LOCUS       MR211607                8136 bp    DNA     circular  SYN 
DEFINITION  Phkb (Myc-DDK-tagged) - Mouse phosphorylase kinase beta (Phkb)
ACCESSION   NM_199446
VERSION     NM_199446.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4293..4384 
                     /label="Myc-DDK"  
     misc_feature    4414..4999 
                     /label="PolyA signal"  
     misc_feature    4471..4490 
                     /label="Restriction Enzyme"  
     misc_feature    5275..5947 
                     /label="ColE1"  
     misc_feature    6377..7171 
                     /label="Kan/Neo"  
     misc_feature    7206..7508 
                     /label="SV40 ori"  
     misc_feature    7594..8071 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4275..4281 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4275 
                     /label="Phkb"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAACTCA CCTGATGCAG CCTTCTCTTC ACCTGCTTTG TTAAGGTCTG 
     1081 GCTCAGTTTA TGAACCTCTT AAAAGCATTA ATCTTCCAAG ACCTGATAAT GAAACTCTAT 
     1141 GGGATAAGTT GGATCATTAT TACAGAATTG TCAAGTCAAC GATGCTGATG TATCAAAGTC 
     1201 CAACTACCGG TCTCTTTCCT ACTAAAACAT GTGGTGGAGA AGAGAAGTCC AAAGTGCACG 
     1261 AGAGTCTGTA CTGTGCTGCG GGTGCCTGGG CCTTGGCGCT AGCATACAGG CGCATTGATG 
     1321 ATGACAAGGG ACGGACCCAT GAGCTGGAGC ACTCGGCCAT AAAGTGTATG AGAGGAATTC 
     1381 TCTACTGCTA CATGCGGCAG GCTGATAAGG TCCAACAGTT TAAACAAGAT CCCCGGCCCA 
     1441 CAACATGTCT TCACTCTGTT TTCAGTGTGC ACACTGGGGA TGAGTTGCTC AGCTATGAAG 
     1501 AATATGGCCA TCTTCAGATA AATGCAGTCT CACTCTTTCT CCTTTACCTG GTAGAAATGA 
     1561 TTTCCTCAGG CCTCCAGATA ATCTATAACA CGGATGAGGT TTCTTTTATT CAAAACCTTG 
     1621 TATTTTGTGT AGAGAGAGTT TACCGTGTTC CTGACTTTGG TGTCTGGGAA AGAGGAAGCA 
     1681 AATATAACAA TGGTAGTACC GAACTGCACT CAAGCTCTGT TGGTTTAGCA AAAGCAGCCT 
     1741 TAGAAGCAAT TAATGGATTT AACCTCTTTG GAAACCAGGG GTGTTCCTGG TCAGTTATAT 
     1801 TTGTGGATCT CGATGCTCAC AACCGCAACA GGCAGACATT GTGCTCACTG TTACCCAGAG 
     1861 AATCACGCTC TCATAACACA GATGCTGCCC TGCTCCCGTG CATCAGTTAC CCTGCGTTTG 
     1921 CCTTGGATGA TGAAGCACTG TTCAGCCAGA CACTTGATAA AGTGATAAGA AAATTGAAAG 
     1981 GAAAATATGG ATTTAAGCGT TTCTTGCGAG ATGGGTACAG AACGCCATTG GAAGATCCCA 
     2041 ACAGACGCTA TTACAAACCA GCAGAGATTA AGCTGTTTGA TGGCATTGAA TGTGAATTTC 
     2101 CCATATTCTT CCTTTACATG ATGATCGATG GTGTTTTTAG AGGCAATCTT GAACAAGTCA 
     2161 AGGAATATCA GGACCTTTTG ACACCTTTGC TTCATCAGAC CACAGAAGGA TATCCTGTTG 
     2221 TGCCAAAGTA CTATTATGTG CCAGCTGACT TTGTAGAATG TGAAAAAAGA AACCCTGGTA 
     2281 GTCAGAAGCG GTTTCCAAGT AACTGTGGCC GTGATGGGAA GTTGTTTCTT TGGGGACAAG 
     2341 CTCTTTATAT CATTGCAAAA CTCCTAGCTG ATGAGTTAAT CAGCCCTAAA GACATTGACC 
     2401 CTGTCCAGCG CTTTGTCCCA CTTCAGAATC AACGTAACGT GAGCATGAGA TACTCCAATC 
     2461 AGGGCCCACT GGAAAATGAC TTGGTGGTTC ATGTAGCACT TGTAGCAGAA AGCCAACGCC 
     2521 TTCAAGTTTT TCTCAATACA TATGGTATTC AAACCCAAAC TCCTCAGCAG GTGGAACCCA 
     2581 TTCAGATCTG GCCTCAGCAG GAGCTTGTAA AAGCTTATTT CCACCTGGGA ATCAATGAAA 
     2641 AGTTGGGTCT GTCTGGAAGG CCAGATAGGC CCATTGGCTG CCTTGGTACA TCAAAGATTT 
     2701 ATCGCATTCT AGGAAAGACT GTGGTTTGTT ACCCTATCAT CTTTGACCTG AGTGATTTCT 
     2761 ACATGTCTCA GGACGTTCTG CTGCTCATAG ATGACATAAA GAACGCACTA CAGTTCATTA 
     2821 AGCAATATTG GAAAATGCAT GGACGCCCAC TTTTTCTTGT TCTCATCCGA GAAGACAACA 
     2881 TAAGAGGTAG CAGATTCAAC CCAATATTAG ACATGCTGGC AGCCTTTAAA AAGGGAATAA 
     2941 TTGGAGGAGT CAAAGTTCAT GTTGATCGTT TACAGACATT GATATCCGGA GCTGTAGTAG 
     3001 AGCAGCTTGA CTTTCTACGA ATTAGTGACA CAGAAAAGCT TCCAGAATTT AAGAGCTTTG 
     3061 AAGAACTAGA ATTTCCTAAG CATTCAAAAG TCAAACGGCA GAGCAGTACC GCAGATGCTC 
     3121 CTGAGGCGCA ACACGAGCCA GGCATCACCA TTACCGAGTG GAAAAACAAA TCCACCCACG 
     3181 AAATCCTTCA GAAGCTGAAT GACTGCGGTT GTCTGGCTGG TCAAACCATC CTGCTGGGTA 
     3241 TACTGCTCAA AAGAGAGGGC CCTAACTTTA TCACAATGGA AGGAACTGTG TCTGATCACA 
     3301 TTGAGAGAGT GTATAGAAGA GCTGGCAGCA AAAAGCTTTG GTCGGTTGTA CGCCGTGCAG 
     3361 CAAGTCTTTT AAATAAAGTA GTGGACAGCC TGGCCCCATC CATTACTAAT GTTTTAGTGC 
     3421 AGGGCAAACA GGTAACTCTA GGTGCCTTTG GACATGAAGA GGAGGTCATC TCTAACCCAC 
     3481 TGTCTCCAAG AGTGATTAAG AACATAATCT ATTATAAGTG CAATACCCAC GATGAGAGGG 
     3541 AGGCAGTCAT TCAGCAAGAA CTGGTCATCC ACATCGGCTG GATCATCTCC AACAGCCCTG 
     3601 AGTTGTTCAG CGGCATGCTG AAAATACGCA TTGGGTGGAT CATCCACGCC ATGGAGTATG 
     3661 AACTGCAGGT CCGGGGCGGG GATAAGCCAG CTGTGGACTT GTACCAGCTC TCGCCCAGTG 
     3721 AAGTGAAGCA GCTGCTGCTA GACATTCTCC AGCCTCAACA GAGTGGAAGA TGTTGGTTGA 
