LOCUS       MR211548                8040 bp    DNA     circular  SYN 
DEFINITION  Itga3 (Myc-DDK-tagged) - Mouse integrin alpha 3 (Itga3)
ACCESSION   NM_013565
VERSION     NM_013565.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4197..4288 
                     /label="Myc-DDK"  
     misc_feature    4318..4903 
                     /label="PolyA signal"  
     misc_feature    4375..4394 
                     /label="Restriction Enzyme"  
     misc_feature    5179..5851 
                     /label="ColE1"  
     misc_feature    6281..7075 
                     /label="Kan/Neo"  
     misc_feature    7110..7412 
                     /label="SV40 ori"  
     misc_feature    7498..7975 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4179..4185 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4179 
                     /label="Itga3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCCCCGGC CCCTGCCGCG TGCCCCGCGC CCCTGGCTGG CTGCTCCGCG 
     1081 CTCTGGCCTT GATGGTGGCG GCTTGCGGCC GGGTCGCCTT CGCCTTCAAC CTGGACACCC 
     1141 GATTCCTGGT GGTGAAGGAG GCGGTGAACC CCGGTAGCCT CTTCGGCTAC TCGGTCGCCC 
     1201 TCCATCGGCA GACTGAGCGA CAACAGCGCT ACCTCCTTCT GGCCGGGGCT CCCCGGGACC 
     1261 TCGCTGTGGG TGATGACTAT ACCAACCGGA CTGGTGCTGT GTACCTGTGT CCCCTCACGG 
     1321 CCCACAAGGA CGACTGTGAA CGGATGGACA TTTCAGAGAA AAGTGACCCT GACCATCACA 
     1381 TTATTGAGGA TATGTGGCTT GGAGTGACTG TGGCCAGCCA GGGCCCTGCA GGTAGAGTCC 
     1441 TGGTCTGTGC CCATCGGTAC ACCAAGGTGC TGTGGTCTGG GCTAGAAGAC CAGCGGCGCA 
     1501 TGGTGGGCAA GTGCTATGTG CGTGGCAATG ACCTACAGCT CGACCCCGGC GATGACTGGC 
     1561 AGACATACCA CAACGAGATG TGTAACAGCA ACACTGACTA CTTGCAGACC GGCATGTGCC 
     1621 AGCTGGGCAC CAGCGGCGGC TTCACCCAGA ACACCGTATA CTTCGGTGCC CCTGGTGCCT 
     1681 ACAACTGGAA AGGAAACAGC TACATGATTC AGCGGAAGGA CTGGGATTTA TCTGAATATA 
     1741 GCTACAGGGG CTCAGAGGAG CAAGGAAACC TTTATATTGG GTACACGGTG CAGGTAGGCA 
     1801 ACGCCATCCT ACATCCCACG GACATCATCA CTGTTGTGAC GGGTGCCCCA CGGCACCAAC 
     1861 ATATGGGCGC TGTCTTCTTG CTGAAGCAAG AGTCAGGTGG AGACCTGCAG AGGAAGCAGG 
     1921 TGCTGAAGGG CACGCAGGTG GGCGCTTATT TTGGCAGTGC CATTGCCCTG GCAGACCTGA 
     1981 ACAATGATGG GTGGCAGGAC CTCCTGGTGG GTGCTCCCTA CTACTTCGAA CGGAAAGAGG 
     2041 AGGTAGGGGG TGCCGTCTAT GTCTTCATGA ACCAGGCGGG CGCATCCTTC CCTGATCAAC 
     2101 CTTCCCTCCT GCTTCACGGC CCCAGCCGCT CTGCCTTTGG CATCTCTATA GCCAGCATCG 
     2161 GTGACATCAA CCAGGATGGA TTCCAGGACA TCGCTGTGGG AGCCCCATTT GAGGGCTTGG 
     2221 GCAAAGTCTA CATCTACCAC AGCAGCTCCG GGGGGCTCCT CAGGCAGCCC CAGCAGATAA 
     2281 TCCATGGAGA GAAACTCGGA CTGCCAGGCT TGGCCACCTT CGGCTACTCC CTGAGCGGGA 
     2341 AGATGGATGT GGATGAAAAC CTTTACCCAG ACCTGCTAGT AGGGAGCCTG TCGGACCACA 
     2401 TCGTGCTGCT GCGGGCCCGG CCTGTCATCA ATATCCTCCA TAGGACCTTG GTGGCCAGGC 
     2461 CAGCTGTGTT GGACCCTGCG CTTTGTACAG CTACCTCCTG TGTTCAGGTG GAGCTGTGTT 
     2521 TTGCCTACAA CCAGAGCGCT GGGAACCCCA ACTACAGGCG GAACATCACC CTGGCTTACA 
     2581 CACTGGAGGC TGACAGGGAC CGACGCCCAC CCAGGCTCCG ATTTGCCCGC AGCCAGTCAT 
     2641 CTGTCTTCCA CGGCTTCTTC TCCATGCCAG AGACACATTG CCAGACACTG GAGTTACTGC 
     2701 TGATGGACAA TGTTCGCGAT AAACTCCGTC CTATCGTCAT TGCCATGAAC TACTCCTTAC 
     2761 CTCTGCGCAT GCCTGATCGC CTCAAGCTCG GCTTGCGGTC TCTGGATGCC TACCCAGTCC 
     2821 TCAACCAGGC ACAGGCTATG GAGAATCACA CTGAGGTCCA CTTCCAGAAA GAGTGCGGGC 
     2881 CAGACAACAA GTGCGACAGC AACCTGCAGA TGCGAGCAGC CTTCCTGTCT GAGCAGCTGC 
     2941 AGCCTCTAAG CAGGCTCCAG TACAGCAGAG ACACTAAGAA ACTGTTTTTG AGCATCAATG 
     3001 TGACCAATTC ACCGAGCAGC CAACGGGCTG GGGAAGATGC GCATGAGGCA TTGCTCACCC 
     3061 TAGAGGTGCC ATCCGCCCTG CTACTGTCCT CCGTGCGCCC GTCTGGGACC TGCCAAGCTA 
     3121 ATAACGAGAC CATCCTGTGT GAGCTAGGAA ACCCCTTCAA ACGGAACCAG AGGATGGAGC 
     3181 TGCTCATTGC CTTTGAGGTC ATCGGGGTAA CGTTGCACAC GAGGGACCTC CCAGTACTTC 
     3241 TTCAGCTGTC CACGTCGAGT CACCAGGACA ACTTGCAGCC CGTACTCCTG ACTTTGCAGG 
     3301 TGGACTACAC CCTCCAGGCC TCGCTCAGCT TAATGAATCA TCGGTTGCAG AGCTTCTTTG 
     3361 GTGGCACAGT GATGGGTGAG GCTGCCATGA AAACTGCGGA GGATGTGGGA AGTCCCCTGA 
     3421 AATATGAATT CCAGGTGAGC CCAGTGGGAG ATGGGCTGGC AGCCTTGGGC ACACTGGTTC 
     3481 TAGGTCTGGA GTGGCCCTAT GAAGTTACCA ATGGCAAGTG GCTGCTGTAC CCCACGGAGA 
     3541 TCACCATCCA TAGCAATGGT TCCTGGCCCT GCCAGCCGTC TGGAAACCTT GTCAACCCTC 
     3601 TCAACCTTAC TCTCTCTGAC CCTGGAGTCA CACCACTGTC GCCACAGCGC CGCCGGCGGC 
     3661 AACTGGATCC AGGGGGAGAC CAGAGTTCCC CGCCTGTCAC ACTAGCTGCT GCCAAAAAAG 
