LOCUS       MR210235                7038 bp    DNA     circular  SYN 
DEFINITION  G2e3 (Myc-DDK-tagged) - Mouse G2/M-phase specific E3 ubiquitin ligase (G2e3), transcript variant 1
ACCESSION   NM_001015099
VERSION     NM_001015099.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3193..3284 
                     /label="Myc-DDK"  
     misc_feature    3314..3899 
                     /label="PolyA signal"  
     misc_feature    3371..3390 
                     /label="Restriction Enzyme"  
     misc_feature    4175..4847 
                     /label="ColE1"  
     misc_feature    5277..6071 
                     /label="Kan/Neo"  
     misc_feature    6106..6408 
                     /label="SV40 ori"  
     misc_feature    6494..6971 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3168..3175 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3168 
                     /label="G2e3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATGAAAAT AAACCTGATA ATTCACAAAG TCTTGCTTGT GTTTTCTGCC 
     1081 GAAAAAATGA TGACTGTCCC AATAAATACG GAGAAAAGAA AACATATGAA AAATGGAATT 
     1141 TTAGTGTCCA CTACTACTGC TTGTTAATGT CAAGTGGAAT TTGGCAGAGA GGTAAAGAAG 
     1201 AAGAAGGAGT TTATGGCTTC CTAATAGAAG ACATCAGGAA GGAAGTCCAG AGAGCCTCCA 
     1261 AACTGAAGTG TACAGTTTGC AAGAAGAATG GTGCTTCAAT TGGATGTGTG GTACCCACGT 
     1321 GTAAACGAAG TTATCACTTG CCTTGTGGAC TTCAGAAAGA ATGTATTTTC CAGTTCACTG 
     1381 ACAATTTTGC GTCATTTTGC TGGAAACATC GACCCGTTCA AGCAATTACA TCTAATAAAT 
     1441 ATAGTAGTTC TTTACCATGT ACTATCTGTT TGGAATTTGT CGAGCCTATT CCTACTTATA 
     1501 ACATACTACA AAGTCCCTGT TGTAAGAATG CTTGGTTTCA TAGAGACTGT TTACAGGTCC 
     1561 AAGCAATAAA TGCAGGAGTG TTTTTCTTTA GATGTACACT ATGCAATAAT ACAGACATCT 
     1621 TTCAGAAGGA GATGTTGAGA ATGGGAATTC ATATTCCTGA AAAAGATGCA TCCTGGGAAT 
     1681 TAGAAGAAAA CGCGTATCAA GAGCTGCTGC AGAGCCACGA CCGCTGTGAT ATTCGAAGAT 
     1741 GTCACTGCAA AAAAGGCCGA GACTACAACG AACCTAACAG CAAATGGGAA GTAAAGCGGT 
     1801 GTCAGAGTTG TGGTTCTAGT GGAACACACT TAGCCTGTTC CTCACTACAG TCTTGGGAAC 
     1861 AAAATTGGGA GTGTTTGGAC TGTAGAAGAA TTACCTACAC TTCAGATTTC CAAAAGGCCC 
     1921 CCAAACACCC ATTAGCCAAC TCTACTAATG TGACAGTTAC TGACTGCTTG TTGGAAGAGT 
     1981 CATCATCTAA GCTCCCCAGA CAGTCAACTG TAGCCCAGCA TAAAGAACTA CTGAGGCAAG 
     2041 GCAGCAAATT TAGGAGAGAC ATTTCAACAA TATTGATAGA GTTAGGATTC CAAATTAAAA 
     2101 AGAAAACTAA GACATTATAC ATCAACAAAG CCAATGTGTG GAGGAGTGCC TTAGAGCAGT 
     2161 TCCAGAGCCA GAAGTTTAAC CCTTCATGCT CCATTGACGT AGTGTATGTT AATGGCAATG 
     2221 AGGTTGGAAG CCAGCATCTT GGATCAAAGC AGGAATTTCT GAGTCACTTA ATGCACCATC 
     2281 TTGAGAATTC ATCAGTGTTT GAAGGGTCCT TGGCAAAGAA CTTGTCTCTA AATTCTCAAG 
     2341 CTGTGAAAGA GAATCTTTAC TATGAAGTTG GCAAAATGCT TGCCATTTCT TTAGTTCATG 
     2401 GTGGTCCTTC GCCTGGTTTC TTTTCTGAAA CCTTGTTTAA CTGCCTTGCT TATGGACCAG 
     2461 AAAATACTCT ACCAACTTTG GATGATGTAT CAGACATTGA TGTTGCACAG ATTATAATCA 
     2521 AGATAGATTC TGCAACTGAT TTAAACATTT TAAATTCAGT AATAAGCCAA CACTATAACT 
     2581 ACTTAGAGGT CAGTGGATGT TTAAGACTTA CGACATCATT AAGTGATAAA TTCATGTTGG 
     2641 TAAAAGACAT CCTTTTCTAC CATGTAATTA ATAGAGTCAA GGCACCTTTT GAGAGTTTTA 
     2701 AACAGGGTCT GAAAACGCTT GGTGTTTTAG AGAAAATTCA AACTTACCCA GAAGCATTTT 
     2761 ATAAGATCCT ATGTCATAAA CCTGAGAATC TCTCTGCAAA AAACCTCAGT GACCTCTTCA 
     2821 CAATTCACTC GGTAGCGGAC GTACAGACTT TGAGGTTTTG GAACAGTTAC TTAAAGGCTA 
     2881 TAGAGGATGG TAAATCTGCA ACAACGATGG AAGATATTCT TATTTTTGCA ACTGGCTGTA 
     2941 GTTCTGTCCC ACCAACTGGA TTTAAACCCT CTCTGTCAGT TGAATGTCTA CATGTGGATT 
     3001 TTCCTGTTGC AGACAAATAT AGAAACCATT TAGTTCTACC AGCCACCAAC ACGTATGAAG 
     3061 AGTTCCAAGA AAATATGGAT TTCACCATAA GAGACACTCT AAGATTAGAA AAGGAAGAAA 
     3121 GGTCTCACAT ACTACCTCGG ACATTAAATG TTTCTTCTAA TGAAGAAATG CTTATTAGCG 
     3181 GACCGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3241 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3301 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3361 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3421 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3481 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3541 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3601 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3661 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3721 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3781 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3841 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     3901 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     3961 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4021 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4081 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4141 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4201 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4261 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4321 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4381 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4441 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4501 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4561 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4621 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4681 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4741 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4801 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4861 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     4921 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     4981 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5041 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5101 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5161 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5221 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5281 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5341 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5401 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5461 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5521 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5581 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5641 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5701 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5761 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5821 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     5881 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     5941 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6001 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6061 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6121 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6181 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6241 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6301 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6361 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6421 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6481 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6541 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6601 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6661 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6721 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6781 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6841 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     6901 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     6961 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7021 AAATTTAACG CGAATTTT
//