     3781 ATAGACGTCA GATTGATGGG TCATTGAATA GAACACCCCC GGAATTCTAT GACCGAGTGT 
     3841 GGCAGATCCT GGAACGCACA CCTAATGGTA TCGTTGTAGC TGGAAAGCAT TTGCCACAGC 
     3901 AACCAACTTT GTCGGATATG ACCATGTATG AGATGAATTT TTCTCTCCTT GTTGAAGATA 
     3961 TGCTGGGAAA CATTGACCAG CCAAAGTATA GACAGATCAT AGTTGAGTTA CTAATGGTCG 
     4021 TATCCATTGT GCTGGAAAGA AACCCTGAAC TGGAGTTTCA AGACAAAGTG GATCTGGACA 
     4081 GACTGGTTAA AGAAGCGTTT CATGAGTTTC AAAAAGATGA GAGTCGGCTA AAAGAAATCG 
     4141 AAAAACAAGA TGATATGACT TCCTTTTACA ACACGCCCCC ACTGGGAAAA AGAGGAACCT 
     4201 GCAGCTACTT GACGAAGGTT GTGATGAACT CACTGCTCGA GGGAGAAGTC AAGCCCAGCA 
     4261 ATGAGGACTC CTGCCTGGTT AGCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4321 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4381 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4441 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4501 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4561 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4621 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4681 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4741 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4801 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4861 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4921 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4981 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5041 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5101 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5161 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5221 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5281 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5341 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5401 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5461 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5521 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5581 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5641 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5701 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5761 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5821 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5881 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5941 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6001 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6061 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6121 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6181 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6241 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6301 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6361 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6421 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6481 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6541 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6601 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6661 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6721 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6781 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6841 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6901 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6961 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7021 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7081 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7141 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7201 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7261 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7321 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7381 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7441 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7501 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7561 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7621 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7681 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7741 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7801 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7861 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7921 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7981 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8041 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8101 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//