LOCUS       MR211390                7842 bp    DNA     circular  SYN 
DEFINITION  Ephb4 (Myc-DDK-tagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 2
ACCESSION   NM_010144
VERSION     NM_010144.6
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3999..4090 
                     /label="Myc-DDK"  
     misc_feature    4120..4705 
                     /label="PolyA signal"  
     misc_feature    4177..4196 
                     /label="Restriction Enzyme"  
     misc_feature    4981..5653 
                     /label="ColE1"  
     misc_feature    6083..6877 
                     /label="Kan/Neo"  
     misc_feature    6912..7214 
                     /label="SV40 ori"  
     misc_feature    7300..7777 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3981..3987 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3981 
                     /label="Ephb4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTCCGA GCGCTGCTGT GCTGGGCTTC CCTCGCCACT GCTTTAGAAG 
     1081 AGACCCTGTT GAACACAAAA CTGGAAACGG CGGATCTGAA ATGGGTGACT TACCCTCAGG 
     1141 CAGAGGGCCA GTGGGAGGAG CTAAGCGGCC TGGATGAGGA ACAGCACAGC GTCCGCACCT 
     1201 ATGAGGTGTG CGACATGAAG CGTCCAGGGG GCCAGGCTCA CTGGCTGCGC ACTGGCTGGG 
     1261 TCCCAAGGCG AGGTGCTGTC CACGTGTATG CCACGATACG CTTCACCATG ATGGAATGCC 
     1321 TGTCCCTGCC GAGGGCCAGT CGCTCCTGCA AGGAGACATT CACTGTCTTC TATTACGAGA 
     1381 GCGAAGCTGA TACGGCCACG GCCCATACGC CCGCCTGGAT GGAGAACCCC TACATCAAGG 
     1441 TGGACACAGT GGCCGCAGAA CATCTGACTC GGAAGCGCCC TGGAGCTGAA GCCACAGGGA 
     1501 AAGTTAATAT CAAGACGCTG CGCCTGGGTC CTCTCAGCAA AGCTGGCTTC TACCTGGCTT 
     1561 TCCAGGACCA AGGAGCCTGC ATGGCTCTGC TCTCCCTGCA TCTCTTTTAC AAGAAGTGCT 
     1621 CCTGGCTGAT CACGAACTTG ACCTACTTCC CCGAGACGGT GCCTCGGGAG CTCGTGGTGC 
     1681 CGGTGGCAGG TAGCTGCGTG GCCAACGCGG TCCCTACCGC CAACCCCAGC CCCAGCCTCT 
     1741 ACTGCCGGGA AGATGGTCAA TGGGCTGAGC AGCAGGTCAC GGGCTGCAGC TGCGCGCCAG 
     1801 GGTACGAGGC TGCGGAAAGC AACAAAGTAT GCAGAGCCTG TGGCCAGGGA ACCTTCAAGC 
     1861 CCCAAATAGG AGACGAGTCC TGCCTGCCAT GCCCAGCCAA CAGCCACTCG AATAACATTG 
     1921 GGTCTCCTGT CTGCCTGTGT CGAATTGGGT ATTACCGGGC CCGCTCAGAC CCCCGGAGTT 
     1981 CACCTTGCAC TACCCCACCC TCTGCTCCAA GAAGCGTGGT TCACCATTTG AATGGTTCCA 
     2041 CCCTGCGCCT GGAATGGAGT GCTCCCCTTG AGTCCGGAGG CCGAGAGGAC CTCACTTATG 
     2101 CTGTACGCTG CCGAGAGTGC CGTCCTGGGG GTTCCTGCTT GCCCTGTGGG GGCGACATGA 
     2161 CCTTCGACCC CGGTCCTCGA GACCTGGTTG AGCCCTGGGT GGCAATCCGA GGGCTGCGTC 
     2221 CTGATGTCAC CTATACCTTT GAGGTTGCTG CTTTGAATGG TGTGTCTACC TTAGCCACTG 
     2281 GACCACCTCC TTTTGAGCCT GTCAATGTCA CCACTGACCG TGAGGTGCCT CCTGCAGTGT 
     2341 CTGACATCCG AGTGACTCGG TCGTCACCCA GCAGCTTGAT CCTGTCATGG GCTATCCCCA 
     2401 GAGCACCCAG TGGGGCCGTG CTGGACTACG AGGTCAAGTA TCATGAGAAG GGCGCAGAGG 
     2461 GCCCCAGCAG TGTTCGTTTC CTGAAGACAT CAGAAAACCG AGCTGAGCTC CGGGGGCTGA 
     2521 AGCGGGGAGC CAGCTATCTG GTCCAGGTAC GCGCACGGTC CGAGGCTGGC TACGGTCCCT 
     2581 TCGGCCAGGA GCATCACAGT CAGACTCAAC TGGATGAGAG CGAGAGCTGG CGGGAGCAGC 
     2641 TGGCCCTGAT TGCAGGCACT GCGGTTGTGG GTGTGGTCCT GGTCCTGGTG GTCGTCATCA 
     2701 TTGCAGTTCT CTGCCTCAGG AAGCAGAGCA ATGGGAGGGA AGTTGAGTAC TCGGATAAGC 
     2761 ATGGGCAGTA TCTCATCGGG CACGGTACCA AGGTCTACAT TGATCCTTTT ACTTACGAAG 
     2821 ACCCTAATGA GGCAGTGAGG GAATTTGCCA AAGAGATCGA TGTCTCCTAT GTCAAGATTG 
     2881 AAGAGGTAAT TGGTGCAGGT GAGTTCGGCG AGGTGTGCCG GGGTCGGCTG AAGGCACCAG 
     2941 GGAAAAAGGA GAGCTGTGTG GCCATCAAGA CTCTGAAGGG TGGCTACACC GAGCGCCAGA 
     3001 GGCGTGAGTT CCTGAGCGAG GCCTCCATCA TGGGCCAGTT CGAGCATCCC AACATCATCC 
     3061 GCCTCGAGGG CGTGGTCACC AACAGTGTGC CGGTTATGAT CCTCACGGAA TTCATGGAGA 
     3121 ACGGAGCCCT GGACTCCTTC CTGCGGCTGA ACGACGGGCA GTTCACAGTC ATCCAGCTGG 
     3181 TGGGCATGCT GAGGGGCATC GCCTCGGGCA TGCGGTACCT GGCTGAAATG AGCTATGTCC 
     3241 ACCGAGACCT GGCTGCTCGG AACATCTTGG TCAACAGTAA CCTGGTCTGC AAGGTGTCCG 
     3301 ACTTTGGCCT CTCCAGATTC TTGGAGGAGA ACTCCTCTGA TCCCACCTAC ACAAGTTCCC 
     3361 TGGGAGGCAA GATTCCCATC CGATGGACCG CCCCTGAAGC CATTGCCTTC AGGAAGTTCA 
     3421 CCTCTGCCAG TGATGCCTGG AGCTATGGGA TCGTCATGTG GGAGGTCATG TCTTTTGGGG 
     3481 AACGGCCATA CTGGGACATG AGCAACCAGG ATGTGATCAA TGCCATTGAA CAGGACTACC 
     3541 GGCTGCCTCC TCCTCCAGAC TGCCCCACCT CCCTCCACCA GCTCATGCTG GATTGTTGGC 
