LOCUS       MR211688                8283 bp    DNA     circular  SYN 
DEFINITION  Srebf1 (Myc-DDK-tagged) - Mouse sterol regulatory element binding transcription factor 1 (Srebf1)
ACCESSION   NM_011480
VERSION     NM_011480.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4440..4531 
                     /label="Myc-DDK"  
     misc_feature    4561..5146 
                     /label="PolyA signal"  
     misc_feature    4618..4637 
                     /label="Restriction Enzyme"  
     misc_feature    5422..6094 
                     /label="ColE1"  
     misc_feature    6524..7318 
                     /label="Kan/Neo"  
     misc_feature    7353..7655 
                     /label="SV40 ori"  
     misc_feature    7741..8218 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4422..4428 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4422 
                     /label="Srebf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGAGCTG GCCTTCGGTG AGGCGGCTCT GGAACAGACA CTGGCCGAGA 
     1081 TGTGCGAACT GGACACAGCG GTTTTGAACG ACATCGAAGA CATGCTCCAG CTCATCAACA 
     1141 ACCAAGACAG TGACTTCCCG GGCCTGTTTG ACGCCCCCTA TGCTGGGGGT GAGACAGGGG 
     1201 ACACAGGCCC CAGCAGCCCA GGTGCCAACT CTCCTGAGAG CTTCTCTTCT GCTTCTCTGG 
     1261 CCTCCTCTCT GGAAGCCTTC CTGGGAGGAC CCAAGGTGAC ACCTGCACCC TTGTCCCCTC 
     1321 CACCATCGGC ACCCGCTGCT TTAAAGATGT ACCCGTCCGT GTCCCCCTTT TCCCCTGGGC 
     1381 CTGGGATCAA AGAGGAGCCA GTGCCACTCA CCATCCTACA GCCTGCAGCG CCACAGCCGT 
     1441 CACCGGGGAC CCTCCTGCCT CCGAGCTTCC CCGCACCACC CGTACAGCTC AGCCCTGCGC 
     1501 CCGTGCTGGG TTACTCGAGC CTGCCTTCAG GCTTCTCAGG GACCCTTCCA GGAAACACTC 
     1561 AGCAGCCACC ATCTAGCCTG CCGCTGGCCC CTGCACCAGG AGTCTTGCCC ACCCCTGCCC 
     1621 TGCACACCCA GGTCCAAAGC TTGGCCTCCC AGCAGCCGCT GCCAGCCTCA GCAGCCCCTA 
     1681 GAACAAACAC TGTGACCTCA CAGGTCCAGC AGGTCCCAGT TGTACTGCAG CCACACTTCA 
     1741 TCAAGGCAGA CTCACTGCTG CTGACAGCTG TGAAGACAGA TGCAGGAGCC ACCGTGAAGA 
     1801 CTGCAGGCAT CAGCACCCTG GCTCCTGGCA CAGCCGTGCA GGCAGGTCCC CTGCAGACCC 
     1861 TGGTGAGTGG AGGGACCATC TTGGCCACAG TACCTTTGGT TGTGGACACA GACAAACTGC 
     1921 CCATCCACCG ACTCGCAGCT GGCAGCAAGG CCCTAGGCTC AGCTCAGAGC CGTGGTGAGA 
     1981 AGCGCACAGC CCACAATGCC ATTGAGAAGC GCTACCGGTC TTCTATCAAT GACAAGATTG 
     2041 TGGAGCTCAA AGACCTGGTG GTGGGCACTG AAGCAAAGCT GAATAAATCT GCTGTCTTGC 
     2101 GCAAGGCCAT CGACTACATC CGCTTCTTGC AGCACAGCAA CCAGAAGCTC AAGCAGGAGA 
     2161 ACCTGACCCT ACGAAGTGCA CACAAAAGCA AATCACTGAA GGACCTGGTG TCAGCTTGTG 
     2221 GCAGTGGAGG AGGCACAGAT GTGTCTATGG AGGGCATGAA ACCCGAAGTG GTGGAGACGC 
     2281 TTACCCCTCC ACCCTCAGAC GCCGGCTCAC CCTCCCAGAG TAGCCCCTTG TCTTTTGGCA 
     2341 GCAGAGCTAG CAGCAGTGGT GGTAGTGACT CTGAGCCCGA CAGTCCAGCC TTTGAGGATA 
     2401 GCCAGGTCAA AGCCCAGCGG CTGCCTTCAC ACAGCCGAGG CATGCTGGAC CGCTCCCGCC 
     2461 TGGCCCTGTG TGTACTGGCC TTTCTGTGTC TGACCTGCAA TCCTTTGGCC TCGCTTTTCG 
     2521 GCTGGGGCAT TCTCACTCCC TCTGATGCTA CGGGTACACA CCGTAGTTCT GGGCGCAGCA 
     2581 TGCTGGAGGC AGAGAGCAGA GATGGCTCTA ATTGGACCCA GTGGTTGCTG CCACCCCTAG 
     2641 TCTGGCTGGC CAATGGACTA CTAGTGTTGG CCTGCTTGGC TCTTCTCTTT GTCTATGGGG 
     2701 AACCTGTGAC TAGGCCACAC TCTGGCCCGG CTGTACACTT CTGGAGACAT CGCAAACAAG 
     2761 CTGACCTGGA TTTGGCCCGG GGAGATTTCC CCCAGGCTGC TCAACAGCTG TGGCTGGCCC 
     2821 TGCAAGCGCT GGGCCGGCCC CTGCCCACCT CAAACCTGGA TCTGGCCTGC AGTCTGCTTT 
     2881 GGAACCTCAT CCGCCACCTG CTCCAGCGTC TCTGGGTGGG CCGCTGGCTG GCAGGCCAGG 
     2941 CCGGGGGCCT GCTGAGGGAC CGTGGGCTGA GGAAGGATGC CCGTGCCAGT GCCCGGGATG 
     3001 CGGCTGTTGT CTACCATAAG CTGCACCAGC TGCATGCCAT GGGCAAGTAC ACAGGAGGAC 
     3061 ATCTTGCTGC TTCTAACCTG GCACTAAGTG CCCTCAACCT GGCTGAGTGC GCAGGAGATG 
     3121 CTATCTCCAT GGCAACACTG GCAGAGATCT ATGTGGCAGC GGCCCTGAGG GTCAAAACCA 
     3181 GCCTCCCAAG AGCCCTGCAC TTCTTGACAC GTTTCTTCCT GAGCAGCGCC CGCCAGGCCT 
     3241 GCCTAGCACA GAGCGGCTCG GTGCCTCTTG CCATGCAGTG GCTCTGCCAC CCTGTAGGTC 
     3301 ACCGTTTCTT TGTGGACGGG GACTGGGCCG TGCACGGTGC CCCCCCGGAG AGCCTGTACA 
     3361 GCGTGGCTGG GAACCCAGTG GATCCGCTGG CCCAGGTGAC CCGGCTATTC CGTGAACATC 
     3421 TCCTAGAGCG AGCGTTGAAC TGTATTGCTC AGCCCAGCCC AGGGGCAGCT GACGGAGACA 
     3481 GGGAGTTCTC AGATGCCCTT GGATATCTGC AGTTGCTAAA TAGCTGTTCT GATGCTGCCG 
     3541 GGGCTCCTGC TTGCAGTTTC TCTGTCAGCT CCAGCATGGC TGCCACCACT GGCCCAGACC 
     3601 CAGTGGCCAA GTGGTGGGCC TCACTGACAG CTGTGGTGAT CCACTGGCTG AGGCGGGATG 
     3661 AAGAGGCAGC TGAGCGCTTG TACCCACTGG TAGAGCATAT CCCCCAGGTG CTGCAGGACA 
     3721 CTGAGAGACC CCTGCCCAGG GCAGCTCTGT ACTCCTTCAA GGCTGCCCGG GCTCTGCTGG 
     3781 ACCACAGAAA GGTGGAATCT AGCCCAGCCA GCCTGGCCAT CTGTGAGAAG GCCAGTGGGT 
