LOCUS       MR211166                7626 bp    DNA     circular  SYN 
DEFINITION  Grm7 (Myc-DDK-tagged) - Mouse glutamate receptor, metabotropic 7 (Grm7)
ACCESSION   NM_177328
VERSION     NM_177328.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3783..3874 
                     /label="Myc-DDK"  
     misc_feature    3904..4489 
                     /label="PolyA signal"  
     misc_feature    3961..3980 
                     /label="Restriction Enzyme"  
     misc_feature    4765..5437 
                     /label="ColE1"  
     misc_feature    5867..6661 
                     /label="Kan/Neo"  
     misc_feature    6696..6998 
                     /label="SV40 ori"  
     misc_feature    7084..7561 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3765..3771 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3765 
                     /label="Grm7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCAGCTG GGGAAGCTGC TCCGCGTCCT GACTTTGATG AAGTTCCCCT 
     1081 GCTGCGTGCT GGAGGTGCTC CTGTGCGTGC TGGCGGCGGC GGCGCGCGGC CAGGAGATGT 
     1141 ACGCCCCGCA CTCGATCCGG ATCGAGGGGG ACGTCACCCT TGGGGGGCTG TTCCCAGTGC 
     1201 ACGCCAAGGG TCCCAGCGGA GTGCCCTGCG GCGACATCAA GAGGGAGAAT GGGATCCACA 
     1261 GGCTGGAAGC TATGCTTTAT GCTCTCGACC AGATCAACAG CGATCCCAAC CTGCTGCCCA 
     1321 ACGTAACGTT AGGCGCGCGG ATCCTGGACA CTTGTTCCAG GGACACTTAT GCGCTCGAAC 
     1381 AGTCGCTCAC TTTCGTCCAG GCGCTTATCC AGAAGGACAC CTCCGACGTG CGTTGCACCA 
     1441 ACGGAGAGCC CCCGGTTTTC GTCAAGCCAG AGAAAGTAGT TGGAGTGATT GGGGCTTCGG 
     1501 GGAGCTCCGT CTCCATCATG GTAGCCAACA TCTTGAGGCT TTTCCAGATA CCTCAGATTA 
     1561 GTTATGCATC CACGGCTCCT GAACTCAGTG ATGACCGTCG CTATGACTTC TTCTCTCGCG 
     1621 TGGTCCCACC TGATTCCTTC CAAGCCCAAG CTATGGTAGA CATTGTAAAG GCATTGGGCT 
     1681 GGAATTATGT ATCTACTCTT GCATCTGAAG GAAGCTATGG AGAGAAAGGT GTGGAGTCCT 
     1741 TCACACAAAT TTCCAAAGAA GCAGGTGGAC TCTGCATTGC CCAGTCCGTG AGAATTCCCC 
     1801 AAGAACGCAA AGACAGGACC ATTGACTTTG ATAGAATTAT CAAACAGCTC TTGGACACCC 
     1861 CCAACTCCAG GGCTGTCGTG ATTTTTGCCA ACGATGAGGA TATAAAGCAG ATCCTTGCAG 
     1921 CAGCCAAAAG AGCTGACCAA GTAGGACATT TTCTCTGGGT TGGGTCAGAC AGCTGGGGTT 
     1981 CCAAAATCAA CCCACTGCAT CAGCATGAAG ATATTGCAGA AGGAGCCATC ACCATCCAAC 
     2041 CTAAGAGAGC GACTGTGGAA GGATTTGATG CTTACTTCAC ATCCCGGACA CTTGAAAACA 
     2101 ACAGGAGAAA TGTATGGTTT GCCGAATACT GGGAAGAAAA CTTCAACTGC AAGTTGACAA 
     2161 TTAGTGGATC CAAAAAAGAA GACACGGATC GCAAATGCAC AGGACAGGAG CGAATTGGAA 
     2221 AAGACTCTAA TTATGAGCAG GAGGGTAAAG TACAGTTTGT GATCGATGCT GTCTATGCCA 
     2281 TGGCACATGC CCTTCATCAC ATGAATAAGG ATCTGTGTGC TGACTACCGG GGAGTGTGCC 
     2341 CAGAAATGGA GCAAGCAGGG GGCAAGAAGT TGTTGAAGTA TATCCGCAAT GTTAACTTCA 
     2401 ATGGTAGTGC TGGAACCCCA GTGATGTTTA ACAAAAATGG TGATGCTCCA GGACGATATG 
     2461 ACATCTTCCA GTACCAGACA ACAAACACTA CCAATCCTGG TTATCGTCTC ATTGGGCAGT 
     2521 GGACAGATGA ACTGCAGCTC AATATAGAGG ACATGCAGTG GGGCAAAGGA GTCCGGGAGA 
     2581 TCCCACCCTC TGTGTGCACA TTGCCATGCA AGCCTGGGCA GAGGAAGAAG ACACAGAAGG 
     2641 GAACCCCTTG CTGCTGGACT TGTGAGCCCT GTGATGGATA CCAGTATCAA TTTGATGAGA 
     2701 TGACATGTCA GCATTGTCCC TATGACCAGA GGCCCAATGA GAACCGAACT GGCTGTCAGA 
     2761 ACATCCCAAT CATCAAACTG GAGTGGCACT CCCCCTGGGC TGTAATTCCT GTCTTCCTGG 
     2821 CAATGTTGGG GATCATTGCC ACCATCTTTG TCATGGCAAC CTTCATCCGC TACAATGATA 
     2881 CACCCATCGT CAGGGCATCT GGGCGGGAAC TCAGCTATGT TTTATTGACA GGCATCTTTC 
     2941 TCTGCTATAT CATCACCTTC CTAATGATTG CCAAGCCAGA TGTGGCAGTG TGTTCCTTCC 
     3001 GACGTGTCTT CTTGGGGTTG GGTATGTGTA TTAGTTATGC AGCCCTTTTA ACAAAAACCA 
     3061 ATCGGATTTA TCGCATATTC GAGCAGGGCA AGAAATCGGT GACAGCTCCC AGACTCATAA 
     3121 GCCCAACATC ACAACTGGCA ATTACCTCCA GTTTAATATC GGTGCAACTT CTAGGTGTCT 
     3181 TTATTTGGTT TGGGGTTGAC CCACCCAACA TCATCATAGA CTACGATGAG CATAAGACAA 
     3241 TGAACCCAGA ACAAGCCAGG GGAGTTCTCA AATGTGACAT CACAGACCTT CAGATCATTT 
     3301 GTTCTTTGGG ATATAGCATT CTTCTCATGG TCACATGTAC TGTGTATGCC ATCAAGACTC 
     3361 GGGGTGTACC AGAGAATTTT AATGAAGCCA AGCCCATTGG ATTCACTATG TACACGACTT 
     3421 GTATCGTATG GCTTGCCTTC ATCCCAATAT TTTTTGGCAC AGCGCAATCA GCAGAAAAGC 
     3481 TCTACATACA AACCACCACG CTTACAATCT CCATGAACCT AAGTGCATCA GTGGCGCTGG 
     3541 GGATGCTATA CATGCCGAAA GTGTACATCA TCATTTTCCA CCCTGAACTC AATGTCCAGA 
     3601 AACGGAAGCG AAGCTTCAAG GCCGTAGTCA CAGCAGCCAC CATGTCATCG AGGCTGTCAC 
     3661 ACAAACCCAG TGACAGGCCC AATGGTGAGG CAAAGACAGA ACTTTGTGAA AATGTAGACC 
     3721 CAAACAGCCC TGCTGCAAAA AAGAAGTATG TCAGTTATAA TAACCTGGTT ATCACGCGTA 
     3781 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3841 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3901 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3961 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4021 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4081 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4141 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4201 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4261 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4321 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4381 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4441 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4501 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4561 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4621 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4681 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4741 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4801 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4861 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4921 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4981 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5041 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5101 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5161 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5221 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5281 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5341 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5401 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5461 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5521 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5581 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5641 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5701 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5761 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5821 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5881 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5941 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6001 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6061 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6121 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6181 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6241 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6301 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6361 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6421 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6481 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6541 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6601 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6661 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6721 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6781 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6841 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6901 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6961 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7021 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7081 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7141 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7201 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7261 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7321 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7381 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7441 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7501 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7561 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7621 AATTTT
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