LOCUS       MR211704                8313 bp    DNA     circular  SYN 
DEFINITION  Nos2 (Myc-DDK-tagged) - Mouse nitric oxide synthase 2, inducible (Nos2)
ACCESSION   NM_010927
VERSION     NM_010927.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4470..4561 
                     /label="Myc-DDK"  
     misc_feature    4591..5176 
                     /label="PolyA signal"  
     misc_feature    4648..4667 
                     /label="Restriction Enzyme"  
     misc_feature    5452..6124 
                     /label="ColE1"  
     misc_feature    6554..7348 
                     /label="Kan/Neo"  
     misc_feature    7383..7685 
                     /label="SV40 ori"  
     misc_feature    7771..8248 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4452..4458 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4452 
                     /label="Nos2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTGCCCC TGGAAGTTTC TCTTCAAAGT CAAATCCTAC CAAAGTGACC 
     1081 TGAAAGAGGA AAAGGACATT AACAACAACG TGAAGAAAAC CCCTTGTGCT GTTCTCAGCC 
     1141 CAACAATACA AGATGACCCT AAGAGTCACC AAAATGGCTC CCCGCAGCTC CTCACTGGGA 
     1201 CAGCACAGAA TGTTCCAGAA TCCCTGGACA AGCTGCATGT GACATCGACC CGTCCACAGT 
     1261 ATGTGAGGAT CAAAAACTGG GGCAGTGGAG AGATTTTGCA TGACACTCTT CACCACAAGG 
     1321 CCACATCGGA TTTCACTTGC AAGTCCAAGT CTTGCTTGGG GTCCATCATG AACCCCAAGA 
     1381 GTTTGACCAG AGGACCCAGA GACAAGCCTA CCCCTCTGGA GGAGCTCCTG CCTCATGCCA 
     1441 TTGAGTTCAT CAACCAGTAT TATGGCTCCT TTAAAGAGGC AAAAATAGAG GAACATCTGG 
     1501 CCAGGCTGGA AGCTGTAACA AAGGAAATAG AAACAACAGG AACCTACCAG CTCACTCTGG 
     1561 ATGAGCTCAT CTTTGCCACC AAGATGGCCT GGAGGAATGC CCCTCGCTGC ATCGGCAGGA 
     1621 TCCAGTGGTC CAACCTGCAG GTCTTTGACG CTCGGAACTG TAGCACAGCA CAGGAAATGT 
     1681 TTCAGCACAT CTGCAGACAC ATACTTTATG CCACCAACAA TGGCAACATC AGGTCGGCCA 
     1741 TCACTGTGTT CCCCCAGCGG AGTGACGGCA AACATGACTT CAGGCTCTGG AATTCACAGC 
     1801 TCATCCGGTA CGCTGGCTAC CAGATGCCCG ATGGCACCAT CAGAGGGGAT GCTGCCACCT 
     1861 TGGAGTTCAC CCAGTTGTGC ATCGACCTAG GCTGGAAGCC CCGCTATGGC CGCTTTGATG 
     1921 TGCTGCCTCT GGTCTTGCAA GCTGATGGTC AAGATCCAGA GGTCTTTGAA ATCCCTCCTG 
     1981 ATCTTGTGTT GGAGGTGACC ATGGAGCATC CCAAGTACGA GTGGTTCCAG GAGCTCGGGT 
     2041 TGAAGTGGTA TGCACTGCCT GCCGTGGCCA ACATGCTACT GGAGGTGGGT GGCCTCGAAT 
     2101 TCCCAGCCTG CCCCTTCAAT GGTTGGTACA TGGGCACCGA GATTGGAGTT CGAGACTTCT 
     2161 GTGACACACA GCGCTACAAC ATCCTGGAGG AAGTGGGCCG AAGGATGGGC CTGGAGACCC 
     2221 ACACACTGGC CTCCCTCTGG AAAGACCGGG CTGTCACGGA GATCAATGTG GCTGTGCTCC 
     2281 ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CACAGCCTCA GAGTCCTTCA 
     2341 TGAAGCACAT GCAGAATGAG TACCGGGCCC GTGGAGGCTG CCCGGCAGAC TGGATTTGGC 
     2401 TGGTCCCTCC AGTGTCTGGG AGCATCACCC CTGTGTTCCA CCAGGAGATG TTGAACTATG 
     2461 TCCTATCTCC ATTCTACTAC TACCAGATCG AGCCCTGGAA GACCCACATC TGGCAGAATG 
     2521 AGAAGCTGAG GCCCAGGAGG AGAGAGATCC GATTTAGAGT CTTGGTGAAA GTGGTGTTCT 
     2581 TTGCTTCCAT GCTAATGCGA AAGGTCATGG CTTCACGGGT CAGAGCCACA GTCCTCTTTG 
     2641 CTACTGAGAC AGGGAAGTCT GAAGCACTAG CCAGGGACCT GGCCACCTTG TTCAGCTACG 
     2701 CCTTCAACAC CAAGGTTGTC TGCATGGACC AGTATAAGGC AAGCACCTTG GAAGAGGAGC 
     2761 AACTACTGCT GGTGGTGACA AGCACATTTG GGAATGGAGA CTGTCCCAGC AATGGGCAGA 
     2821 CTCTGAAGAA ATCTCTGTTC ATGCTTAGAG AACTCAACCA CACCTTCAGG TATGCTGTGT 
     2881 TTGGCCTTGG CTCCAGCATG TACCCTCAGT TCTGCGCCTT TGCTCATGAC ATCGACCAGA 
     2941 AGCTGTCCCA CCTGGGAGCC TCTCAGCTTG CCCCAACAGG AGAAGGGGAC GAACTCAGTG 
     3001 GGCAGGAGGA TGCCTTCCGC AGCTGGGCTG TACAAACCTT CCGGGCAGCC TGTGAGACCT 
     3061 TTGATGTCCG AAGCAAACAT CACATTCAGA TCCCGAAACG CTTCACTTCC AATGCAACAT 
     3121 GGGAGCCACA GCAATATAGG CTCATCCAGA GCCCGGAGCC TTTAGACCTC AACAGAGCCC 
     3181 TCAGCAGCAT CCATGCAAAG AACGTGTTTA CCATGAGGCT GAAATCCCAG CAGAATCTGC 
     3241 AGAGTGAAAA GTCCAGCCGC ACCACCCTCC TCGTTCAGCT CACCTTCGAG GGCAGCCGAG 
     3301 GGCCCAGCTA CCTGCCTGGG GAACACCTGG GGATCTTCCC AGGCAACCAG ACCGCCCTGG 
     3361 TGCAGGGAAT CTTGGAGCGA GTTGTGGATT GTCCTACACC ACACCAAACT GTGTGCCTGG 
     3421 AGGTTCTGGA TGAGAGCGGC AGCTACTGGG TCAAAGACAA GAGGCTGCCC CCCTGCTCAC 
     3481 TCAGCCAAGC CCTCACCTAC TTCCTGGACA TTACGACCCC TCCCACCCAG CTGCAGCTCC 
     3541 ACAAGCTGGC TCGCTTTGCC ACGGACGAGA CGGATAGGCA GAGATTGGAG GCCTTGTGTC 
     3601 AGCCCTCAGA GTACAATGAC TGGAAGTTCA GCAACAACCC CACGTTCCTG GAGGTGCTTG 
     3661 AAGAGTTCCC TTCCTTGCAT GTGCCCGCTG CCTTCCTGCT GTCGCAGCTC CCTATCTTGA 
     3721 AGCCCCGCTA CTACTCCATC AGCTCCTCCC AGGACCACAC CCCCTCGGAG GTTCACCTCA 
     3781 CTGTGGCCGT GGTCACCTAC CGCACCCGAG ATGGTCAGGG TCCCCTGCAC CATGGTGTCT 