     3721 CCAAGTCTGA GACTGTGTTG ACCTGCTCCA ATGGCCGTGC CCGCTGTGTG TGGCTGGAGT 
     3781 GCCCCCTTCC AGACACCTCC AACATTACCA ATGTGACCGT GAAAGCACGG GTGTGGAACA 
     3841 GCACCTTCAT TGAGGACTAC AAAGACTTTG ACAGAGTCAG GGTAGATGGC TGGGCTACCC 
     3901 TGTTCCTGAG AACCAGCATC CCTACCATCA ACATGGAGAA CAAGACCACA TGGTTCTCTG 
     3961 TGGACATTGA CTCAGAGCTG GTGGAGGAGC TGCCGGCTGA GATTGAGCTG TGGTTGGTGC 
     4021 TTGTGGCCGT GGGTGCTGGG TTGCTGCTGC TGGGGCTCAT CATCCTCCTC TTGTGGAAGT 
     4081 GCGGCTTCTT CAAGCGAGCC CGCACTCGTG CCCTGTATGA AGCTAAGAGG CAGAAGGCTG 
     4141 AGATGAAGAG CCAGCCGTCA GAGACAGAAA GGCTGACCGA CGACTACACG CGTACGCGGC 
     4201 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4261 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4321 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4381 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4441 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4501 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4561 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4621 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4681 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4741 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4801 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4861 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4921 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4981 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5041 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5101 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5161 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5221 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5281 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5341 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5401 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5461 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5521 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5581 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5641 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5701 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5761 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5821 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5881 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5941 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6001 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6061 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6121 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6181 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6241 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6301 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6361 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6421 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6481 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6541 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6601 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6661 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6721 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6781 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6841 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6901 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6961 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7021 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7081 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7141 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7201 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7261 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7321 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7381 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7441 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7501 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7561 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7621 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7681 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7741 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7801 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7861 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7921 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7981 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//