 ACCACAAACT ACCTCTGCTC TTTCAGCTCA TTATTGACTA CCTGACTGAG TTCAGTTCCA 
     3421 CACCCGCTGT CTTTACCATG ATAACTGAGC AACTGAAGAA GACCTACTTC AACATCCTCA 
     3481 TCAAGCCCGA GACTCTGGCC AAAGATGTGC GGCTTTTAAT TCTGGAATAT TCCCGCTGGT 
     3541 CTATGATTGA CAAGTACCAA GCTTTGATGG ATGGCCTTTC CCTTGATTCT CTGCTAAACT 
     3601 TTGTCAAGGA TTTTAAATCC CAACTTTTTG TTGAGGGCCT GGTCCAAGGG AATGTCACCA 
     3661 GCACGGAATC GATGGATTTC CTAAAATATG TTGTTGACAA GCTGAACTTC GCGCCCCTGG 
     3721 AGCGGGAGAT GCCTGTGCAG TTCCAGGTGG TGGAGCTGCC GAGCGGCCAC CACCTGTGCA 
     3781 AAGTCAGAGC TCTGAATAAA GGGGATGCCA ACTCTGAAGT CACTGTGTAC TACCAGTCAG 
     3841 GTACGAGGAG TCTGAGAGAA TATACTCTTA TGGAGCTGCT TGTGATGCAC ATGGAAGAGC 
     3901 CCTGCTTTGA CTTCCTTCGC ACCAAGCAGA CCCTTGGGTA CCATGTCTAC CCAACCTGTA 
     3961 GGAACACATC TGGAATTCTA GGATTTTCTG TAACTGTTGG GACTCAGGCA ACCAAATACA 
     4021 ACTCCGAAAC TGTTGACAAG AAGATAGAGG AATTTCTTTC CAGCTTTGAG GAGAAGATTG 
     4081 AGAACCTCAC AGAAGATGCA TTCAATACTC AGGTCACAGC ACTGATCAAG TTGAAAGAGT 
     4141 GTGAGGACAC CCACCTTGGG GAGGAGGTGG ACAGGAACTG GAATGAAGTG GTGACACAGC 
     4201 AGTATCTCTT TGACCGCCTT GCCCATGAGA TTGAAGCCCT GAAGTCTTTC TCAAAGTCAG 
     4261 ACCTGGTCAG CTGGTTCAAG GCTCACAGAG GACCTGGAAG TAAAATGCTC AGCGTTCATG 
     4321 TTGTTGGATA TGGGAAGTAT GAACTGGAAG AGGATGGTGC CCCTTTTGGT GAGGATTCAA 
     4381 ATTCTCGTGA AGGGATGCAG CTAACCTACC TGCCACCCTC TCCTGTCCTG GCAGAATCCA 
     4441 CCACCCCCAT TACTGACATC AGGGCCTTCA CAGCAACACT CAGCCTCTTC CCCTACCATA 
     4501 AAATAGTCAA AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4561 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4621 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4681 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4741 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4801 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4861 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4921 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4981 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5041 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5101 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5161 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5221 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5281 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5341 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5401 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5461 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5521 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5581 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5641 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5701 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5761 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5821 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5881 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5941 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6001 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6061 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6121 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6181 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6241 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6301 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6361 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6421 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6481 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6541 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6601 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6661 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6721 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6781 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6841 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6901 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6961 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7021 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7081 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7141 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7201 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7261 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7321 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7381 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7441 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7501 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7561 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7741 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7801 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7861 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7921 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7981 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8041 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8101 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8161 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8221 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8281 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8341 TAACAAAAAT TTAACGCGAA TTTT
//