LOCUS       MR211444                7914 bp    DNA     circular  SYN 
DEFINITION  Man2b1 (Myc-DDK-tagged) - Mouse mannosidase 2, alpha B1 (Man2b1)
ACCESSION   NM_010764
VERSION     NM_010764.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4069..4160 
                     /label="Myc-DDK"  
     misc_feature    4190..4775 
                     /label="PolyA signal"  
     misc_feature    4247..4266 
                     /label="Restriction Enzyme"  
     misc_feature    5051..5723 
                     /label="ColE1"  
     misc_feature    6153..6947 
                     /label="Kan/Neo"  
     misc_feature    6982..7284 
                     /label="SV40 ori"  
     misc_feature    7370..7847 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4059..4067 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4059 
                     /label="Man2b1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCACCGGA CCGCTCACCT CTGGTGTCCG TGCTGGTGGC GGGAACACCG 
     1081 GGTGGCTCTG GATGAGCTCC TGCAACCTAG GTTCGCCAGT CCTGCCTATT TCCTTTCTTT 
     1141 TTTGGCTGTT GCTGGCCGCG CCTGGCGCTC GGGCCGCGGG TTACAAGACA TGTCCCCCAA 
     1201 CGAAGCCAGG CATGCTCAAT GTGCACCTGC TGCCTCATAC ACACGATGAT GTAGGCTGGC 
     1261 TGAAGACAGT GGACCAATAC TATTACGGCA TCCTGAGTGA TGTTCAGCAC GCATCTGTTC 
     1321 AGTACATCCT AGACTCAGTA GTCTCCTCTC TACTGGAGAA ACCCACCCGT CGCTTCATCT 
     1381 ATGTGGAAAT GGCTTTCTTC TCCCGTTGGT GGAAGCAGCA GACCAGTGCA ACCCAGGACG 
     1441 CTGTACGGAA CCTGGTGCGC CAGGGGCGCC TGGAGTTTGT CAATGGTGGC TGGGTGATGA 
     1501 ACGACGAAGC AGCCACCCAC TATGGCGCGA TTGTGGACCA GATGACACTT GGGCTACGCT 
     1561 TCCTGCAAGA CACATTTGGT AGTGATGGAC TTCCCCGTGT GGCCTGGCAT ATTGACCCTT 
     1621 TCGGCCACTC TCGAGAACAG GCCTCCCTGT TTGCCCAGAT GGGTTTTGAT GGCTTCTTCC 
     1681 TTGGGCGCAT TGACTATCAA GATAAACTTA ATCGGAAGAA AAAGCTGAGG ATGGAGGAGC 
     1741 TGTGGAGAGC TAGTGACAGC TTGGAGCCCC CCGCTGCTGA CCTCTTTACT GGTGTGCTCC 
     1801 CCAATAATTA CAACCCCCCG AAATACCTGT GCTGGGATGT GCTATGTACA GACCCTCCCG 
     1861 TGGTGGATAA TCCCCGGAGC CCTGAGTTCA ATGCAAAAAC GTTGGTCAAC TACTTCCTGA 
     1921 AGCTCGCTTC TTCCCAGAAA GGGTTTTACC GAACCAACCA CACCGTGATG ACCATGGGCT 
     1981 CAGACTTCCA TTATGAGAAT GCCAACATGT GGTTCAAAAA CATGGACAAG CTGATCCGCC 
     2041 TGGTCAACGC GCAGCAGGTA AACGGGAGCC TCGTCCACGT TCTCTACTCC ACCCCTACGT 
     2101 GTTACCTCTG GGAGCTGAAC AAGGCCAATC TCACCTGGAC AGTGAAGGAG GACGACTTCT 
     2161 TTCCTTATGC CGATGGCCCC CACATGTTCT GGACTGGCTA TTTTTCCAGC AGGCCAGCCC 
     2221 TCAAGCGCTA TGAGCGCCTC AGTTACAACT TCCTGCAGGT GTGTAACCAG CTGGAAGCAC 
     2281 TGGTGGGTCC CGAAGCCAAC GTGGGACCCT ATGGCTCGGG AGACAGCGCA CCTCTTCAGG 
     2341 AGGCAATGGC GGTGCTCCAA CACCACGACG CTGTCAGTGG TACTGCAAGG CAGAATGTTG 
     2401 TAAATGACTA CGCGAGGCAG CTGGCAGCAG GCTGGGGACC CTGTGAGGTT CTCGTGAGCA 
     2461 ACGCTCTGGC GCGACTCAGC CATTACAAAC AGAACTTCTC ATTCTGTCGT GAGCTCAACA 
     2521 TTAGCATCTG CCCAGTCAGC CAGACTTCGG AGCGTTTCCA GGTAACTCTT TACAACCCCC 
     2581 TGGGGCGAAA GGTGGATCAG ATGGTTCGGC TGCCTGTCTA TGAAGGCAAC TTCATTGTGA 
     2641 AGGACCCTCA TGATAAGAAC ATTTCCAGTA ACGTGGTGAT GGTTCCTAGC TATTACAGTG 
     2701 AGACTTACCA ATGGGAGCTG CTGTTCCCAG CCTCAGTGCC TGCCCTGGGC TTCAGCACCT 
     2761 ACTCTGTGGC CAAAATGAGT GACCTTAACC ACCAAGCCCA CAACCTGCTG TCCAGGCCCA 
     2821 GGAAACACAA GTCCCATCAT GTCTTAGTCA TTGAAAATAA GTACATGCGG GCCACATTTG 
     2881 ACTCTGGCAC GGGGTTATTG ATGAAGATTG AAAACTTGGA ACAGAACCTC TCGCTCCCTG 
     2941 TGAGCCAAGG CTTCTTTTGG TATAATGCCA GTGTTGGTGA TGAGGAGAGT TCTCAGGCCT 
     3001 CTGGGGCCTA CATCTTCAGA CCCAATGTCG GTAAACCGAT ACCTGTGAGC CGCTGGGCTC 
     3061 AGATCAGTTT GGTGAAGACA GCCTTGGTGC AGGAGGTACA CCAGAATTTT TCAGCCTGGT 
     3121 GTTCCCAAGT GATTCGCCTG TACAAAGGAC AGCGCCATTT GGAGCTGGAG TGGACGGTGG 
     3181 GACCAATCCC AGTGAGAGAT GACTGGGGGA AGGAAGTCAT CAGCCGCTTT GACACCCCAA 
     3241 TGAAAACCAA GGGACAGTTC TTCACGGACA GCAACGGCCG GGAAATCCTG AAGAGGAGGG 
     3301 ATGATTATCG ACCCACCTGG ACATTGAATC AGACTGAGCC GGTGGCTGGA AATTACTATC 
     3361 CTGTCAACAC TCGAATTTAT ATCACGGATG GGCAAATGCA GCTGACCGTG TTGACCGACC 
     3421 GCTCCCAGGG GGGCAGCAGT CTGCAGGATG GCTCGTTGGA GCTCATGGTG CACCGTCGGC 
     3481 TGTTGGTAGA TGATGATCGT GGAGTGTCGG AACCCTTGTT GGAGACAGAT ACCGGGGATA 
     3541 AGGTTCGAGG ACGCCACCTT GTGCTTTTGA GTTCTGTCAG TGATGCCGCT GCAAGACACC 
     3601 GACTGCTGGC GGAACAAGAA GTCCTGGCCC CTCAGGTGGT GCTGTCTCTG GGTGGTAGCA 
