LOCUS       MR211495                7989 bp    DNA     circular  SYN 
DEFINITION  Pld1 (Myc-DDK-tagged) - Mouse phospholipase D1 (Pld1), transcript variant 1
ACCESSION   NM_001164056
VERSION     NM_001164056.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4146..4237 
                     /label="Myc-DDK"  
     misc_feature    4267..4852 
                     /label="PolyA signal"  
     misc_feature    4324..4343 
                     /label="Restriction Enzyme"  
     misc_feature    5128..5800 
                     /label="ColE1"  
     misc_feature    6230..7024 
                     /label="Kan/Neo"  
     misc_feature    7059..7361 
                     /label="SV40 ori"  
     misc_feature    7447..7924 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4128..4134 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4128 
                     /label="Pld1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCACTGAAA AGCGAGACCA GGGTGAACAC GTCTACACTG CAGAAAATCG 
     1081 CCGCAGATAT GAGCAACCTG ATAGAAAATC TCGACACCCG GGAGCTTCAT TTTGAGGGAG 
     1141 AGGAGGTGGA ATATGACGCA TCTCCAGGAG ACCCCAAGGC CCAGGAAGGG TGCATCCCAT 
     1201 TCTCTTCTAT TTACAACACT CAAGGATTTA AGGAGCCCAA CATCCAGACC TACCTCTCTG 
     1261 GCTGTCCCAT AAAAGCACAA GTACTGGAAG TGGAGCGCTT CACATCGACC TCAAGGGTGC 
     1321 CAAGTATCAA CCTGTACACG ATCGAGTTAA CGCACGGGGA ATTCACATGG CAAGTTAAGA 
     1381 GGAAATTCAA GCACTTCCAA GAGTTTCACA GAGAGCTGCT CAAGTACAAA GCCTTCATCC 
     1441 GGATCCCCAT CCCCACTAAA AGACATACAT TTAGAAGACA AAATGTCAAA GAAGAACCCC 
     1501 GAGAGATGCC CAGTCTGCCC CGTTCATCAG AAAACGCAAT CCAGGAAGAG CAGTTCTTTG 
     1561 GCAGGAGGAA GCAACTGGAA GATTACTTGA CAAAAATACT AAAAATGCCC ATGTACAGAA 
     1621 ACTATCATGC CACGACAGAG TTCCTTGATG TAAGCCAGCT GTCCTTCATC CATGATCTGG 
     1681 GACCAAAGGG CCTGGAAGGA ATGATCATGA AAAGGTCTGG GGGACACAGG ATACCAGGTG 
     1741 TGAATTGCTG TGGCCATGGA CGAGCCTGCT ACCGGTGGTC AAAGAGGTGG TTGATAGTAA 
     1801 AAGACTCTTT CCTGCTCTAT ATGAAACCAG ATAGCGGGGC TATTGCCTTC GTCCTCCTTG 
     1861 TAGATAAAGA ATTCAGAGTC AAAGTGGGAA GGAAAGAGAC AGAAACGAAA TATGGACTTC 
     1921 GGATCGATAA TCTTTCAAGG ACCCTGATTT TAAAATGTAA CAGCTACAGA CACGCCCGGT 
     1981 GGTGGGGAGG CGCCATCGAG GAGTTCATCC GGAAGCATGG AGCTGACTTC CTCAAAGACC 
     2041 ATCGCTTCGG GTCCTACGCC GCTCTCCATG AGAACACGTT AGCTAAGTGG TATGTGAACG 
     2101 CCAAAGGTTA CTTTGAAGAC ATCGCAAATG CAATGGAAGA GGCATCGGAA GAGATTTTTA 
     2161 TCACAGATTG GTGGTTAAGT CCAGAAATCT TCCTGAAGCG CCCAGTGGTG GAAGGCAACC 
     2221 GCTGGAGGCT GGACTGCATC CTCAAACGGA AAGCACAACA AGGTGTGCGG ATCTTCATAA 
     2281 TGCTTTACAA AGAGGTGGAG CTAGCCCTCG GGATCAACAG CGAGTACAGC AAGCGGACGT 
     2341 TGATGAGGCT GCACCCCAAC ATAAAGGTGA TGAGACACCC AGACCACGTG TCCTCCAGCG 
     2401 TGTACCTGTG GGCTCACCAT GAGAAGCTTG TCATTATCGA CCAATCAGTG GCTTTCGTGG 
     2461 GTGGGATTGA CCTGGCCTAC GGAAGGTGGG ATGACAATGA GCACAGACTC ACAGACGTGG 
     2521 GCAGTGTCAA GCGGGTCACC TCAGGACTGT CTCTGGGCTC CCTCACGGCA GCATCAGTAG 
     2581 AGTCTATGGA ATCCTTAAGC CTCAAGGATA AACATGAATT TCATAAAAAG GAGCCCATCT 
     2641 CAAAGATTGT TGATGAGACT GACATGAAGC TGAAAGGGAT AGGAAAGTCC AGGAAATTCT 
     2701 CCAAATTTAG TCTCTATCGC CAGCTGCACC GTCACCACCT GCACAACGCG GACAGCATCA 
     2761 GCAGCATCGA CAGCACCTCC AACACTGGCT CCATCCGCAG CGTGCAGACC GGAGTGGGAG 
     2821 AACTCCACGG GGAGACTAGG TTTTGGCATG GGAAGGATTA CTGCAACTTT GTCTTCAAGG 
     2881 ACTGGGTTCA ACTGGACAAA CCTTTTGCTG ATTTCATTGA CAGGTACTCA ACCCCCCGGA 
     2941 TGCCTTGGCA TGATATTGGC TCTGTGGTCC ATGGAAAGGC AGCTCGGGAT GTGGCTCGAC 
     3001 ACTTCATCCA GCGCTGGAAC TTCACGAAGA TTATGAAGCC AAAATACCGC TCCCTTTCTT 
     3061 ATCCCTTCCT GCTACCAAAG TCTCAAGCGA CAGCACACGA GTTGAGATAT CAGGTGCCAG 
     3121 GGGCCGTCCC CGCCAAAGTG CAGTTGCTCC GATCTGCTGC TGATTGGTCT GCTGGTATAA 
     3181 AGCACCATGA GGAATCCATC CACGCTGCCT ACATCCATGT AATAGAGAAC AGCAAGCACT 
     3241 ACATCTATAT TGAAAACCAA TTTTTCATAA GCTGTGCTGA TGATAAAGTT GTGTTCAACA 
     3301 AGGTGGGAGA TGCTATCGCC CAGAGAATCC TGAAAGCCCA CAGGGAAGGC CAGAGATATC 
     3361 GGGTGTACAT TGTGATACCG CTGCTGCCGG GATTCGAGGG AGACATTTCC ACAGGCGGGG 
     3421 GGAACGCTCT ACAGGCAATA ATGCACTTCA ACTACAGAAC CATGTGCAGA GGGGAGAGCT 
     3481 CCATCCTTGA ACAGTTAAAG CCAGAGCTTG GTAATAAGTG GATAAATTAC ATATCGTTTT 
     3541 GTGGACTGAG AACACATGCA GAGCTGGAAG GAAACCTAGT CACCGAGCTC ATCTACGTCC 
     3601 ACAGCAAGTT GTTAATTGCT GACGACAACA CAGTTATTAT TGGTTCTGCC AACATCAATG 
