LOCUS       MR210420                7114 bp    DNA     circular  SYN 
DEFINITION  Col8a1 (Myc-DDK-tagged) - Mouse collagen, type VIII, alpha 1 (Col8a1)
ACCESSION   NM_007739
VERSION     NM_007739.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3271..3362 
                     /label="Myc-DDK"  
     misc_feature    3392..3977 
                     /label="PolyA signal"  
     misc_feature    3449..3468 
                     /label="Restriction Enzyme"  
     misc_feature    4253..4925 
                     /label="ColE1"  
     misc_feature    5355..6149 
                     /label="Kan/Neo"  
     misc_feature    6184..6486 
                     /label="SV40 ori"  
     misc_feature    6572..7049 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3253..3259 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3253 
                     /label="Col8a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACTGCCA CCCAGGCCTC TACAGCTGCT GGGAATACTG TTCATCATTT 
     1081 CCCTGAACTC TGTCAGACTC ATTCAGGCCG GTGCCTACTA TGGAATCAAG CCTCTGCCAC 
     1141 CTCAAATCCC TCCTCAGATA CCACCACAAA TTCCACAGTA CCAGCCCTTG GGCCAGCAAG 
     1201 TCCCTCACAT GCCTTTGGGC AAAGATGGCC TTTCCATGGG CAAGGAGATG CCTCACATGC 
     1261 AGTATGGCAA AGAGTACCCA CACCTACCCC AATATATGAA GGAAATCCCA CCTGTGCCAA 
     1321 GAATGGGCAA GGAAGTGGTG CCCAAAAAAG GCAAAGGAGA AGTACCGTTA GCCAGTTTGC 
     1381 GCGGAGAACA AGGTCCCCGT GGAGAACCTG GACCAAGAGG ACCACCTGGG CCGCCAGGTT 
     1441 TACCAGGTCA TGGAATGCCT GGAATCAAAG GAAAACCAGG GCCCCAGGGA TATCCAGGAA 
     1501 TTGGAAAGCC CGGTATGCCT GGAATGCCTG GGAAGCCAGG AGCCATGGGA ATGCCAGGGG 
     1561 CAAAAGGCGA AATTGGACCC AAAGGGGAAA TCGGACCTAT GGGAATCCCA GGGCCACAAG 
     1621 GGCCTCCAGG ACCTCATGGA CTTCCTGGCA TCGGGAAACC GGGTGGGCCA GGGTTACCAG 
     1681 GGCAACCAGG AGCAAAAGGT GAGAGGGGGC CCAAAGGACC ACCAGGACCT CCCGGCCTTC 
     1741 AGGGTCCCAA AGGAGAGAAG GGCTTTGGGA TGCCTGGCTT GCCAGGTCTG AAGGGTCCTC 
     1801 CAGGTATGCA TGGCCCTCCT GGACCAGTTG GACTACCGGG AGTAGGAAAA CCAGGAGTGA 
     1861 CAGGCTTTCC TGGACCACAG GGTCCCCTGG GGAAGCCAGG GCCTCCAGGG GAACCTGGAC 
     1921 CACAAGGCCT TATTGGTGTG CCAGGAGTTC AAGGACCTCC TGGGATGCCT GGAGTTGGAA 
     1981 AGCCAGGTCA GGATGGGATC CCGGGCCAAC CAGGATTTCC GGGTGGTAAA GGGGAACAAG 
     2041 GATTGCCAGG GTTGCCTGGA CCCCCAGGCC TCCCAGGGGT CGGAAAGCCA GGTTTCCCAG 
     2101 GACCCAAAGG GGACCGGGGT ATTGGGGGTG TTCCTGGGGT TCTTGGGCCG AGAGGGGAAA 
     2161 AAGGACCCAT TGGTGCTCCT GGAATGGGGG GTCCACCTGG AGAGCCAGGC CTACCCGGTA 
     2221 TCCCGGGTCC CATGGGCCCT CCAGGTGCTA TTGGTTTCCC TGGACCCAAA GGAGAAGGTG 
     2281 GAGTTGTAGG ACCACAGGGT CCACCAGGCC CCAAGGGAGA GCCTGGCCTC CAAGGCTTCC 
     2341 CTGGGAAGCC AGGTTTTCTT GGTGAAGTAG GTCCCCCTGG CATGAGGGGT TTGCCTGGTC 
     2401 CTATAGGACC CAAGGGAGAA GGTGGTCACA AAGGGTTGCC AGGGCTTCCC GGTGTTCCAG 
     2461 GGCTGCTTGG ACCCAAAGGA GAACCTGGCA TACCTGGGGA TCAGGGTCTA CAGGGTCCCC 
     2521 CAGGGATTCC AGGGATCGTA GGACCTAGTG GCCCTATTGG ACCCCCTGGG ATTCCGGGCC 
     2581 CCAAAGGAGA ACCAGGCCTC CCAGGGCCCC CTGGGTTCCC TGGTGTAGGG AAGCCAGGAG 
     2641 TAGCAGGACT TCATGGGCCC CCGGGGAAAC CTGGTGCCCT TGGGCCTCAA GGCCAGCCTG 
     2701 GCCTTCCTGG ACCCCCAGGT CCTCCAGGAC CCCCAGGCCC TCCAGCTGTG ATGCCTACAC 
     2761 CATCACCCCA GGGAGAGTAT CTGCCAGATA TGGGACTAGG AATTGATGGG GTGAAACCTC 
     2821 CGCATGCCTA TGCGGGCAAA AAGGGCAAAC ACGGAGGGCC AGCCTATGAG ATGCCTGCGT 
     2881 TTACTGCCGA GCTGACTGTA CCTTTCCCAC CGGTGGGGGC CCCAGTGAAG TTTGACAAGC 
     2941 TGCTCTACAA CGGCAGACAG AACTACAATC CGCAGACAGG CATCTTCACC TGTGAAGTCC 
     3001 CGGGTGTCTA CTACTTTGCT TATCATGTTC ACTGCAAGGG AGGCAACGTA TGGGTTGCTC 
     3061 TCTTCAAGAA CAACGAGCCC ATGATGTACA CATACGACGA GTACAAGAAG GGCTTTCTGG 
     3121 ACCAAGCATC TGGAAGCGCA GTACTGCTTC TCCGTCCCGG AGACCAGGTG TTTCTCCAAA 
     3181 TGCCTTCAGA ACAGGCTGCT GGACTCTATG CCGGGCAATA TGTCCACTCC TCCTTTTCAG 
     3241 GATATTTATT GTATCCCATG TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA 
     3301 GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG 
     3361 GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC 
     3421 CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT 
     3481 AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG 
     3541 GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA 
     3601 TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG 
     3661 GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC 
     3721 CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT 
     3781 GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT 
     3841 ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA 
     3901 GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG 
     3961 TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT 
     4021 GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     4081 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     4141 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     4201 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     4261 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     4321 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     4381 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     4441 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     4501 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     4561 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     4621 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     4681 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     4741 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     4801 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     4861 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG 
     4921 CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG 
     4981 GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG 
     5041 GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA 
     5101 CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG 
     5161 TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT 
     5221 CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT 
     5281 CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG 
     5341 TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT 
     5401 GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC 
     5461 GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC 
     5521 ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG 
     5581 CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG 
     5641 CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC 
     5701 GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC 
     5761 GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA 
     5821 TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA 
     5881 TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC 
     5941 CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA 
     6001 CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC 
     6061 ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC 
     6121 GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT 
     6181 CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA 
     6241 ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT 
     6301 GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG 
     6361 GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC 
     6421 CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC 
     6481 CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT 
     6541 TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 
     6601 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 
     6661 ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 
     6721 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 
     6781 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 
     6841 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 
     6901 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT 
     6961 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT 
     7021 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA 
     7081 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//