     3601 AGAAGGACCG GAATGCCCGG CCCCGCTTTC CCCAGGTGGT CAGCGCTCTG GACAAGATGA 
     3661 TCCGGAATCC CGCTAGCCTC AAAATCGTGG CCAGGGAGAA TGGCGGGGCC TCACATCCAC 
     3721 TCTTGGACCA ACGGCAGCCT CACTATTCTG CTTTCGGTTC TGTGGGCGAG TGGCTTCGAG 
     3781 CCATCAAGAT GGGAAGATAC GAGGAAAGTT TTGCAGCGGC TGGATTCGGC TCCTTTGAGG 
     3841 TGGTCAGTCA GATCTCTGCC GAGGACCTTC TCCGAATTGG AGTCACTCTG GCAGGACACC 
     3901 AGAAGAAAAT CTTGGCCAGT GTGCAGCATA TGAAGTCCCA AGCTAAGCCA GGAGCCCCTG 
     3961 GTGGGACAGG GGGACCAGCC CAGCAGTTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4021 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4081 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4141 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4201 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4261 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4321 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4381 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4441 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4501 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4561 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4621 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4681 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4741 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4801 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4861 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4921 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4981 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5041 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5101 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5161 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5221 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5281 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5341 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5401 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5461 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5521 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5581 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5641 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5701 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5761 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5821 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5881 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5941 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6001 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6061 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6121 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6181 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6241 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6301 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6361 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6421 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6481 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6541 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6601 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6661 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6721 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6781 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6841 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6901 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6961 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7021 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7081 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7141 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7201 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7261 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7321 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7381 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7441 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7501 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7561 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7621 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7681 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7741 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7801 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//