     3841 ACCTGCGGGA CAGCTTAGCC TCTACACCAA CTGGCAGTTC CATTGACAAG GCCATGCAGC 
     3901 TGCTCCTGTG TGATCTACTT CTTGTGGCCC GTACCAGTCT GTGGCAGCGG CAGCAGTCAC 
     3961 CAGCTTCAGT CCAGGTAGCT CACGGTACCA GCAATGGACC CCAGGCCTCT GCTCTGGAGC 
     4021 TGCGTGGTTT CCAACATGAC CTGAGCAGCC TGCGGCGGTT GGCACAGAGC TTCCGGCCTG 
     4081 CTATGAGGAG GGTATTCCTA CATGAGGCCA CAGCTCGGCT GATGGCAGGA GCAAGTCCTG 
     4141 CCCGGACACA CCAGCTCCTG GATCGCAGTC TGAGGAGGAG GGCAGGTTCC AGTGGCAAAG 
     4201 GAGGCACTAC AGCTGAGCTG GAGCCACGGC CCACATGGCG GGAGCACACC GAGGCCCTGC 
     4261 TGTTGGCATC CTGCTATCTG CCCCCTGCCT TCCTGTCGGC TCCTGGGCAG CGAATGAGCA 
     4321 TGCTGGCCGA GGCGGCACGC ACCGTAGAGA AGCTTGGCGA TCACCGGCTA CTGCTGGACT 
     4381 GCCAGCAGAT GCTCCTGCGC CTGGGCGGCG GAACCACCGT CACTTCCAGC ACGCGTACGC 
     4441 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4501 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4561 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4621 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4681 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4741 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4801 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4861 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4921 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4981 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5041 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5101 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5161 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5221 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5281 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5341 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5401 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5461 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5521 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5581 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5641 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5701 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5761 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5821 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5881 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5941 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6001 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6061 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6121 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6181 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6241 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6301 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6361 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6421 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6481 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6541 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6601 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6661 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6721 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6781 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6841 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6901 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6961 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7021 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7081 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7141 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7201 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7261 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7321 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7381 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7441 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7501 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7561 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7621 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7681 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7741 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7801 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7861 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7921 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7981 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8041 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8101 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8161 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8221 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8281 TTT
//