LOCUS       MR211146                7605 bp    DNA     circular  SYN 
DEFINITION  DNMT3A (Myc-DDK-tagged) - Mouse DNA methyltransferase 3A (DNMT3A), transcript variant 1
ACCESSION   NM_007872
VERSION     NM_007872.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3762..3853 
                     /label="Myc-DDK"  
     misc_feature    3883..4468 
                     /label="PolyA signal"  
     misc_feature    3940..3959 
                     /label="Restriction Enzyme"  
     misc_feature    4744..5416 
                     /label="ColE1"  
     misc_feature    5846..6640 
                     /label="Kan/Neo"  
     misc_feature    6675..6977 
                     /label="SV40 ori"  
     misc_feature    7063..7540 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3744..3750 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3744 
                     /label="Dnmt3a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCTCCAGC GGCCCCGGGG ACACCAGCAG CTCCTCTCTG GAGCGGGAGG 
     1081 ATGATCGAAA GGAAGGAGAG GAACAGGAGG AGAACCGTGG CAAGGAAGAG CGCCAGGAGC 
     1141 CCAGCGCCAC GGCCCGGAAG GTGGGGAGGC CTGGCCGGAA GCGCAAGCAC CCACCGGTGG 
     1201 AAAGCAGTGA CACCCCCAAG GACCCAGCAG TGACCACCAA GTCTCAGCCC ATGGCCCAGG 
     1261 ACTCTGGCCC CTCAGATCTG CTACCCAATG GAGACTTGGA GAAGCGGAGT GAACCCCAAC 
     1321 CTGAGGAGGG GAGCCCAGCT GCAGGGCAGA AGGGTGGGGC CCCAGCTGAA GGAGAGGGAA 
     1381 CTGAGACCCC ACCAGAAGCC TCCAGAGCTG TGGAGAATGG CTGCTGTGTG ACCAAGGAAG 
     1441 GCCGTGGAGC CTCTGCAGGA GAGGGCAAAG AACAGAAGCA GACCAACATC GAATCCATGA 
     1501 AAATGGAGGG CTCCCGGGGC CGACTGCGAG GTGGCTTGGG CTGGGAGTCC AGCCTCCGTC 
     1561 AGCGACCCAT GCCAAGACTC ACCTTCCAGG CAGGGGACCC CTACTACATC AGCAAACGGA 
     1621 AACGGGATGA GTGGCTGGCA CGTTGGAAAA GGGAGGCTGA GAAGAAAGCC AAGGTAATTG 
     1681 CAGTAATGAA TGCTGTGGAA GAGAACCAGG CCTCTGGAGA GTCTCAGAAG GTGGAGGAGG 
     1741 CCAGCCCTCC TGCTGTGCAG CAGCCCACGG ACCCTGCTTC TCCGACTGTG GCCACCACCC 
     1801 CTGAGCCAGT AGGAGGGGAT GCTGGGGACA AGAATGCTAC CAAAGCAGCC GACGATGAGC 
     1861 CTGAGTATGA GGATGGCCGG GGCTTTGGCA TTGGAGAGCT GGTGTGGGGG AAACTTCGGG 
     1921 GCTTCTCCTG GTGGCCAGGC CGAATTGTGT CTTGGTGGAT GACAGGCCGG AGCCGAGCAG 
     1981 CTGAAGGCAC TCGCTGGGTC ATGTGGTTCG GAGATGGCAA GTTCTCAGTG GTGTGTGTGG 
     2041 AGAAGCTCAT GCCGCTGAGC TCCTTCTGCA GTGCATTCCA CCAGGCCACC TACAACAAGC 
     2101 AGCCCATGTA CCGCAAAGCC ATCTACGAAG TCCTCCAGGT GGCCAGCAGC CGTGCCGGGA 
     2161 AGCTGTTTCC AGCTTGCCAT GACAGTGATG AAAGTGACAG TGGCAAGGCT GTGGAAGTGC 
     2221 AGAACAAGCA GATGATTGAA TGGGCCCTCG GTGGCTTCCA GCCCTCGGGT CCTAAGGGCC 
     2281 TGGAGCCACC AGAAGAAGAG AAGAATCCTT ACAAGGAAGT TTACACCGAC ATGTGGGTGG 
     2341 AGCCTGAAGC AGCTGCTTAC GCCCCACCCC CACCAGCCAA GAAACCCAGA AAGAGCACAA 
     2401 CAGAGAAACC TAAGGTCAAG GAGATCATTG ATGAGCGCAC AAGGGAGCGG CTGGTGTATG 
     2461 AGGTGCGCCA GAAGTGCAGA AACATCGAGG ACATTTGTAT CTCATGTGGG AGCCTCAATG 
     2521 TCACCCTGGA GCACCCACTC TTCATTGGAG GCATGTGCCA GAACTGTAAG AACTGCTTCT 
     2581 TGGAGTGTGC TTACCAGTAT GACGACGATG GGTACCAGTC CTATTGCACC ATCTGCTGTG 
     2641 GGGGGCGTGA AGTGCTCATG TGTGGGAACA ACAACTGCTG CAGGTGCTTT TGTGTCGAGT 
     2701 GTGTGGATCT CTTGGTGGGG CCAGGAGCTG CTCAGGCAGC CATTAAGGAA GACCCCTGGA 
     2761 ACTGCTACAT GTGCGGGCAT AAGGGCACCT ATGGGCTGCT GCGAAGACGG GAAGACTGGC 
     2821 CTTCTCGACT CCAGATGTTC TTTGCCAATA ACCATGACCA GGAATTTGAC CCCCCAAAGG 
     2881 TTTACCCACC TGTGCCAGCT GAGAAGAGGA AGCCCATCCG CGTGCTGTCT CTCTTTGATG 
     2941 GGATTGCTAC AGGGCTCCTG GTGCTGAAGG ACCTGGGCAT CCAAGTGGAC CGCTACATTG 
     3001 CCTCCGAGGT GTGTGAGGAC TCCATCACGG TGGGCATGGT GCGGCACCAG GGAAAGATCA 
     3061 TGTACGTCGG GGACGTCCGC AGCGTCACAC AGAAGCATAT CCAGGAGTGG GGCCCATTCG 
     3121 ACCTGGTGAT TGGAGGCAGT CCCTGCAATG ACCTCTCCAT TGTCAACCCT GCCCGCAAGG 
     3181 GACTTTATGA GGGTACTGGC CGCCTCTTCT TTGAGTTCTA CCGCCTCCTG CATGATGCGC 
     3241 GGCCCAAGGA GGGAGATGAT CGCCCCTTCT TCTGGCTCTT TGAGAATGTG GTGGCCATGG 
     3301 GCGTTAGTGA CAAGAGGGAC ATCTCGCGAT TTCTTGAGTC TAACCCCGTG ATGATTGACG 
     3361 CCAAAGAAGT GTCTGCTGCA CACAGGGCCC GTTACTTCTG GGGTAACCTT CCTGGCATGA 
     3421 ACAGGCCTTT GGCATCCACT GTGAATGATA AGCTGGAGCT GCAAGAGTGT CTGGAGCACG 
     3481 GCAGAATAGC CAAGTTCAGC AAAGTGAGGA CCATTACCAC CAGGTCAAAC TCTATAAAGC 
     3541 AGGGCAAAGA CCAGCATTTC CCCGTCTTCA TGAACGAGAA GGAGGACATC CTGTGGTGCA 
     3601 CTGAAATGGA AAGGGTGTTT GGCTTCCCCG TCCACTACAC AGACGTCTCC AACATGAGCC 
     3661 GCTTGGCGAG GCAGAGACTG CTGGGCCGAT CGTGGAGCGT GCCGGTCATC CGCCACCTCT 
     3721 TCGCTCCGCT GAAGGAATAT TTTGCTTGTG TGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3781 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3841 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3901 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3961 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4021 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4081 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4141 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4201 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4261 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4321 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4381 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4441 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4501 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4561 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4621 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4681 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4741 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4801 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4861 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4921 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4981 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5041 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5101 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5161 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5221 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5281 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5341 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5401 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5461 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5521 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5581 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5641 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5701 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5761 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5821 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5881 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5941 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6001 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6061 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6121 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6181 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6241 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6301 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6361 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6421 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6481 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6541 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6601 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6661 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6721 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6781 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6841 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6901 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6961 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7021 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7081 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7141 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7201 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7261 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7321 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7381 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7441 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7501 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7561 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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