LOCUS       MR211470                7953 bp    DNA     circular  SYN 
DEFINITION  Nat10 (Myc-DDK-tagged) - Mouse N-acetyltransferase 10 (Nat10)
ACCESSION   NM_153126
VERSION     NM_153126.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4110..4201 
                     /label="Myc-DDK"  
     misc_feature    4231..4816 
                     /label="PolyA signal"  
     misc_feature    4288..4307 
                     /label="Restriction Enzyme"  
     misc_feature    5092..5764 
                     /label="ColE1"  
     misc_feature    6194..6988 
                     /label="Kan/Neo"  
     misc_feature    7023..7325 
                     /label="SV40 ori"  
     misc_feature    7411..7888 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4092..4098 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4092 
                     /label="Nat10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATCGGAAG AAGGTGGATA ACCGAATTCG AATTCTCATT GAGAATGGCG 
     1081 TAGCTGAGCG GCAGAGGTCT CTTTTTGTTG TAGTTGGGGA TCGAGGAAAA GATCAGGTGG 
     1141 TTATTCTTCA TCATATGTTG TCCAAGGCAA CTGTGAAGGC TCGGCCCTCA GTCCTGTGGT 
     1201 GTTATAAGAA AGAGCTGGGA TTTAGCAGTC ACCGGAAGAA GAGGATGCGG CAGCTACAGA 
     1261 AGAAAATAAA GAGTGGGACC TTGAACTTAA AGCAAGATGA CCCCTTTGAG CTTTTTGTAG 
     1321 CAGCCACAAA CATTCGCTAC TGCTACTACA ATGAAACCCA CAAGATTCTG GGCAATACTT 
     1381 TCGGCATGTG TGTCCTCCAG GATTTTGAAG CGTTAACTCC GAACTTGTTG GCCAGAACTG 
     1441 TAGAAACAGT AGAAGGTGGT GGACTGGTGG TCATCCTCCT GCGGACCATG AACTCGCTTA 
     1501 AGCAGCTGTA CACGATGACT ATGGATGTGC ATTCCAGGTA CAGGACTGAG GCCCATCAGG 
     1561 ACGTGGTGGG AAGATTTAAC GAGAGGTTTA TTCTCTCTCT GGCCTCTTGT AAGAAGTGTC 
     1621 TGGTCATTGA CGATCAGCTC GACATCCTGC CCATCTCCTC CCACGTGGCC AGCATTGAAG 
     1681 CCTTACCTCC TCAGGCCCCG GATGAGAATC TCAGTCCTGC TGCTCTGGAG CTGCTGGAGT 
     1741 TGAAAGAGAG CTTGCAGGAC ACTCAGCCCG TGGGTGTTCT GGTGGACTGC TGCAAGACCC 
     1801 TGGACCAGGC CAAAGCTGTC TTGAAATTCA TTGAGGGGAT CTCGGAGAAG ACTCTAAGGA 
     1861 GTACTGTAGC CCTCACCGCT GCCCGAGGAA GGGGCAAGTC TGCGGCCCTG GGCCTGGCTA 
     1921 TCGCTGGAGC AGTGGCATTC GGGTATTCCA ATATTTTTGT TACCTCCCCA AGCCCGGATA 
     1981 ACCTCCACAC GCTGTTTGAA TTTGTATTTA AAGGATTTGA TGCTCTGCAG TATCAGGAGC 
     2041 ATCTGGATTA TGAGATTGTA CAGTCGCTGA ACCCCGAGTT TAATAAAGCG GTGATCAGGG 
     2101 TCAATGTGTT CCGAGAGCAC AGACAGACTA TTCAGTACAT CCACCCTGCA GATGCTGTGA 
     2161 AACTGGGCCA GGCTGAGCTG GTTGTGATAG ATGAAGCTGC CGCTATTCCC CTCCCCCTGG 
     2221 TGAAGAGCCT GCTTGGGCCC TACCTGGTTT TCATGGCATC TACTATCAAT GGCTACGAGG 
     2281 GCACTGGCCG GTCACTGTCC CTCAAGCTCA TTCAACAACT CCGTCAGCAG AGTGCCCAGA 
     2341 GCCAGGTCAG CACCACTGCT GAGAACAAGA CCACAACGAC AGCCAGACTG GCCTCAGCTC 
     2401 GAACCTTGCA TGAGGTTTCC CTCCAAGAGT CAATCCGATA CGCCCCTGGG GATGCAGTGG 
     2461 AGAAGTGGCT TAATGACCTG CTGTGCCTGG ATTGCCTCAA CATCACCCGC ATCGTTTCCG 
     2521 GCTGCCCCTT GCCTGAGGCC TGTGAGCTCT ACTATGTTAA CAGAGATACC CTCTTTTGCT 
     2581 ACCACAAGGC CTCTGAAGTT TTCCTCCAGC GGCTCATGGC TCTCTATGTG GCTTCTCATT 
     2641 ACAAGAACTC TCCCAACGAC CTGCAGATGC TCTCAGACGC TCCTGCTCAC CACCTCTTCT 
     2701 GCCTCCTGCC ACCTGTGCCC CCCACCCAGA ATGCCCTGCC TGAAGTGCTT GCAGTTGTCC 
     2761 AGGTGTGCCT TGAGGGAGAG ATTTCTCGTC AGTCCATTTT GAATAGCCTC TCTCGAGGCA 
     2821 AGAAGGCTTC TGGGGACCTG ATTCCATGGA CAGTGTCGGA ACAGTTTCAA GATCCAGACT 
     2881 TTGGAGGCCT TTCTGGTGGA CGGGTTGTTC GAATTGCTGT TCACCCAGAT TATCAAGGGA 
     2941 TGGGATATGG CAGCCGAGCG CTGCAGCTGC TGCAGATGTA TTATGAAGGC AAGTTCCCTT 
     3001 GTCTGGAGGA AAAAGTCTTG GAGACACCAC AAGAAATCCG CACCGTGAGC AGTGAGGCTG 
     3061 TCAGCTTGTT GGAAGAAGTT ATTACTCCCC GGAAGGATCT GCCCCCCTTG CTCCTCAAGT 
     3121 TGAATGAGAG GCCTGCAGAG CGCCTGGATT ACCTGGGGGT TTCCTATGGG CTGACCCCCA 
     3181 GGCTTCTCAA GTTCTGGAAA CGAGCTGGAT TTGTTCCTGT CTATTTGAGA CAGACCCCAA 
     3241 ACGACCTGAC TGGGGAACAC TCGTGCATTA TGCTGAAGAC GCTGGCCGAT GAGGATGAGG 
     3301 CTGAGCAGGG AGCTTGGCTG GCAGCATTTT GGAAAGATTT CCGAAGACGG TTCCTGGCCT 
     3361 TGCTGTCATA CCAGTTCAGC ACCTTCTCTC CTGCCCTGTC TCTGAACATC ATTCAGAACA 
     3421 GGAACGTAGC CAAGTCAGCC CTGCCAGCCC TAGGCCGGGA GCATCTGGAG GCACTTTTCC 
     3481 TTCCCTATGA CCTGAAGCGT CTAGAGATGT ACTCTCGGAA CATGGTCGAT TACCACCTCA 
     3541 TCATGGATCT TATCCCAGCC ATTTCCCGCT TGTACTTCCT GAACCAGCTA GGAGACCTGT 
     3601 CCCTGTCGGC TGCCCAGTCG GCTCTTCTCT TGGGAATTGG CCTACAGCAC AAGTCTGTGG 