     3661 GTCCCTATCA CTCCCGGGCG ACACCAAAAA CGCAGTTTTC CGGACTTCGC CAGGAGCTAC 
     3721 CTCCTCAGGT GCATTTGCTC ACACTGGCCC GCTGGGGGCC AAAGATGCTG CTGCTGCGCT 
     3781 TGGAGCACCA GTTCGCTCTG AAAGAAGATT CGGATCGCAA CCTGAGCTCC CCTGTAACCT 
     3841 TGAACGTTCA GAACCTGTTC CAGACCTTCA CTATCAACTA CCTGCAGGAG ACCACACTGG 
     3901 CAGCCAACCA GCCCCTGTCC AGGGCTTCCA GGCTCAAGTG GATGACAAAT ACTGGTCCCA 
     3961 CCTCCTACCC TGAGCCTTCC AAGTTGGACC CTACCTCAGT CACACTGAAG CCTATGGAAA 
     4021 TACGTACCTT CCTGGCCTCT GTACAATGGC AAGAACACAG GCCTGCCACG CGGCCGCTCG 
     4081 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4141 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4201 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4261 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4321 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4381 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4441 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4501 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4561 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4621 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4681 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4741 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4801 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4861 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4921 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4981 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5041 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5101 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5161 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5221 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5281 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5341 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5401 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5461 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5521 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5581 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5641 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5701 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5761 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5821 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5881 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5941 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6001 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6061 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6121 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6181 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6241 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6301 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6361 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6421 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6481 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6541 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6601 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6661 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6721 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6781 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6841 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6901 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6961 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7021 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7081 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7141 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7201 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7261 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7321 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7381 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7441 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7501 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7561 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7621 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7681 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7741 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7801 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7861 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//