LOCUS       MR211173                7644 bp    DNA     circular  SYN 
DEFINITION  Pcdhgb1 (Myc-DDK-tagged) - Mouse protocadherin gamma subfamily B, 1 (Pcdhgb1)
ACCESSION   NM_033574
VERSION     NM_033574.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3799..3890 
                     /label="Myc-DDK"  
     misc_feature    3920..4505 
                     /label="PolyA signal"  
     misc_feature    3977..3996 
                     /label="Restriction Enzyme"  
     misc_feature    4781..5453 
                     /label="ColE1"  
     misc_feature    5883..6677 
                     /label="Kan/Neo"  
     misc_feature    6712..7014 
                     /label="SV40 ori"  
     misc_feature    7100..7577 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3774..3781 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3774 
                     /label="Pcdhgb1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAAATCAG GTATTGCTTC ACTTCCTGGT GCCTTTGTTC TGCGGCGCCC 
     1081 TCTCCCAGCC CATCCACTAC TCCATTCCGG AGGAGCTAGC CAAGGGCTCT CTAGTGGGGA 
     1141 GTCTCGCCAA GGATCTGGGA CTCAGTATCC AAGAATTGCC AGCTCGAAAA CTGAGAGTTA 
     1201 GTGCAGAGGA TTATTTCAGC GTTAGTGCAG AGAGTGGGGA TTTGTTAGTG AGCGGCAGGA 
     1261 TAGACAGAGA AAAGATTTGT GGGAGGAAAT CTGAGTGTGC ACTGGAATTT GAATCTGTTA 
     1321 CTGAAAACCC GATGAACATT TTCCACGTAA CGGTTGCCAT CCAAGACATT AACGATAATG 
     1381 CACCACATTT CTTTGGGAAA AGCATTGAAT TGGAGATCTG TGAGTCAGCC TTAGCAGGGG 
     1441 CAAAATTCCC TCTGGACTCT GCGCGAGATG CAGATGTGGG AAGCAACTCC CTGAAGATGT 
     1501 ACACTGTCAG CCCCAATCCA CACTTCTCCT TGTCAACGAA GGAAAGTCCT GATGGAAGTA 
     1561 AATACCCAGA ACTGCTGCTG GAAACCCCTC TGGACAGGGA GCATCAGAGC TCCCATCACT 
     1621 TAATACTGAC CGCCATGGAT GGTGGAGACC CGCCCCTAAG CAGCACCACC CAGGTCTGGA 
     1681 TCAAAGTCAC CGACGCCAAT GACAATGCTC CAGTGTTCAG CCAGGACACT TACAAAGTTA 
     1741 GCCTTCGTGA AAACATACCC CCAGGAACGT TAGTGCTGCA AGTGACAGCT TCTGATCAGG 
     1801 ATGAGAGTAT TCACGGAGAG ATCACCTATG CCTTCCTTAA TGCCCCAGCA AGTACTAACC 
     1861 TTGTCTTCAA TCTCAATCCA AATACTGGAG CTATCACAAC TAATGGTACA TTGGATTTTG 
     1921 AAGAAAAGAA TAGATACACA TTGGGTGTAG AAGCCAAGGA CGGAGGGGTG CACACGGCTC 
     1981 ACTGTAATGT TCAGATCGAA ATTCTTGATG ACAACGACAA TGCCCCAGAG GTGACATTCA 
     2041 TGTCCTTTTC TAACCAAGTT CCAGAGGATT CAGTCCTTGG AACAGTAATC GCCCTCATTA 
     2101 AAGTTCGAGA CAAAGATTCT GGACAAAATG GTTTGGTGGC ATGCCATATT CAGGAAGAAC 
     2161 TTCCCTTCAA ATTACAACCC ACCTCCAAGA ATTATTACAA GTTAGTGATT GACAAGGCCC 
     2221 TAAACCGAGA ACAGACCCCA GAGTACAACG TCACTATCAC AGCTACTGAC AGTGGCAAGC 
     2281 CCTCGCTTTC CTCCAAGACA TGTGTTACTG TACACATCAC AGATATCAAC GACAATGCAC 
     2341 CGGTTTTTCA CCAGGCCTCC TACTTGGTCC ACGTGGCTGA GAACAACCCA CCTGGAGCCT 
     2401 CTATTGCTCA AGTCAGCGCC TCAGATCCGG ATTTGGGATC CAATGGCCTC ATTTCCTACT 
     2461 CCATCATAGC CAGCGACCTA GAGCCAAGGG CACTATCGTC CTTCGTGTCC GTGAACCAGG 
     2521 ACAGTGGAGT GGTGTTCGCG CAGCGCGCCT TCGACCACGA GCAGCTGCGT TCCTTCCAGC 
     2581 TGACACTGCA GGCGCGGGAC CACGGATCGC CCACACTCAG TGCCAATGTG AGCATGCGCG 
     2641 TGTTGGTGGG TGACCGCAAC GACAACGCAC CACGCGTGCT GTATCCCACA CTTGAGCCCG 
     2701 ACGGCTCTGC GCTCTTCGAC ATGGTGCCGC GCGCTGCCGA GCCCGGATAC CTGGTCACCA 
     2761 AGGTGGTGGC TGTGGACGCA GACTCCGGAC ACAATGCCTG GCTGTCTTAC CACGTGCTGC 
     2821 AGGCCAGCGA TCCCGGGCTC TTCAGCCTGG GACTGCGCAC AGGCGAGGTG CGCACAGCGC 
     2881 GTGCCCTGGG AGACAGGGAC TCAGCGCGGC AGCGCCTTCT GGTCGCTGTG CGTGATGGTG 
     2941 GACAGCCACC GCTCTCCGCT ACAGCCACGC TGCACCTCAT CTTCGCTGAC AGCCTGCAGG 
     3001 AGGTCCTGCC AGACCTCAGC GATGACCCTC TACCATCTGA CCCTCAGAGT GAGCTTCAGT 
     3061 TTTACCTGGT GGTGGCCTTG GCCCTGATCT CAGTGCTCTT CCTCCTGGCG GTGATTCTGG 
     3121 CTATTGCCCT GCGTCTGAGA CACTCCTCCA GATCAGCTGC CTGGGGCTGC TTTCAGCCTG 
     3181 GCCTCAGCCT GAAGTCTCAA CCTGTGGTTC CTCCCAACTA CAATGAGGGG ACATTACCCT 
     3241 ATTCCTATAA TCTCTGCGTT GCTTCACATT CTACGACTAT GGCAGGGTTT AATTTTCACC 
     3301 ACGTGGCACC AGAGGTTGCT CCCCCTCAGG ATCTCCTGTG CGATGATCCT TCTGTGGTTG 
     3361 TATGTGGCAA TAATGAAGAT CCCCAAGTCT CGGACGACTC TGCTTTCCAG CAAGCCCCGC 
     3421 CCAACACTGA CTGGCGTTTC TCTCAAGCCC AGAGACCCGG CACGAGCGGA TCCCAAAATG 
