LOCUS       MR210883                7383 bp    DNA     circular  SYN 
DEFINITION  Trim28 (Myc-DDK-tagged) - Mouse tripartite motif-containing 28 (Trim28)
ACCESSION   NM_011588
VERSION     NM_011588.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3540..3631 
                     /label="Myc-DDK"  
     misc_feature    3661..4246 
                     /label="PolyA signal"  
     misc_feature    3718..3737 
                     /label="Restriction Enzyme"  
     misc_feature    4522..5194 
                     /label="ColE1"  
     misc_feature    5624..6418 
                     /label="Kan/Neo"  
     misc_feature    6453..6755 
                     /label="SV40 ori"  
     misc_feature    6841..7318 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3522..3528 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3522 
                     /label="Trim28"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCTCG GCGGCAGCGA CTGCAGCGGC CTCGGCCGCG ACGGCCGCCT 
     1081 CGGCGGCCTC TGGTAGCCCA GGGTCGGGCG AGGGCTCGGC GGGCGGTGAG AAGCGTCCGG 
     1141 CTGCTTCCTC AGCCGCGGCG GCCTCTGCAG CCGCGTCGTC CCCTGCGGGG GGCGGTGGCG 
     1201 AGGCGCAGGA GCTTCTGGAG CACTGCGGCG TGTGTCGCGA GCGCCTGCGG CCCGAGCGGG 
     1261 ATCCTCGGCT GCTGCCCTGT CTACATTCGG CCTGCAGTGC CTGCCTGGGC CCCGCTACAC 
     1321 CCGCCGCAGC GAATAATTCG GGGGATGGCG GCTCGGCGGG CGACGGCGCT ATGGTGGATT 
     1381 GTCCAGTGTG CAAACAGCAG TGCTACTCCA AAGACATCGT GGAGAATTAT TTTATGCGTG 
     1441 ATAGTGGCAG TAAGGCCTCT TCTGATTCCC AGGATGCTAA CCAGTGCTGC ACTAGCTGTG 
     1501 AAGATAATGC CCCAGCCACT AGCTATTGTG TGGAGTGCTC TGAACCACTT TGTGAGACCT 
     1561 GTGTGGAGGC TCACCAGCGG GTGAAATACA CCAAGGACCA CACTGTGCGC TCCACAGGAC 
     1621 CTGCTAAGAC TCGAGATGGA GAGCGAACAG TCTACTGTAA TGTGCACAAG CATGAGCCCC 
     1681 TCGTGCTGTT CTGTGAGAGC TGTGACACAC TCACCTGCCG CGACTGCCAG CTCAACGCTC 
     1741 ACAAGGACCA TCAGTACCAG TTTTTGGAAG ATGCAGTGAG GAACCAACGT AAACTCTTGG 
     1801 CTTCACTGGT GAAACGTCTT GGGGACAAAC ATGCCACACT TCAGAAAAAC ACCAAGGAGG 
     1861 TTCGAAGCTC GATCCGCCAG GTGTCTGATG TGCAGAAGCG AGTGCAGGTT GATGTCAAGA 
     1921 TGGCCATTCT GCAGATCATG AAGGAGCTGA ATAAGCGGGG TCGAGTTCTG GTCAATGATG 
     1981 CCCAGAAGGT GACCGAGGGT CAGCAGGAAC GTCTGGAGCG CCAGCACTGG ACCATGACCA 
     2041 AAATTCAGAA GCACCAGGAA CACATTTTGC GTTTTGCCTC TTGGGCTCTG GAGAGTGATA 
     2101 ACAATACAGC TCTCTTGCTC TCTAAGAAGC TGATCTATTT CCAGCTGCAT CGGGCCCTCA 
     2161 AAATGATTGT GGATCCTGTG GAGCCTCATG GTGAGATGAA GTTTCAGTGG GATCTCAATG 
     2221 CCTGGACCAA GAGTGCTGAA GCCTTTGGCA AGATTGTGGC TGAGCGTCCT GGTACGAACT 
     2281 CCACAGGTCC TGGGCCCATG GCTCCTCCAA GAGCCCCAGG CCCTCTAAGC AAGCAAGGTT 
     2341 CTGGCAGTAG CCAGCCCATG GAAGTACAAG AGGGATATGG CTTTGGGTCA GATGATCCCT 
     2401 ATTCAAGTGC AGAGCCGCAT GTATCAGGCA TGAAGCGGTC CCGCTCTGGT GAGGGAGAGG 
     2461 TAAGTGGCCT CTTAAGGAAG GTGCCACGTG TGAGCCTTGA ACGCCTGGAT CTGGACCTCA 
     2521 CCTCTGACAG CCAGCCACCA GTCTTCAAGG TCTTTCCTGG AAGCACTACT GAGGACTACA 
     2581 ATCTGATTGT TATTGAGCGT GGTGCTGCTG CAGCAGCTGC TGGTCAGGCT GGGACTGTGC 
     2641 CACCAGGAGC CCCTGGTGCC CCACCCCTTC CTGGCATGGC CATTGTCAAG GAAGAAGAGA 
     2701 CAGAAGCTGC TATTGGAGCT CCCCCGGCTG CCCCCGAGGG TCCTGAAACC AAGCCTGTGT 
     2761 TGATGCCTCT GACTGAAGGT CCTGGTGCCG AGGGACCTCG TCTAGCTTCA CCTAGTGGCA 
     2821 GTACCAGCTC AGGCTTGGAG GTGGTGGCTC CTGAGGTTAC CTCAGCCCCA GTAAGTGGGC 
     2881 CAGGTATCCT GGATGACAGT GCCACTATCT GCCGTGTCTG CCAGAAACCA GGTGACCTGG 
     2941 TCATGTGTAA CCAGTGCGAA TTTTGCTTCC ACCTGGATTG CCACCTCCCT GCCCTGCAGG 
     3001 ATGTTCCAGG GGAGGAATGG AGTTGCTCAC TCTGCCACGT GCTCCCTGAC CTAAAGGAGG 
     3061 AAGATGGAAG CCTCAGCCTG GATGGAGCAG ATAGCACTGG TGTGGTAGCT AAACTCTCAC 
     3121 CAGCCAACCA GCGGAAATGT GAGCGTGTTC TCCTGGCCCT GTTCTGCCAT GAACCATGCC 
     3181 GTCCCTTGCA TCAGCTGGCT ACCGACTCTA CATTCTCCAT GGAGCAGCCT GGTGGTACCC 
     3241 TAGACCTGAC CTTGATTCGT GCTCGCCTCC AAGAGAAGCT GTCACCTCCT TATAGCTCCC 
     3301 CCCAGGAGTT TGCTCAAGAT GTGGGCCGCA TGTTCAAACA GTTCAACAAG CTGACTGAGG 
     3361 ACAAGGCAGA TGTTCAGTCC ATCATCGGCT TGCAGCGCTT CTTTGAGACA CGCATGAATG 
     3421 ATGCCTTTGG TGACACCAAG TTTTCTGCTG TGCTGGTAGA ACCACCACCA TTGAACCTTC 
     3481 CCAGTGCTGG CCTAAGTTCT CAGGAGCTCT CTGGCCCTGG TGATGGCCCC ACGCGTACGC 
     3541 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3601 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3661 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3721 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3781 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3841 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3901 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     3961 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4021 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4081 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4141 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4201 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4261 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4321 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4381 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4441 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4501 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4561 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4621 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4681 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4741 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4801 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4861 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4921 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     4981 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5041 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5101 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5161 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5221 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5281 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5341 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5401 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5461 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5521 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5581 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5641 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5701 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5761 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5821 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5881 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     5941 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6001 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6061 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6121 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6181 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6241 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6301 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6361 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6421 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6481 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6541 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6601 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6661 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6721 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6781 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6841 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6901 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     6961 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7021 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7081 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7141 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7201 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7261 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7321 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7381 TTT
//