LOCUS       MR211081                7557 bp    DNA     circular  SYN 
DEFINITION  Actn1 (Myc-DDK-tagged) - Mouse actinin, alpha 1 (Actn1)
ACCESSION   NM_134156
VERSION     NM_134156.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3714..3805 
                     /label="Myc-DDK"  
     misc_feature    3835..4420 
                     /label="PolyA signal"  
     misc_feature    3892..3911 
                     /label="Restriction Enzyme"  
     misc_feature    4696..5368 
                     /label="ColE1"  
     misc_feature    5798..6592 
                     /label="Kan/Neo"  
     misc_feature    6627..6929 
                     /label="SV40 ori"  
     misc_feature    7015..7492 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3696..3702 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3696 
                     /label="Actn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCATTAT GATTCCCAGC AGACCAACGA CTACATGCAG CCTGAAGAGG 
     1081 ACTGGGATCG GGACCTGCTC CTGGATCCGG CCTGGGAGAA GCAGCAGAGG AAGACGTTCA 
     1141 CAGCCTGGTG CAACTCCCAC CTGCGCAAAG CGGGGACACA GATCGAGAAC ATCGAAGAGG 
     1201 ACTTCCGAGA TGGCCTGAAG CTCATGCTGC TCCTGGAGGT CATCTCAGGT GAACGCTTGG 
     1261 CCAAGCCAGA GAGAGGCAAG ATGAGAGTGC ACAAGATCTC CAACGTCAAC AAGGCCTTGG 
     1321 ATTTCATAGC CAGCAAAGGG GTCAAGCTGG TGTCCATCGG AGCTGAAGAA ATTGTGGATG 
     1381 GGAATGTGAA GATGACCCTG GGCATGATCT GGACCATCAT TCTCCGCTTT GCCATCCAGG 
     1441 ACATCTCCGT GGAAGAGACT TCAGCTAAAG AAGGGTTACT CCTGTGGTGT CAGAGGAAGA 
     1501 CAGCCCCATA TAAAAATGTC AACATCCAAA ACTTCCACAT CAGTTGGAAA GATGGTCTTG 
     1561 GTTTCTGTGC CTTGATCCAC CGACACCGGC CCGAGTTGAT TGACTATGGA AAGCTTCGAA 
     1621 AGGATGATCC ACTCACAAAC CTGAACACGG CCTTTGATGT GGCAGAGAGG TTCCTGGACA 
     1681 TCCCTAAGAT GCTGGATGCT GAAGACATCG TCGGAACTGC CCGGCCAGAT GAGAAAGCCA 
     1741 TTATGACTTA TGTGTCTAGC TTCTATCATG CCTTCTCTGG AGCCCAGAAG GCAGAAACAG 
     1801 CAGCCAATCG CATCTGCAAA GTGTTGGCGG TCAATCAAGA GAACGAGCAG CTTATGGAAG 
     1861 ACTATGAGAA GCTGGCCAGT GATCTGTTGG AGTGGATCCG CCGCACCATC CCATGGCTGG 
     1921 AGAATCGGGT GCCTGAGAAC ACCATGCATG CCATGCAGCA GAAGCTGGAG GACTTCCGAG 
     1981 ACTATAGACG CCTGCACAAG CCGCCCAAGG TGCAGGAGAA GTGCCAGCTG GAGATCAACT 
     2041 TTAACACGCT GCAGACCAAG CTGCGGCTCA GCAACCGGCC TGCCTTCATG CCCTCCGAGG 
     2101 GCAGGATGGT CTCGGATATC AACAATGCCT GGGGCTGCCT GGAACAGGCC GAGAAGGGCT 
     2161 ACGAGGAGTG GCTGCTGAAT GAGATCCGGA GGCTAGAGAG GTTGGACCAC CTGGCAGAGA 
     2221 AGTTCCGGCA GAAGGCCTCC ATCCATGAAG CCTGGACGGA TGGCAAAGAA GCCATGCTTC 
     2281 GGCAGAAGGA TTATGAGACA GCCACCCTGT CCGAGATCAA GGCCTTGCTC AAGAAGCACG 
     2341 AGGCTTTTGA GAGTGATCTG GCTGCCCACC AGGACCGCGT GGAGCAGATT GCGGCCATTG 
     2401 CCCAGGAGCT TAATGAGCTG GACTACTATG ACTCGCCAAG TGTCAACGCT CGTTGCCAAA 
     2461 AGATCTGTGA CCAGTGGGAC AATCTAGGGG CTCTCACTCA GAAGCGGAGG GAAGCTTTAG 
     2521 AGCGAACAGA GAAGCTGCTG GAAACCATCG ACCAGCTCTA CTTAGAGTAC GCCAAGAGGG 
     2581 CTGCACCCTT CAACAATTGG ATGGAGGGAG CCATGGAGGA CCTGCAGGAC ACCTTCATCG 
     2641 TACATACCAT CGAGGAGATC CAGGGACTGA CCACAGCCCA TGAGCAGTTC AAGGCCACCC 
     2701 TCCCGGATGC AGACAAGGAG CGCCTCGCCA TTCTGGGCAT CCACAATGAA GTGTCCAAGA 
     2761 TCGTCCAGAC CTATCATGTC AACATGGCAG GCACCAACCC CTATACAACC ATCACGCCTC 
     2821 AGGAGATCAA TGGCAAATGG GACCATGTAC GGCAGCTAGT GCCCCGGAGG GACCAGGCTC 
     2881 TCACAGAGGA ACATGCAAGG CAGCAGCACA ATGAGAGGCT ACGCAAACAG TTTGGCGCAC 
     2941 AAGCCAATGT CATTGGGCCC TGGATCCAGA CCAAGATGGA GGAGATTGGG AGGATTTCCA 
     3001 TTGAGATGCA CGGCACCCTG GAGGACCAAC TCAGCCACCT GCGGCAGTAT GAGAAGAGCA 
     3061 TCGTCAATTA CAAGCCAAAG ATTGACCAGC TGGAGTGCGA CCACCAGCTC ATCCAGGAGG 
     3121 CACTTATCTT TGACAATAAG CACACCAACT ACAACATGGA GCATATCCGT GTGGGCTGGG 
     3181 AGCAGCTGCT CACCACCATT GCCAGGACCA TCAATGAAGT GGAGAACCAG ATCTTGACCC 
     3241 GGGATGCCAA AGGCATCAGC CAGGAACAGA TGAACGAATT CCGCGCCTCT TTCAACCACT 
     3301 TTGACCGGGA TCACTCCGGC ACGTTGGGTC CCGAAGAGTT CAAAGCCTGC CTCATCAGCT 
     3361 TGGGTTATGA TATTGGCAAC GACCCCCAGG GAGAGGCAGA GTTTGCCCGA ATCATGAGCA 
     3421 TTGTAGACCC CAACCGCTTG GGGGTAGTGA CGTTCCAGGC CTTCATCGAC TTTATGTCCC 
     3481 GAGAGACGGC AGATACTGAT ACTGCAGATC AGGTCATGGC TTCCTTCAAG ATCCTGGCTG 
     3541 GAGATAAGAA TTACATCACA GAGGATGAGC TGCGCCGTGA GCTGCCCCCT GACCAAGCCG 
     3601 AGTACTGCAT CGCAAGAATG GCCCCCTACG CGGGCCCCGA CTCCGTGCCC GGTGCTTTAG 
     3661 ACTACATGTC CTTCTCCACG GCACTGTATG GCGAGAGCGA CCTCACGCGT ACGCGGCCGC 
     3721 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3781 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3841 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3901 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3961 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4021 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4081 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4141 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4201 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4261 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4321 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4381 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4441 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4501 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4561 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4621 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4681 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4741 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4801 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4861 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4921 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4981 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5041 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5101 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5161 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5221 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5281 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5341 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5401 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5461 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5521 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5581 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5641 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5701 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5761 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5821 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5881 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5941 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6001 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6061 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6121 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6181 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6241 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6301 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6361 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6421 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6481 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6541 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6601 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6661 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6721 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6781 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6841 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6901 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6961 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7021 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7081 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7141 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7201 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7261 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7321 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7381 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7441 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7501 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//