     3661 ATCAGCTGGA AAAGGAGATT GAGCTGCCCT CAGGCCAGTT GATGGGGCTT TTCAACCGGA 
     3721 TCATCCGCAA AGTTGTGAAG CTGTTCAATG ATGTGCAGGA AAAGGCTATT GAGGAGCAGA 
     3781 TGGTGGCAGT GAAGGATGTG GTCATGGAGC CCACTATGAA GACCCTGAGT GATGACCTGG 
     3841 ATGAAGCAGC AAAGGAATTC CAGGAGAAAC ACAAGAAGGA AGTCGGGAAG CTGAAGGACA 
     3901 TGGACCTCTC TCAATATGTA ATTCGTGGGG ACGATGAAGA GTGGAATGAA GTTTTGAGCA 
     3961 AAGCAGGGCA GAATGCCTCC ATTGTTAGTT TGAAAAGTGA CAAGAAAAGG AAACTGGAAA 
     4021 CAAAACAAGA ACCCAAACAG AGCAAGAAGT TGAAGAAGAG AGATAACAAC AGAAAGGATA 
     4081 TGAAACTGAA GCGAAAGAAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4141 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4201 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4261 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4321 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4381 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4441 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4501 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4561 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4621 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4681 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4741 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4801 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4861 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4921 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4981 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5041 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5101 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5161 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5221 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5281 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5341 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5401 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5461 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5521 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5581 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5641 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5701 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5761 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5821 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5881 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5941 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6001 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6061 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6121 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6181 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6241 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6301 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6361 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6421 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6481 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6541 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6601 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6661 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6721 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6781 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6841 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6901 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6961 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7021 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7081 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7141 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7201 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7261 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7321 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7381 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7441 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7501 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7561 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7621 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7681 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7741 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7801 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7861 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7921 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//