     3481 GTGATGAAAC TGGCACCTGG CCCAACAACC AGTTTGATAC AGAGATGCTG CAAGCCATGA 
     3541 TCTTGGCCTC TGCCAGCGAA GCTGCTGATG GGAGCTCTAC CCTGGGAGGG GGCGCTGGCA 
     3601 CCATGGGTCT GAGCGCTCGA TACGGACCCC AGTTTACCCT GCAGCACGTG CCTGACTACC 
     3661 GCCAGAACGT GTACATCCCT GGCAGCAATG CCACGCTGAC CAATGCCGCT GGCAAACGAG 
     3721 ATGGCAAGGC TCCAGCAGGT GGTAATGGCA ACAAGAAGAA ATCGGGCAAG AAAGAGAAGA 
     3781 AGAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3841 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3901 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3961 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4021 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4081 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4141 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4201 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4261 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4321 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4381 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4441 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4501 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4561 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4621 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4681 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4741 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4801 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4861 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4921 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4981 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5041 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5101 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5161 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5221 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5281 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5341 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5401 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5461 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5521 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5581 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5641 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5701 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5761 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5821 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5881 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5941 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6001 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6061 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6121 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6181 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6241 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6301 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6361 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6421 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6481 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6541 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6601 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6661 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6721 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6781 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6841 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6901 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6961 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7021 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7081 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7141 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7201 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7261 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7321 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7381 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7441 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7501 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7561 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7621 TAACAAAAAT TTAACGCGAA TTTT
//