     3841 GCAGCACTTG GATCAGGAAC CTGAAGCCCC AGGACCCAGT GCCCTGCTTT GTGCGAAGTG 
     3901 TCAGTGGCTT CCAGCTCCCT GAGGACCCCT CCCAGCCTTG CATCCTCATT GGGCCTGGTA 
     3961 CGGGCATTGC TCCCTTCCGA AGTTTCTGGC AGCAGCGGCT CCATGACTCC CAGCACAAAG 
     4021 GGCTCAAAGG AGGCCGCATG AGCTTGGTGT TTGGGTGCCG GCACCCGGAG GAGGACCACC 
     4081 TCTATCAGGA AGAAATGCAG GAGATGGTCC GCAAGAGAGT GCTGTTCCAG GTGCACACAG 
     4141 GCTACTCCCG GCTGCCCGGC AAACCCAAGG TCTACGTTCA GGACATCCTG CAAAAGCAGC 
     4201 TGGCCAATGA GGTACTCAGC GTGCTCCACG GGGAGCAGGG CCACCTCTAC ATTTGCGGAG 
     4261 ATGTGCGCAT GGCTCGGGAT GTGGCTACCA CATTGAAGAA GCTGGTGGCC ACCAAGCTGA 
     4321 ACTTGAGCGA GGAGCAGGTG GAAGACTATT TCTTCCAGCT CAAGAGCCAG AAACGTTATC 
     4381 ATGAAGATAT CTTCGGTGCA GTCTTTTCCT ATGGGGCAAA AAAGGGCAGC GCCTTGGAGG 
     4441 AGCCCAAAGC CACGAGGCTC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4501 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4561 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4621 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4681 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4741 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4801 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4861 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4921 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4981 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5041 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5101 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5161 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5221 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5281 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5341 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5401 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5461 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5521 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5581 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5641 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5701 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5761 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5821 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5881 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5941 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6001 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6061 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6121 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6181 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6241 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6301 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6361 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6421 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6481 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6541 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6601 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6661 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6721 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6781 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6841 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6901 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6961 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7021 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7081 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7141 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7201 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7261 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7321 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7381 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7441 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7501 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7561 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7621 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7681 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7741 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7801 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7861 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7921 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7981 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8041 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8101 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8161 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8221 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8281 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//