     3661 ACCGGAGCAT GCTGGGGAAG CGAGACAGCG AAATGGCTGT CATAGTGCAG GACACAGAGA 
     3721 CGGTCCCATC GGTGATGGAT GGAAAGGAAT ATCAGGCTGG TCGCTTTGCC CGAGACCTTC 
     3781 GCCTGGAGTG CTTCAGACTT GTCCTGGGTT ATCTTTCTGA CCCAAGTGAG GACCTTCAGG 
     3841 ATCCTGTGAG TGACAAATTC TTCAAGGAGA TTTGGGTTTC AACAGCAGCT CGAAACGCTA 
     3901 CCATATATGA TAAGGTCTTC CGGTGCCTTC CCAATGATGA AGTACACAAT TTAATCCAGC 
     3961 TGCGGGACTT TATAAACAAG CCCATATTAG CAAAAGAGGA TCGCCTTCGC GCCGAGGAAG 
     4021 AACTGAGGAA GATCCGCGGG TTCTTGGTGC AGTTCCCTCT CTATTTCTTA TCAGAAGAGA 
     4081 ACCTGCTGCC TTCTGTTGGA ACCAAAGAAG CCATTGTGCC TATGGAGGTT TGGACTACGC 
     4141 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4201 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4261 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4321 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4381 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4441 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4501 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4561 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4621 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4681 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4741 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4801 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4861 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4921 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4981 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5041 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5101 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5161 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5221 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5281 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5341 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5401 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5461 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5521 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5581 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5641 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5701 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5761 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5821 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5881 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5941 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6001 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6061 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6121 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6181 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6241 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6301 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6361 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6421 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6481 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6541 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6601 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6661 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6721 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6781 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6841 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6901 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6961 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7021 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7081 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7141 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7201 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7261 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7321 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7381 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7441 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7501 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7561 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7621 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7681 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7741 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7801 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7861 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7921 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7981 GCGAATTTT
//